Mutagenetix > Incidental Mutation

Incidental Mutation 'R0079:Zfp341'

19594

Institutional Source

Beutler Lab

Gene Symbol

Zfp341

Ensembl Gene

ENSMUSG00000059842

Gene Name

zinc finger protein 341

Synonyms

MMRRC Submission

038366-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0079 (G1)

Quality Score

210

Status

Validated

Chromosome

Chromosomal Location

154455217-154488741 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 154466914 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 94 (Y94*)

Ref Sequence

ENSEMBL: ENSMUSP00000105324 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081926] [ENSMUST00000109702] [ENSMUST00000126421]

AlphaFold

Q6PGC9

Predicted Effect

probably null
Transcript: ENSMUST00000081926
AA Change: Y94*

SMART Domains

Protein: ENSMUSP00000080596
Gene: ENSMUSG00000059842
AA Change: Y94*

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	320	342	3.69e-4	SMART
ZnF_C2H2	348	370	1.04e-3	SMART
ZnF_C2H2	442	464	1.45e-2	SMART
ZnF_C2H2	470	494	2.17e-1	SMART
ZnF_C2H2	500	522	2.91e-2	SMART
ZnF_C2H2	537	561	1.23e0	SMART
ZnF_C2H2	563	585	3.58e-2	SMART
ZnF_C2H2	591	613	1.62e0	SMART
ZnF_C2H2	619	641	2.27e-4	SMART
ZnF_C2H2	647	674	7.29e0	SMART
ZnF_C2H2	680	702	5.14e-3	SMART
low complexity region	740	759	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109702
AA Change: Y94*

SMART Domains

Protein: ENSMUSP00000105324
Gene: ENSMUSG00000059842
AA Change: Y94*

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	313	335	3.69e-4	SMART
ZnF_C2H2	341	363	1.04e-3	SMART
ZnF_C2H2	435	457	1.45e-2	SMART
ZnF_C2H2	463	487	2.17e-1	SMART
ZnF_C2H2	493	515	2.91e-2	SMART
ZnF_C2H2	530	554	1.23e0	SMART
ZnF_C2H2	556	578	3.58e-2	SMART
ZnF_C2H2	584	606	1.62e0	SMART
ZnF_C2H2	612	634	2.27e-4	SMART
ZnF_C2H2	640	667	7.29e0	SMART
ZnF_C2H2	673	695	5.14e-3	SMART
low complexity region	733	752	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000126421

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 98.8%
3x: 97.8%
10x: 95.4%
20x: 90.7%

