Mutagenetix > Incidental Mutation

Incidental Mutation 'R1784:Hectd4'

196020

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

039815-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.934) question?

Stock #

R1784 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

121220219-121368577 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 121301839 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 1134 (Y1134F)

Ref Sequence

ENSEMBL: ENSMUSP00000048345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614] [ENSMUST00000100769]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042614
AA Change: Y1134F

PolyPhen 2 Score 0.514 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: Y1134F

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000100769

SMART Domains

Protein: ENSMUSP00000098332
Gene: ENSMUSG00000042744

Domain	Start	End	E-Value	Type
coiled coil region	35	61	N/A	INTRINSIC
low complexity region	214	225	N/A	INTRINSIC
low complexity region	388	404	N/A	INTRINSIC
low complexity region	555	567	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128514

Predicted Effect

probably benign
Transcript: ENSMUST00000148368

Predicted Effect

unknown
Transcript: ENSMUST00000201669
AA Change: Y494F

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.5%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 193 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	T	C	16: 64,769,022 (GRCm38)	I90V	probably damaging	Het
Acacb	TGGGG	TGGG	5: 114,209,767 (GRCm38)		probably null	Het
Adamts17	C	T	7: 67,149,956 (GRCm38)	R1060*	probably null	Het
Adamts5	T	A	16: 85,877,915 (GRCm38)	K454*	probably null	Het
Adgrg6	T	C	10: 14,439,782 (GRCm38)	T593A	probably damaging	Het
Aldh4a1	A	G	4: 139,644,161 (GRCm38)	Y462C	probably damaging	Het
Ankrd12	G	T	17: 65,984,076 (GRCm38)	P1454Q	probably benign	Het
Ap1m1	T	A	8: 72,252,849 (GRCm38)	S230T	probably benign	Het
Aspm	A	G	1: 139,473,574 (GRCm38)	I1111V	probably benign	Het
Atp6ap1l	T	A	13: 90,905,281 (GRCm38)	K4N	probably damaging	Het
Boc	T	C	16: 44,496,419 (GRCm38)	T454A	probably benign	Het
Bola1	C	T	3: 96,197,110 (GRCm38)	G56D	probably benign	Het
C4bp	C	G	1: 130,642,988 (GRCm38)	V284L	probably benign	Het
Cacna1s	T	C	1: 136,118,716 (GRCm38)	F1761S	probably benign	Het
Camsap2	C	T	1: 136,281,315 (GRCm38)	R802Q	probably benign	Het
Capn9	G	A	8: 124,605,711 (GRCm38)	G430R	possibly damaging	Het
Cbs	G	T	17: 31,620,949 (GRCm38)	A337E	probably benign	Het
Ccdc129	T	C	6: 55,968,541 (GRCm38)	F749S	probably benign	Het
Ccdc186	A	C	19: 56,809,220 (GRCm38)	H306Q	probably benign	Het
Cd180	T	C	13: 102,705,859 (GRCm38)	L471P	probably damaging	Het
Cd55	C	T	1: 130,449,423 (GRCm38)	V333I	probably benign	Het
Cd55	C	A	1: 130,459,633 (GRCm38)	A143S	probably benign	Het
Cdk12	T	C	11: 98,249,970 (GRCm38)		probably benign	Het
Cfh	C	T	1: 140,147,697 (GRCm38)	V268I	possibly damaging	Het
Cfhr2	A	C	1: 139,813,459 (GRCm38)	N259K	probably benign	Het
Cfhr2	A	G	1: 139,813,442 (GRCm38)	M265T	probably benign	Het
Chil1	C	T	1: 134,188,529 (GRCm38)	A250V	probably damaging	Het
Chit1	A	G	1: 134,149,394 (GRCm38)	R312G	possibly damaging	Het
Chrna6	A	T	8: 27,406,784 (GRCm38)	M355K	possibly damaging	Het
Clcn1	T	A	6: 42,299,514 (GRCm38)	F360Y	possibly damaging	Het
Copa	T	A	1: 172,111,987 (GRCm38)	F597Y	probably benign	Het
Crb1	A	T	1: 139,237,622 (GRCm38)	H921Q	probably benign	Het
Crb1	G	A	1: 139,241,138 (GRCm38)	P881S	probably damaging	Het
Crb1	C	T	1: 139,242,995 (GRCm38)	G825R	probably damaging	Het
Crb1	C	T	1: 139,243,417 (GRCm38)	R684H	probably benign	Het
Crb1	T	C	1: 139,234,779 (GRCm38)	M1214V	probably benign	Het
Crybg1	T	A	10: 44,004,019 (GRCm38)	Q391L	probably damaging	Het
Cwh43	T	C	5: 73,408,218 (GRCm38)	L42P	probably damaging	Het
Cxcr4	C	T	1: 128,589,277 (GRCm38)	V216I	probably benign	Het
Cyb5r1	C	T	1: 134,407,667 (GRCm38)	R147W	probably damaging	Het
Ddx59	T	C	1: 136,417,053 (GRCm38)	V154A	probably benign	Het
Dnah17	C	T	11: 118,069,519 (GRCm38)	C2572Y	possibly damaging	Het
Dnah9	G	A	11: 66,085,020 (GRCm38)	T1401I	possibly damaging	Het
Dstyk	C	T	1: 132,456,984 (GRCm38)	L739F	probably damaging	Het
Elf2	A	T	3: 51,257,572 (GRCm38)	V277D	probably damaging	Het
Etnk2	C	T	1: 133,363,890 (GRCm38)	P43S	probably benign	Het
