Mutagenetix > Incidental Mutation

Incidental Mutation 'R1737:Or10k2'

199787

Institutional Source

Beutler Lab

Gene Symbol

Or10k2

Ensembl Gene

ENSMUSG00000047286

Gene Name

olfactory receptor family 10 subfamily K member 2

Synonyms

Olfr370, GA_x6K02T2NUPS-11609807-11610751, MOR267-16

MMRRC Submission

039769-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.136) question?

Stock #

R1737 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84267722-84268817 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 84268045 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 91 (S91G)

Ref Sequence

ENSEMBL: ENSMUSP00000149251 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058609] [ENSMUST00000209408] [ENSMUST00000214156]

AlphaFold

E9Q848

Predicted Effect

probably benign
Transcript: ENSMUST00000058609
AA Change: S91G

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000055435
Gene: ENSMUSG00000047286
AA Change: S91G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.6e-44	PFAM
Pfam:7tm_1	41	290	6.2e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000209408
AA Change: S91G

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

Predicted Effect

probably benign
Transcript: ENSMUST00000214156
AA Change: S91G

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.6%
3x: 96.9%
10x: 95.1%
20x: 91.4%

Validation Efficiency

100% (75/75)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Ak7	G	A	12: 105,708,591 (GRCm39)	V349M	probably damaging	Het
Aldh1a2	A	T	9: 71,192,453 (GRCm39)	E436V	possibly damaging	Het
Apc	C	A	18: 34,450,075 (GRCm39)	P2290T	probably damaging	Het
Atp10a	T	C	7: 58,476,986 (GRCm39)		probably benign	Het
Bahd1	T	C	2: 118,746,404 (GRCm39)	S8P	probably damaging	Het
BC049715	C	T	6: 136,817,306 (GRCm39)	P182L	probably damaging	Het
Capn10	T	C	1: 92,862,677 (GRCm39)	Y28H	probably benign	Het
Cdh10	A	T	15: 18,964,149 (GRCm39)	I104F	probably damaging	Het
Cep170b	A	G	12: 112,703,061 (GRCm39)	D508G	possibly damaging	Het
Ces2a	G	T	8: 105,467,456 (GRCm39)	A494S	probably damaging	Het
Ces4a	G	A	8: 105,864,729 (GRCm39)	G69S	probably damaging	Het
Cltc	G	A	11: 86,624,553 (GRCm39)	T123M	probably damaging	Het
Cnot1	A	G	8: 96,474,904 (GRCm39)	S1102P	probably damaging	Het
Col12a1	C	A	9: 79,610,733 (GRCm39)	D224Y	probably damaging	Het
Col14a1	T	A	15: 55,208,357 (GRCm39)		probably benign	Het
Cyp7a1	T	A	4: 6,272,848 (GRCm39)	T122S	probably benign	Het
Dock4	A	G	12: 40,857,000 (GRCm39)		probably null	Het
Eid2	T	C	7: 27,967,924 (GRCm39)	V182A	possibly damaging	Het
Eloa	A	T	4: 135,738,081 (GRCm39)	V293D	probably benign	Het
Enkur	G	A	2: 21,199,106 (GRCm39)	P143S	probably damaging	Het
Ep300	T	C	15: 81,510,548 (GRCm39)	Y99H	probably damaging	Het
Fbxw5	C	A	2: 25,393,596 (GRCm39)	L66I	probably benign	Het
Flg2	A	T	3: 93,110,928 (GRCm39)	R985S	unknown	Het
Fscb	A	T	12: 64,521,355 (GRCm39)	I37K	possibly damaging	Het
H2-M10.3	T	C	17: 36,679,296 (GRCm39)	N3S	probably benign	Het
H2-Q7	A	C	17: 35,658,602 (GRCm39)	Y80S	probably damaging	Het
H60c	A	T	10: 3,209,914 (GRCm39)	D124E	possibly damaging	Het
Hsd3b2	A	T	3: 98,618,862 (GRCm39)	V361E	probably damaging	Het
Ice1	A	C	13: 70,754,444 (GRCm39)	C547W	probably damaging	Het
Itprid1	A	T	6: 55,945,289 (GRCm39)	D670V	probably damaging	Het
Jmy	A	G	13: 93,635,303 (GRCm39)	V171A	probably damaging	Het
Klf7	C	A	1: 64,118,372 (GRCm39)	R75L	possibly damaging	Het
Klk1b1	A	T	7: 43,619,783 (GRCm39)	Q114L	probably benign	Het
Krt10	A	G	11: 99,278,213 (GRCm39)	M280T	possibly damaging	Het
Lama1	G	A	17: 68,109,916 (GRCm39)	R2179Q	probably benign	Het
Mbd3l1	C	T	9: 18,396,225 (GRCm39)	P117S	possibly damaging	Het
Med18	A	T	4: 132,187,420 (GRCm39)	S71R	probably damaging	Het
Mov10l1	T	A	15: 88,895,607 (GRCm39)	I643N	possibly damaging	Het
Nfib	T	C	4: 82,416,826 (GRCm39)	K70E	probably damaging	Het
Nr3c2	A	C	8: 77,634,958 (GRCm39)	S20R	probably benign	Het
Nr6a1	A	T	2: 38,628,955 (GRCm39)	F356L	probably benign	Het
Oca2	G	A	7: 55,978,533 (GRCm39)	R567H	probably damaging	Het
Or1e35	T	A	11: 73,797,911 (GRCm39)	M136L	possibly damaging	Het
Or1p1	T	C	11: 74,179,637 (GRCm39)	L55P	probably damaging	Het
Or6c88	T	A	10: 129,406,697 (GRCm39)	Y58N	probably damaging	Het
Or8g33	T	C	9: 39,338,254 (GRCm39)	T38A	probably damaging	Het
Pcdhb10	A	G	18: 37,546,009 (GRCm39)	T362A	probably benign	Het
Pip5k1b	A	T	19: 24,374,404 (GRCm39)	V59E	probably damaging	Het
Pkhd1l1	G	A	15: 44,410,905 (GRCm39)		probably null	Het
Prr36	T	C	8: 4,264,370 (GRCm39)		probably benign	Het
Ptger2	A	G	14: 45,239,228 (GRCm39)	T289A	probably benign	Het
Rad18	A	G	6: 112,658,498 (GRCm39)	F136L	probably damaging	Het
Rhbg	T	A	3: 88,153,181 (GRCm39)	S258C	probably damaging	Het
Scgb2b27	T	C	7: 33,712,739 (GRCm39)	I34M	possibly damaging	Het
Senp7	C	A	16: 55,944,162 (GRCm39)	T135K	probably damaging	Het
Slc20a2	A	G	8: 23,035,582 (GRCm39)	I193M	probably damaging	Het
Slc27a1	G	A	8: 72,023,504 (GRCm39)	V47I	probably benign	Het
Slc9a5	G	A	8: 106,094,766 (GRCm39)	V786M	probably damaging	Het
Smyd1	A	G	6: 71,193,875 (GRCm39)	L350P	probably damaging	Het
Ston2	A	T	12: 91,614,681 (GRCm39)	F576I	probably damaging	Het
Sulf2	A	T	2: 165,924,598 (GRCm39)	V496E	probably benign	Het
Tcl1b3	C	A	12: 105,157,481 (GRCm39)	S41Y	probably damaging	Het
Tulp2	A	T	7: 45,169,201 (GRCm39)	N256I	probably damaging	Het
Uspl1	T	A	5: 149,138,668 (GRCm39)	C349S	probably damaging	Het
Vcl	T	C	14: 21,070,604 (GRCm39)	I730T	probably damaging	Het
Vmn1r61	A	T	7: 5,614,060 (GRCm39)	F85I	probably benign	Het
Zfp318	A	G	17: 46,710,403 (GRCm39)	T709A	probably benign	Het
Zfr2	T	C	10: 81,077,919 (GRCm39)	S249P	probably damaging	Het

