Mutagenetix > Incidental Mutations

Incidental Mutation 'R1781:Olfr1351'

195302

Institutional Source

Beutler Lab

Gene Symbol

Olfr1351

Ensembl Gene

ENSMUSG00000063216

Gene Name

olfactory receptor 1351

Synonyms

MOR139-4, GA_x6K02T2QGN0-2794907-2793948

MMRRC Submission

039812-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.175) question?

Stock #

R1781 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

79012472-79019645 bp(+) (GRCm38)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

T to C at 79017325 bp

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 1 (M1T)

Ref Sequence

ENSEMBL: ENSMUSP00000149241 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080730] [ENSMUST00000203906] [ENSMUST00000216030]

Predicted Effect

probably null
Transcript: ENSMUST00000080730
AA Change: M1T

PolyPhen 2 Score 0.892 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000079555
Gene: ENSMUSG00000063216
AA Change: M1T

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
Pfam:7tm_4	32	308	2e-49	PFAM
Pfam:7tm_1	42	291	1.9e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203906
AA Change: M1T

SMART Domains

Protein: ENSMUSP00000144814
Gene: ENSMUSG00000060205
AA Change: M1T

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
Pfam:7tm_4	32	308	2e-49	PFAM
Pfam:7tm_1	42	291	1.9e-23	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216030
AA Change: M1T

PolyPhen 2 Score 0.892 (Sensitivity: 0.82; Specificity: 0.94)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219714

Meta Mutation Damage Score

0.8998

Coding Region Coverage

1x: 97.3%
3x: 96.7%
10x: 94.6%
20x: 90.1%

Validation Efficiency

97% (88/91)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430078G23Rik	C	T	8: 3,380,495	R123W	probably damaging	Het
Aadacl2	A	G	3: 60,024,696	K211E	probably damaging	Het
Abca13	T	A	11: 9,269,194	L376Q	probably damaging	Het
Abca17	T	C	17: 24,267,557	T1499A	possibly damaging	Het
Anks6	A	C	4: 47,043,639	N363K	possibly damaging	Het
Apob	T	A	12: 8,009,603	I2695N	possibly damaging	Het
Atg14	T	A	14: 47,549,150		probably null	Het
Atg2a	A	T	19: 6,256,213	I1368F	probably damaging	Het
Btbd9	A	G	17: 30,513,593	S373P	probably damaging	Het
Ccdc83	T	C	7: 90,250,541	E41G	probably damaging	Het
Ccnh	T	C	13: 85,206,135	S233P	possibly damaging	Het
Cdh8	T	C	8: 99,190,462		probably null	Het
Cdh8	A	T	8: 99,279,658	I99N	probably damaging	Het
Cdr2	G	A	7: 120,958,045	P419L	probably benign	Het
Cds1	T	A	5: 101,812,550	I289K	possibly damaging	Het
Cherp	G	A	8: 72,467,771	T394I	probably damaging	Het
Cyp4f17	T	G	17: 32,524,019	I222S	possibly damaging	Het
Dcc	A	G	18: 71,378,717	S856P	probably benign	Het
Dcp1a	C	T	14: 30,513,075	T221I	probably benign	Het
Ddx10	A	G	9: 53,207,545	S475P	probably damaging	Het
Dennd5b	G	T	6: 149,027,398	A759E	probably damaging	Het
Disp2	G	A	2: 118,792,561	G1258D	probably damaging	Het
Fam208b	T	C	13: 3,584,759	T683A	possibly damaging	Het
Fhdc1	A	T	3: 84,448,804	D444E	probably damaging	Het
Fhod1	T	C	8: 105,347,789		probably benign	Het
Gcnt7	T	C	2: 172,454,880	K8R	probably benign	Het
Gk5	C	T	9: 96,133,455	T108I	possibly damaging	Het
Gm8214	C	A	1: 183,681,932		noncoding transcript	Het
Gnl1	A	T	17: 35,987,746	I434F	probably damaging	Het
Golga2	T	C	2: 32,306,576	Y986H	probably damaging	Het
Gpr21	T	C	2: 37,517,538	V32A	probably benign	Het
Grb14	A	T	2: 64,975,555		probably null	Het
H6pd	A	T	4: 149,995,931	F144L	probably damaging	Het
Hunk	A	G	16: 90,432,560	Y27C	probably damaging	Het
Ints7	C	T	1: 191,596,284	T223M	possibly damaging	Het
Kcnmb2	T	C	3: 32,179,003		probably null	Het
Lilra6	A	T	7: 3,915,067	L26H	probably benign	Het
Mc4r	A	T	18: 66,859,847	V65E	probably damaging	Het
Mgam	G	A	6: 40,669,863	G708R	probably damaging	Het
Mllt6	A	G	11: 97,672,569	D326G	probably benign	Het
Myo7a	A	T	7: 98,073,124	V1198D	probably damaging	Het
Nampt	T	C	12: 32,833,038	V74A	probably damaging	Het
Nlrp4b	G	C	7: 10,715,339	V123L	probably benign	Het
Ntpcr	T	A	8: 125,745,402	L150Q	probably damaging	Het
Obscn	C	T	11: 59,106,337	E1513K	probably damaging	Het
Ola1	T	C	2: 73,156,755	K178E	possibly damaging	Het
Olfr1364	C	T	13: 21,573,541	G305D	probably damaging	Het
Olfr1368	C	T	13: 21,142,764	V98I	probably benign	Het
Olfr139	A	T	11: 74,044,960	F105I	probably damaging	Het
Olfr486	A	G	7: 108,171,883	V287A	probably benign	Het
Olfr53	A	T	7: 140,652,506	I176F	probably damaging	Het
Olfr615	C	T	7: 103,560,566	P30S	probably benign	Het
Olfr629	T	C	7: 103,740,821	M140V	probably benign	Het
Olfr672	A	T	7: 104,996,108	F265L	possibly damaging	Het
Olfr981	A	G	9: 40,023,245	N284S	probably damaging	Het
Opalin	T	C	19: 41,067,631		probably null	Het
P2ry12	A	G	3: 59,217,778	F159L	probably benign	Het
Paqr7	T	C	4: 134,507,281		probably null	Het
Parp1	T	C	1: 180,588,013	S466P	probably benign	Het
Parp4	T	A	14: 56,627,381		probably null	Het
Pcdh1	T	C	18: 38,189,924	D952G	probably damaging	Het
Pde6c	T	C	19: 38,151,698	S336P	possibly damaging	Het
Pgk2	T	C	17: 40,208,507	D10G	probably benign	Het
Phf20l1	A	G	15: 66,632,825	T771A	probably damaging	Het
Pkdrej	A	G	15: 85,821,171	V188A	possibly damaging	Het
Plekhm1	A	G	11: 103,394,856	L251P	probably damaging	Het
Prex2	A	T	1: 11,199,955	N1288I	probably benign	Het
Sarnp	T	C	10: 128,833,322	L16P	probably damaging	Het
Scgb3a2	T	C	18: 43,766,968		probably benign	Het
Scn11a	A	T	9: 119,755,082	I1489N	probably damaging	Het
Scn3a	A	T	2: 65,472,385	L1239Q	probably damaging	Het
Shisa9	T	C	16: 12,267,657	S377P	probably benign	Het
Slc22a30	T	A	19: 8,335,772	T550S	probably damaging	Het
Slc25a11	T	C	11: 70,644,825	T296A	probably benign	Het
Slc35f1	T	A	10: 53,062,436		probably null	Het
Slc6a13	A	G	6: 121,334,852	E396G	probably damaging	Het
Spata31d1c	T	C	13: 65,036,171	I509T	probably benign	Het
Srsf9	C	A	5: 115,327,422	Y9*	probably null	Het
Tbx20	T	C	9: 24,725,499	I431V	probably benign	Het
Tespa1	G	A	10: 130,348,250	G67S	probably benign	Het
Trhde	A	T	10: 114,588,500	V460E	possibly damaging	Het
Trip12	A	T	1: 84,730,621	F1739I	probably benign	Het
Ugcg	C	T	4: 59,207,798	P46S	probably benign	Het
Umodl1	G	A	17: 30,968,550	R196H	probably damaging	Het
Vezf1	G	T	11: 88,081,621	M269I	probably benign	Het
Vmn1r35	A	T	6: 66,679,566	M40K	probably benign	Het
Vmn1r9	G	T	6: 57,071,315	C125F	probably benign	Het
Vmn2r12	C	A	5: 109,091,728	G323V	probably benign	Het
Vmn2r79	A	T	7: 87,002,347	H318L	probably benign	Het
Zfhx3	T	A	8: 108,793,535	S430T	probably benign	Het

