Mutagenetix > Incidental Mutation

Incidental Mutation 'R1732:Gpr25'

199361

Institutional Source

Beutler Lab

Gene Symbol

Gpr25

Ensembl Gene

ENSMUSG00000052759

Gene Name

G protein-coupled receptor 25

Synonyms

LOC383563

MMRRC Submission

039764-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1732 (G1)

Quality Score

116

Status

Validated

Chromosome

Chromosomal Location

136259797-136260873 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 136260128 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 249 (V249A)

Ref Sequence

ENSEMBL: ENSMUSP00000083583 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086395]

AlphaFold

P0C5I1

Predicted Effect

probably benign
Transcript: ENSMUST00000086395
AA Change: V249A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000083583
Gene: ENSMUSG00000052759
AA Change: V249A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	56	304	1.1e-40	PFAM
low complexity region	328	342	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181385

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000193939

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.6%

Validation Efficiency

99% (78/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	C	A	12: 71,219,221	Q1445K	probably benign	Het
Acacb	A	T	5: 114,190,087	M303L	possibly damaging	Het
Actl9	T	C	17: 33,433,122	V52A	probably damaging	Het
Adam7	T	C	14: 68,498,450	T781A	probably benign	Het
Agpat4	T	C	17: 12,216,728	V293A	probably benign	Het
Ank1	T	A	8: 23,111,463		probably benign	Het
Atp1a1	C	A	3: 101,584,799	G587V	probably damaging	Het
Avpr1b	T	C	1: 131,600,254	F172L	probably damaging	Het
Bmp1	A	T	14: 70,486,265	D710E	possibly damaging	Het
Cep126	A	T	9: 8,099,761	I924N	probably benign	Het
Chek2	G	T	5: 110,872,102	A517S	probably benign	Het
Cmbl	A	T	15: 31,588,232	E165D	probably damaging	Het
Cntnap3	A	T	13: 64,740,812		probably null	Het
Col1a1	A	T	11: 94,944,415		probably benign	Het
Ctbp2	T	C	7: 132,998,924	M624V	possibly damaging	Het
Cwc15	A	G	9: 14,510,247	D203G	probably benign	Het
Cyld	C	T	8: 88,731,667		probably benign	Het
Cyp3a57	A	T	5: 145,365,645	I84F	probably damaging	Het
Dennd3	T	A	15: 73,537,418		probably benign	Het
Desi2	A	C	1: 178,256,651		probably benign	Het
Dmxl1	T	A	18: 49,893,444	L1873*	probably null	Het
Dmxl1	T	A	18: 49,902,988	H2359Q	probably benign	Het
Ephb4	A	G	5: 137,372,178	N880S	possibly damaging	Het
F10	T	C	8: 13,050,764	L214P	probably damaging	Het
F5	G	A	1: 164,174,150	V141M	probably damaging	Het
Fam135a	A	T	1: 24,026,653	S1022T	possibly damaging	Het
Fam13b	A	T	18: 34,487,134	N232K	probably benign	Het
Fyco1	T	C	9: 123,819,092	E1259G	probably benign	Het
Gak	A	T	5: 108,576,582	D1087E	probably benign	Het
Gm7138	A	T	10: 77,776,848		probably benign	Het
Gm8674	T	A	13: 49,901,926		noncoding transcript	Het
Hcn3	T	C	3: 89,148,119	H607R	probably damaging	Het
Hdac4	T	A	1: 91,947,535	T905S	probably benign	Het
Itgad	T	C	7: 128,205,107	S86P	probably benign	Het
Itgb4	G	T	11: 115,988,918	R632L	probably damaging	Het
Kcnb2	A	T	1: 15,709,755	T284S	probably benign	Het
Klhl22	T	A	16: 17,777,024	M339K	probably damaging	Het
Lonrf2	G	A	1: 38,813,276	P165S	probably benign	Het
Mybpc2	C	A	7: 44,513,675	V484L	probably benign	Het
Noa1	A	T	5: 77,306,374	V473E	probably benign	Het
Nwd1	C	G	8: 72,666,835	S242C	possibly damaging	Het
Olfr545	C	A	7: 102,494,040	C245F	probably damaging	Het
Olfr868	A	G	9: 20,101,500	H247R	probably damaging	Het
Otof	A	T	5: 30,386,471	W535R	probably damaging	Het
Peg3	T	C	7: 6,709,085	E1046G	possibly damaging	Het
Phldb2	T	C	16: 45,757,166	E1132G	probably damaging	Het
Phtf2	A	T	5: 20,789,627		probably null	Het
Plce1	G	T	19: 38,716,838	A896S	possibly damaging	Het
Prim1	T	C	10: 128,015,324	Y26H	probably damaging	Het
Prr12	T	A	7: 45,048,356	T712S	unknown	Het
Psme4	G	A	11: 30,848,105	R1366H	probably benign	Het
Ptpdc1	T	C	13: 48,586,545	E409G	probably benign	Het
Rabep1	A	G	11: 70,904,641	N274S	probably damaging	Het
Rbm14	A	G	19: 4,803,467	S296P	probably benign	Het
Rec114	A	G	9: 58,653,106	S206P	probably damaging	Het
Rps6ka1	A	T	4: 133,860,070	Y531N	probably damaging	Het
Slc28a2	T	C	2: 122,449,758		probably benign	Het
Slc34a1	A	G	13: 55,413,420	H566R	probably benign	Het
Slc9c1	A	G	16: 45,552,928	T290A	probably benign	Het
Smarcc1	T	A	9: 110,185,820		probably benign	Het
Srp54b	T	A	12: 55,252,759		probably null	Het
Stambpl1	C	G	19: 34,226,721	N70K	probably damaging	Het
Synj1	T	C	16: 90,964,230	K710E	probably damaging	Het
Tenm3	T	A	8: 48,310,634	D795V	probably damaging	Het
Tgm2	T	C	2: 158,134,357	Y149C	probably damaging	Het
Tmem151a	C	A	19: 5,082,867	A104S	probably damaging	Het
Top1mt	A	G	15: 75,666,251		probably null	Het
Tpgs1	T	A	10: 79,675,594	L190Q	possibly damaging	Het
Triobp	T	A	15: 78,967,228	H527Q	possibly damaging	Het
Tspan2	A	G	3: 102,768,877	I197V	probably damaging	Het
Ube3b	A	G	5: 114,387,445	I76V	probably benign	Het
Vmn1r32	A	T	6: 66,553,301	S164T	probably benign	Het
Zfp607a	G	T	7: 27,878,459	C318F	probably damaging	Het

