Mutagenetix > Incidental Mutation

Incidental Mutation 'R0070:Or2h15'

201470

Institutional Source

Beutler Lab

Gene Symbol

Or2h15

Ensembl Gene

ENSMUSG00000067186

Gene Name

olfactory receptor family 2 subfamily H member 15

Synonyms

Olfr132, MOR256-49, GA_x6K02T2PSCP-2579687-2578746

MMRRC Submission

038361-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.158) question?

Stock #

R0070 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

38441140-38442081 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 38441780 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 101 (L101P)

Ref Sequence

ENSEMBL: ENSMUSP00000149593 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087128] [ENSMUST00000216804]

AlphaFold

Q7TRI8

Predicted Effect

probably damaging
Transcript: ENSMUST00000087128
AA Change: L101P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000084368
Gene: ENSMUSG00000067186
AA Change: L101P

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srv	25	244	3.1e-6	PFAM
Pfam:7tm_4	31	308	4.6e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.9e-7	PFAM
Pfam:7tm_1	41	290	8.1e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000216804
AA Change: L101P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217361

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.9%
10x: 97.7%
20x: 96.1%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgre4	T	C	17: 56,109,154 (GRCm39)	I387T	probably damaging	Het
Alpi	A	G	1: 87,028,881 (GRCm39)		probably benign	Het
Ankfn1	A	T	11: 89,283,128 (GRCm39)	L173Q	probably damaging	Het
Atp2a1	T	C	7: 126,046,624 (GRCm39)	E892G	probably benign	Het
AU018091	T	C	7: 3,208,738 (GRCm39)		probably null	Het
Capn12	T	C	7: 28,588,551 (GRCm39)		probably benign	Het
Capn2	C	A	1: 182,301,434 (GRCm39)		probably benign	Het
Cd79b	A	G	11: 106,202,744 (GRCm39)		probably benign	Het
Cdh20	C	T	1: 110,026,102 (GRCm39)	A446V	probably benign	Het
Ciapin1	T	C	8: 95,551,847 (GRCm39)	N246S	possibly damaging	Het
Cmip	T	A	8: 118,153,293 (GRCm39)	I270N	probably damaging	Het
Cyp2d40	A	G	15: 82,644,975 (GRCm39)	V225A	unknown	Het
Dnah9	A	G	11: 66,050,866 (GRCm39)	V142A	probably benign	Het
Dnai4	A	T	4: 102,917,131 (GRCm39)	I571K	probably damaging	Het
Flt3	A	G	5: 147,309,536 (GRCm39)		probably benign	Het
Gm10238	A	G	15: 75,109,434 (GRCm39)		noncoding transcript	Het
Gm4787	T	A	12: 81,425,840 (GRCm39)	D106V	probably damaging	Het
Hipk2	G	A	6: 38,795,919 (GRCm39)	R117*	probably null	Het
Hycc1	T	C	5: 24,169,997 (GRCm39)	S451G	probably damaging	Het
Ifna11	A	G	4: 88,738,512 (GRCm39)	D106G	possibly damaging	Het
Igkv1-115	G	A	6: 68,138,402 (GRCm39)	V2I	probably benign	Het
Itga6	T	C	2: 71,657,060 (GRCm39)		probably benign	Het
Kcnj6	C	A	16: 94,742,056 (GRCm39)	K5N	probably benign	Het
Kcnt1	T	C	2: 25,782,374 (GRCm39)	V191A	probably benign	Het
Lcorl	G	A	5: 45,891,043 (GRCm39)	R437C	probably damaging	Het
Man2a1	G	A	17: 64,966,074 (GRCm39)		probably null	Het
Map3k14	T	A	11: 103,130,380 (GRCm39)		probably null	Het
Mtch1	T	A	17: 29,559,033 (GRCm39)		probably benign	Het
Myo1c	A	G	11: 75,551,076 (GRCm39)	N217S	probably benign	Het
Or2w4	T	C	13: 21,795,431 (GRCm39)	K236R	possibly damaging	Het
Orm3	A	G	4: 63,274,883 (GRCm39)	T64A	probably benign	Het
Phf20l1	T	G	15: 66,511,840 (GRCm39)	W940G	probably damaging	Het
Phldb1	C	T	9: 44,619,201 (GRCm39)	R844H	probably damaging	Het
Piezo2	T	C	18: 63,235,155 (GRCm39)	D814G	probably damaging	Het
Pkd2	T	C	5: 104,614,856 (GRCm39)	C233R	probably damaging	Het
Prkd3	A	G	17: 79,261,939 (GRCm39)	Y792H	probably damaging	Het
Pth1r	A	T	9: 110,556,618 (GRCm39)		probably null	Het
Pxdn	T	C	12: 30,032,726 (GRCm39)	L146S	probably damaging	Het
Rnf32	A	G	5: 29,430,125 (GRCm39)	T315A	probably benign	Het
Rpl5	T	C	5: 108,049,766 (GRCm39)	Y12H	probably benign	Het
Serpinh1	A	T	7: 98,998,521 (GRCm39)	S36R	probably damaging	Het
Setx	A	T	2: 29,051,537 (GRCm39)	T2030S	probably benign	Het
Sf3a3	G	A	4: 124,608,748 (GRCm39)	V21I	probably benign	Het
Sin3b	T	A	8: 73,452,210 (GRCm39)	H105Q	probably damaging	Het
Slitrk1	T	C	14: 109,150,749 (GRCm39)		probably benign	Het
Slx4	A	T	16: 3,805,880 (GRCm39)	D557E	possibly damaging	Het
Sprr3	C	T	3: 92,364,609 (GRCm39)	M78I	probably benign	Het
Ssmem1	A	G	6: 30,519,420 (GRCm39)	E35G	possibly damaging	Het
Stag1	C	T	9: 100,838,461 (GRCm39)	P1238S	probably null	Het
Stra6	C	T	9: 58,059,898 (GRCm39)		probably benign	Het
Tmem127	T	C	2: 127,098,979 (GRCm39)	V171A	probably damaging	Het
Tmem150a	A	G	6: 72,335,742 (GRCm39)		probably null	Het
Top2a	C	G	11: 98,905,886 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,644,771 (GRCm39)		probably null	Het
Tusc3	G	A	8: 39,530,421 (GRCm39)	G129R	possibly damaging	Het
Uspl1	A	G	5: 149,146,515 (GRCm39)	Y422C	probably damaging	Het
Vmn2r88	A	T	14: 51,651,597 (GRCm39)	T312S	probably benign	Het
Zc3hav1l	A	T	6: 38,272,125 (GRCm39)	S215T	probably damaging	Het
Zfp947	T	A	17: 22,365,165 (GRCm39)	T170S	probably benign	Het

