Mutagenetix > Incidental Mutation

Incidental Mutation 'R1464:Helz2'

200919

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

039518-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1464 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 181239654 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 345 (E345G)

Ref Sequence

ENSEMBL: ENSMUSP00000091756 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094203
AA Change: E345G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: E345G

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108831
AA Change: E345G

PolyPhen 2 Score 0.228 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: E345G

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121484
AA Change: E345G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: E345G

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149417

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155049

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.0%
20x: 91.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930430A15Rik	A	G	2: 111,225,403		probably null	Het
6030469F06Rik	A	G	12: 31,184,915		noncoding transcript	Het
Abca2	T	G	2: 25,447,834		probably benign	Het
Adamts8	T	A	9: 30,951,377	W86R	probably benign	Het
Adh1	A	G	3: 138,288,747		probably null	Het
Adipor2	A	T	6: 119,361,843	W150R	probably damaging	Het
Aff4	T	A	11: 53,372,524	S124T	probably damaging	Het
Ahnak	G	T	19: 9,004,896	K1181N	probably damaging	Het
Ak2	C	A	4: 129,002,359		probably benign	Het
Alkbh5	A	G	11: 60,539,047	I209V	probably benign	Het
Ankrd11	T	C	8: 122,892,724	E1442G	probably damaging	Het
Apba2	T	A	7: 64,695,549	D162E	probably benign	Het
Asic3	G	A	5: 24,413,821	G37E	probably damaging	Het
AU040320	A	T	4: 126,792,031	K133N	possibly damaging	Het
C1qtnf7	A	G	5: 43,609,139	S34G	probably benign	Het
Carf	A	G	1: 60,125,906		probably benign	Het
Ccdc148	T	A	2: 58,906,362	R463*	probably null	Het
Ccdc148	T	C	2: 58,934,443	R329G	probably damaging	Het
Ccdc185	A	G	1: 182,748,698	I142T	probably benign	Het
Cd274	T	C	19: 29,382,592		probably benign	Het
Chrna10	G	A	7: 102,114,247	P114S	probably damaging	Het
Chst10	A	G	1: 38,865,691	I311T	probably damaging	Het
Cpne9	T	C	6: 113,294,737	Y353H	probably damaging	Het
Cubn	G	T	2: 13,325,288	A2594E	possibly damaging	Het
Cyp3a13	T	C	5: 137,905,565	N277S	possibly damaging	Het
Dctn4	A	G	18: 60,538,406	T117A	probably damaging	Het
Ddah1	A	T	3: 145,853,274	K96*	probably null	Het
Ddx5	A	C	11: 106,784,885	D326E	probably benign	Het
Dlg2	T	C	7: 91,968,198	S323P	probably damaging	Het
Dnaaf1	C	T	8: 119,579,310	H109Y	probably damaging	Het
Dnah8	C	A	17: 30,695,173	R1098S	possibly damaging	Het
Dnajc13	T	C	9: 104,214,167	T642A	probably benign	Het
Dnajc18	A	G	18: 35,680,847	S290P	possibly damaging	Het
Dscam	T	A	16: 96,801,253	H663L	possibly damaging	Het
Eefsec	C	T	6: 88,376,200		probably benign	Het
Emc1	A	G	4: 139,370,937	N740S	probably damaging	Het
Enpp2	C	T	15: 54,863,812	G541D	probably damaging	Het
Fam234b	C	T	6: 135,228,492	T485I	probably benign	Het
Fbxl13	A	T	5: 21,483,991	I773K	probably benign	Het
Fmo4	A	G	1: 162,794,355	F429S	possibly damaging	Het
Fndc3b	A	G	3: 27,440,185		probably benign	Het
Frem1	T	C	4: 83,011,879	S277G	probably damaging	Het
Gaa	A	G	11: 119,272,984	I221V	probably benign	Het
Gm12794	T	A	4: 101,941,306	L158Q	probably damaging	Het
Gm6614	C	T	6: 141,992,517	W225*	probably null	Het
Gnptab	A	G	10: 88,445,754		probably benign	Het
Gphn	T	A	12: 78,612,964		probably benign	Het
H2afy	A	T	13: 56,083,136	S310T	probably damaging	Het
Ifna4	G	T	4: 88,842,000	R47I	probably damaging	Het
Igsf21	C	A	4: 140,034,525	A281S	probably benign	Het
Ikzf3	T	A	11: 98,516,905	I37L	probably benign	Het
Inpp5d	A	T	1: 87,698,105		probably benign	Het
Jag1	T	C	2: 137,115,648	E48G	probably damaging	Het
Jarid2	G	T	13: 44,848,381	V57F	probably damaging	Het
Kcnj4	A	G	15: 79,485,404	L125P	probably damaging	Het
Kif21b	A	G	1: 136,156,153	K713E	possibly damaging	Het
Layn	T	A	9: 51,057,586	S286C	probably damaging	Het
Lepr	T	A	4: 101,735,681	D164E	probably benign	Het
Map3k1	T	C	13: 111,755,871	H950R	possibly damaging	Het
Map4k5	C	A	12: 69,805,350	V801L	possibly damaging	Het
Mapkbp1	T	A	2: 120,021,261	S895T	probably benign	Het
Mthfr	T	A	4: 148,053,572		probably benign	Het
Naca	T	A	10: 128,048,288	M2157K	probably damaging	Het
Nav2	T	C	7: 49,362,204	I61T	probably damaging	Het
Nkx2-5	C	A	17: 26,839,279	A234S	probably benign	Het
Nol8	G	A	13: 49,676,788	S1116N	probably benign	Het
Nphp3	T	C	9: 104,031,879		probably benign	Het
Nppa	T	C	4: 148,000,847	S5P	probably benign	Het
Nup210	A	G	6: 91,053,569	V123A	possibly damaging	Het
Olfr532	G	T	7: 140,419,373	N133K	probably benign	Het
Osbpl11	A	G	16: 33,229,085	K604R	probably damaging	Het
Osbpl1a	C	T	18: 12,914,558	S113N	probably benign	Het
P2rx4	C	A	5: 122,714,539	P92Q	probably damaging	Het
Pde9a	A	T	17: 31,473,162	Q148L	probably benign	Het
Phf21b	T	A	15: 84,804,959	H122L	probably damaging	Het
Pik3ca	T	A	3: 32,461,841	F977I	probably damaging	Het
Pkd1l3	A	G	8: 109,636,427		probably benign	Het
Pp2d1	T	G	17: 53,515,987	K17T	possibly damaging	Het
Ppp4r2	A	G	6: 100,866,566	E415G	probably damaging	Het
Prkg1	A	G	19: 30,578,870	S559P	probably damaging	Het
Prss45	A	T	9: 110,840,951	Y276F	possibly damaging	Het
Ptk7	G	T	17: 46,572,591	N849K	probably damaging	Het
Rnf121	A	G	7: 102,031,575	I125T	possibly damaging	Het
Rnf17	A	G	14: 56,461,911	N502S	probably damaging	Het
Sap25	T	A	5: 137,642,360	Y167*	probably null	Het
Sdk2	G	A	11: 113,830,080	T1341I	possibly damaging	Het
