Mutagenetix > Incidental Mutation

Incidental Mutation 'R1734:Vmn1r20'

199560

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r20

Ensembl Gene

ENSMUSG00000115253

Gene Name

vomeronasal 1 receptor 20

Synonyms

Gm5569

MMRRC Submission

039766-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.459) question?

Stock #

R1734 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

57428930-57435261 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 57432300 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 204 (R204G)

Ref Sequence

ENSEMBL: ENSMUSP00000153815 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176085] [ENSMUST00000226808] [ENSMUST00000227909]

AlphaFold

K7N778

Predicted Effect

probably damaging
Transcript: ENSMUST00000176085
AA Change: R204G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134973
Gene: ENSMUSG00000093679
AA Change: R204G

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	2.3e-51	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000226808
AA Change: R204G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000227909
AA Change: R204G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.5%
20x: 92.9%

Validation Efficiency

98% (59/60)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,585,460 (GRCm38)	C4695R	probably benign	Het
Actr10	T	A	12: 70,961,996 (GRCm38)	V401E	probably benign	Het
Adamts16	G	A	13: 70,779,518 (GRCm38)		probably benign	Het
Aimp1	A	T	3: 132,674,796 (GRCm38)	I59K	probably damaging	Het
Alms1	T	A	6: 85,641,550 (GRCm38)		probably null	Het
Anln	A	T	9: 22,350,955 (GRCm38)	S947T	possibly damaging	Het
Atp2c1	T	C	9: 105,414,655 (GRCm38)	T733A	probably damaging	Het
BC049715	C	T	6: 136,840,308 (GRCm38)	P182L	probably damaging	Het
Cblb	T	C	16: 52,186,240 (GRCm38)		probably benign	Het
Cep295	C	T	9: 15,340,883 (GRCm38)	E397K	probably damaging	Het
Ces4a	G	A	8: 105,138,097 (GRCm38)	G69S	probably damaging	Het
Chac1	A	T	2: 119,353,458 (GRCm38)	L180F	probably damaging	Het
Cherp	A	T	8: 72,470,088 (GRCm38)		probably null	Het
Ckap4	A	G	10: 84,527,874 (GRCm38)	S442P	probably benign	Het
Clstn3	G	A	6: 124,436,814 (GRCm38)		probably benign	Het
Crb2	T	A	2: 37,793,656 (GRCm38)	C1057S	probably damaging	Het
Dact2	T	C	17: 14,196,639 (GRCm38)	D433G	probably benign	Het
Dnah6	G	A	6: 73,044,761 (GRCm38)	T3526M	probably damaging	Het
Ethe1	A	G	7: 24,608,384 (GRCm38)	T210A	probably benign	Het
Fat2	A	G	11: 55,281,371 (GRCm38)	S2839P	probably benign	Het
Fbxl7	T	C	15: 26,543,649 (GRCm38)	Y304C	probably damaging	Het
Gad1-ps	A	T	10: 99,445,775 (GRCm38)		noncoding transcript	Het
Gm436	A	T	4: 144,670,026 (GRCm38)	C379S	probably benign	Het
Grm3	C	T	5: 9,589,742 (GRCm38)	R101K	probably benign	Het
Hspa12b	A	G	2: 131,138,536 (GRCm38)	Y125C	possibly damaging	Het
Il10ra	T	C	9: 45,255,943 (GRCm38)	T437A	probably benign	Het
Jcad	T	C	18: 4,674,526 (GRCm38)	F763L	probably damaging	Het
Map3k10	T	C	7: 27,658,115 (GRCm38)	D746G	probably damaging	Het
Mettl9	T	A	7: 121,047,841 (GRCm38)	Y57N	probably damaging	Het
Nav2	G	A	7: 49,575,720 (GRCm38)	E1803K	probably damaging	Het
Nol11	G	A	11: 107,175,623 (GRCm38)	S447L	possibly damaging	Het
Olfr294	C	T	7: 86,616,217 (GRCm38)	V143M	probably benign	Het
Osbpl1a	T	C	18: 12,788,316 (GRCm38)		probably null	Het
Pde6a	A	G	18: 61,285,965 (GRCm38)	N804S	probably damaging	Het
Pepd	A	T	7: 35,031,426 (GRCm38)	D301V	probably benign	Het
Piwil2	A	T	14: 70,426,505 (GRCm38)		probably null	Het
Plec	C	T	15: 76,186,218 (GRCm38)	V931M	probably damaging	Het
Prrc2a	A	G	17: 35,150,707 (GRCm38)	S1877P	possibly damaging	Het
Retreg2	G	T	1: 75,142,986 (GRCm38)		probably null	Het
Slc7a11	G	A	3: 50,372,346 (GRCm38)	Q489*	probably null	Het
Sned1	G	A	1: 93,259,768 (GRCm38)	D256N	probably damaging	Het
Sphkap	G	A	1: 83,277,515 (GRCm38)	R838*	probably null	Het
Ssfa2	G	A	2: 79,657,822 (GRCm38)	V750M	probably damaging	Het
Syce2	G	A	8: 84,887,147 (GRCm38)	E168K	probably benign	Het
Tex37	T	A	6: 70,913,661 (GRCm38)	Q49L	probably benign	Het
Tmem260	G	T	14: 48,509,093 (GRCm38)	V609L	probably benign	Het
Trim35	A	G	14: 66,309,329 (GRCm38)	D515G	probably damaging	Het
Tspan5	T	C	3: 138,898,140 (GRCm38)	Y131H	probably damaging	Het
Ttbk2	T	C	2: 120,755,838 (GRCm38)	I466V	probably benign	Het
Ttn	T	C	2: 76,745,813 (GRCm38)	D24912G	probably damaging	Het
Utp20	A	T	10: 88,767,461 (GRCm38)	N1843K	probably damaging	Het
Vps18	A	T	2: 119,293,942 (GRCm38)	Q450L	probably benign	Het
Zbtb4	A	G	11: 69,776,463 (GRCm38)	E198G	probably benign	Het