Validation Efficiency

78% (155/199)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410004P03Rik	T	A	12: 17,057,183 (GRCm39)	T105S	possibly damaging	Het
Abca13	G	A	11: 9,243,493 (GRCm39)	M1785I	probably benign	Het
Adamts3	G	C	5: 89,840,912 (GRCm39)	P804A	probably benign	Het
Ahrr	G	A	13: 74,431,143 (GRCm39)		probably benign	Het
Ank2	G	T	3: 126,728,264 (GRCm39)	D776E	probably benign	Het
Cep152	T	A	2: 125,460,373 (GRCm39)	K193M	possibly damaging	Het
Cep55	C	T	19: 38,048,769 (GRCm39)	L142F	probably benign	Het
Chd5	A	G	4: 152,470,206 (GRCm39)	Y1884C	probably damaging	Het
Clasp1	T	C	1: 118,471,034 (GRCm39)	L890P	probably damaging	Het
Cul9	C	A	17: 46,848,589 (GRCm39)	E716*	probably null	Het
Cytip	T	A	2: 58,050,006 (GRCm39)	D21V	probably benign	Het
Denr	T	G	5: 124,062,908 (GRCm39)	F137C	probably damaging	Het
Dhrs3	A	T	4: 144,646,618 (GRCm39)	S197C	probably damaging	Het
Egr4	A	T	6: 85,489,751 (GRCm39)	M103K	probably damaging	Het
Eif4enif1	C	T	11: 3,192,676 (GRCm39)	Q835*	probably null	Het
Gckr	A	G	5: 31,463,883 (GRCm39)	I268V	probably benign	Het
Glt6d1	C	A	2: 25,684,739 (GRCm39)		probably null	Het
Hpd	T	C	5: 123,319,544 (GRCm39)	Y8C	probably damaging	Het
Il12rb2	A	T	6: 67,338,889 (GRCm39)	F16I	probably benign	Het
Ildr2	A	T	1: 166,135,289 (GRCm39)	Y347F	probably damaging	Het
Kcnv1	T	C	15: 44,976,729 (GRCm39)	D186G	probably damaging	Het
Khdrbs2	A	G	1: 32,558,996 (GRCm39)		probably null	Het
L1cam	G	T	X: 72,913,364 (GRCm39)	P16H	probably damaging	Het
Lyn	A	G	4: 3,746,768 (GRCm39)	H161R	probably damaging	Het
Mctp2	T	A	7: 71,863,864 (GRCm39)		probably benign	Het
Miox	C	T	15: 89,220,477 (GRCm39)	L189F	possibly damaging	Het
Mitf	A	C	6: 97,973,401 (GRCm39)	M220L	probably benign	Het
Mrpl21	T	C	19: 3,334,807 (GRCm39)	Y50H	possibly damaging	Het
Myh1	T	A	11: 67,104,237 (GRCm39)	L968Q	probably damaging	Het
Myo3b	A	G	2: 69,925,502 (GRCm39)	K18E	possibly damaging	Het
Ncoa6	TGC	TGCGC	2: 155,250,211 (GRCm39)		probably null	Het
Nlrp2	T	A	7: 5,330,729 (GRCm39)	T556S	possibly damaging	Het
Nsmf	T	C	2: 24,949,096 (GRCm39)		probably benign	Het
Nsun7	C	T	5: 66,452,856 (GRCm39)	P558S	probably benign	Het
Or10h5	G	A	17: 33,435,079 (GRCm39)	R80C	probably benign	Het
Or1e34	T	G	11: 73,778,563 (GRCm39)	I212L	probably benign	Het
Or56a3	T	C	7: 104,735,135 (GRCm39)	S71P	probably damaging	Het
Or5d40	A	T	2: 88,015,698 (GRCm39)	Y159F	possibly damaging	Het
Phf19	T	C	2: 34,785,966 (GRCm39)	N501S	probably benign	Het
Ranbp17	A	C	11: 33,450,682 (GRCm39)	I85S	probably damaging	Het
Robo1	A	G	16: 72,730,230 (GRCm39)		probably benign	Het
Sntg1	A	G	1: 8,749,286 (GRCm39)		probably benign	Het
Snx15	A	G	19: 6,173,943 (GRCm39)	L58P	probably damaging	Het
Spink5	G	T	18: 44,110,831 (GRCm39)	C134F	probably damaging	Het
Strip2	T	C	6: 29,920,532 (GRCm39)		probably null	Het
Taf1d	C	T	9: 15,221,240 (GRCm39)	A182V	probably benign	Het
Tenm3	A	G	8: 48,796,380 (GRCm39)	V475A	possibly damaging	Het
Tent4b	T	A	8: 88,926,631 (GRCm39)	Y14N	possibly damaging	Het
Tgds	A	T	14: 118,353,647 (GRCm39)	H223Q	possibly damaging	Het
Thoc2	G	T	X: 40,952,985 (GRCm39)	S230Y	probably benign	Het
Tm9sf4	T	A	2: 153,033,065 (GRCm39)	V290E	probably damaging	Het
Trak2	A	T	1: 58,965,883 (GRCm39)	L97Q	probably damaging	Het
Trnau1ap	A	G	4: 132,041,656 (GRCm39)	Y145H	probably damaging	Het
Vars2	A	T	17: 35,970,048 (GRCm39)	D780E	probably damaging	Het
Vmn1r170	T	A	7: 23,305,735 (GRCm39)	S46T	possibly damaging	Het
Vmn1r20	A	T	6: 57,408,777 (GRCm39)	R34S	possibly damaging	Het
Vmn1r28	G	A	6: 58,242,702 (GRCm39)	A182T	probably benign	Het
Wdr64	T	C	1: 175,622,668 (GRCm39)	M805T	probably benign	Het
Xdh	G	A	17: 74,198,213 (GRCm39)	R1225C	probably damaging	Het
Zfp641	T	C	15: 98,186,970 (GRCm39)	N218D	probably benign	Het
Zscan22	T	C	7: 12,638,014 (GRCm39)		probably null	Het

