Mutagenetix > Incidental Mutation

Incidental Mutation 'R1185:Fam162b'

200864

Institutional Source

Beutler Lab

Gene Symbol

Fam162b

Ensembl Gene

ENSMUSG00000019909

Gene Name

family with sequence similarity 162, member B

Synonyms

9430073N08Rik

MMRRC Submission

039257-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R1185 (G1)

Quality Score

189

Status

Validated

Chromosome

Chromosomal Location

51461512-51466613 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 51466439 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 27 (W27R)

Ref Sequence

ENSEMBL: ENSMUSP00000020064 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020064]

AlphaFold

Q9CX19

Predicted Effect

probably benign
Transcript: ENSMUST00000020064
AA Change: W27R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000020064
Gene: ENSMUSG00000019909
AA Change: W27R

Domain	Start	End	E-Value	Type
Pfam:DUF1075	15	154	3.8e-56	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ac165356.1	C	T	7: 105,682,686 (GRCm39)	A190T	probably benign	Het
Aimp2	A	G	5: 143,841,509 (GRCm39)	S110P	possibly damaging	Het
Akap9	A	G	5: 3,998,783 (GRCm39)	T51A	probably benign	Het
Arhgef25	A	G	10: 127,019,650 (GRCm39)	F430L	possibly damaging	Het
Brap	T	C	5: 121,813,342 (GRCm39)	V235A	probably damaging	Het
Cd69	C	T	6: 129,247,148 (GRCm39)	G23D	probably damaging	Het
Cecr2	C	T	6: 120,735,166 (GRCm39)	R24*	probably null	Het
Celsr2	T	C	3: 108,307,025 (GRCm39)	D1974G	possibly damaging	Het
Cps1	A	G	1: 67,234,358 (GRCm39)	K915R	probably benign	Het
Csmd1	T	C	8: 16,408,362 (GRCm39)	D401G	probably damaging	Het
Dusp13b	A	G	14: 21,785,086 (GRCm39)	F141S	probably damaging	Het
Eif1ad19	A	G	12: 87,740,478 (GRCm39)	V27A	probably benign	Het
Focad	A	G	4: 88,096,424 (GRCm39)	T269A	probably benign	Het
Ghr	T	A	15: 3,357,544 (GRCm39)	R241S	possibly damaging	Het
Hirip3	AAGAG	AAG	7: 126,462,832 (GRCm39)		probably null	Het
Ift70a1	A	T	2: 75,810,696 (GRCm39)	N462K	probably damaging	Het
Itgb2l	A	G	16: 96,230,240 (GRCm39)	Y357H	possibly damaging	Het
Jrkl	T	C	9: 13,244,938 (GRCm39)	D241G	possibly damaging	Het
Lmod1	A	G	1: 135,291,967 (GRCm39)	D274G	probably benign	Het
Lrrn2	A	G	1: 132,866,959 (GRCm39)	S675G	probably benign	Het
Ltbp4	G	C	7: 27,009,960 (GRCm39)	P1200R	probably damaging	Het
Mdn1	T	C	4: 32,735,576 (GRCm39)	L3414P	possibly damaging	Het
Muc19	A	G	15: 91,762,743 (GRCm39)		noncoding transcript	Het
Myo16	T	A	8: 10,683,624 (GRCm39)	S1856T	probably damaging	Het
Neb	A	G	2: 52,186,310 (GRCm39)	Y921H	probably damaging	Het
Or2n1c	T	A	17: 38,520,074 (GRCm39)	*313R	probably null	Het
Pgap3	T	C	11: 98,281,960 (GRCm39)	D117G	probably damaging	Het
Ppp1r9b	T	C	11: 94,892,812 (GRCm39)	F671L	possibly damaging	Het
Purg	T	G	8: 33,876,897 (GRCm39)	Y178*	probably null	Het
Rsph10b	G	A	5: 143,903,280 (GRCm39)		probably benign	Het
Sorbs1	T	A	19: 40,371,050 (GRCm39)	D79V	probably damaging	Het
Tcaf3	T	C	6: 42,568,368 (GRCm39)	T663A	probably damaging	Het
Timd4	C	A	11: 46,708,475 (GRCm39)	T167K	probably damaging	Het
Tjp2	A	G	19: 24,108,527 (GRCm39)	L195P	possibly damaging	Het
Tnr	G	A	1: 159,679,856 (GRCm39)	A277T	probably benign	Het
Trpm3	G	A	19: 22,891,781 (GRCm39)		probably benign	Het
Unc13a	C	T	8: 72,114,477 (GRCm39)	G181D	probably benign	Het
Vmn1r11	T	A	6: 57,114,492 (GRCm39)	L52Q	possibly damaging	Het
Zfp27	T	C	7: 29,595,254 (GRCm39)	D237G	possibly damaging	Het
Zfp39	T	C	11: 58,793,670 (GRCm39)	T23A	possibly damaging	Het
Zfp459	T	G	13: 67,556,600 (GRCm39)	N161T	probably benign	Het

Other mutations in Fam162b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00567:Fam162b	APN	10	51,466,390 (GRCm39)	missense	possibly damaging	0.94
IGL02948:Fam162b	APN	10	51,463,392 (GRCm39)	missense	probably damaging	1.00
R0709:Fam162b	UTSW	10	51,463,347 (GRCm39)	missense	probably damaging	1.00
R1185:Fam162b	UTSW	10	51,466,439 (GRCm39)	missense	probably benign
R1185:Fam162b	UTSW	10	51,466,439 (GRCm39)	missense	probably benign
R1505:Fam162b	UTSW	10	51,463,298 (GRCm39)	missense	probably damaging	1.00
R1735:Fam162b	UTSW	10	51,463,307 (GRCm39)	missense	probably damaging	1.00
R1961:Fam162b	UTSW	10	51,466,430 (GRCm39)	missense	probably benign	0.00
R2401:Fam162b	UTSW	10	51,463,314 (GRCm39)	missense	probably damaging	0.99
R6059:Fam162b	UTSW	10	51,466,403 (GRCm39)	missense	probably benign	0.28
R6196:Fam162b	UTSW	10	51,463,506 (GRCm39)	splice site	probably null
R6284:Fam162b	UTSW	10	51,461,598 (GRCm39)	missense	probably damaging	0.99
R6625:Fam162b	UTSW	10	51,466,391 (GRCm39)	missense	probably damaging	1.00
R7324:Fam162b	UTSW	10	51,466,282 (GRCm39)	splice site	probably null
R7380:Fam162b	UTSW	10	51,466,572 (GRCm39)	start gained	probably benign
R8945:Fam162b	UTSW	10	51,466,469 (GRCm39)	missense	probably benign	0.02
R9415:Fam162b	UTSW	10	51,466,155 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCCACTTCACACTTACGGTACAAGG -3'
(R):5'- ATGCAGCTCAGCATCCAGGATCAG -3'

Sequencing Primer
(F):5'- TAGATTTGAAACGCCCGGTC -3'
(R):5'- TCCAGGATCAGGAGGAGGC -3'

Posted On

2014-05-23