Mutagenetix > Incidental Mutation

Incidental Mutation 'R1741:Wdr95'

200344

Institutional Source

Beutler Lab

Gene Symbol

Wdr95

Ensembl Gene

ENSMUSG00000029658

Gene Name

WD40 repeat domain 95

Synonyms

4930434E21Rik

MMRRC Submission

039773-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R1741 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

149528679-149611894 bp(+) (GRCm38)

Type of Mutation

splice site (5 bp from exon)

DNA Base Change (assembly)

G to A at 149595396 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000144385 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110502] [ENSMUST00000110502] [ENSMUST00000201525] [ENSMUST00000202902] [ENSMUST00000202902]

AlphaFold

D3Z7A8

Predicted Effect

probably null
Transcript: ENSMUST00000110502

SMART Domains

Protein: ENSMUSP00000106128
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
Pfam:WD40	4	28	3.3e-3	PFAM
WD40	32	71	4.38e-5	SMART
WD40	120	159	3.27e-4	SMART
WD40	162	203	1.71e-7	SMART
WD40	206	249	3.57e0	SMART
WD40	263	301	1.7e-2	SMART
Blast:WD40	315	363	3e-14	BLAST
Blast:WD40	367	408	4e-13	BLAST
WD40	421	460	2.01e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000110502

SMART Domains

Protein: ENSMUSP00000106128
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
Pfam:WD40	4	28	3.3e-3	PFAM
WD40	32	71	4.38e-5	SMART
WD40	120	159	3.27e-4	SMART
WD40	162	203	1.71e-7	SMART
WD40	206	249	3.57e0	SMART
WD40	263	301	1.7e-2	SMART
Blast:WD40	315	363	3e-14	BLAST
Blast:WD40	367	408	4e-13	BLAST
WD40	421	460	2.01e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000121108

SMART Domains

Protein: ENSMUSP00000113092
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
Blast:WD40	44	83	9e-11	BLAST
WD40	132	170	1.61e-3	SMART
WD40	174	213	4.38e-5	SMART
WD40	262	301	3.27e-4	SMART
WD40	304	345	1.71e-7	SMART
WD40	348	391	3.57e0	SMART
WD40	405	443	1.7e-2	SMART
Blast:WD40	457	505	3e-14	BLAST
Blast:WD40	509	550	4e-13	BLAST
WD40	563	602	2.01e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000125103

SMART Domains

Protein: ENSMUSP00000120607
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
WD40	104	143	2e-6	SMART
WD40	146	187	1.1e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000201525

SMART Domains

Protein: ENSMUSP00000144234
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
WD40	104	143	2e-6	SMART
WD40	146	187	1.1e-9	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000202902

SMART Domains

Protein: ENSMUSP00000144385
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
Blast:WD40	44	83	9e-11	BLAST
WD40	132	170	1.61e-3	SMART
WD40	174	213	4.38e-5	SMART
WD40	262	301	3.27e-4	SMART
WD40	304	345	1.71e-7	SMART
WD40	348	391	3.57e0	SMART
WD40	405	443	1.7e-2	SMART
Blast:WD40	457	505	3e-14	BLAST
Blast:WD40	509	550	4e-13	BLAST
WD40	563	602	2.01e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000202902

SMART Domains

Protein: ENSMUSP00000144385
Gene: ENSMUSG00000029658

Domain	Start	End	E-Value	Type
Blast:WD40	44	83	9e-11	BLAST
WD40	132	170	1.61e-3	SMART
WD40	174	213	4.38e-5	SMART
WD40	262	301	3.27e-4	SMART
WD40	304	345	1.71e-7	SMART
WD40	348	391	3.57e0	SMART
WD40	405	443	1.7e-2	SMART
Blast:WD40	457	505	3e-14	BLAST
Blast:WD40	509	550	4e-13	BLAST
WD40	563	602	2.01e-4	SMART