Etnk2	T	A	1: 133,376,915 (GRCm38)	V292E	probably benign	Het
Etnk2	G	T	1: 133,377,046 (GRCm38)	A336S	probably benign	Het
Etnk2	C	A	1: 133,365,587 (GRCm38)	D89E	probably benign	Het
Etnk2	C	T	1: 133,365,816 (GRCm38)	R166*	probably null	Het
Etnk2	G	T	1: 133,365,765 (GRCm38)	G149W	probably damaging	Het
Etnk2	G	A	1: 133,365,817 (GRCm38)	R166Q	probably benign	Het
Fam72a	T	C	1: 131,530,668 (GRCm38)	I56T	probably benign	Het
Fam72a	C	T	1: 131,538,895 (GRCm38)	T139M	probably benign	Het
Fat3	A	C	9: 15,996,315 (GRCm38)	V2797G	possibly damaging	Het
Fbxl6	C	T	15: 76,538,058 (GRCm38)	R137H	probably damaging	Het
Fcamr	G	A	1: 130,812,629 (GRCm38)	G262S	probably benign	Het
Fcamr	T	C	1: 130,812,738 (GRCm38)	V298A	probably benign	Het
Fcamr	A	G	1: 130,812,809 (GRCm38)	M322V	probably benign	Het
Fcamr	A	C	1: 130,804,627 (GRCm38)	N117T	probably benign	Het
Fcamr	C	T	1: 130,812,816 (GRCm38)	P324L	probably benign	Het
Fcamr	A	G	1: 130,812,692 (GRCm38)	I283V	probably benign	Het
Fcamr	A	G	1: 130,814,597 (GRCm38)	N574D	probably benign	Het
Fcamr	A	G	1: 130,811,580 (GRCm38)	I206V	probably benign	Het
Fcmr	T	C	1: 130,878,269 (GRCm38)	S321P	probably benign	Het
Fcmr	A	G	1: 130,875,974 (GRCm38)	T172A	probably benign	Het
Gabarap	C	T	11: 69,991,689 (GRCm38)		probably benign	Het
Galnt17	A	T	5: 131,150,963 (GRCm38)	H115Q	probably benign	Het
Gemin4	G	C	11: 76,211,050 (GRCm38)	P962A	probably damaging	Het
Glrx2	C	T	1: 143,739,740 (GRCm38)	A27V	possibly damaging	Het
Gm10563	C	T	4: 155,635,880 (GRCm38)		probably benign	Het
Gm12887	T	A	4: 121,616,518 (GRCm38)	D45V	probably benign	Het
Gse1	G	A	8: 120,568,253 (GRCm38)		probably benign	Het
Guk1	A	T	11: 59,185,312 (GRCm38)	V100E	probably damaging	Het
Heatr4	T	C	12: 83,967,572 (GRCm38)	I630M	probably benign	Het
Ift20	G	A	11: 78,540,034 (GRCm38)	E68K	probably damaging	Het
Igfn1	G	A	1: 135,968,199 (GRCm38)	A1543V	probably benign	Het
Igfn1	C	T	1: 135,972,127 (GRCm38)	R482Q	probably benign	Het
Igfn1	T	C	1: 135,998,625 (GRCm38)	E29G	probably benign	Het
Igfn1	T	C	1: 135,998,683 (GRCm38)	I10V	unknown	Het
Igfn1	G	A	1: 135,982,475 (GRCm38)	R124W	probably benign	Het
Igfn1	C	T	1: 135,979,915 (GRCm38)	A231T	probably benign	Het
Igfn1	G	A	1: 135,959,928 (GRCm38)	P2466L	probably damaging	Het
Igfn1	T	C	1: 135,970,411 (GRCm38)	S806G	probably benign	Het
Ikbke	T	C	1: 131,269,823 (GRCm38)	S447G	probably benign	Het
Ikbke	C	A	1: 131,265,937 (GRCm38)	A459S	probably benign	Het
Ipo9	ATCCTCCTCCTCCTCCTC	ATCCTCCTCCTCCTCCTCCTC	1: 135,386,268 (GRCm38)		probably benign	Het
Ipo9	A	G	1: 135,402,250 (GRCm38)	V484A	probably benign	Het
Kcnh5	T	C	12: 75,137,691 (GRCm38)	D86G	probably benign	Het
Kcnt2	G	A	1: 140,354,547 (GRCm38)	S90N	probably benign	Het
Kif14	T	C	1: 136,515,961 (GRCm38)	F1291L	probably benign	Het
Kif14	C	T	1: 136,503,431 (GRCm38)	L1189F	probably benign	Het
Kif14	A	G	1: 136,468,975 (GRCm38)	K340E	probably damaging	Het
Kif14	G	A	1: 136,478,365 (GRCm38)	A556T	probably benign	Het
Kif14	A	G	1: 136,468,279 (GRCm38)	N108D	probably benign	Het
Kif14	T	C	1: 136,525,783 (GRCm38)	V1433A	probably benign	Het
Kif14	A	G	1: 136,490,332 (GRCm38)	S868G	probably benign	Het
Kif18b	A	G	11: 102,915,541 (GRCm38)		probably null	Het
Kif21b	A	G	1: 136,160,121 (GRCm38)	I983V	possibly damaging	Het
Kpna3	T	A	14: 61,367,701 (GRCm38)	E499V	probably benign	Het
Kremen1	GGG	GGGTGG	11: 5,201,792 (GRCm38)		probably benign	Het
Krt23	A	T	11: 99,492,964 (GRCm38)	V34D	probably damaging	Het
Lad1	C	T	1: 135,828,023 (GRCm38)	R346C	probably damaging	Het
Lad1	C	T	1: 135,827,381 (GRCm38)	P132S	possibly damaging	Het
Lax1	T	C	1: 133,680,569 (GRCm38)	N145D	probably benign	Het
Lax1	T	C	1: 133,679,978 (GRCm38)	R342G	probably benign	Het
Lax1	G	A	1: 133,683,634 (GRCm38)	P67S	probably damaging	Het
Lgr6	C	T	1: 135,003,476 (GRCm38)	S3N	probably benign	Het
Lgr6	A	T	1: 134,988,009 (GRCm38)	S334T	probably benign	Het
Lgr6	C	T	1: 134,987,088 (GRCm38)	V641I	probably benign	Het
Lgr6	G	T	1: 134,990,635 (GRCm38)	H263N	probably benign	Het
Lima1	G	T	15: 99,780,463 (GRCm38)	P539Q	possibly damaging	Het
Lmod1	C	T	1: 135,364,073 (GRCm38)	T222I	probably benign	Het
Megf10	T	C	18: 57,240,792 (GRCm38)		probably null	Het
Mrgbp	T	A	2: 180,585,449 (GRCm38)	N192K	probably damaging	Het