Sgcb	A	G	5: 73,635,553	V302A	probably benign	Het
Skor1	T	A	9: 63,140,111	M865L	possibly damaging	Het
Slc25a45	T	C	19: 5,879,900		probably benign	Het
Slc2a3	A	G	6: 122,737,310		probably benign	Het
Slc35g1	C	A	19: 38,403,217	L316I	probably benign	Het
Spint4	T	A	2: 164,698,648	L33H	probably damaging	Het
Sptlc3	A	T	2: 139,547,234	D178V	probably benign	Het
Stoml1	A	G	9: 58,260,426		probably benign	Het
Tbc1d2	T	A	4: 46,606,491	Y818F	possibly damaging	Het
Tbc1d24	A	T	17: 24,181,223		probably null	Het
Teddm2	C	T	1: 153,850,531	W146*	probably null	Het
Ttn	T	A	2: 76,758,994	D12948V	probably damaging	Het
Txndc8	T	C	4: 58,000,274	T102A	probably damaging	Het
Uxs1	G	A	1: 43,764,916	Q280*	probably null	Het
Vmn1r167	T	C	7: 23,505,256	T112A	possibly damaging	Het
Vmn1r195	A	T	13: 22,279,178	I273L	probably benign	Het
Vmn1r28	T	A	6: 58,265,232	M20K	probably benign	Het
Vmn1r53	G	A	6: 90,223,932	L137F	probably benign	Het
Vmn2r105	G	A	17: 20,228,742		probably benign	Het
Vps13b	C	T	15: 35,709,484	A1859V	probably benign	Het
Vwa5b2	A	G	16: 20,596,269	H347R	probably benign	Het
Wnk2	G	A	13: 49,081,975	P655S	probably damaging	Het
Zan	T	G	5: 137,419,929	D2969A	unknown	Het
Zbtb24	A	G	10: 41,455,079	H334R	probably damaging	Het
Zfp108	A	G	7: 24,260,548	D188G	probably benign	Het
Zfp784	A	T	7: 5,035,801	C253S	possibly damaging	Het
Zfr	T	G	15: 12,146,372	C336W	probably damaging	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181229702	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181233006	nonsense	probably null
IGL00704:Helz2	APN	2	181234385	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181232245	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181233977	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181232881	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181238481	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181232185	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181231690	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181230911	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181231022	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181235026	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181231146	intron	probably benign
IGL03003:Helz2	APN	2	181240253	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181229222	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181231804	missense	probably benign	0.00
Colby	UTSW	2	181233202	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181234834	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0013:Helz2	UTSW	2	181240959	missense	probably benign
R0014:Helz2	UTSW	2	181240511	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181240511	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181228821	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181228821	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181236407	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181236407	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181237802	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181232269	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181230430	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181230430	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181230593	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181232209	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181229656	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181227770	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181234825	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181232089	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181232116	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181240881	missense	probably benign
R0826:Helz2	UTSW	2	181240853	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181230777	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181236135	missense	probably benign
R1170:Helz2	UTSW	2	181229815	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181231128	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181237596	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181232981	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181235524	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181239654	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181232804	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181233228	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181234147	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181236263	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181234987	missense	probably benign
R1779:Helz2	UTSW	2	181238459	missense	possibly damaging	0.84
R1837:Helz2	UTSW	2	181229289	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181232085	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181234289	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181233750	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	181231329	missense	probably benign	0.45
R2034:Helz2	UTSW	2	181232578	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181237479	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181240544	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181235102	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181231380	missense	probably benign
R2180:Helz2	UTSW	2	181233732	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181229048	nonsense	probably null
R2248:Helz2	UTSW	2	181233433	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181232912	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181240742	missense	probably benign
