Mutagenetix > Incidental Mutation

Incidental Mutation 'R1737:Apc'

199824

Institutional Source

Beutler Lab

Gene Symbol

Apc

Ensembl Gene

ENSMUSG00000005871

Gene Name

APC, WNT signaling pathway regulator

Synonyms

Min, adenomatosis polyposis coli, CC1

MMRRC Submission

039769-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.970) question?

Stock #

R1737 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

34353977-34455605 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 34450075 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Threonine at position 2290 (P2290T)

Ref Sequence

ENSEMBL: ENSMUSP00000111447 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079362] [ENSMUST00000115781] [ENSMUST00000171187]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000079362
AA Change: P2324T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078337
Gene: ENSMUSG00000005871
AA Change: P2324T

Domain	Start	End	E-Value	Type
Pfam:APC_N_CC	4	55	6e-32	PFAM
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	125	205	2e-24	PFAM
low complexity region	211	222	N/A	INTRINSIC
low complexity region	234	247	N/A	INTRINSIC
ARM	338	390	6.14e-5	SMART
ARM	457	508	1.62e-4	SMART
ARM	510	551	8.56e-4	SMART
ARM	554	595	4.45e-2	SMART
ARM	597	642	5.76e1	SMART
ARM	647	687	1.29e-7	SMART
Pfam:Arm_APC_u3	730	1017	5e-170	PFAM
Pfam:APC_15aa	1018	1032	1.1e-8	PFAM
Pfam:APC_u5	1034	1133	7.6e-55	PFAM
Pfam:APC_15aa	1154	1168	1.6e-8	PFAM
Pfam:APC_15aa	1171	1185	1.9e-9	PFAM
low complexity region	1187	1204	N/A	INTRINSIC
Pfam:APC_crr	1255	1279	1.5e-15	PFAM
Pfam:APC_u9	1280	1367	1.9e-34	PFAM
Pfam:APC_crr	1370	1393	2.2e-10	PFAM
low complexity region	1431	1449	N/A	INTRINSIC
Pfam:APC_crr	1485	1509	2.1e-9	PFAM
low complexity region	1532	1548	N/A	INTRINSIC
Pfam:SAMP	1568	1587	2.7e-11	PFAM
Pfam:APC_crr	1635	1659	1.9e-15	PFAM
Pfam:APC_u13	1660	1716	1.3e-31	PFAM
Pfam:SAMP	1717	1736	3.2e-12	PFAM
Pfam:APC_u14	1737	1837	1e-46	PFAM
Pfam:APC_crr	1839	1864	6.8e-15	PFAM
Pfam:APC_u15	1865	1945	1.8e-40	PFAM
Pfam:APC_crr	1947	1971	1.6e-14	PFAM
Pfam:APC_crr	2007	2030	1.8e-14	PFAM
Pfam:SAMP	2033	2052	1.6e-13	PFAM
low complexity region	2112	2146	N/A	INTRINSIC
Pfam:APC_basic	2223	2579	1.5e-110	PFAM
low complexity region	2626	2638	N/A	INTRINSIC
Pfam:EB1_binding	2670	2842	9.3e-89	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000115781
AA Change: P2290T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111447
Gene: ENSMUSG00000005871
AA Change: P2290T