Other mutations in Olfr1351

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00913:Olfr1351	APN	10	79018251	missense	probably benign	0.00
IGL01833:Olfr1351	APN	10	79017936	missense	probably benign
IGL02141:Olfr1351	APN	10	79017721	missense	probably damaging	1.00
IGL02178:Olfr1351	APN	10	79017720	missense	possibly damaging	0.59
R1070:Olfr1351	UTSW	10	79017850	missense	probably damaging	0.99
R1631:Olfr1351	UTSW	10	79017405	missense	probably benign	0.00
R1646:Olfr1351	UTSW	10	79017506	nonsense	probably null
R2358:Olfr1351	UTSW	10	79018188	missense	probably damaging	1.00
R3161:Olfr1351	UTSW	10	79017604	missense	probably benign
R3162:Olfr1351	UTSW	10	79017604	missense	probably benign
R3162:Olfr1351	UTSW	10	79017604	missense	probably benign
R5874:Olfr1351	UTSW	10	79017357	missense	possibly damaging	0.95
R6382:Olfr1351	UTSW	10	79017517	missense	probably damaging	1.00
R7108:Olfr1351	UTSW	10	79017649	nonsense	probably null
R7567:Olfr1351	UTSW	10	79017545	missense	probably benign	0.04
R7568:Olfr1351	UTSW	10	79017507	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACTATTACAATTCAACCTTGCCCATCCC -3'
(R):5'- GCAGATGTCCACAAAGGACAGATTAGAG -3'

Sequencing Primer
(F):5'- ggatggaaaagagtggaaaagag -3'
(R):5'- GTACATGGGTGTGTGCAGG -3'

Posted On

2014-05-23