Mutagenetix > Incidental Mutation

Incidental Mutation 'R1793:Clcn1'

201925

Institutional Source

Beutler Lab

Gene Symbol

Clcn1

Ensembl Gene

ENSMUSG00000029862

Gene Name

chloride channel, voltage-sensitive 1

Synonyms

Clc1, SMCC1, NMF355, Clc-1, nmf355

MMRRC Submission

039823-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1793 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42263619-42292690 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to A at 42275860 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000031894 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031894] [ENSMUST00000164091] [ENSMUST00000168660]

AlphaFold

Q64347

Predicted Effect

probably null
Transcript: ENSMUST00000031894

SMART Domains

Protein: ENSMUSP00000031894
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	121	130	N/A	INTRINSIC
Pfam:Voltage_CLC	170	572	3.2e-87	PFAM
Blast:CBS	612	662	1e-24	BLAST
low complexity region	723	747	N/A	INTRINSIC
Blast:CBS	830	877	4e-19	BLAST
low complexity region	928	950	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000114684

SMART Domains

Protein: ENSMUSP00000110332
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
Pfam:Voltage_CLC	3	254	2.6e-42	PFAM
CBS	294	344	1.3e1	SMART
low complexity region	405	429	N/A	INTRINSIC
Blast:CBS	480	524	2e-13	BLAST
low complexity region	575	597	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000163936

SMART Domains

Protein: ENSMUSP00000130148
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	92	101	N/A	INTRINSIC
Pfam:Voltage_CLC	141	261	1.2e-27	PFAM
Pfam:Voltage_CLC	258	501	3.9e-44	PFAM
PDB:2D4Z\|B	520	807	2e-47	PDB
Blast:CBS	541	591	2e-24	BLAST
Blast:CBS	759	806	3e-19	BLAST
low complexity region	857	879	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000164091

SMART Domains

Protein: ENSMUSP00000131354
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	121	130	N/A	INTRINSIC
Pfam:Voltage_CLC	170	256	2.9e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000165780

SMART Domains

Protein: ENSMUSP00000130550
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	92	101	N/A	INTRINSIC
Pfam:Voltage_CLC	141	227	9.7e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000168660

SMART Domains

Protein: ENSMUSP00000126045
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	88	97	N/A	INTRINSIC
Pfam:Voltage_CLC	136	257	1.1e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000169024

SMART Domains

Protein: ENSMUSP00000130968
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	92	101	N/A	INTRINSIC
Pfam:Voltage_CLC	141	261	2.9e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000170028

SMART Domains

Protein: ENSMUSP00000132154
Gene: ENSMUSG00000029862

Domain	Start	End	E-Value	Type
low complexity region	92	101	N/A	INTRINSIC
Pfam:Voltage_CLC	141	235	8e-22	PFAM

