Mutagenetix > Incidental Mutation

Incidental Mutation 'R1789:Tg'

201667

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

039820-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R1789 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66670753-66850721 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 66737548 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 319 (Q319L)

Ref Sequence

ENSEMBL: ENSMUSP00000126454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000163495] [ENSMUST00000171045]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000065916
AA Change: Q1938L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: Q1938L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163495

SMART Domains

Protein: ENSMUSP00000129868
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
TY	14	63	1.21e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000169163

Predicted Effect

probably benign
Transcript: ENSMUST00000171045
AA Change: Q319L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469
AA Change: Q319L

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172153

SMART Domains

Protein: ENSMUSP00000128410
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
Pfam:COesterase	313	849	6e-140	PFAM

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	G	A	16: 20,365,951 (GRCm38)	P986L	probably damaging	Het
Acad10	A	T	5: 121,631,393 (GRCm38)	Y667N	possibly damaging	Het
Ahi1	G	A	10: 20,963,115 (GRCm38)	G121D	probably benign	Het
Ampd1	T	A	3: 103,099,126 (GRCm38)	I690N	possibly damaging	Het
Amy1	C	A	3: 113,558,165 (GRCm38)	W425L	possibly damaging	Het
Arhgap27	A	T	11: 103,333,005 (GRCm38)	V823E	probably damaging	Het
Arhgef17	A	G	7: 100,929,870 (GRCm38)	S624P	probably damaging	Het
Arid5b	T	C	10: 68,186,067 (GRCm38)	H231R	probably damaging	Het
Aspscr1	C	A	11: 120,688,560 (GRCm38)	T78N	probably damaging	Het
Auts2	T	A	5: 131,472,450 (GRCm38)	T42S	probably damaging	Het
Ccdc171	A	G	4: 83,554,808 (GRCm38)	D158G	probably damaging	Het
Ces4a	G	A	8: 105,138,097 (GRCm38)	G69S	probably damaging	Het
Cfap61	T	C	2: 145,939,993 (GRCm38)		probably null	Het
Chrna5	T	C	9: 55,004,651 (GRCm38)	V245A	possibly damaging	Het
Cntnap5c	G	A	17: 58,013,921 (GRCm38)	G163R	probably damaging	Het
Col5a2	A	G	1: 45,378,305 (GRCm38)		probably null	Het
Col5a2	T	C	1: 45,394,776 (GRCm38)	Q759R	probably damaging	Het
Comp	A	G	8: 70,377,146 (GRCm38)	D340G	probably benign	Het
Cyfip1	T	A	7: 55,926,395 (GRCm38)	D1104E	probably damaging	Het
Dnah11	A	T	12: 118,038,780 (GRCm38)	S308T	probably damaging	Het
Dnah5	A	G	15: 28,270,426 (GRCm38)	H958R	probably benign	Het
Elp3	A	G	14: 65,547,919 (GRCm38)	Y478H	probably damaging	Het
Fam237b	A	T	5: 5,575,652 (GRCm38)	Q116L	possibly damaging	Het
Fat3	T	C	9: 16,376,985 (GRCm38)	Y414C	probably benign	Het
Fbxo10	C	A	4: 45,046,389 (GRCm38)	A574S	probably damaging	Het
Fsip2	G	T	2: 82,977,562 (GRCm38)	L1408F	probably benign	Het
Fubp3	T	C	2: 31,611,735 (GRCm38)	V425A	possibly damaging	Het
Gm7168	A	T	17: 13,949,584 (GRCm38)	R404S	probably benign	Het
Gpd1	T	G	15: 99,723,202 (GRCm38)	F299C	probably damaging	Het
Gpr137	C	T	19: 6,942,057 (GRCm38)		probably benign	Het
Grin3b	T	C	10: 79,973,408 (GRCm38)	S331P	probably benign	Het
Grk3	T	A	5: 112,941,718 (GRCm38)	I281F	probably damaging	Het
Hoxb7	T	A	11: 96,286,781 (GRCm38)	S18R	probably damaging	Het
Igf1r	C	T	7: 68,214,933 (GRCm38)	R1160*	probably null	Het
Itfg1	A	G	8: 85,725,512 (GRCm38)		probably null	Het
Itgb8	T	C	12: 119,202,455 (GRCm38)	I114V	probably benign	Het
Kcnk1	A	G	8: 126,025,384 (GRCm38)	E243G	possibly damaging	Het
Kif27	A	G	13: 58,344,008 (GRCm38)	L439P	probably damaging	Het
Kif2c	T	A	4: 117,167,361 (GRCm38)	Q279L	probably benign	Het
Kmt2d	A	T	15: 98,852,074 (GRCm38)		probably benign	Het
L3mbtl1	A	G	2: 162,974,502 (GRCm38)	T821A	probably benign	Het
Lrrc24	A	T	15: 76,722,578 (GRCm38)	M206K	probably benign	Het
Mamdc4	T	G	2: 25,567,622 (GRCm38)	K460Q	possibly damaging	Het
Maml2	C	G	9: 13,697,345 (GRCm38)	L30V	probably damaging	Het
Mc5r	C	G	18: 68,338,670 (GRCm38)		probably null	Het
Myo10	T	G	15: 25,726,525 (GRCm38)		probably null	Het
Myo1e	T	A	9: 70,338,784 (GRCm38)	L419Q	probably damaging	Het
Myo6	C	A	9: 80,300,572 (GRCm38)	H1115Q	probably damaging	Het
Myo7a	A	G	7: 98,107,095 (GRCm38)	V10A	probably damaging	Het
Myoz2	C	T	3: 123,026,127 (GRCm38)	R61H	probably damaging	Het
Ncdn	G	A	4: 126,752,003 (GRCm38)	R38C	probably damaging	Het
Nckap1	T	A	2: 80,520,556 (GRCm38)	T736S	probably benign	Het
Ncor2	G	A	5: 125,019,890 (GRCm38)	A2325V	probably damaging	Het
Nid2	T	A	14: 19,752,431 (GRCm38)	V140E	possibly damaging	Het