R3617:Helz2	UTSW	2	181233061	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181240389	nonsense	probably null
R3803:Helz2	UTSW	2	181239996	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181229710	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181240475	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181229902	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181228833	missense	probably benign
R4624:Helz2	UTSW	2	181239308	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181238417	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181237417	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181230120	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181236147	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181232438	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181240916	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181240569	missense	probably benign
R5089:Helz2	UTSW	2	181235149	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181230757	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181234846	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181235528	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	181235069	missense	probably benign
R5559:Helz2	UTSW	2	181230126	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181240258	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181237289	intron	probably benign
R5805:Helz2	UTSW	2	181240508	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181236396	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181232656	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181234028	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181230384	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181230767	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181231050	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181240313	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181233038	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181232294	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181235945	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181233202	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181235069	missense	probably benign
R6581:Helz2	UTSW	2	181229379	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181239557	nonsense	probably null
R6964:Helz2	UTSW	2	181230428	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181240514	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181231285	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181238423	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181230854	missense	probably benign	0.00
R7512:Helz2	UTSW	2	181235600	splice site	probably null
R7583:Helz2	UTSW	2	181237572	missense	probably benign	0.06
R7724:Helz2	UTSW	2	181231996	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181230355	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181234531	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181233991	missense	probably benign
R7799:Helz2	UTSW	2	181237989	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181232902	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181237750	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181240205	missense	probably benign	0.34
R8030:Helz2	UTSW	2	181237896	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	181238262	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181229331	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181238102	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181230157	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181229331	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181229557	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181232767	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181233127	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181238380	missense
R8953:Helz2	UTSW	2	181233091	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181229614	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181237788	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181234693	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181228999	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181232468	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181240175	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181239640	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181240055	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181230090	missense	probably benign
R9186:Helz2	UTSW	2	181234664	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181240948	missense	probably benign
R9407:Helz2	UTSW	2	181240182	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181232917	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181236452	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181240221	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181240677	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181240232	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181240823	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181231741	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181237564	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181235961	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAGTGACAGACACTCCACGGATG -3'
(R):5'- CCAAGGGATTGTGAGGTCCACAAG -3'

Sequencing Primer
(F):5'- AGAAGTTGGGCTTTCAGCAC -3'
(R):5'- GTGCCCTCTGCATGTCAAG -3'

Posted On

2014-05-23