Meta Mutation Damage Score

0.9495

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

100% (122/122)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
PHENOTYPE: Mutant mice exhibit mild to severe spasms of the hind limbs and abnormal hind limb reflexes. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 118 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,884,144 (GRCm39)	H571Q	probably damaging	Het
Abcf2	T	A	5: 24,773,774 (GRCm39)	M372L	probably benign	Het
Acaca	A	C	11: 84,206,795 (GRCm39)	T1552P	probably damaging	Het
Acaca	A	G	11: 84,229,219 (GRCm39)	D1682G	probably damaging	Het
Acvr1b	T	C	15: 101,091,906 (GRCm39)	V62A	probably benign	Het
Aimp1	C	T	3: 132,379,825 (GRCm39)	V97M	probably benign	Het
Aldh1l1	C	T	6: 90,554,813 (GRCm39)	T557I	possibly damaging	Het
Alpk1	T	C	3: 127,471,447 (GRCm39)	T1012A	probably damaging	Het
Amot	G	A	X: 144,233,585 (GRCm39)		probably benign	Het
Arap1	T	A	7: 101,037,829 (GRCm39)	H477Q	probably benign	Het
Arhgap18	T	A	10: 26,736,732 (GRCm39)		probably benign	Het
Arhgef10l	C	T	4: 140,242,684 (GRCm39)	V862M	probably damaging	Het
Arl4d	A	G	11: 101,557,554 (GRCm39)	I27V	probably benign	Het
Asb18	G	A	1: 89,942,277 (GRCm39)	P8L	probably damaging	Het
Ash1l	A	G	3: 88,977,616 (GRCm39)	H2682R	probably damaging	Het
Aspm	T	A	1: 139,385,079 (GRCm39)	V241E	probably benign	Het
Atf7ip	G	T	6: 136,586,217 (GRCm39)		probably benign	Het
AY358078	T	G	14: 52,042,051 (GRCm39)	M142R	unknown	Het
C3	T	A	17: 57,526,592 (GRCm39)	K796N	possibly damaging	Het
Ceacam16	T	C	7: 19,590,041 (GRCm39)	T301A	probably damaging	Het
Ceacam5	T	G	7: 17,481,320 (GRCm39)	Y356D	probably benign	Het
Cep192	C	T	18: 67,984,838 (GRCm39)	A1616V	possibly damaging	Het
Cfap91	T	A	16: 38,141,781 (GRCm39)	N384Y	possibly damaging	Het
Cherp	A	T	8: 73,216,994 (GRCm39)	H645Q	probably benign	Het
CK137956	T	C	4: 127,845,242 (GRCm39)	D167G	probably benign	Het
Cntn1	C	T	15: 92,189,552 (GRCm39)	T625I	possibly damaging	Het
Cpn2	T	A	16: 30,078,142 (GRCm39)	N520Y	probably damaging	Het
Crocc	G	A	4: 140,746,620 (GRCm39)	R1762W	probably damaging	Het
Cts3	C	A	13: 61,715,967 (GRCm39)	V100F	probably benign	Het
Ddx56	A	T	11: 6,216,934 (GRCm39)	V87D	probably damaging	Het
Dnah9	C	T	11: 66,010,420 (GRCm39)		probably null	Het
Dock1	T	A	7: 134,700,456 (GRCm39)		probably null	Het
Dst	T	A	1: 34,191,552 (GRCm39)	Y291*	probably null	Het
Eif4g3	T	C	4: 137,898,442 (GRCm39)	I1071T	probably damaging	Het
Fblim1	T	C	4: 141,322,549 (GRCm39)	Q78R	probably damaging	Het
Fcamr	C	T	1: 130,739,284 (GRCm39)	P195S	probably benign	Het
Fcho1	G	A	8: 72,161,666 (GRCm39)	Q835*	probably null	Het
Frem3	A	G	8: 81,339,741 (GRCm39)	N678S	probably benign	Het
Frk	G	A	10: 34,483,878 (GRCm39)	R413H	probably benign	Het
Gm10619	C	A	7: 73,459,758 (GRCm39)		noncoding transcript	Het
Gm12185	G	A	11: 48,806,583 (GRCm39)	R203*	probably null	Het
Gm12886	A	G	4: 121,280,174 (GRCm39)	V34A	probably benign	Het
Gna12	A	T	5: 140,746,707 (GRCm39)	I246N	probably damaging	Het
Gpm6a	T	C	8: 55,507,867 (GRCm39)	M201T	probably benign	Het
Gpsm2	T	G	3: 108,608,225 (GRCm39)	D220A	probably benign	Het
Grip2	C	G	6: 91,760,623 (GRCm39)	V325L	probably benign	Het
Grk5	C	A	19: 61,065,200 (GRCm39)	A288D	probably damaging	Het
Herpud2	G	A	9: 25,021,953 (GRCm39)	A231V	possibly damaging	Het
Hmcn1	T	C	1: 150,624,834 (GRCm39)	S1024G	probably benign	Het
Hp1bp3	A	G	4: 137,957,820 (GRCm39)	D295G	probably damaging	Het
Igsf21	T	C	4: 139,761,703 (GRCm39)	H325R	probably damaging	Het
Ing2	T	A	8: 48,122,364 (GRCm39)	L61F	probably damaging	Het
Jak3	T	C	8: 72,138,590 (GRCm39)		probably benign	Het
Kcnn3	T	A	3: 89,516,712 (GRCm39)	C374S	probably benign	Het
Klk1b22	A	G	7: 43,765,775 (GRCm39)		probably benign	Het
Larp1	T	C	11: 57,940,764 (GRCm39)	M630T	possibly damaging	Het