Domain	Start	End	E-Value	Type
PDB:1DEB\|B	2	55	1e-27	PDB
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	124	206	1.5e-31	PFAM
low complexity region	211	222	N/A	INTRINSIC
ARM	304	356	6.14e-5	SMART
ARM	423	474	1.62e-4	SMART
ARM	476	517	8.56e-4	SMART
ARM	520	561	4.45e-2	SMART
ARM	563	608	5.76e1	SMART
ARM	613	653	1.29e-7	SMART
Pfam:Arm	655	695	1.7e-6	PFAM
low complexity region	797	810	N/A	INTRINSIC
low complexity region	880	892	N/A	INTRINSIC
low complexity region	923	935	N/A	INTRINSIC
Pfam:APC_15aa	984	999	3.7e-9	PFAM
Pfam:APC_15aa	1100	1115	8.4e-8	PFAM
Pfam:APC_15aa	1120	1135	9.9e-9	PFAM
Pfam:APC_15aa	1137	1152	1.2e-9	PFAM
low complexity region	1153	1170	N/A	INTRINSIC
Pfam:APC_crr	1220	1245	7.5e-15	PFAM
low complexity region	1320	1331	N/A	INTRINSIC
Pfam:APC_crr	1334	1359	2.8e-11	PFAM
low complexity region	1397	1415	N/A	INTRINSIC
Pfam:APC_crr	1450	1475	2.2e-8	PFAM
low complexity region	1498	1514	N/A	INTRINSIC
Pfam:SAMP	1533	1553	8.4e-12	PFAM
Pfam:APC_crr	1600	1625	3.5e-13	PFAM
Pfam:SAMP	1682	1702	5e-12	PFAM
low complexity region	1732	1744	N/A	INTRINSIC
Pfam:APC_crr	1805	1830	3.1e-12	PFAM
low complexity region	1866	1877	N/A	INTRINSIC
Pfam:APC_crr	1912	1937	3.5e-13	PFAM
Pfam:APC_crr	1971	1996	7.1e-14	PFAM
Pfam:SAMP	1999	2018	4.6e-13	PFAM
low complexity region	2078	2112	N/A	INTRINSIC
Pfam:APC_basic	2189	2545	1.1e-131	PFAM
low complexity region	2592	2604	N/A	INTRINSIC
Pfam:EB1_binding	2636	2808	2.9e-90	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000165590

SMART Domains

Protein: ENSMUSP00000128327
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
PDB:3AU3\|A	2	33	2e-17	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000171187

SMART Domains

Protein: ENSMUSP00000127131
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
low complexity region	9	20	N/A	INTRINSIC
low complexity region	23	52	N/A	INTRINSIC
low complexity region	102	119	N/A	INTRINSIC
Pfam:Suppressor_APC	134	216	5.2e-32	PFAM
ARM	320	372	6.14e-5	SMART
ARM	439	490	1.62e-4	SMART
ARM	492	533	8.56e-4	SMART
ARM	536	577	4.45e-2	SMART
ARM	579	624	5.76e1	SMART
ARM	629	669	1.29e-7	SMART
Pfam:Arm	671	711	6.3e-7	PFAM
low complexity region	813	826	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	939	951	N/A	INTRINSIC
Pfam:APC_15aa	1000	1015	1.4e-9	PFAM
Pfam:APC_15aa	1116	1131	3.1e-8	PFAM

Meta Mutation Damage Score

0.2386

Coding Region Coverage

1x: 97.6%
3x: 96.9%
10x: 95.1%
20x: 91.4%

Validation Efficiency

100% (75/75)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
PHENOTYPE: Most targeted and hypomorphic heterozygous mutants develop intestinal polyps and colorectal cancer, associated with anemia from intestinal bleeding. Homozygotes are embryonic lethal. Homozygotes for a mild alleles survive and have less extreme tumor incidence. [provided by MGI curators]

Allele List at MGI

All alleles(88) : Targeted(25) Gene trapped(62) Chemically induced(1)