Other mutations in Or2h15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02276:Or2h15	APN	17	38,441,484 (GRCm39)	missense	probably damaging	0.97
IGL02659:Or2h15	APN	17	38,441,427 (GRCm39)	missense	possibly damaging	0.78
IGL02831:Or2h15	APN	17	38,441,403 (GRCm39)	missense	probably benign	0.10
R0070:Or2h15	UTSW	17	38,441,780 (GRCm39)	missense	probably damaging	1.00
R1162:Or2h15	UTSW	17	38,441,984 (GRCm39)	missense	possibly damaging	0.69
R2697:Or2h15	UTSW	17	38,441,900 (GRCm39)	missense	probably damaging	0.99
R4694:Or2h15	UTSW	17	38,441,748 (GRCm39)	missense	probably damaging	1.00
R4883:Or2h15	UTSW	17	38,441,508 (GRCm39)	missense	probably damaging	1.00
R4933:Or2h15	UTSW	17	38,441,441 (GRCm39)	missense	probably damaging	1.00
R4982:Or2h15	UTSW	17	38,441,468 (GRCm39)	missense	probably damaging	0.99
R5058:Or2h15	UTSW	17	38,441,432 (GRCm39)	missense	probably damaging	1.00
R5653:Or2h15	UTSW	17	38,442,075 (GRCm39)	missense	possibly damaging	0.63
R5946:Or2h15	UTSW	17	38,441,598 (GRCm39)	missense	probably benign	0.07
R7083:Or2h15	UTSW	17	38,441,601 (GRCm39)	missense	probably benign
R7226:Or2h15	UTSW	17	38,441,324 (GRCm39)	missense	probably benign	0.01
R7391:Or2h15	UTSW	17	38,441,941 (GRCm39)	missense	probably benign
R8297:Or2h15	UTSW	17	38,441,484 (GRCm39)	missense	probably damaging	0.97
R8378:Or2h15	UTSW	17	38,441,678 (GRCm39)	missense	probably benign	0.05
R8425:Or2h15	UTSW	17	38,441,927 (GRCm39)	missense	possibly damaging	0.83
R8554:Or2h15	UTSW	17	38,441,489 (GRCm39)	missense	probably damaging	1.00
R9223:Or2h15	UTSW	17	38,442,012 (GRCm39)	missense	possibly damaging	0.66
R9278:Or2h15	UTSW	17	38,441,693 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTACTACCCGCTGAGAGCAAAAGG -3'
(R):5'- AATTCTCCCACAGAGCATGGGCAC -3'

Sequencing Primer
(F):5'- CCTGAGGGTAGCAGTAGATTG -3'
(R):5'- GGCACAGTTTGCAATGACAC -3'

Posted On

2014-06-10