Mutagenetix > Incidental Mutation

Incidental Mutation 'R0085:Wdfy4'

19868

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

038372-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0085 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32959547-33185508 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 33078243 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 1975 (R1975S)

Ref Sequence

ENSEMBL: ENSMUSP00000117068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000061753
AA Change: R1821S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: R1821S

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130509
AA Change: R1975S

PolyPhen 2 Score 0.809 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: R1975S

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Meta Mutation Damage Score

0.1537

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.4%

Validation Efficiency

95% (71/75)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad12	A	G	5: 121,604,294 (GRCm38)	I417T	possibly damaging	Het
Adcy9	T	C	16: 4,288,224 (GRCm38)	T1009A	probably benign	Het
Ass1	A	T	2: 31,514,819 (GRCm38)	N371Y	probably damaging	Het
Baat	T	C	4: 49,490,425 (GRCm38)		probably benign	Het
Bpi	T	A	2: 158,273,152 (GRCm38)	L311*	probably null	Het
Brd2	A	C	17: 34,113,259 (GRCm38)	F294L	probably damaging	Het
Carmil1	T	A	13: 24,025,867 (GRCm38)	E804D	probably benign	Het
Cd209g	A	T	8: 4,134,785 (GRCm38)		probably benign	Het
Cfi	A	G	3: 129,874,986 (GRCm38)	I554V	probably benign	Het
Clvs2	G	C	10: 33,622,546 (GRCm38)	S129R	possibly damaging	Het
Dst	T	C	1: 34,229,187 (GRCm38)	S2897P	probably damaging	Het
Efcab7	T	C	4: 99,904,680 (GRCm38)		probably benign	Het
Fbxo2	T	C	4: 148,164,910 (GRCm38)		probably null	Het
Fgfr2	C	A	7: 130,196,263 (GRCm38)	R400L	probably damaging	Het
Hsd17b14	T	C	7: 45,556,410 (GRCm38)		probably benign	Het
Il23r	T	C	6: 67,486,222 (GRCm38)	N96D	probably damaging	Het
Ints13	T	A	6: 146,574,787 (GRCm38)		probably benign	Het
Khdc4	T	A	3: 88,711,739 (GRCm38)	S583R	probably damaging	Het
Lig1	A	G	7: 13,307,570 (GRCm38)	I776V	possibly damaging	Het
Madd	T	C	2: 91,162,738 (GRCm38)	I997V	probably benign	Het
Mgat4b	C	T	11: 50,230,999 (GRCm38)	H116Y	possibly damaging	Het
Myh11	C	A	16: 14,224,019 (GRCm38)	Q720H	probably damaging	Het
Myo5b	A	C	18: 74,701,680 (GRCm38)	D937A	probably benign	Het
Nox3	T	C	17: 3,635,281 (GRCm38)	N584S	probably benign	Het
Ogfr	A	G	2: 180,591,037 (GRCm38)		probably null	Het
Or11g25	T	A	14: 50,486,334 (GRCm38)	M292K	probably benign	Het
Or13p3	T	C	4: 118,709,881 (GRCm38)	V158A	probably benign	Het
Or8b1b	T	C	9: 38,464,662 (GRCm38)	I207T	probably benign	Het
Osbpl6	G	T	2: 76,593,414 (GRCm38)	V728F	probably benign	Het
Picalm	T	A	7: 90,182,317 (GRCm38)	S453T	probably benign	Het
Piezo1	A	G	8: 122,501,615 (GRCm38)	L310P	probably damaging	Het
Pitrm1	C	T	13: 6,549,568 (GRCm38)		probably benign	Het
Pkd1	T	C	17: 24,586,223 (GRCm38)	F3250L	probably damaging	Het
Plekha4	C	T	7: 45,543,949 (GRCm38)	R376*	probably null	Het
Pnma8b	A	T	7: 16,945,549 (GRCm38)	S153C	unknown	Het
Rp1l1	C	T	14: 64,022,295 (GRCm38)	R129W	probably damaging	Het
Ryr3	A	G	2: 112,859,763 (GRCm38)	V1147A	probably damaging	Het
Sema3d	G	A	5: 12,570,986 (GRCm38)	V520I	probably benign	Het
Sgsm1	A	G	5: 113,279,270 (GRCm38)		probably benign	Het
Slc13a2	A	G	11: 78,406,868 (GRCm38)	V58A	probably damaging	Het
Slc1a4	A	G	11: 20,304,510 (GRCm38)		probably benign	Het
Slc4a10	G	A	2: 62,244,346 (GRCm38)		probably benign	Het
Tab1	G	T	15: 80,155,893 (GRCm38)	A305S	probably benign	Het
Tmem30a	T	A	9: 79,771,294 (GRCm38)	T327S	probably benign	Het
Tpr	A	C	1: 150,417,413 (GRCm38)	E863A	possibly damaging	Het
Upk3bl	A	G	5: 136,060,115 (GRCm38)	N161D	probably benign	Het
Ush1c	T	A	7: 46,225,555 (GRCm38)	I131F	probably benign	Het
Zbtb18	C	T	1: 177,447,935 (GRCm38)	A287V	probably benign	Het
Zfp712	T	C	13: 67,041,192 (GRCm38)	T424A	probably benign	Het
Zfp791	G	T	8: 85,112,233 (GRCm38)	Y56*	probably null	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	33,102,539 (GRCm38)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,959,977 (GRCm38)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	33,104,037 (GRCm38)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	33,151,661 (GRCm38)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	33,020,238 (GRCm38)	splice site	probably benign
