|Institutional Source||Beutler Lab|
|Gene Name||mitogen-activated protein kinase kinase kinase 2|
|Synonyms||MEK kinase 2, Mekk2, 9630061B06Rik|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R0086 (G1)|
|Chromosomal Location||32163089-32236751 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 32218468 bp|
|Amino Acid Change||Isoleucine to Phenylalanine at position 435 (I435F)|
|Ref Sequence||ENSEMBL: ENSMUSP00000094326 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000096575]|
|Predicted Effect||probably damaging
AA Change: I435F
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: I435F
|Meta Mutation Damage Score||0.6467|
|Coding Region Coverage||
|Validation Efficiency||96% (91/95)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene are grossly normal and fertile. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Map3k2||
(F):5'- AGAGTAGACATTCAGAGGCATACACACA -3'
(R):5'- TAGACGATGATCAGCAGTTCTACGGT -3'
(F):5'- tcagagacatacacacacatagac -3'
(R):5'- CAGCAGTTCTACGGTAGGCAG -3'