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.5%
20x: 92.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034E13Rik	A	G	18: 52,660,506	N37S	probably damaging	Het
9530053A07Rik	G	T	7: 28,157,854	C2209F	probably damaging	Het
Acad10	T	C	5: 121,647,836	K230R	probably damaging	Het
Actl7a	A	G	4: 56,744,252	N260D	probably benign	Het
Adam24	T	C	8: 40,679,603	Y37H	probably benign	Het
Ahcy	G	A	2: 155,064,234	A229V	probably benign	Het
Ap3b2	A	G	7: 81,467,599	V563A	possibly damaging	Het
Bcl9l	T	A	9: 44,509,689	M1427K	probably damaging	Het
Btf3	C	T	13: 98,316,296	M1I	probably null	Het
Btg4	A	T	9: 51,116,610	I27L	probably benign	Het
Ccdc171	A	G	4: 83,620,839	Y366C	probably damaging	Het
Chd3	A	G	11: 69,355,654	Y1085H	probably damaging	Het
Cnot10	T	C	9: 114,597,824	D616G	possibly damaging	Het
Crlf1	C	A	8: 70,500,906	D243E	probably damaging	Het
Cyp2b23	A	G	7: 26,673,077	V371A	possibly damaging	Het
Dennd2a	A	G	6: 39,493,157	S534P	probably damaging	Het
Eln	A	G	5: 134,729,184	V185A	unknown	Het
Epor	C	T	9: 21,959,771	G301D	probably damaging	Het
Fam83f	T	G	15: 80,692,267	V373G	possibly damaging	Het
Fbxl21	A	T	13: 56,537,102	T340S	probably benign	Het
Fez1	T	A	9: 36,843,733	D9E	probably damaging	Het
Fsip2	T	C	2: 82,989,912	F5330L	probably benign	Het
Glis1	G	A	4: 107,568,347	R197Q	probably damaging	Het
Gm10277	TC	T	11: 77,786,002		probably null	Het
Gpr158	A	G	2: 21,827,548	N1153S	probably benign	Het
Gramd4	T	C	15: 86,091,529		probably null	Het
Hhatl	T	A	9: 121,789,059	Y210F	possibly damaging	Het
Hltf	T	C	3: 20,086,188	W422R	probably damaging	Het
Hspa5	T	C	2: 34,772,692	S87P	possibly damaging	Het
Il21	T	C	3: 37,227,662	H111R	probably benign	Het
Ip6k1	G	A	9: 108,040,984	G73S	probably benign	Het
Kdm5b	A	G	1: 134,618,017	D972G	possibly damaging	Het
Kif21b	C	T	1: 136,156,142	A709V	probably damaging	Het
Kmt2d	T	C	15: 98,845,234		probably benign	Het
Lrrc14b	A	G	13: 74,363,586	L125P	probably damaging	Het
Mapk8ip3	A	G	17: 24,899,854	S1169P	probably damaging	Het
Me3	A	T	7: 89,851,833	Y584F	probably damaging	Het
Mxra7	T	G	11: 116,816,244		probably null	Het
Nf1	T	C	11: 79,443,931	S870P	probably benign	Het
Npr2	G	A	4: 43,643,350	G525S	probably damaging	Het
Nyap1	A	T	5: 137,733,125	S726T	probably damaging	Het
Padi4	A	G	4: 140,746,170	V652A	probably damaging	Het
Pclo	A	G	5: 14,676,510		probably benign	Het
Pgm2	G	A	4: 99,964,865		probably null	Het
Piezo2	A	G	18: 63,021,173	S2512P	probably damaging	Het
Ptbp3	A	T	4: 59,482,624	D386E	probably damaging	Het
Ptk2	A	G	15: 73,242,406	V701A	possibly damaging	Het
Ptpn3	A	G	4: 57,254,922	V154A	probably damaging	Het
Rassf4	T	C	6: 116,639,489	E287G	probably damaging	Het
Rnh1	C	T	7: 141,164,023	R174H	probably benign	Het
Scn9a	T	A	2: 66,487,594	I1517F	probably damaging	Het
Sftpb	C	A	6: 72,305,813	A90E	probably benign	Het
Slc39a14	T	C	14: 70,318,744	K61R	probably damaging	Het
Tmem132d	A	C	5: 127,784,858	M733R	probably benign	Het
Tmem248	A	G	5: 130,236,823	I156V	probably benign	Het
Traf2	T	C	2: 25,524,483	D339G	probably damaging	Het
Trappc11	A	G	8: 47,529,327		probably null	Het
Tuba8	T	A	6: 121,222,768	I137N	possibly damaging	Het
Txlnb	A	G	10: 17,838,947	T376A	probably damaging	Het
Usp34	A	G	11: 23,364,103	T663A	probably benign	Het
Vmn2r26	T	A	6: 124,061,472	F669I	probably damaging	Het
Wfdc8	T	G	2: 164,608,869		probably benign	Het
Zfp64	A	C	2: 168,926,318	V458G	probably benign	Het
Zfp868	C	T	8: 69,611,868	G272D	probably damaging	Het