Mrgpra2b	A	G	7: 47,464,879 (GRCm38)	I35T	probably benign	Het
Mroh3	G	C	1: 136,192,144 (GRCm38)	Q440E	possibly damaging	Het
Mrpl3	C	T	9: 105,057,067 (GRCm38)	H130Y	probably benign	Het
Mybph	C	T	1: 134,197,480 (GRCm38)	R249C	probably benign	Het
Mycbp2	A	T	14: 103,155,178 (GRCm38)	C3206S	probably damaging	Het
Nav1	A	T	1: 135,584,727 (GRCm38)	D198E	possibly damaging	Het
Nbeal2	T	A	9: 110,630,857 (GRCm38)	K1844*	probably null	Het
Ndufaf7	A	T	17: 78,937,629 (GRCm38)	K59M	probably damaging	Het
Nr5a2	C	A	1: 136,952,125 (GRCm38)	R35L	probably benign	Het
Obsl1	G	A	1: 75,486,756 (GRCm38)	T1764M	probably benign	Het
Olfr453	C	A	6: 42,744,135 (GRCm38)	L33M	possibly damaging	Het
Olfr870	A	T	9: 20,170,913 (GRCm38)	Y219*	probably null	Het
Optc	A	T	1: 133,903,796 (GRCm38)		probably null	Het
Optc	C	G	1: 133,905,170 (GRCm38)	S64T	probably benign	Het
Otoa	T	C	7: 121,125,439 (GRCm38)	V447A	probably benign	Het
Papss1	A	G	3: 131,605,967 (GRCm38)	N319D	probably benign	Het
Pcdhb3	G	A	18: 37,301,878 (GRCm38)	G299D	probably damaging	Het
Pcsk4	T	C	10: 80,323,570 (GRCm38)	D432G	probably damaging	Het
Pde4b	T	C	4: 102,605,260 (GRCm38)	I711T	probably benign	Het
Pde5a	G	T	3: 122,748,240 (GRCm38)	L126F	probably damaging	Het
Pigr	C	T	1: 130,844,522 (GRCm38)	A159V	possibly damaging	Het
Pik3c2b	C	T	1: 133,066,627 (GRCm38)	P110S	probably benign	Het
Pinx1	A	G	14: 63,878,110 (GRCm38)		probably null	Het
Plekha6	C	G	1: 133,287,846 (GRCm38)	T792S	probably benign	Het
Ppfia4	G	A	1: 134,299,321 (GRCm38)	P1159S	probably benign	Het
Ppil2	C	G	16: 17,089,419 (GRCm38)		probably benign	Het
Prelp	C	T	1: 133,915,131 (GRCm38)	R92K	probably benign	Het
Prrx1	T	A	1: 163,261,967 (GRCm38)	N97I	probably damaging	Het
Ptpn7	A	G	1: 135,134,475 (GRCm38)	Q53R	probably benign	Het
Ptprc	A	G	1: 138,107,823 (GRCm38)	S405P	probably benign	Het
Ptprc	T	C	1: 138,112,254 (GRCm38)	K212E	possibly damaging	Het
Ptprc	T	G	1: 138,099,676 (GRCm38)	N478T	probably benign	Het
Ptprc	A	G	1: 138,107,837 (GRCm38)	V400A	probably benign	Het
Ptprc	C	A	1: 138,107,824 (GRCm38)	E402D	probably benign	Het
Rab29	A	G	1: 131,872,110 (GRCm38)	Q141R	probably benign	Het
Rbsn	A	T	6: 92,190,019 (GRCm38)	L548Q	possibly damaging	Het
Ren1	T	A	1: 133,354,206 (GRCm38)	W22R	probably damaging	Het
Ren1	A	T	1: 133,359,079 (GRCm38)	E315D	probably benign	Het
Ren1	A	T	1: 133,359,983 (GRCm38)	N352Y	probably benign	Het
Ren1	C	T	1: 133,354,237 (GRCm38)	T32I	probably benign	Het
Ren1	C	G	1: 133,350,778 (GRCm38)		probably null	Het
Ren1	A	C	1: 133,356,457 (GRCm38)	K187Q	probably benign	Het
Ren1	C	G	1: 133,360,007 (GRCm38)	L360V	probably benign	Het
Rgs22	T	A	15: 36,087,436 (GRCm38)	K445N	probably damaging	Het
Rint1	T	A	5: 23,809,843 (GRCm38)	D352E	probably benign	Het
Rnpep	C	T	1: 135,263,096 (GRCm38)	A571T	possibly damaging	Het
Sept4	A	T	11: 87,583,436 (GRCm38)	Q60L	probably benign	Het
Sis	A	T	3: 72,965,645 (GRCm38)	C53*	probably null	Het
Slain2	A	G	5: 72,957,614 (GRCm38)	H396R	probably damaging	Het
Slc22a5	G	A	11: 53,866,351 (GRCm38)	P491L	probably damaging	Het
Slc26a9	C	A	1: 131,766,012 (GRCm38)	R747S	probably benign	Het
Slc26a9	C	T	1: 131,763,870 (GRCm38)	A617V	probably benign	Het
Stab2	T	G	10: 86,938,039 (GRCm38)	R809S	probably benign	Het
Tbc1d19	T	G	5: 53,829,372 (GRCm38)	I41S	probably damaging	Het
Tbc1d31	T	A	15: 57,963,920 (GRCm38)	H919Q	possibly damaging	Het
Thsd7b	G	C	1: 129,678,183 (GRCm38)	A554P	probably benign	Het
Thsd7b	C	T	1: 129,628,891 (GRCm38)	T328I	probably damaging	Het
Thsd7b	T	A	1: 129,667,937 (GRCm38)	F498Y	probably benign	Het
Thsd7b	A	C	1: 130,116,631 (GRCm38)	Q1116P	probably benign	Het
Tnfrsf25	T	A	4: 152,118,304 (GRCm38)		probably null	Het
Tnnt2	C	T	1: 135,845,506 (GRCm38)		probably benign	Het
Traf7	A	G	17: 24,512,379 (GRCm38)	F228L	probably damaging	Het
Trim32	T	A	4: 65,614,397 (GRCm38)	I397N	probably damaging	Het
Trove2	T	C	1: 143,760,034 (GRCm38)	D458G	probably benign	Het
Trove2	C	T	1: 143,760,014 (GRCm38)	V465I	probably benign	Het
Tubgcp2	T	C	7: 139,998,055 (GRCm38)	T779A	probably benign	Het
Ube2t	C	T	1: 134,972,167 (GRCm38)	A149V	probably benign	Het
Upf2	A	T	2: 6,027,450 (GRCm38)	S191C	probably damaging	Het
Usp42	T	C	5: 143,714,626 (GRCm38)	D1214G	probably damaging	Het
