Mutagenetix > Incidental Mutation

Incidental Mutation 'R1411:Vmn1r199'

200818

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r199

Ensembl Gene

ENSMUSG00000069292

Gene Name

vomeronasal 1 receptor 199

Synonyms

V1rh4

MMRRC Submission

039467-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R1411 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22566708-22567811 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22567671 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 322 (F322L)

Ref Sequence

ENSEMBL: ENSMUSP00000153918 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091732] [ENSMUST00000227685] [ENSMUST00000227689] [ENSMUST00000227846]

AlphaFold

Q8R280

Predicted Effect

probably benign
Transcript: ENSMUST00000091732
AA Change: F322L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000089326
Gene: ENSMUSG00000069292
AA Change: F322L

Domain	Start	End	E-Value	Type
low complexity region	26	37	N/A	INTRINSIC
Pfam:TAS2R	43	338	3.3e-7	PFAM
Pfam:V1R	75	340	1.6e-33	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000120663

Predicted Effect

probably benign
Transcript: ENSMUST00000227685
AA Change: F322L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

Predicted Effect

probably benign
Transcript: ENSMUST00000227689
AA Change: F279L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000227846
AA Change: F279L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.3%
20x: 92.5%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd8	T	A	8: 71,914,374 (GRCm39)	I85F	probably damaging	Het
Acp4	A	G	7: 43,906,267 (GRCm39)		probably benign	Het
Baz1b	T	C	5: 135,259,177 (GRCm39)	F1080L	possibly damaging	Het
Cbx5	A	T	15: 103,121,547 (GRCm39)	M30K	probably benign	Het
Cdc73	A	G	1: 143,485,252 (GRCm39)		probably benign	Het
Cdcp1	A	T	9: 123,019,177 (GRCm39)	L34Q	probably damaging	Het
Chd8	C	T	14: 52,462,103 (GRCm39)	V738I	probably benign	Het
Cpeb2	T	G	5: 43,391,113 (GRCm39)		probably benign	Het
Cyp2c67	T	A	19: 39,627,035 (GRCm39)	D265V	probably damaging	Het
Cyp2j11	A	G	4: 96,233,453 (GRCm39)	I81T	probably benign	Het
Dbx2	T	C	15: 95,530,262 (GRCm39)	E235G	probably damaging	Het
Dgkq	C	A	5: 108,798,228 (GRCm39)	V677F	probably damaging	Het
Ercc5	A	G	1: 44,217,441 (GRCm39)	N928S	probably damaging	Het
Flt1	C	T	5: 147,517,126 (GRCm39)	V1054M	probably damaging	Het
Flywch1	T	C	17: 23,974,798 (GRCm39)	D614G	probably damaging	Het
Frmd3	T	C	4: 74,071,858 (GRCm39)	F247L	probably damaging	Het
Gm1527	A	G	3: 28,968,632 (GRCm39)	N228S	probably benign	Het
Gpld1	T	A	13: 25,146,791 (GRCm39)	L251Q	probably damaging	Het
Gvin-ps3	C	T	7: 105,682,686 (GRCm39)	A190T	probably benign	Het
Gzma	C	T	13: 113,232,742 (GRCm39)	V117I	probably benign	Het
Hydin	C	A	8: 111,301,663 (GRCm39)	T3798K	probably benign	Het
Il1rapl1	T	A	X: 85,790,904 (GRCm39)	S679C	possibly damaging	Het
Lrrc8c	G	A	5: 105,756,045 (GRCm39)	A607T	probably damaging	Het
Ltbp1	A	G	17: 75,532,280 (GRCm39)	Q118R	possibly damaging	Het
Mfsd4b4	A	T	10: 39,768,136 (GRCm39)	M319K	probably damaging	Het
Mroh2b	A	C	15: 4,947,799 (GRCm39)	H538P	probably damaging	Het
Nrbp1	T	A	5: 31,403,157 (GRCm39)	I210N	probably damaging	Het
Nup188	A	G	2: 30,233,807 (GRCm39)	T1733A	probably benign	Het
Nup58	A	T	14: 60,482,119 (GRCm39)		probably benign	Het
Ofcc1	A	G	13: 40,296,263 (GRCm39)	S524P	probably benign	Het
Or6x1	C	T	9: 40,098,435 (GRCm39)	T8I	possibly damaging	Het
Padi4	C	T	4: 140,479,914 (GRCm39)	S413N	probably damaging	Het
Pdzrn4	A	G	15: 92,668,894 (GRCm39)	*1015W	probably null	Het
Pkhd1	G	A	1: 20,444,120 (GRCm39)	P2314L	probably damaging	Het
Serpinb9c	C	G	13: 33,335,817 (GRCm39)	V212L	probably benign	Het
Slc25a23	A	G	17: 57,366,622 (GRCm39)	F18L	probably damaging	Het
Smarca4	C	A	9: 21,570,251 (GRCm39)	N751K	probably damaging	Het
Tfip11	G	T	5: 112,480,899 (GRCm39)	V292L	probably benign	Het
Tnrc18	T	C	5: 142,751,702 (GRCm39)	K1201R	unknown	Het
Vmn1r201	T	C	13: 22,658,849 (GRCm39)	V21A	probably benign	Het
Vmn2r108	A	T	17: 20,683,107 (GRCm39)	I699K	probably damaging	Het
Vmn2r117	T	C	17: 23,679,527 (GRCm39)	N566D	probably damaging	Het
Wdr12	A	T	1: 60,127,231 (GRCm39)	D141E	probably benign	Het
Zfp974	A	G	7: 27,610,634 (GRCm39)	S364P	probably benign	Het