IGL01931:Wdfy4	APN	14	33,155,753 (GRCm38)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	33,133,716 (GRCm38)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	33,076,480 (GRCm38)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	33,093,300 (GRCm38)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	33,149,566 (GRCm38)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,966,432 (GRCm38)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	33,042,491 (GRCm38)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	33,090,861 (GRCm38)	nonsense	probably null
IGL02752:Wdfy4	APN	14	33,076,326 (GRCm38)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	33,095,305 (GRCm38)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,971,750 (GRCm38)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	33,109,650 (GRCm38)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	33,076,284 (GRCm38)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	33,140,651 (GRCm38)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,966,435 (GRCm38)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	33,162,870 (GRCm38)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	33,125,887 (GRCm38)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,977,167 (GRCm38)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	33,068,904 (GRCm38)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	33,047,290 (GRCm38)	missense	probably benign	0.14
dodgers	UTSW	14	32,977,106 (GRCm38)	nonsense	probably null
Dollar	UTSW	14	33,020,311 (GRCm38)	missense	probably damaging	1.00
Giants	UTSW	14	33,070,618 (GRCm38)	nonsense	probably null
gigantea	UTSW	14	32,974,154 (GRCm38)	critical splice donor site	probably null
kings_canyon	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
moro	UTSW	14	32,964,626 (GRCm38)	splice site	probably null
popped	UTSW	14	32,966,399 (GRCm38)	missense	probably damaging	0.99
sequoia	UTSW	14	33,100,903 (GRCm38)	critical splice donor site	probably null
Sherman	UTSW	14	33,095,951 (GRCm38)	missense	possibly damaging	0.89
stretched	UTSW	14	33,073,535 (GRCm38)	nonsense	probably null
watchtower	UTSW	14	33,083,639 (GRCm38)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	33,107,173 (GRCm38)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	33,162,751 (GRCm38)	missense	probably null	1.00
R0277:Wdfy4	UTSW	14	33,083,785 (GRCm38)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	33,083,812 (GRCm38)	splice site	probably benign
R0496:Wdfy4	UTSW	14	33,140,738 (GRCm38)	splice site	probably benign
R0514:Wdfy4	UTSW	14	33,080,775 (GRCm38)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	33,042,621 (GRCm38)	missense	probably benign
R0590:Wdfy4	UTSW	14	33,041,174 (GRCm38)	missense	probably benign	0.09
R0647:Wdfy4	UTSW	14	33,109,699 (GRCm38)	missense	possibly damaging	0.96
R0766:Wdfy4	UTSW	14	33,140,612 (GRCm38)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	33,147,092 (GRCm38)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	33,079,966 (GRCm38)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,971,738 (GRCm38)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,971,772 (GRCm38)	splice site	probably null
R1415:Wdfy4	UTSW	14	33,041,180 (GRCm38)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	33,108,688 (GRCm38)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	33,100,966 (GRCm38)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	33,152,538 (GRCm38)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,960,808 (GRCm38)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	33,042,512 (GRCm38)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,959,961 (GRCm38)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	33,073,585 (GRCm38)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	33,096,005 (GRCm38)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	33,103,983 (GRCm38)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	33,133,344 (GRCm38)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,971,684 (GRCm38)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	33,106,044 (GRCm38)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	33,146,989 (GRCm38)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	33,073,511 (GRCm38)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	33,162,807 (GRCm38)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