Vcam1	T	A	3: 116,114,515 (GRCm38)	I633L	probably benign	Het
Vmn2r73	T	C	7: 85,857,878 (GRCm38)	Y742C	probably damaging	Het
Vmn2r81	C	A	10: 79,270,655 (GRCm38)	T489K	probably benign	Het
Zc3h11a	C	T	1: 133,624,621 (GRCm38)	V583I	probably benign	Het
Zc3h11a	G	A	1: 133,622,154 (GRCm38)	P695S	probably benign	Het
Zfp169	C	T	13: 48,489,819 (GRCm38)	A611T	possibly damaging	Het
Zfp281	GCGGCAGCTCCGGCAGC	GCGGCAGCTCCGGCAGCTCCGGCAGC	1: 136,625,353 (GRCm38)		probably benign	Het
Zp3r	A	G	1: 130,596,814 (GRCm38)	L164P	probably benign	Het
Zp3r	C	A	1: 130,619,414 (GRCm38)	E8D	possibly damaging	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,363,870 (GRCm38)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,349,106 (GRCm38)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,331,701 (GRCm38)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,306,950 (GRCm38)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,339,417 (GRCm38)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,336,649 (GRCm38)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,318,651 (GRCm38)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,364,660 (GRCm38)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,322,700 (GRCm38)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,344,824 (GRCm38)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,328,418 (GRCm38)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,366,606 (GRCm38)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,292,087 (GRCm38)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,318,613 (GRCm38)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,344,785 (GRCm38)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,353,881 (GRCm38)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,349,402 (GRCm38)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,342,719 (GRCm38)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,307,004 (GRCm38)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,348,794 (GRCm38)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,259,879 (GRCm38)	missense	probably benign
IGL03181:Hectd4	APN	5	121,353,958 (GRCm38)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,259,939 (GRCm38)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,328,382 (GRCm38)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,307,381 (GRCm38)	nonsense	probably null
agamemnon	UTSW	5	121,253,858 (GRCm38)	splice site	probably benign
clymnestra	UTSW	5	121,334,375 (GRCm38)	missense	possibly damaging	0.86
hector	UTSW	5	121,315,437 (GRCm38)	missense	probably damaging	1.00
helen	UTSW	5	121,310,663 (GRCm38)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,353,551 (GRCm38)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,333,060 (GRCm38)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,254,179 (GRCm38)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,308,576 (GRCm38)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,262,588 (GRCm38)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,349,372 (GRCm38)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,295,506 (GRCm38)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,349,243 (GRCm38)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,329,605 (GRCm38)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,254,251 (GRCm38)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,259,864 (GRCm38)	missense	probably benign
R0410:Hectd4	UTSW	5	121,286,266 (GRCm38)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,343,082 (GRCm38)	splice site	probably null
R0442:Hectd4	UTSW	5	121,323,982 (GRCm38)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,364,590 (GRCm38)	splice site	probably null