Other mutations in Gpr25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03028:Gpr25	APN	1	136260812	missense	probably benign	0.00
R1728:Gpr25	UTSW	1	136260710	missense	probably benign
R1729:Gpr25	UTSW	1	136260710	missense	probably benign
R1739:Gpr25	UTSW	1	136260710	missense	probably benign
R1762:Gpr25	UTSW	1	136260710	missense	probably benign
R1783:Gpr25	UTSW	1	136260710	missense	probably benign
R1785:Gpr25	UTSW	1	136260710	missense	probably benign
R1907:Gpr25	UTSW	1	136260800	missense	probably benign	0.00
R2877:Gpr25	UTSW	1	136260815	missense	possibly damaging	0.86
R3081:Gpr25	UTSW	1	136259885	missense	possibly damaging	0.68
R5132:Gpr25	UTSW	1	136260365	missense	probably damaging	1.00
R7133:Gpr25	UTSW	1	136260821	missense	probably damaging	1.00
R8163:Gpr25	UTSW	1	136259858	missense	probably damaging	1.00
R8248:Gpr25	UTSW	1	136260677	missense	probably benign	0.03
R9051:Gpr25	UTSW	1	136260288	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGCTATTGACGAAAGCCAGGCAG -3'
(R):5'- TGTGCAAAGTCAGCAGCTTCGC -3'

Sequencing Primer
(F):5'- CAGGTGGTGACAGTCAGACC -3'
(R):5'- TTGCTCTACCGGGGACTG -3'

Posted On

2014-05-23