R2889:Wdfy4	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
R3114:Wdfy4	UTSW	14	33,089,903 (GRCm38)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	33,023,374 (GRCm38)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	33,023,374 (GRCm38)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	33,140,645 (GRCm38)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	33,047,280 (GRCm38)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	33,047,280 (GRCm38)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,966,395 (GRCm38)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	33,102,680 (GRCm38)	splice site	probably benign
R4091:Wdfy4	UTSW	14	33,125,880 (GRCm38)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	33,096,083 (GRCm38)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	33,087,955 (GRCm38)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	33,102,558 (GRCm38)	missense	probably damaging	0.97
R4629:Wdfy4	UTSW	14	33,102,558 (GRCm38)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,989,936 (GRCm38)	missense	probably damaging	0.98
R4689:Wdfy4	UTSW	14	33,109,548 (GRCm38)	missense	possibly damaging	0.88
R4718:Wdfy4	UTSW	14	33,145,316 (GRCm38)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	33,100,903 (GRCm38)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,988,895 (GRCm38)	nonsense	probably null
R4894:Wdfy4	UTSW	14	33,155,760 (GRCm38)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	33,047,256 (GRCm38)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	33,029,013 (GRCm38)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	33,100,940 (GRCm38)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	33,079,935 (GRCm38)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	33,152,670 (GRCm38)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	33,047,275 (GRCm38)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	33,078,343 (GRCm38)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	33,090,866 (GRCm38)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,988,899 (GRCm38)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,960,002 (GRCm38)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	33,020,311 (GRCm38)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	33,151,732 (GRCm38)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	33,107,130 (GRCm38)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	33,133,497 (GRCm38)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	33,107,618 (GRCm38)	splice site	probably null
R5890:Wdfy4	UTSW	14	33,102,577 (GRCm38)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	33,133,360 (GRCm38)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	33,106,011 (GRCm38)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	33,146,990 (GRCm38)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	33,146,990 (GRCm38)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	33,083,639 (GRCm38)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,971,711 (GRCm38)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	33,109,525 (GRCm38)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	33,101,049 (GRCm38)	nonsense	probably null
R6370:Wdfy4	UTSW	14	33,068,850 (GRCm38)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	33,104,094 (GRCm38)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,967,647 (GRCm38)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	33,108,692 (GRCm38)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	33,146,944 (GRCm38)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	33,047,251 (GRCm38)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	33,095,951 (GRCm38)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	33,042,512 (GRCm38)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,959,966 (GRCm38)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	33,099,117 (GRCm38)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,964,626 (GRCm38)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,960,820 (GRCm38)	missense