Nlrp9c	C	A	7: 26,380,490 (GRCm38)	D704Y	probably benign	Het
Notch3	T	A	17: 32,158,725 (GRCm38)	S126C	probably damaging	Het
Or2g7	T	C	17: 38,067,948 (GRCm38)	I259T	probably damaging	Het
Or5p60	A	G	7: 108,124,915 (GRCm38)	F116S	probably benign	Het
Or5w10	A	T	2: 87,544,983 (GRCm38)	L187H	probably damaging	Het
Or6c219	A	T	10: 129,945,582 (GRCm38)	L160*	probably null	Het
Or6c6c	T	C	10: 129,705,607 (GRCm38)	M243T	possibly damaging	Het
Or6d15	A	C	6: 116,582,697 (GRCm38)	F83C	probably damaging	Het
Pdzd7	A	G	19: 45,039,228 (GRCm38)	I269T	probably damaging	Het
Phf3	A	T	1: 30,806,206 (GRCm38)	D1299E	probably damaging	Het
Pitx2	T	A	3: 129,218,754 (GRCm38)	Y271N	probably damaging	Het
Polk	T	C	13: 96,496,632 (GRCm38)	E301G	probably damaging	Het
Prkdc	C	A	16: 15,739,524 (GRCm38)	N2230K	probably damaging	Het
Prlr	A	G	15: 10,322,536 (GRCm38)	E170G	probably benign	Het
Prss55	A	G	14: 64,075,730 (GRCm38)	I235T	probably damaging	Het
Psd3	T	C	8: 67,960,565 (GRCm38)	I724V	probably benign	Het
Rabep2	A	G	7: 126,438,799 (GRCm38)	T248A	possibly damaging	Het
Rbm26	T	A	14: 105,117,073 (GRCm38)	K949N	probably benign	Het
Rnf213	T	A	11: 119,440,221 (GRCm38)	D2085E	probably damaging	Het
Serpinb7	A	T	1: 107,450,273 (GRCm38)	H232L	possibly damaging	Het
Slu7	A	G	11: 43,445,242 (GRCm38)	Q484R	probably benign	Het
Smg1	A	G	7: 118,145,798 (GRCm38)	S3044P	possibly damaging	Het
Snrnp48	A	G	13: 38,221,360 (GRCm38)	D248G	possibly damaging	Het
Snrpc	C	A	17: 27,845,219 (GRCm38)	P66Q	unknown	Het
Snrpn	T	A	7: 59,983,459 (GRCm38)		probably benign	Het
Spag17	T	A	3: 99,939,356 (GRCm38)	S65R	possibly damaging	Het
Srsf6	C	A	2: 162,934,488 (GRCm38)		probably benign	Het
Stil	T	A	4: 115,041,782 (GRCm38)	M1203K	probably benign	Het
Syt17	T	C	7: 118,436,838 (GRCm38)	T106A	probably benign	Het
Tbx15	C	T	3: 99,352,246 (GRCm38)	Q478*	probably null	Het
Thada	A	G	17: 84,448,033 (GRCm38)	L243P	probably damaging	Het
Thada	G	T	17: 84,448,034 (GRCm38)	L243I	probably damaging	Het
Tnnt3	A	G	7: 142,512,364 (GRCm38)	R211G	probably damaging	Het
Togaram1	A	T	12: 65,002,635 (GRCm38)	Q1282L	possibly damaging	Het
Tpm3-rs7	T	G	14: 113,314,947 (GRCm38)	M91R	probably damaging	Het
Trappc9	G	A	15: 73,025,967 (GRCm38)	R377W	probably damaging	Het
Ttll7	T	G	3: 146,915,780 (GRCm38)	L378R	probably damaging	Het
Tyw1	T	G	5: 130,258,993 (GRCm38)	I22R	probably damaging	Het
Ubr3	T	C	2: 70,016,367 (GRCm38)	S1645P	possibly damaging	Het
Ubr4	T	C	4: 139,393,053 (GRCm38)	L263P	probably damaging	Het
Unc13c	T	C	9: 73,756,339 (GRCm38)	E1071G	possibly damaging	Het
Vmn2r44	G	T	7: 8,380,123 (GRCm38)	D157E	possibly damaging	Het
Vps16	C	T	2: 130,443,600 (GRCm38)	T821I	probably benign	Het
Washc4	T	C	10: 83,579,525 (GRCm38)	V793A	possibly damaging	Het
Wdr35	G	T	12: 8,977,435 (GRCm38)		probably null	Het
Zfp277	A	G	12: 40,364,085 (GRCm38)	F254L	probably benign	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,847,166 (GRCm38)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,827,290 (GRCm38)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,693,424 (GRCm38)	missense	probably benign
IGL00428:Tg	APN	15	66,773,424 (GRCm38)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,696,489 (GRCm38)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,683,813 (GRCm38)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,688,801 (GRCm38)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,674,073 (GRCm38)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,764,453 (GRCm38)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,681,882 (GRCm38)	missense	probably benign
IGL01288:Tg	APN	15	66,736,276 (GRCm38)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,696,092 (GRCm38)	splice site	probably benign
IGL01634:Tg	APN	15	66,729,566 (GRCm38)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,678,087 (GRCm38)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,671,351 (GRCm38)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,759,486 (GRCm38)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,692,374 (GRCm38)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,705,330 (GRCm38)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,717,233 (GRCm38)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,705,348 (GRCm38)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,757,943 (GRCm38)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,764,291 (GRCm38)	missense	probably benign
IGL02434:Tg	APN	15	66,764,342 (GRCm38)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,741,594 (GRCm38)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,705,274 (GRCm38)	missense	probably benign