R0469:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,295,506 (GRCm38)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,331,707 (GRCm38)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,348,476 (GRCm38)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,304,337 (GRCm38)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,343,232 (GRCm38)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,348,625 (GRCm38)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,277,824 (GRCm38)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,286,463 (GRCm38)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,336,628 (GRCm38)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,307,033 (GRCm38)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,286,769 (GRCm38)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,286,736 (GRCm38)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,310,599 (GRCm38)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,350,485 (GRCm38)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,321,507 (GRCm38)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,318,624 (GRCm38)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,353,695 (GRCm38)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,328,513 (GRCm38)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,349,172 (GRCm38)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,349,172 (GRCm38)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,323,956 (GRCm38)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,349,259 (GRCm38)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,301,878 (GRCm38)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,317,245 (GRCm38)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,298,104 (GRCm38)	missense	probably benign
R1715:Hectd4	UTSW	5	121,344,818 (GRCm38)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,301,839 (GRCm38)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,349,530 (GRCm38)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,358,303 (GRCm38)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,291,191 (GRCm38)	splice site	probably benign
R1843:Hectd4	UTSW	5	121,297,180 (GRCm38)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,281,918 (GRCm38)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,355,629 (GRCm38)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,333,424 (GRCm38)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,318,639 (GRCm38)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,253,858 (GRCm38)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,315,143 (GRCm38)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,353,537 (GRCm38)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,315,437 (GRCm38)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,320,026 (GRCm38)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,263,967 (GRCm38)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,220,620 (GRCm38)	missense	unknown
R2904:Hectd4	UTSW	5	121,292,724 (GRCm38)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,259,873 (GRCm38)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,320,101 (GRCm38)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,303,525 (GRCm38)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,277,834 (GRCm38)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,220,504 (GRCm38)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,350,481 (GRCm38)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,308,271 (GRCm38)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,286,257 (GRCm38)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,314,907 (GRCm38)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,350,431 (GRCm38)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,286,419 (GRCm38)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,297,203 (GRCm38)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,349,216 (GRCm38)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,349,055 (GRCm38)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,325,251 (GRCm38)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,303,615 (GRCm38)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,341,977 (GRCm38)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,348,442 (GRCm38)	missense	probably benign
R4781:Hectd4	UTSW	5	121,306,107 (GRCm38)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,305,818 (GRCm38)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,305,818 (GRCm38)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,322,672 (GRCm38)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,263,891 (GRCm38)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,359,315 (GRCm38)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,322,690 (GRCm38)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,329,565 (GRCm38)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,328,199 (GRCm38)	splice site	probably null