Lhx5	T	A	5: 120,572,725 (GRCm39)	C115S	probably damaging	Het
Lmcd1	C	A	6: 112,305,712 (GRCm39)	T271K	probably benign	Het
Lpar1	G	A	4: 58,486,798 (GRCm39)	R158*	probably null	Het
Lyst	T	A	13: 13,821,668 (GRCm39)	C1347*	probably null	Het
Map3k8	G	A	18: 4,332,389 (GRCm39)	Q441*	probably null	Het
Mboat1	T	C	13: 30,403,633 (GRCm39)	V144A	probably damaging	Het
Mcur1	C	T	13: 43,713,491 (GRCm39)	G38S	unknown	Het
Med13	T	A	11: 86,220,177 (GRCm39)	M276L	probably benign	Het
Mefv	A	G	16: 3,526,528 (GRCm39)	S699P	possibly damaging	Het
Mfsd4a	C	A	1: 131,987,077 (GRCm39)	A62S	probably damaging	Het
Mmp27	A	G	9: 7,571,459 (GRCm39)	M1V	probably null	Het
Myo1c	C	T	11: 75,548,415 (GRCm39)	T58I	probably damaging	Het
Myom3	A	T	4: 135,538,066 (GRCm39)	D1316V	probably benign	Het
Naa25	T	A	5: 121,555,478 (GRCm39)	C235S	possibly damaging	Het
Naa25	C	A	5: 121,558,656 (GRCm39)	R333S	probably damaging	Het
Nav3	G	A	10: 109,539,233 (GRCm39)	T2056I	probably benign	Het
Nol4	A	G	18: 22,902,878 (GRCm39)	Y378H	probably damaging	Het
Npat	G	A	9: 53,463,589 (GRCm39)	R124Q	probably damaging	Het
Npr3	T	A	15: 11,848,665 (GRCm39)	E434V	probably benign	Het
Nptx2	A	G	5: 144,485,130 (GRCm39)	T208A	probably benign	Het
Obscn	C	T	11: 58,968,606 (GRCm39)	V2798M	probably damaging	Het
Odad4	C	A	11: 100,460,679 (GRCm39)		probably null	Het
Or10j7	A	T	1: 173,011,976 (GRCm39)	H8Q	probably benign	Het
Or52r1	A	G	7: 102,537,250 (GRCm39)	Y37H	probably benign	Het
Or5b122	C	T	19: 13,562,779 (GRCm39)	T37I	probably benign	Het
Padi1	G	A	4: 140,541,967 (GRCm39)	P652S	probably damaging	Het
Pcdh1	G	T	18: 38,331,938 (GRCm39)	P355Q	probably damaging	Het
Pck2	G	A	14: 55,781,422 (GRCm39)	R189H	possibly damaging	Het
Pcsk5	T	A	19: 17,432,114 (GRCm39)	K1500N	possibly damaging	Het
Phc3	A	T	3: 31,002,865 (GRCm39)	S218T	probably damaging	Het
Piezo2	T	A	18: 63,239,355 (GRCm39)	M510L	possibly damaging	Het
Polr1g	T	C	7: 19,091,904 (GRCm39)	T68A	possibly damaging	Het
Ppp1r3a	A	T	6: 14,754,717 (GRCm39)	Y177N	probably damaging	Het
Psme2b	A	T	11: 48,836,361 (GRCm39)	D195E	probably damaging	Het
Ptprr	T	C	10: 116,088,827 (GRCm39)	V463A	probably damaging	Het
Pwwp2b	G	A	7: 138,836,281 (GRCm39)	R574Q	probably damaging	Het
Rap1gds1	T	A	3: 138,756,314 (GRCm39)	T14S	possibly damaging	Het
Rbm11	A	T	16: 75,397,685 (GRCm39)	K205M	probably damaging	Het
Rfx1	T	A	8: 84,793,050 (GRCm39)		probably benign	Het
Rnasel	C	A	1: 153,630,169 (GRCm39)	H228Q	probably damaging	Het
Sap130	T	A	18: 31,831,640 (GRCm39)	I710K	probably benign	Het
Slc27a4	C	A	2: 29,695,733 (GRCm39)	D89E	probably benign	Het
Spata31d1b	T	C	13: 59,863,779 (GRCm39)	V309A	probably benign	Het
Syt7	T	G	19: 10,421,354 (GRCm39)	Y420D	probably damaging	Het
Tanc2	A	G	11: 105,515,859 (GRCm39)		probably null	Het
Tbc1d10b	A	G	7: 126,802,930 (GRCm39)	S333P	possibly damaging	Het
Tenm2	G	A	11: 35,914,209 (GRCm39)	P2442S	probably damaging	Het
Tenm3	C	T	8: 49,127,579 (GRCm39)	C33Y	probably damaging	Het
Timmdc1	A	G	16: 38,319,419 (GRCm39)	L245P	possibly damaging	Het
Tlr5	T	C	1: 182,800,012 (GRCm39)	F5L	probably benign	Het
Ttll4	T	A	1: 74,726,999 (GRCm39)	F784L	possibly damaging	Het
Tulp4	A	G	17: 6,189,387 (GRCm39)	T70A	possibly damaging	Het
Txndc17	T	A	11: 72,099,571 (GRCm39)	N81K	probably benign	Het
Ubr3	C	T	2: 69,830,895 (GRCm39)		probably benign	Het
Uri1	A	C	7: 37,681,116 (GRCm39)	V96G	probably damaging	Het
Uspl1	T	A	5: 149,150,246 (GRCm39)	I482N	probably damaging	Het
Vmn1r229	A	G	17: 21,034,974 (GRCm39)	N73S	possibly damaging	Het
Vwa7	G	T	17: 35,243,388 (GRCm39)	G689*	probably null	Het
Zfp114	T	A	7: 23,877,164 (GRCm39)		probably null	Het
Zfp618	A	G	4: 63,051,474 (GRCm39)	S659G	probably damaging	Het
Zfp872	A	G	9: 22,111,349 (GRCm39)	K275R	probably damaging	Het
Zzef1	C	T	11: 72,777,535 (GRCm39)	P1789S	probably damaging	Het