Other mutations in Zfp341

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00499:Zfp341	APN	2	154,476,151 (GRCm39)	missense	probably damaging	1.00
IGL01352:Zfp341	APN	2	154,470,816 (GRCm39)	missense	probably benign	0.00
IGL01748:Zfp341	APN	2	154,470,847 (GRCm39)	missense	probably damaging	0.99
IGL02260:Zfp341	APN	2	154,483,969 (GRCm39)	missense	possibly damaging	0.89
IGL02329:Zfp341	APN	2	154,474,224 (GRCm39)	missense	possibly damaging	0.90
casanova_grimbacher	UTSW	2	154,466,801 (GRCm39)	missense	probably damaging	1.00
Specious	UTSW	2	154,488,054 (GRCm39)	missense	possibly damaging	0.63
R0570:Zfp341	UTSW	2	154,487,988 (GRCm39)	missense	probably benign	0.02
R0620:Zfp341	UTSW	2	154,476,193 (GRCm39)	missense	possibly damaging	0.94
R1018:Zfp341	UTSW	2	154,487,972 (GRCm39)	missense	probably damaging	1.00
R1611:Zfp341	UTSW	2	154,487,623 (GRCm39)	missense	probably damaging	1.00
R1733:Zfp341	UTSW	2	154,483,298 (GRCm39)	missense	probably benign	0.19
R1822:Zfp341	UTSW	2	154,488,054 (GRCm39)	missense	possibly damaging	0.63
R1956:Zfp341	UTSW	2	154,480,132 (GRCm39)	missense	probably benign	0.09
R2437:Zfp341	UTSW	2	154,470,721 (GRCm39)	missense	probably damaging	0.97
R3623:Zfp341	UTSW	2	154,466,801 (GRCm39)	missense	probably damaging	1.00
R4417:Zfp341	UTSW	2	154,470,907 (GRCm39)	missense	possibly damaging	0.94
R4806:Zfp341	UTSW	2	154,487,786 (GRCm39)	utr 3 prime	probably benign
R4807:Zfp341	UTSW	2	154,487,786 (GRCm39)	utr 3 prime	probably benign
R4863:Zfp341	UTSW	2	154,487,786 (GRCm39)	utr 3 prime	probably benign
R4955:Zfp341	UTSW	2	154,479,950 (GRCm39)	missense	probably damaging	0.98
R4962:Zfp341	UTSW	2	154,468,734 (GRCm39)	missense	possibly damaging	0.80
R5484:Zfp341	UTSW	2	154,466,921 (GRCm39)	missense	probably benign	0.00
R5864:Zfp341	UTSW	2	154,485,474 (GRCm39)	missense	possibly damaging	0.95
R5877:Zfp341	UTSW	2	154,474,209 (GRCm39)	missense	probably damaging	1.00
R5975:Zfp341	UTSW	2	154,472,361 (GRCm39)	missense	probably damaging	1.00
R5990:Zfp341	UTSW	2	154,487,579 (GRCm39)	missense	probably damaging	0.98
R6057:Zfp341	UTSW	2	154,466,954 (GRCm39)	missense	probably benign	0.01
R6882:Zfp341	UTSW	2	154,479,943 (GRCm39)	missense	probably damaging	1.00
R7686:Zfp341	UTSW	2	154,466,781 (GRCm39)	missense	probably damaging	0.96
R7701:Zfp341	UTSW	2	154,476,000 (GRCm39)	splice site	probably null
R7847:Zfp341	UTSW	2	154,476,114 (GRCm39)	missense	probably damaging	1.00
R8109:Zfp341	UTSW	2	154,469,820 (GRCm39)	missense	probably benign	0.04
R9313:Zfp341	UTSW	2	154,469,907 (GRCm39)	missense	probably damaging	1.00
R9484:Zfp341	UTSW	2	154,485,763 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCCTCTTCAACCAGATGACGAG -3'
(R):5'- TACATTGCTGCCACCTGGCAAGAC -3'

Sequencing Primer
(F):5'- TTCATGACCCACAAGCGT -3'
(R):5'- aactcttgattctcctgctcc -3'

Posted On

2013-04-11