R5129:Hectd4	UTSW	5	121,343,510 (GRCm38)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,353,551 (GRCm38)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,344,824 (GRCm38)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,343,676 (GRCm38)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,263,974 (GRCm38)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,304,448 (GRCm38)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,315,401 (GRCm38)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,310,603 (GRCm38)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,266,274 (GRCm38)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,306,948 (GRCm38)	missense	probably benign
R5507:Hectd4	UTSW	5	121,281,101 (GRCm38)	missense	unknown
R5552:Hectd4	UTSW	5	121,342,851 (GRCm38)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,348,815 (GRCm38)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,353,502 (GRCm38)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,348,619 (GRCm38)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,307,524 (GRCm38)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,343,225 (GRCm38)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,323,974 (GRCm38)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,308,271 (GRCm38)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,308,878 (GRCm38)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,339,498 (GRCm38)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,254,220 (GRCm38)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,350,445 (GRCm38)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,334,375 (GRCm38)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,350,592 (GRCm38)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,356,196 (GRCm38)	splice site	probably null
R6540:Hectd4	UTSW	5	121,303,571 (GRCm38)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,320,084 (GRCm38)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,307,381 (GRCm38)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,277,725 (GRCm38)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,353,511 (GRCm38)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,364,568 (GRCm38)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,299,597 (GRCm38)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,273,629 (GRCm38)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,308,342 (GRCm38)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,329,073 (GRCm38)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,314,881 (GRCm38)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,310,663 (GRCm38)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,281,932 (GRCm38)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,323,961 (GRCm38)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,358,133 (GRCm38)	splice site	probably null
R7482:Hectd4	UTSW	5	121,363,878 (GRCm38)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,297,109 (GRCm38)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,343,665 (GRCm38)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,315,510 (GRCm38)	splice site	probably null
R7560:Hectd4	UTSW	5	121,254,342 (GRCm38)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,291,225 (GRCm38)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,349,459 (GRCm38)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,318,735 (GRCm38)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,254,371 (GRCm38)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,324,031 (GRCm38)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,321,564 (GRCm38)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,220,617 (GRCm38)	missense	unknown
