Incidental Mutation 'R0086:Map3k2'
ID19919
Institutional Source Beutler Lab
Gene Symbol Map3k2
Ensembl Gene ENSMUSG00000024383
Gene Namemitogen-activated protein kinase kinase kinase 2
SynonymsMEK kinase 2, Mekk2, 9630061B06Rik
MMRRC Submission 038373-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R0086 (G1)
Quality Score225
Status Validated
Chromosome18
Chromosomal Location32163089-32236751 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 32218468 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 435 (I435F)
Ref Sequence ENSEMBL: ENSMUSP00000094326 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000096575]
Predicted Effect probably damaging
Transcript: ENSMUST00000096575
AA Change: I435F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000094326
Gene: ENSMUSG00000024383
AA Change: I435F

DomainStartEndE-ValueType
PB1 43 122 6.96e-20 SMART
low complexity region 203 219 N/A INTRINSIC
low complexity region 300 315 N/A INTRINSIC
low complexity region 322 334 N/A INTRINSIC
S_TKc 356 616 2.86e-92 SMART
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.0%
  • 10x: 94.6%
  • 20x: 86.6%
Validation Efficiency 96% (91/95)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene are grossly normal and fertile. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930407I10Rik T A 15: 82,062,601 V233D probably benign Het
Abcg8 T C 17: 84,692,771 V252A probably damaging Het
Adam39 C T 8: 40,826,360 T596I possibly damaging Het
Agap2 C A 10: 127,087,882 probably null Het
Ap4b1 T G 3: 103,814,860 V50G probably damaging Het
Atp13a1 T A 8: 69,797,774 I381N possibly damaging Het
Bcl2 G A 1: 106,712,562 R107C probably damaging Het
Birc6 T C 17: 74,593,166 V1113A possibly damaging Het
C1galt1 T A 6: 7,867,051 probably benign Het
Capza2 A G 6: 17,660,774 K158E probably damaging Het
Cenpe C T 3: 135,264,424 probably benign Het
Cercam T C 2: 29,871,064 L42P probably damaging Het
Cfap54 T C 10: 93,028,594 E807G possibly damaging Het
Cog6 A G 3: 52,993,570 V157A probably damaging Het
Cts6 A T 13: 61,196,457 probably benign Het
Cyp2c39 A T 19: 39,510,913 I15F unknown Het
Dock7 A T 4: 98,945,144 V1970D probably damaging Het
Exph5 A G 9: 53,337,930 D73G possibly damaging Het
Gjc2 A T 11: 59,176,846 M270K probably benign Het
Gns G A 10: 121,391,473 D463N probably damaging Het
Hoxd8 G T 2: 74,705,932 G129W probably damaging Het
Ina A G 19: 47,023,591 T483A possibly damaging Het
Lmod3 T A 6: 97,247,345 Q505L probably damaging Het
Map3k13 A G 16: 21,914,225 N526D probably damaging Het
Mfsd6l A G 11: 68,556,565 T81A probably benign Het
Micall1 T C 15: 79,125,489 probably benign Het
Mkrn2 G T 6: 115,613,335 M217I possibly damaging Het
Myh11 C A 16: 14,224,019 Q720H probably damaging Het
Ncapg A G 5: 45,676,744 probably null Het
Nlrp9a G A 7: 26,558,547 C530Y probably damaging Het
Numb T C 12: 83,795,930 T442A probably damaging Het
Oip5 C T 2: 119,617,929 probably benign Het
Olfr1224-ps1 C T 2: 89,156,476 R233H probably benign Het
Olfr342 A T 2: 36,527,450 I13F possibly damaging Het
Olfr639 A G 7: 104,012,054 I216T probably benign Het
Olfr936 T C 9: 39,046,895 T175A probably benign Het