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Ak7	G	A	12: 105,708,591 (GRCm39)	V349M	probably damaging	Het
Aldh1a2	A	T	9: 71,192,453 (GRCm39)	E436V	possibly damaging	Het
Atp10a	T	C	7: 58,476,986 (GRCm39)		probably benign	Het
Bahd1	T	C	2: 118,746,404 (GRCm39)	S8P	probably damaging	Het
BC049715	C	T	6: 136,817,306 (GRCm39)	P182L	probably damaging	Het
Capn10	T	C	1: 92,862,677 (GRCm39)	Y28H	probably benign	Het
Cdh10	A	T	15: 18,964,149 (GRCm39)	I104F	probably damaging	Het
Cep170b	A	G	12: 112,703,061 (GRCm39)	D508G	possibly damaging	Het
Ces2a	G	T	8: 105,467,456 (GRCm39)	A494S	probably damaging	Het
Ces4a	G	A	8: 105,864,729 (GRCm39)	G69S	probably damaging	Het
Cltc	G	A	11: 86,624,553 (GRCm39)	T123M	probably damaging	Het
Cnot1	A	G	8: 96,474,904 (GRCm39)	S1102P	probably damaging	Het
Col12a1	C	A	9: 79,610,733 (GRCm39)	D224Y	probably damaging	Het
Col14a1	T	A	15: 55,208,357 (GRCm39)		probably benign	Het
Cyp7a1	T	A	4: 6,272,848 (GRCm39)	T122S	probably benign	Het
Dock4	A	G	12: 40,857,000 (GRCm39)		probably null	Het
Eid2	T	C	7: 27,967,924 (GRCm39)	V182A	possibly damaging	Het
Eloa	A	T	4: 135,738,081 (GRCm39)	V293D	probably benign	Het
Enkur	G	A	2: 21,199,106 (GRCm39)	P143S	probably damaging	Het
Ep300	T	C	15: 81,510,548 (GRCm39)	Y99H	probably damaging	Het
Fbxw5	C	A	2: 25,393,596 (GRCm39)	L66I	probably benign	Het
Flg2	A	T	3: 93,110,928 (GRCm39)	R985S	unknown	Het
Fscb	A	T	12: 64,521,355 (GRCm39)	I37K	possibly damaging	Het
H2-M10.3	T	C	17: 36,679,296 (GRCm39)	N3S	probably benign	Het
H2-Q7	A	C	17: 35,658,602 (GRCm39)	Y80S	probably damaging	Het
H60c	A	T	10: 3,209,914 (GRCm39)	D124E	possibly damaging	Het
Hsd3b2	A	T	3: 98,618,862 (GRCm39)	V361E	probably damaging	Het
Ice1	A	C	13: 70,754,444 (GRCm39)	C547W	probably damaging	Het
Itprid1	A	T	6: 55,945,289 (GRCm39)	D670V	probably damaging	Het
Jmy	A	G	13: 93,635,303 (GRCm39)	V171A	probably damaging	Het
Klf7	C	A	1: 64,118,372 (GRCm39)	R75L	possibly damaging	Het
Klk1b1	A	T	7: 43,619,783 (GRCm39)	Q114L	probably benign	Het
Krt10	A	G	11: 99,278,213 (GRCm39)	M280T	possibly damaging	Het
Lama1	G	A	17: 68,109,916 (GRCm39)	R2179Q	probably benign	Het
Mbd3l1	C	T	9: 18,396,225 (GRCm39)	P117S	possibly damaging	Het
Med18	A	T	4: 132,187,420 (GRCm39)	S71R	probably damaging	Het
Mov10l1	T	A	15: 88,895,607 (GRCm39)	I643N	possibly damaging	Het
Nfib	T	C	4: 82,416,826 (GRCm39)	K70E	probably damaging	Het
Nr3c2	A	C	8: 77,634,958 (GRCm39)	S20R	probably benign	Het
Nr6a1	A	T	2: 38,628,955 (GRCm39)	F356L	probably benign	Het
Oca2	G	A	7: 55,978,533 (GRCm39)	R567H	probably damaging	Het
Or10k2	A	G	8: 84,268,045 (GRCm39)	S91G	probably benign	Het
Or1e35	T	A	11: 73,797,911 (GRCm39)	M136L	possibly damaging	Het
Or1p1	T	C	11: 74,179,637 (GRCm39)	L55P	probably damaging	Het
Or6c88	T	A	10: 129,406,697 (GRCm39)	Y58N	probably damaging	Het
Or8g33	T	C	9: 39,338,254 (GRCm39)	T38A	probably damaging	Het
Pcdhb10	A	G	18: 37,546,009 (GRCm39)	T362A	probably benign	Het
Pip5k1b	A	T	19: 24,374,404 (GRCm39)	V59E	probably damaging	Het
Pkhd1l1	G	A	15: 44,410,905 (GRCm39)		probably null	Het
Prr36	T	C	8: 4,264,370 (GRCm39)		probably benign	Het
Ptger2	A	G	14: 45,239,228 (GRCm39)	T289A	probably benign	Het
Rad18	A	G	6: 112,658,498 (GRCm39)	F136L	probably damaging	Het
Rhbg	T	A	3: 88,153,181 (GRCm39)	S258C	probably damaging	Het
Scgb2b27	T	C	7: 33,712,739 (GRCm39)	I34M	possibly damaging	Het
Senp7	C	A	16: 55,944,162 (GRCm39)	T135K	probably damaging	Het
Slc20a2	A	G	8: 23,035,582 (GRCm39)	I193M	probably damaging	Het
Slc27a1	G	A	8: 72,023,504 (GRCm39)	V47I	probably benign	Het
Slc9a5	G	A	8: 106,094,766 (GRCm39)	V786M	probably damaging	Het
Smyd1	A	G	6: 71,193,875 (GRCm39)	L350P	probably damaging	Het
Ston2	A	T	12: 91,614,681 (GRCm39)	F576I	probably damaging	Het
Sulf2	A	T	2: 165,924,598 (GRCm39)	V496E	probably benign	Het
Tcl1b3	C	A	12: 105,157,481 (GRCm39)	S41Y	probably damaging	Het
Tulp2	A	T	7: 45,169,201 (GRCm39)	N256I	probably damaging	Het
Uspl1	T	A	5: 149,138,668 (GRCm39)	C349S	probably damaging	Het
Vcl	T	C	14: 21,070,604 (GRCm39)	I730T	probably damaging	Het
Vmn1r61	A	T	7: 5,614,060 (GRCm39)	F85I	probably benign	Het
Zfp318	A	G	17: 46,710,403 (GRCm39)	T709A	probably benign	Het
Zfr2	T	C	10: 81,077,919 (GRCm39)	S249P	probably damaging	Het