R7114:Wdfy4	UTSW	14	32,971,574 (GRCm38)	splice site	probably null
R7139:Wdfy4	UTSW	14	33,151,578 (GRCm38)	missense
R7255:Wdfy4	UTSW	14	32,974,282 (GRCm38)	missense
R7324:Wdfy4	UTSW	14	33,047,314 (GRCm38)	missense
R7379:Wdfy4	UTSW	14	33,151,609 (GRCm38)	missense
R7399:Wdfy4	UTSW	14	33,068,906 (GRCm38)	missense
R7408:Wdfy4	UTSW	14	33,078,307 (GRCm38)	missense
R7410:Wdfy4	UTSW	14	32,974,234 (GRCm38)	missense
R7411:Wdfy4	UTSW	14	33,106,131 (GRCm38)	missense
R7412:Wdfy4	UTSW	14	33,149,584 (GRCm38)	missense
R7445:Wdfy4	UTSW	14	33,070,618 (GRCm38)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,974,154 (GRCm38)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,985,739 (GRCm38)	missense
R7622:Wdfy4	UTSW	14	33,078,274 (GRCm38)	missense
R7828:Wdfy4	UTSW	14	32,988,921 (GRCm38)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	33,090,963 (GRCm38)	missense
R7946:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R7946:Wdfy4	UTSW	14	33,070,748 (GRCm38)	missense
R7986:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R7990:Wdfy4	UTSW	14	33,097,795 (GRCm38)	missense
R8001:Wdfy4	UTSW	14	32,973,535 (GRCm38)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,971,627 (GRCm38)	missense
R8015:Wdfy4	UTSW	14	33,107,747 (GRCm38)	missense
R8032:Wdfy4	UTSW	14	33,029,086 (GRCm38)	nonsense	probably null
R8041:Wdfy4	UTSW	14	33,154,008 (GRCm38)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8092:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8112:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8114:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8115:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8117:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8117:Wdfy4	UTSW	14	32,977,106 (GRCm38)	nonsense	probably null
R8118:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8140:Wdfy4	UTSW	14	33,142,360 (GRCm38)	missense
R8155:Wdfy4	UTSW	14	33,162,819 (GRCm38)	missense
R8163:Wdfy4	UTSW	14	33,151,588 (GRCm38)	missense
R8293:Wdfy4	UTSW	14	32,974,261 (GRCm38)	missense
R8325:Wdfy4	UTSW	14	32,967,487 (GRCm38)	missense
R8353:Wdfy4	UTSW	14	32,973,624 (GRCm38)	missense	probably benign
R8370:Wdfy4	UTSW	14	33,093,251 (GRCm38)	missense
R8437:Wdfy4	UTSW	14	33,076,375 (GRCm38)	missense
R8497:Wdfy4	UTSW	14	32,966,399 (GRCm38)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	33,078,301 (GRCm38)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,971,765 (GRCm38)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,967,532 (GRCm38)	missense
R8747:Wdfy4	UTSW	14	33,152,654 (GRCm38)	missense
R8794:Wdfy4	UTSW	14	33,147,092 (GRCm38)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	33,145,148 (GRCm38)	missense
R8880:Wdfy4	UTSW	14	33,073,535 (GRCm38)	nonsense	probably null
R9109:Wdfy4	UTSW	14	33,038,747 (GRCm38)	splice site	probably null
R9131:Wdfy4	UTSW	14	33,097,850 (GRCm38)	missense
R9309:Wdfy4	UTSW	14	33,095,356 (GRCm38)	missense
R9349:Wdfy4	UTSW	14	33,154,039 (GRCm38)	missense
R9451:Wdfy4	UTSW	14	33,133,561 (GRCm38)	missense
R9563:Wdfy4	UTSW	14	32,970,876 (GRCm38)	missense
R9587:Wdfy4	UTSW	14	33,047,273 (GRCm38)	nonsense	probably null
R9599:Wdfy4	UTSW	14	33,133,471 (GRCm38)	missense
R9670:Wdfy4	UTSW	14	33,047,262 (GRCm38)	missense
R9718:Wdfy4	UTSW	14	33,125,936 (GRCm38)	missense
R9742:Wdfy4	UTSW	14	33,088,030 (GRCm38)	missense
X0028:Wdfy4	UTSW	14	33,080,636 (GRCm38)	missense	probably benign
X0053:Wdfy4	UTSW	14	33,162,942 (GRCm38)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	33,107,618 (GRCm38)	splice site	probably null
Z1177:Wdfy4	UTSW	14	33,087,985 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- CCATTTGCTGATGGACTGCTGAGAG -3'
(R):5'- CTGTGGGGTGAAGAAATCCACCAG -3'

Sequencing Primer
(F):5'- TAGGGCTCAAGGCTTCATCAC -3'
(R):5'- ATCCACCAGACACTAATTTTGGTC -3'

Posted On

2013-04-11