Other mutations in Vmn1r20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01761:Vmn1r20	APN	6	57,431,740 (GRCm38)	missense	probably damaging	1.00
IGL02622:Vmn1r20	APN	6	57,432,598 (GRCm38)	missense	probably damaging	0.99
IGL02636:Vmn1r20	APN	6	57,431,761 (GRCm38)	missense	probably benign
R0006:Vmn1r20	UTSW	6	57,432,305 (GRCm38)	missense	probably damaging	0.99
R0079:Vmn1r20	UTSW	6	57,431,792 (GRCm38)	missense	possibly damaging	0.70
R0200:Vmn1r20	UTSW	6	57,432,099 (GRCm38)	missense	probably damaging	0.96
R1675:Vmn1r20	UTSW	6	57,431,952 (GRCm38)	missense	probably benign	0.00
R1883:Vmn1r20	UTSW	6	57,432,321 (GRCm38)	missense	probably benign	0.14
R2049:Vmn1r20	UTSW	6	57,431,958 (GRCm38)	missense	probably damaging	1.00
R2307:Vmn1r20	UTSW	6	57,432,136 (GRCm38)	missense	probably benign	0.03
R2346:Vmn1r20	UTSW	6	57,431,946 (GRCm38)	missense	possibly damaging	0.83
R4405:Vmn1r20	UTSW	6	57,432,057 (GRCm38)	missense	probably benign	0.02
R4946:Vmn1r20	UTSW	6	57,432,174 (GRCm38)	missense	probably damaging	0.98
R5259:Vmn1r20	UTSW	6	57,432,065 (GRCm38)	nonsense	probably null
R5321:Vmn1r20	UTSW	6	57,432,442 (GRCm38)	missense	probably benign	0.21
R5911:Vmn1r20	UTSW	6	57,431,789 (GRCm38)	missense	probably benign	0.16
R6042:Vmn1r20	UTSW	6	57,432,406 (GRCm38)	missense	possibly damaging	0.90
R6298:Vmn1r20	UTSW	6	57,432,127 (GRCm38)	missense	probably benign
R6314:Vmn1r20	UTSW	6	57,432,057 (GRCm38)	missense	probably benign	0.00
R6331:Vmn1r20	UTSW	6	57,431,670 (GRCm38)	splice site	probably null
R6482:Vmn1r20	UTSW	6	57,432,108 (GRCm38)	missense	probably benign	0.01
R9161:Vmn1r20	UTSW	6	57,431,774 (GRCm38)	missense	probably benign	0.00
R9165:Vmn1r20	UTSW	6	57,432,261 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TTCCAGGCTGTCACAATCAGTCCC -3'
(R):5'- ACCCTCAGGATGACTGTGTCATACC -3'

Sequencing Primer
(F):5'- CTACAGTAGTAGCCGGATCTTCTATG -3'
(R):5'- GGATGACTGTGTCATACCTCCAC -3'

Posted On

2014-05-23