IGL02549:Tg	APN	15	66,839,361 (GRCm38)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,748,726 (GRCm38)	splice site	probably benign
IGL02756:Tg	APN	15	66,734,586 (GRCm38)	missense	probably benign
IGL02800:Tg	APN	15	66,757,886 (GRCm38)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,682,394 (GRCm38)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,671,405 (GRCm38)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,715,106 (GRCm38)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,839,303 (GRCm38)	nonsense	probably null
IGL03242:Tg	APN	15	66,683,798 (GRCm38)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
foster	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
hognose	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
ito	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
ito2	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
ito3	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
ito4	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
Papua	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
pluribus	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
samarai	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
sariba	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
ticker	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
Vampire	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,740,718 (GRCm38)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,740,781 (GRCm38)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,698,446 (GRCm38)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,764,442 (GRCm38)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,828,533 (GRCm38)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,682,404 (GRCm38)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,729,597 (GRCm38)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,729,626 (GRCm38)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,737,521 (GRCm38)	missense	probably benign
R0673:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,839,404 (GRCm38)	splice site	probably benign
R0704:Tg	UTSW	15	66,757,880 (GRCm38)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,678,789 (GRCm38)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,725,144 (GRCm38)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,708,010 (GRCm38)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,672,409 (GRCm38)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,698,559 (GRCm38)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,684,062 (GRCm38)	missense	probably benign
R1103:Tg	UTSW	15	66,719,655 (GRCm38)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,828,548 (GRCm38)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,696,489 (GRCm38)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,850,502 (GRCm38)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,705,232 (GRCm38)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,693,430 (GRCm38)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,729,685 (GRCm38)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,696,166 (GRCm38)	nonsense	probably null
R1655:Tg	UTSW	15	66,828,568 (GRCm38)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,692,387 (GRCm38)	missense	possibly damaging	0.89
R1883:Tg	UTSW	15	66,671,309 (GRCm38)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,682,842 (GRCm38)	missense	probably benign
R2063:Tg	UTSW	15	66,828,553 (GRCm38)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,849,607 (GRCm38)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,729,594 (GRCm38)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,681,939 (GRCm38)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,681,933 (GRCm38)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,683,898 (GRCm38)	missense	probably benign
R2994:Tg	UTSW	15	66,681,953 (GRCm38)	missense	probably benign
R3904:Tg	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
R3946:Tg	UTSW	15	66,674,023 (GRCm38)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,684,190 (GRCm38)	missense	probably benign
R4245:Tg	UTSW	15	66,696,469 (GRCm38)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,766,147 (GRCm38)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,707,942 (GRCm38)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,735,271 (GRCm38)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,673,920 (GRCm38)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,693,319 (GRCm38)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,764,337 (GRCm38)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,696,586 (GRCm38)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,707,930 (GRCm38)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,681,813 (GRCm38)	splice site	probably null