Pcnx T C 12: 81,992,058 probably benign Het
Pkhd1l1 T A 15: 44,556,008 N2956K possibly damaging Het
Plcl1 T A 1: 55,715,583 W1030R probably damaging Het
Polr2i G A 7: 30,233,086 V73M probably damaging Het
Prr14l T A 5: 32,831,559 probably benign Het
Pxdn G T 12: 30,002,419 R865L possibly damaging Het
Scnn1a T C 6: 125,342,587 probably benign Het
Shkbp1 G T 7: 27,352,026 H203N probably benign Het
Skiv2l2 G T 13: 112,927,328 F10L probably benign Het
Slc22a14 C T 9: 119,222,738 probably benign Het
Snap29 C A 16: 17,428,236 T240K probably damaging Het
Sp2 C A 11: 96,957,427 G457C probably damaging Het
Ssr2 C T 3: 88,576,880 probably benign Het
Synpo2 A T 3: 123,117,104 C297* probably null Het
Tpm3 T A 3: 90,090,092 probably benign Het
Trmt6 CTG C 2: 132,809,017 probably benign Het
Trp63 T C 16: 25,871,087 Y431H probably damaging Het
Tuba3b T A 6: 145,621,160 C376S probably damaging Het
Ubxn4 G A 1: 128,262,904 E256K probably benign Het
Ulk1 G A 5: 110,787,707 probably benign Het
Usp24 T C 4: 106,392,360 S1425P probably damaging Het
Xdh T C 17: 73,884,438 I1335V probably benign Het
Zmynd15 T C 11: 70,464,232 Y352H probably damaging Het
Other mutations in Map3k2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00770:Map3k2 APN 18 32228239 missense probably benign 0.00
IGL00774:Map3k2 APN 18 32228239 missense probably benign 0.00
IGL01993:Map3k2 APN 18 32226631 nonsense probably null
IGL02043:Map3k2 APN 18 32207534 missense probably damaging 1.00
IGL02314:Map3k2 APN 18 32218500 splice site probably benign
IGL02441:Map3k2 APN 18 32200046 splice site probably benign
IGL03350:Map3k2 APN 18 32212148 missense probably damaging 0.98
IGL03097:Map3k2 UTSW 18 32200017 missense probably benign 0.01
PIT4434001:Map3k2 UTSW 18 32210035 missense possibly damaging 0.51
R0374:Map3k2 UTSW 18 32212173 splice site probably null
R0445:Map3k2 UTSW 18 32217210 missense probably damaging 0.96
R1158:Map3k2 UTSW 18 32217158 missense probably benign 0.00
R1415:Map3k2 UTSW 18 32228277 missense possibly damaging 0.82
R1667:Map3k2 UTSW 18 32203792 splice site probably benign
R1926:Map3k2 UTSW 18 32203110 missense probably damaging 0.99
R3795:Map3k2 UTSW 18 32226648 missense probably benign 0.00
R4607:Map3k2 UTSW 18 32199977 missense probably damaging 1.00
R4793:Map3k2 UTSW 18 32228150 missense probably damaging 1.00
R5332:Map3k2 UTSW 18 32207456 missense probably damaging 0.98
R5492:Map3k2 UTSW 18 32228136 missense probably damaging 1.00
R6008:Map3k2 UTSW 18 32203051 missense probably damaging 1.00
R6317:Map3k2 UTSW 18 32203033 missense probably damaging 1.00
R6356:Map3k2 UTSW 18 32211970 missense probably damaging 1.00
R6841:Map3k2 UTSW 18 32226629 missense probably benign 0.12
R6928:Map3k2 UTSW 18 32207540 critical splice donor site probably null
R7475:Map3k2 UTSW 18 32199962 missense possibly damaging 0.61
R7696:Map3k2 UTSW 18 32220594 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- AGAGTAGACATTCAGAGGCATACACACA -3'
(R):5'- TAGACGATGATCAGCAGTTCTACGGT -3'

Sequencing Primer
(F):5'- tcagagacatacacacacatagac -3'
(R):5'- CAGCAGTTCTACGGTAGGCAG -3'
Posted On2013-04-11