Other mutations in Vmn1r199

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00930:Vmn1r199	APN	13	22,567,029 (GRCm39)	missense	possibly damaging	0.64
IGL01959:Vmn1r199	APN	13	22,567,290 (GRCm39)	missense	probably benign	0.07
IGL02270:Vmn1r199	APN	13	22,567,176 (GRCm39)	missense	probably damaging	1.00
IGL02687:Vmn1r199	APN	13	22,566,961 (GRCm39)	missense	possibly damaging	0.91
IGL03143:Vmn1r199	APN	13	22,567,299 (GRCm39)	missense	probably damaging	1.00
R0528:Vmn1r199	UTSW	13	22,566,736 (GRCm39)	missense	probably benign
R2075:Vmn1r199	UTSW	13	22,567,435 (GRCm39)	missense	probably damaging	1.00
R5057:Vmn1r199	UTSW	13	22,567,575 (GRCm39)	missense	possibly damaging	0.93
R5824:Vmn1r199	UTSW	13	22,567,748 (GRCm39)	missense	probably benign
R5977:Vmn1r199	UTSW	13	22,567,416 (GRCm39)	missense	probably benign	0.08
R6019:Vmn1r199	UTSW	13	22,566,769 (GRCm39)	missense	possibly damaging	0.85
R6419:Vmn1r199	UTSW	13	22,567,777 (GRCm39)	missense	possibly damaging	0.66
R7624:Vmn1r199	UTSW	13	22,566,906 (GRCm39)	missense	probably benign	0.00
R7637:Vmn1r199	UTSW	13	22,566,845 (GRCm39)	missense	probably benign	0.05
R8505:Vmn1r199	UTSW	13	22,567,317 (GRCm39)	missense	probably benign	0.01
R8692:Vmn1r199	UTSW	13	22,567,809 (GRCm39)	makesense	probably null
R8735:Vmn1r199	UTSW	13	22,567,537 (GRCm39)	missense	probably damaging	1.00
R8791:Vmn1r199	UTSW	13	22,567,687 (GRCm39)	missense	probably damaging	1.00
R9054:Vmn1r199	UTSW	13	22,567,724 (GRCm39)	missense	possibly damaging	0.95
R9258:Vmn1r199	UTSW	13	22,566,822 (GRCm39)	missense	possibly damaging	0.53
R9586:Vmn1r199	UTSW	13	22,567,716 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCCAAAGTCTGCATGGCACATC -3'
(R):5'- ACCTCAGTGCAAGGCTTGAATAACC -3'

Sequencing Primer
(F):5'- CATGGTCCTGAGAGATGCAGTC -3'
(R):5'- CAAGGCTTGAATAACCAAGATTATTG -3'

Posted On

2014-05-23