Other mutations in Clcn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01473:Clcn1	APN	6	42,268,637 (GRCm39)	missense	probably damaging	1.00
IGL01732:Clcn1	APN	6	42,287,606 (GRCm39)	splice site	probably benign
IGL02055:Clcn1	APN	6	42,284,489 (GRCm39)	missense	probably damaging	1.00
IGL02507:Clcn1	APN	6	42,284,007 (GRCm39)	splice site	probably benign
IGL02649:Clcn1	APN	6	42,275,763 (GRCm39)	missense	probably damaging	1.00
IGL02739:Clcn1	APN	6	42,263,714 (GRCm39)	splice site	probably null
IGL03148:Clcn1	APN	6	42,276,925 (GRCm39)	critical splice donor site	probably null
IGL03190:Clcn1	APN	6	42,267,037 (GRCm39)	missense	probably benign	0.02
IGL03327:Clcn1	APN	6	42,288,153 (GRCm39)	missense	probably benign	0.00
IGL03346:Clcn1	APN	6	42,288,153 (GRCm39)	missense	probably benign	0.00
Faint	UTSW	6	42,284,199 (GRCm39)	missense	probably damaging	1.00
jack_spratt	UTSW	6	42,287,515 (GRCm39)	missense	probably benign
Limitations	UTSW	6	42,286,997 (GRCm39)	missense	possibly damaging	0.79
maimed	UTSW	6	42,275,754 (GRCm39)	missense	probably damaging	1.00
stunted	UTSW	6	42,263,701 (GRCm39)	start codon destroyed	possibly damaging	0.79
R0167:Clcn1	UTSW	6	42,263,770 (GRCm39)	missense	probably damaging	1.00
R0323:Clcn1	UTSW	6	42,287,074 (GRCm39)	missense	probably damaging	0.99
R0491:Clcn1	UTSW	6	42,287,515 (GRCm39)	missense	probably benign
R0573:Clcn1	UTSW	6	42,289,979 (GRCm39)	splice site	probably null
R0615:Clcn1	UTSW	6	42,282,509 (GRCm39)	missense	probably damaging	1.00
R0944:Clcn1	UTSW	6	42,290,075 (GRCm39)	missense	probably benign	0.00
R1562:Clcn1	UTSW	6	42,277,169 (GRCm39)	missense	probably benign	0.29
R1566:Clcn1	UTSW	6	42,268,374 (GRCm39)	missense	possibly damaging	0.58
R1692:Clcn1	UTSW	6	42,290,032 (GRCm39)	missense	possibly damaging	0.67
R1728:Clcn1	UTSW	6	42,276,448 (GRCm39)	missense	possibly damaging	0.86
R1729:Clcn1	UTSW	6	42,276,448 (GRCm39)	missense	possibly damaging	0.86
R1772:Clcn1	UTSW	6	42,271,079 (GRCm39)	missense	probably damaging	1.00
R1784:Clcn1	UTSW	6	42,276,448 (GRCm39)	missense	possibly damaging	0.86
R1861:Clcn1	UTSW	6	42,290,925 (GRCm39)	missense	possibly damaging	0.63
R1864:Clcn1	UTSW	6	42,282,475 (GRCm39)	missense	probably damaging	1.00
R1865:Clcn1	UTSW	6	42,282,475 (GRCm39)	missense	probably damaging	1.00
R2356:Clcn1	UTSW	6	42,268,559 (GRCm39)	missense	probably damaging	1.00
R2403:Clcn1	UTSW	6	42,290,046 (GRCm39)	missense	probably damaging	0.99
R2987:Clcn1	UTSW	6	42,275,784 (GRCm39)	missense	probably damaging	1.00
R3082:Clcn1	UTSW	6	42,267,112 (GRCm39)	missense	probably damaging	0.98
R3500:Clcn1	UTSW	6	42,269,929 (GRCm39)	missense	probably damaging	0.99
R3747:Clcn1	UTSW	6	42,276,849 (GRCm39)	missense	probably damaging	1.00
R3748:Clcn1	UTSW	6	42,276,849 (GRCm39)	missense	probably damaging	1.00
R4041:Clcn1	UTSW	6	42,286,902 (GRCm39)	missense	probably damaging	1.00