Other mutations in Apc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00507:Apc	APN	18	34,449,979 (GRCm39)	missense	probably benign	0.01
IGL00898:Apc	APN	18	34,450,147 (GRCm39)	missense	probably damaging	1.00
IGL01111:Apc	APN	18	34,448,189 (GRCm39)	missense	possibly damaging	0.95
IGL01347:Apc	APN	18	34,450,723 (GRCm39)	missense	probably damaging	1.00
IGL01375:Apc	APN	18	34,446,707 (GRCm39)	missense	probably damaging	1.00
IGL01805:Apc	APN	18	34,451,271 (GRCm39)	missense	probably benign	0.02
IGL01997:Apc	APN	18	34,448,476 (GRCm39)	missense	probably benign	0.00
IGL02033:Apc	APN	18	34,443,772 (GRCm39)	missense	probably damaging	1.00
IGL02323:Apc	APN	18	34,448,863 (GRCm39)	nonsense	probably null
IGL02373:Apc	APN	18	34,449,212 (GRCm39)	missense	probably damaging	1.00
IGL02379:Apc	APN	18	34,431,798 (GRCm39)	missense	probably benign	0.45
IGL02456:Apc	APN	18	34,446,935 (GRCm39)	nonsense	probably null
IGL02552:Apc	APN	18	34,446,035 (GRCm39)	missense	possibly damaging	0.90
IGL02676:Apc	APN	18	34,448,687 (GRCm39)	missense	probably damaging	1.00
IGL02756:Apc	APN	18	34,447,588 (GRCm39)	missense	probably damaging	1.00
IGL02938:Apc	APN	18	34,448,281 (GRCm39)	missense	probably damaging	0.98
IGL02974:Apc	APN	18	34,401,436 (GRCm39)	splice site	probably benign
IGL03124:Apc	APN	18	34,433,038 (GRCm39)	missense	probably damaging	0.98
IGL03201:Apc	APN	18	34,445,429 (GRCm39)	missense	probably damaging	1.00
IGL03339:Apc	APN	18	34,431,527 (GRCm39)	missense	probably damaging	1.00
FR4304:Apc	UTSW	18	34,415,050 (GRCm39)	intron	probably benign
FR4342:Apc	UTSW	18	34,415,052 (GRCm39)	intron	probably benign
FR4449:Apc	UTSW	18	34,415,058 (GRCm39)	intron	probably benign
FR4449:Apc	UTSW	18	34,415,053 (GRCm39)	intron	probably benign
FR4548:Apc	UTSW	18	34,415,051 (GRCm39)	intron	probably benign
FR4737:Apc	UTSW	18	34,415,052 (GRCm39)	intron	probably benign
FR4976:Apc	UTSW	18	34,415,057 (GRCm39)	nonsense	probably null
FR4976:Apc	UTSW	18	34,415,053 (GRCm39)	intron	probably benign
FR4976:Apc	UTSW	18	34,415,051 (GRCm39)	intron	probably benign
R0385:Apc	UTSW	18	34,448,997 (GRCm39)	missense	probably damaging	1.00
R0535:Apc	UTSW	18	34,394,125 (GRCm39)	missense	probably damaging	1.00
R0561:Apc	UTSW	18	34,446,356 (GRCm39)	missense	possibly damaging	0.94
R0590:Apc	UTSW	18	34,449,283 (GRCm39)	nonsense	probably null
R0626:Apc	UTSW	18	34,451,507 (GRCm39)	missense	probably damaging	1.00
R0991:Apc	UTSW	18	34,449,160 (GRCm39)	missense	probably damaging	1.00
R1564:Apc	UTSW	18	34,448,202 (GRCm39)	missense	probably benign	0.00
R1663:Apc	UTSW	18	34,401,378 (GRCm39)	missense	probably damaging	0.98
R1739:Apc	UTSW	18	34,445,371 (GRCm39)	missense	probably damaging	1.00
R1835:Apc	UTSW	18	34,450,130 (GRCm39)	missense	probably damaging	1.00