Other mutations in Or10k2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Or10k2	APN	8	84,268,247 (GRCm39)	missense	probably benign	0.00
IGL02481:Or10k2	APN	8	84,268,015 (GRCm39)	missense	possibly damaging	0.70
IGL02499:Or10k2	APN	8	84,267,812 (GRCm39)	missense	possibly damaging	0.83
IGL02832:Or10k2	APN	8	84,268,100 (GRCm39)	missense	probably damaging	1.00
R0315:Or10k2	UTSW	8	84,268,001 (GRCm39)	missense	possibly damaging	0.90
R0330:Or10k2	UTSW	8	84,268,142 (GRCm39)	missense	probably damaging	1.00
R1973:Or10k2	UTSW	8	84,268,421 (GRCm39)	missense	probably benign	0.00
R2157:Or10k2	UTSW	8	84,268,250 (GRCm39)	missense	probably benign	0.00
R4687:Or10k2	UTSW	8	84,268,489 (GRCm39)	missense	probably damaging	1.00
R5639:Or10k2	UTSW	8	84,267,973 (GRCm39)	missense	probably damaging	1.00
R6150:Or10k2	UTSW	8	84,267,782 (GRCm39)	missense	probably benign	0.02
R6590:Or10k2	UTSW	8	84,267,904 (GRCm39)	missense	probably benign	0.08
R6682:Or10k2	UTSW	8	84,268,187 (GRCm39)	missense	probably benign	0.00
R6690:Or10k2	UTSW	8	84,267,904 (GRCm39)	missense	probably benign	0.08
R7846:Or10k2	UTSW	8	84,268,526 (GRCm39)	missense	probably damaging	1.00
R7886:Or10k2	UTSW	8	84,268,576 (GRCm39)	missense	possibly damaging	0.95
R8349:Or10k2	UTSW	8	84,268,301 (GRCm39)	missense	probably damaging	1.00
R8361:Or10k2	UTSW	8	84,268,715 (GRCm39)	missense	possibly damaging	0.86
R8449:Or10k2	UTSW	8	84,268,301 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGTAAGGATTTCCTCAGTGCCCTC -3'
(R):5'- TTACCTGTGCCACAGTGAAGCC -3'

Sequencing Primer
(F):5'- CTGATCTCTGATGACAGAGCC -3'
(R):5'- GTGAAGCCACAGACACACG -3'

Posted On

2014-05-23