Other mutations in Wdr95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00429:Wdr95	APN	5	149595244	critical splice acceptor site	probably benign	0.00
IGL02352:Wdr95	APN	5	149580619	missense	probably damaging	0.99
IGL02359:Wdr95	APN	5	149580619	missense	probably damaging	0.99
IGL02478:Wdr95	APN	5	149596321	missense	probably benign	0.02
IGL03078:Wdr95	APN	5	149611597	missense	possibly damaging	0.63
IGL03201:Wdr95	APN	5	149581887	splice site	probably null
P0037:Wdr95	UTSW	5	149588071	missense	probably benign	0.27
R0115:Wdr95	UTSW	5	149564390	missense	probably damaging	1.00
R0538:Wdr95	UTSW	5	149580806	missense	probably damaging	1.00
R0606:Wdr95	UTSW	5	149588130	missense	probably damaging	1.00
R0723:Wdr95	UTSW	5	149574048	missense	probably damaging	1.00
R1104:Wdr95	UTSW	5	149606337	missense	probably benign	0.00
R1233:Wdr95	UTSW	5	149581858	missense	possibly damaging	0.61
R1233:Wdr95	UTSW	5	149595364	missense	probably benign	0.00
R1344:Wdr95	UTSW	5	149588098	missense	probably damaging	1.00
R1513:Wdr95	UTSW	5	149599294	missense	probably benign	0.00
R1623:Wdr95	UTSW	5	149574116	missense	probably damaging	1.00
R1633:Wdr95	UTSW	5	149593172	missense	probably damaging	0.98
R1664:Wdr95	UTSW	5	149595287	missense	probably damaging	0.98
R1686:Wdr95	UTSW	5	149593101	missense	probably damaging	1.00
R1750:Wdr95	UTSW	5	149581886	splice site	probably null
R1774:Wdr95	UTSW	5	149564392	nonsense	probably null
R1831:Wdr95	UTSW	5	149552426	missense	probably damaging	1.00
R1838:Wdr95	UTSW	5	149599366	missense	probably benign	0.00
R1907:Wdr95	UTSW	5	149552426	missense	probably damaging	1.00
R2019:Wdr95	UTSW	5	149574148	splice site	probably benign
R2063:Wdr95	UTSW	5	149579162	splice site	probably null
R2392:Wdr95	UTSW	5	149580670	missense	probably benign	0.03
R2863:Wdr95	UTSW	5	149581856	nonsense	probably null
R4116:Wdr95	UTSW	5	149597575	missense	probably benign	0.02
R4237:Wdr95	UTSW	5	149563337	nonsense	probably null
R4420:Wdr95	UTSW	5	149532666	missense	probably damaging	0.99
R4639:Wdr95	UTSW	5	149581814	splice site	probably benign
R4824:Wdr95	UTSW	5	149595332	missense	probably damaging	1.00
R4911:Wdr95	UTSW	5	149611692	nonsense	probably null
R5016:Wdr95	UTSW	5	149544801	missense	probably benign	0.00
R5458:Wdr95	UTSW	5	149564414	missense	probably damaging	1.00
R5486:Wdr95	UTSW	5	149596330	nonsense	probably null
R5613:Wdr95	UTSW	5	149584470	missense	probably damaging	1.00
R5906:Wdr95	UTSW	5	149564227	missense	possibly damaging	0.50
R5956:Wdr95	UTSW	5	149594482	missense	probably benign	0.00
R6309:Wdr95	UTSW	5	149580803	critical splice acceptor site	probably null
R6867:Wdr95	UTSW	5	149580923	splice site	probably null
R6964:Wdr95	UTSW	5	149581850	missense	probably damaging	1.00
R7008:Wdr95	UTSW	5	149611540	missense	probably benign	0.00
R7208:Wdr95	UTSW	5	149595371	missense	probably benign	0.02
R7309:Wdr95	UTSW	5	149606293	missense	probably benign	0.01
R7504:Wdr95	UTSW	5	149581846	missense	probably damaging	0.99
R7660:Wdr95	UTSW	5	149594480	missense	possibly damaging	0.86
R7997:Wdr95	UTSW	5	149579157	critical splice donor site	probably null
R8084:Wdr95	UTSW	5	149588133	missense	probably damaging	1.00
R8356:Wdr95	UTSW	5	149579107	missense	probably damaging	1.00
R8456:Wdr95	UTSW	5	149579107	missense	probably damaging	1.00
R8523:Wdr95	UTSW	5	149573996	missense	probably damaging	1.00
R8682:Wdr95	UTSW	5	149595287	missense	possibly damaging	0.94
R9451:Wdr95	UTSW	5	149580700	missense	probably benign	0.01
R9453:Wdr95	UTSW	5	149552452	missense	probably damaging	0.99
R9779:Wdr95	UTSW	5	149581828	missense	probably benign	0.01
X0024:Wdr95	UTSW	5	149588167	missense	possibly damaging	0.81
Z1176:Wdr95	UTSW	5	149566436	missense	probably benign	0.34
Z1177:Wdr95	UTSW	5	149544776	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TCTCAGTCCAGAGAGAAAGCCCAG -3'
(R):5'- GCATTAGACACAAGTTGTCAATGTGCC -3'

Sequencing Primer
(F):5'- AGGTGAGCGGCATCGTG -3'
(R):5'- TGGTGCTAACAGATGCCC -3'

Posted On

2014-05-23