R1887:Apc	UTSW	18	34,405,521 (GRCm39)	missense	probably damaging	1.00
R1957:Apc	UTSW	18	34,450,388 (GRCm39)	missense	probably damaging	1.00
R1974:Apc	UTSW	18	34,433,057 (GRCm39)	missense	possibly damaging	0.62
R2005:Apc	UTSW	18	34,443,962 (GRCm39)	critical splice donor site	probably null
R2013:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2014:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2015:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2017:Apc	UTSW	18	34,446,655 (GRCm39)	missense	probably benign	0.00
R2056:Apc	UTSW	18	34,449,481 (GRCm39)	missense	probably damaging	1.00
R2108:Apc	UTSW	18	34,402,282 (GRCm39)	missense	probably damaging	1.00
R2120:Apc	UTSW	18	34,409,654 (GRCm39)	missense	probably damaging	1.00
R2131:Apc	UTSW	18	34,445,098 (GRCm39)	missense	possibly damaging	0.51
R2133:Apc	UTSW	18	34,445,098 (GRCm39)	missense	possibly damaging	0.51
R2291:Apc	UTSW	18	34,445,544 (GRCm39)	missense	probably benign	0.45
R2332:Apc	UTSW	18	34,450,112 (GRCm39)	missense	possibly damaging	0.50
R2360:Apc	UTSW	18	34,394,179 (GRCm39)	missense	probably damaging	1.00
R2407:Apc	UTSW	18	34,447,315 (GRCm39)	missense	possibly damaging	0.77
R2507:Apc	UTSW	18	34,449,590 (GRCm39)	missense	possibly damaging	0.77
R2940:Apc	UTSW	18	34,409,723 (GRCm39)	missense	probably damaging	1.00
R3404:Apc	UTSW	18	34,446,655 (GRCm39)	missense	probably benign	0.00
R3411:Apc	UTSW	18	34,402,312 (GRCm39)	splice site	probably benign
R3778:Apc	UTSW	18	34,446,134 (GRCm39)	missense	probably damaging	1.00
R3826:Apc	UTSW	18	34,412,388 (GRCm39)	missense	possibly damaging	0.93
R4599:Apc	UTSW	18	34,451,040 (GRCm39)	nonsense	probably null
R4611:Apc	UTSW	18	34,451,618 (GRCm39)	missense	probably damaging	1.00
R4664:Apc	UTSW	18	34,431,647 (GRCm39)	missense	probably damaging	0.98
R4969:Apc	UTSW	18	34,445,971 (GRCm39)	nonsense	probably null
R5007:Apc	UTSW	18	34,446,016 (GRCm39)	missense	probably damaging	1.00
R5066:Apc	UTSW	18	34,449,158 (GRCm39)	missense	probably damaging	1.00
R5112:Apc	UTSW	18	34,449,162 (GRCm39)	nonsense	probably null
R5259:Apc	UTSW	18	34,447,343 (GRCm39)	missense	probably benign	0.29
R5440:Apc	UTSW	18	34,354,213 (GRCm39)	unclassified	probably benign
R5508:Apc	UTSW	18	34,431,633 (GRCm39)	missense	probably damaging	0.97
R5512:Apc	UTSW	18	34,443,962 (GRCm39)	critical splice donor site	probably benign
R5850:Apc	UTSW	18	34,451,116 (GRCm39)	missense	possibly damaging	0.94
R5951:Apc	UTSW	18	34,450,199 (GRCm39)	missense	possibly damaging	0.89
R5966:Apc	UTSW	18	34,354,140 (GRCm39)	utr 5 prime	probably benign
R6081:Apc	UTSW	18	34,423,164 (GRCm39)	missense	possibly damaging	0.93
R6116:Apc	UTSW	18	34,449,508 (GRCm39)	missense	probably damaging	1.00
R6351:Apc	UTSW	18	34,445,265 (GRCm39)	missense	probably damaging	1.00
R6354:Apc	UTSW	18	34,445,581 (GRCm39)	missense	probably benign	0.02