R7731:Hectd4	UTSW	5	121,307,014 (GRCm38)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,336,629 (GRCm38)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,305,721 (GRCm38)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,329,568 (GRCm38)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,331,817 (GRCm38)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,254,228 (GRCm38)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,310,629 (GRCm38)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,339,518 (GRCm38)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,321,398 (GRCm38)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,332,949 (GRCm38)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,286,376 (GRCm38)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,318,756 (GRCm38)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,339,544 (GRCm38)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,266,361 (GRCm38)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,317,225 (GRCm38)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,220,256 (GRCm38)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,259,894 (GRCm38)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,308,358 (GRCm38)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,343,147 (GRCm38)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,329,109 (GRCm38)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,220,256 (GRCm38)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,349,010 (GRCm38)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,304,426 (GRCm38)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,353,598 (GRCm38)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,310,651 (GRCm38)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,350,494 (GRCm38)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,363,775 (GRCm38)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,281,873 (GRCm38)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,323,931 (GRCm38)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,295,478 (GRCm38)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,328,242 (GRCm38)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,277,756 (GRCm38)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,358,284 (GRCm38)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,303,566 (GRCm38)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,254,359 (GRCm38)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,313,892 (GRCm38)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,273,614 (GRCm38)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,329,556 (GRCm38)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,358,175 (GRCm38)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,349,034 (GRCm38)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,253,904 (GRCm38)	missense
R9162:Hectd4	UTSW	5	121,306,979 (GRCm38)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,308,627 (GRCm38)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,299,488 (GRCm38)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,295,433 (GRCm38)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,348,965 (GRCm38)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,348,889 (GRCm38)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,299,645 (GRCm38)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,334,429 (GRCm38)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,322,801 (GRCm38)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,314,918 (GRCm38)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,364,553 (GRCm38)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,321,554 (GRCm38)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,334,469 (GRCm38)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,286,781 (GRCm38)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,254,191 (GRCm38)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,334,352 (GRCm38)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,349,637 (GRCm38)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,321,404 (GRCm38)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,295,503 (GRCm38)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,358,320 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GATCTTAGTGTCCCTGTGCCAACC -3'
(R):5'- CCATACCAATGTGGCCCTAACTAGC -3'

Sequencing Primer
(F):5'- TACCAGGGTCTGGCTATAAATG -3'
(R):5'- GTGGCCCTAACTAGCTAACTATGTG -3'

Posted On

2014-05-23