R5049:Tg	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
R5073:Tg	UTSW	15	66,735,252 (GRCm38)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,678,780 (GRCm38)	nonsense	probably null
R5185:Tg	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,759,567 (GRCm38)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,678,855 (GRCm38)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,678,055 (GRCm38)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,739,168 (GRCm38)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,827,293 (GRCm38)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,685,300 (GRCm38)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,693,435 (GRCm38)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,838,057 (GRCm38)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,688,889 (GRCm38)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,683,993 (GRCm38)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,828,457 (GRCm38)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,673,367 (GRCm38)	splice site	probably null
R6159:Tg	UTSW	15	66,735,247 (GRCm38)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,707,922 (GRCm38)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,671,311 (GRCm38)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,759,558 (GRCm38)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,735,259 (GRCm38)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,688,891 (GRCm38)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,696,246 (GRCm38)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,693,358 (GRCm38)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,673,543 (GRCm38)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,694,784 (GRCm38)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,725,272 (GRCm38)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,696,583 (GRCm38)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,696,588 (GRCm38)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,696,161 (GRCm38)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,694,768 (GRCm38)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,689,927 (GRCm38)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,764,418 (GRCm38)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,729,583 (GRCm38)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,849,604 (GRCm38)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,693,263 (GRCm38)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,683,814 (GRCm38)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,705,279 (GRCm38)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,684,074 (GRCm38)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,683,793 (GRCm38)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,773,398 (GRCm38)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
R8688:Tg	UTSW	15	66,694,953 (GRCm38)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,681,937 (GRCm38)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,684,042 (GRCm38)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,685,335 (GRCm38)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,773,483 (GRCm38)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,683,673 (GRCm38)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,698,461 (GRCm38)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,827,269 (GRCm38)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,685,397 (GRCm38)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,689,324 (GRCm38)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,847,074 (GRCm38)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,674,064 (GRCm38)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,735,260 (GRCm38)	nonsense	probably null
R9629:Tg	UTSW	15	66,683,738 (GRCm38)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,766,142 (GRCm38)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,689,990 (GRCm38)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,847,159 (GRCm38)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,682,454 (GRCm38)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,748,743 (GRCm38)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,849,547 (GRCm38)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,685,310 (GRCm38)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- ACCCCAGGCTTGTCATGAAC -3'
(R):5'- TGGCGTCATATCTCTGAAGGAC -3'

Sequencing Primer
(F):5'- GGCTTGTCATGAACTAAACACC -3'
(R):5'- CGTCATATCTCTGAAGGACCATGG -3'

Posted On

2014-06-23