R6467:Apc	UTSW	18	34,402,252 (GRCm39)	missense	probably benign	0.22
R6974:Apc	UTSW	18	34,431,480 (GRCm39)	missense	possibly damaging	0.65
R7027:Apc	UTSW	18	34,445,129 (GRCm39)	missense	probably damaging	1.00
R7096:Apc	UTSW	18	34,449,010 (GRCm39)	missense	probably damaging	1.00
R7289:Apc	UTSW	18	34,448,324 (GRCm39)	missense	probably damaging	1.00
R7439:Apc	UTSW	18	34,445,126 (GRCm39)	missense	probably damaging	1.00
R7441:Apc	UTSW	18	34,445,126 (GRCm39)	missense	probably damaging	1.00
R7534:Apc	UTSW	18	34,450,015 (GRCm39)	missense	probably damaging	1.00
R7685:Apc	UTSW	18	34,447,261 (GRCm39)	missense	probably damaging	1.00
R7814:Apc	UTSW	18	34,405,592 (GRCm39)	missense	probably damaging	0.98
R7954:Apc	UTSW	18	34,447,321 (GRCm39)	missense	probably damaging	0.99
R8352:Apc	UTSW	18	34,445,804 (GRCm39)	missense	possibly damaging	0.54
R8452:Apc	UTSW	18	34,445,804 (GRCm39)	missense	possibly damaging	0.54
R8497:Apc	UTSW	18	34,446,083 (GRCm39)	missense	possibly damaging	0.81
R8545:Apc	UTSW	18	34,450,084 (GRCm39)	missense	possibly damaging	0.94
R8554:Apc	UTSW	18	34,445,999 (GRCm39)	missense	probably damaging	1.00
R8955:Apc	UTSW	18	34,401,370 (GRCm39)	missense	probably damaging	1.00
R9014:Apc	UTSW	18	34,354,074 (GRCm39)	start gained	probably benign
R9061:Apc	UTSW	18	34,446,251 (GRCm39)	missense	probably damaging	1.00
R9147:Apc	UTSW	18	34,450,710 (GRCm39)	missense	probably damaging	1.00
R9318:Apc	UTSW	18	34,447,040 (GRCm39)	missense	possibly damaging	0.69
R9521:Apc	UTSW	18	34,445,738 (GRCm39)	missense	probably benign	0.24
R9546:Apc	UTSW	18	34,445,311 (GRCm39)	missense	possibly damaging	0.86
R9547:Apc	UTSW	18	34,445,311 (GRCm39)	missense	possibly damaging	0.86
R9557:Apc	UTSW	18	34,451,412 (GRCm39)	missense	probably damaging	1.00
R9592:Apc	UTSW	18	34,443,823 (GRCm39)	nonsense	probably null
R9675:Apc	UTSW	18	34,449,247 (GRCm39)	missense	probably damaging	1.00
R9736:Apc	UTSW	18	34,450,823 (GRCm39)	missense	probably damaging	1.00
R9792:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
R9793:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
R9795:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
RF046:Apc	UTSW	18	34,415,062 (GRCm39)	critical splice donor site	probably benign
RF063:Apc	UTSW	18	34,415,062 (GRCm39)	critical splice donor site	probably benign
X0021:Apc	UTSW	18	34,445,161 (GRCm39)	missense	probably damaging	1.00
X0025:Apc	UTSW	18	34,445,429 (GRCm39)	missense	probably damaging	1.00
Z1088:Apc	UTSW	18	34,446,220 (GRCm39)	nonsense	probably null
Z1177:Apc	UTSW	18	34,447,516 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CTCAAGAGGCAGGACGATGATTCAC -3'
(R):5'- ATGCCGACTCACTTCTGGGGATAC -3'

Sequencing Primer
(F):5'- CTTCGGAATAGCTCCTCTAGTACAAG -3'
(R):5'- CTGGCATTCTTGGATAAACTTGC -3'

Posted On

2014-05-23