R4749:Clcn1	UTSW	6	42,267,131 (GRCm39)	splice site	probably null
R4836:Clcn1	UTSW	6	42,286,898 (GRCm39)	missense	probably damaging	0.96
R5021:Clcn1	UTSW	6	42,287,922 (GRCm39)	nonsense	probably null
R5085:Clcn1	UTSW	6	42,290,814 (GRCm39)	missense	probably benign	0.41
R5528:Clcn1	UTSW	6	42,277,275 (GRCm39)	missense	probably benign	0.01
R5628:Clcn1	UTSW	6	42,275,823 (GRCm39)	missense	probably damaging	0.96
R5678:Clcn1	UTSW	6	42,284,199 (GRCm39)	missense	probably damaging	1.00
R5943:Clcn1	UTSW	6	42,269,900 (GRCm39)	missense	probably damaging	1.00
R6053:Clcn1	UTSW	6	42,277,208 (GRCm39)	nonsense	probably null
R6175:Clcn1	UTSW	6	42,291,096 (GRCm39)	missense	probably damaging	1.00
R6394:Clcn1	UTSW	6	42,290,172 (GRCm39)	missense	possibly damaging	0.82
R6394:Clcn1	UTSW	6	42,284,524 (GRCm39)	missense	possibly damaging	0.84
R7012:Clcn1	UTSW	6	42,267,542 (GRCm39)	missense	probably benign	0.01
R7020:Clcn1	UTSW	6	42,275,754 (GRCm39)	missense	probably damaging	1.00
R7048:Clcn1	UTSW	6	42,284,477 (GRCm39)	missense	probably damaging	1.00
R7212:Clcn1	UTSW	6	42,268,323 (GRCm39)	missense	possibly damaging	0.46
R7225:Clcn1	UTSW	6	42,270,396 (GRCm39)	missense	probably damaging	1.00
R7264:Clcn1	UTSW	6	42,275,772 (GRCm39)	missense	probably damaging	1.00
R7636:Clcn1	UTSW	6	42,268,268 (GRCm39)	nonsense	probably null
R7663:Clcn1	UTSW	6	42,286,997 (GRCm39)	missense	possibly damaging	0.79
R7807:Clcn1	UTSW	6	42,287,282 (GRCm39)	splice site	probably null
R7954:Clcn1	UTSW	6	42,263,625 (GRCm39)	unclassified	probably benign
R8026:Clcn1	UTSW	6	42,284,595 (GRCm39)	critical splice donor site	probably null
R8045:Clcn1	UTSW	6	42,267,628 (GRCm39)	missense	probably damaging	1.00
R8499:Clcn1	UTSW	6	42,284,133 (GRCm39)	missense	probably damaging	1.00
R8523:Clcn1	UTSW	6	42,284,523 (GRCm39)	nonsense	probably null
R8677:Clcn1	UTSW	6	42,267,519 (GRCm39)	critical splice acceptor site	probably null
R8818:Clcn1	UTSW	6	42,282,477 (GRCm39)	missense	probably damaging	0.98
R8945:Clcn1	UTSW	6	42,263,701 (GRCm39)	start codon destroyed	possibly damaging	0.79
R9012:Clcn1	UTSW	6	42,268,567 (GRCm39)	missense	possibly damaging	0.75
R9295:Clcn1	UTSW	6	42,290,883 (GRCm39)	missense	probably benign	0.00
R9433:Clcn1	UTSW	6	42,282,494 (GRCm39)	missense	probably damaging	1.00
R9513:Clcn1	UTSW	6	42,282,462 (GRCm39)	missense	probably damaging	1.00
R9679:Clcn1	UTSW	6	42,263,753 (GRCm39)	missense	probably damaging	0.98
Z1088:Clcn1	UTSW	6	42,284,190 (GRCm39)	missense	probably damaging	1.00
Z1088:Clcn1	UTSW	6	42,277,294 (GRCm39)	missense	probably benign	0.40
Z1176:Clcn1	UTSW	6	42,284,501 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAGACTCACAACTCAGGTG -3'
(R):5'- CCTACTAGCATCTCGCCATG -3'

Sequencing Primer
(F):5'- AGGTGAAGCTCCTGAGGTC -3'
(R):5'- GCCATGCTTATACTCTCAAACCATG -3'

Posted On

2014-06-23