Mutagenetix > Incidental Mutation

Incidental Mutation 'R1795:Cyp4f17'

202190

Institutional Source

Beutler Lab

Gene Symbol

Cyp4f17

Ensembl Gene

ENSMUSG00000091586

Gene Name

cytochrome P450, family 4, subfamily f, polypeptide 17

Synonyms

EG208285

MMRRC Submission

039825-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R1795 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32725404-32749132 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 32736943 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 92 (I92F)

Ref Sequence

ENSEMBL: ENSMUSP00000129514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165999]

AlphaFold

G3UW78

Predicted Effect

probably benign
Transcript: ENSMUST00000165999
AA Change: I92F

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000129514
Gene: ENSMUSG00000091586
AA Change: I92F

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
Pfam:p450	52	515	3.5e-135	PFAM

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	A	G	11: 109,941,792 (GRCm39)	I1159T	probably benign	Het
Abcc1	T	C	16: 14,283,001 (GRCm39)	V1159A	possibly damaging	Het
Abcg5	A	G	17: 84,981,007 (GRCm39)	I194T	probably damaging	Het
Abtb2	A	G	2: 103,397,369 (GRCm39)	T100A	probably benign	Het
Adam4	C	T	12: 81,468,068 (GRCm39)	M184I	probably benign	Het
Afap1l2	T	C	19: 56,916,841 (GRCm39)	D155G	probably damaging	Het
Ahnak	T	C	19: 8,979,802 (GRCm39)	V362A	possibly damaging	Het
Ak7	C	T	12: 105,692,482 (GRCm39)	R179*	probably null	Het
Akap8	C	A	17: 32,534,451 (GRCm39)	G332C	probably damaging	Het
Akr1e1	T	A	13: 4,645,071 (GRCm39)	Q204L	probably damaging	Het
Ankrd12	T	C	17: 66,293,222 (GRCm39)	E737G	possibly damaging	Het
Atrn	A	G	2: 130,814,208 (GRCm39)	D718G	probably benign	Het
Bco2	A	G	9: 50,452,469 (GRCm39)	S200P	possibly damaging	Het
C030005K15Rik	A	T	10: 97,561,648 (GRCm39)	S28T	unknown	Het
Cdc14a	T	A	3: 116,092,122 (GRCm39)	Q356L	possibly damaging	Het
Cdk5rap3	A	G	11: 96,799,654 (GRCm39)	L387P	probably damaging	Het
Celsr1	A	T	15: 85,914,524 (GRCm39)	S1150T	probably damaging	Het
Cntfr	A	G	4: 41,670,841 (GRCm39)		probably null	Het
Cramp1	T	C	17: 25,183,884 (GRCm39)	N1244D	probably damaging	Het
Csmd3	G	T	15: 47,721,316 (GRCm39)	D1542E	possibly damaging	Het
Dhx30	A	T	9: 109,937,051 (GRCm39)		probably null	Het
Dlg1	T	A	16: 31,561,965 (GRCm39)	H120Q	probably benign	Het
Dmgdh	A	T	13: 93,843,207 (GRCm39)	M348L	probably benign	Het
Dnmt3b	G	A	2: 153,525,559 (GRCm39)	E741K	possibly damaging	Het
Dock3	T	G	9: 106,902,534 (GRCm39)	H292P	probably damaging	Het
Ercc6	T	A	14: 32,238,985 (GRCm39)	N24K	probably benign	Het
Esco2	T	C	14: 66,064,726 (GRCm39)	Q338R	probably benign	Het
Etl4	T	C	2: 20,812,837 (GRCm39)		probably null	Het
Exoc7	A	T	11: 116,183,347 (GRCm39)	I498N	probably damaging	Het
Fap	G	A	2: 62,378,933 (GRCm39)	S123L	probably damaging	Het
Foxn1	T	C	11: 78,262,051 (GRCm39)	E106G	probably benign	Het
Fscb	T	A	12: 64,521,175 (GRCm39)	D97V	probably damaging	Het
Gabrg1	T	C	5: 70,939,596 (GRCm39)	T174A	possibly damaging	Het
Gan	C	T	8: 117,923,199 (GRCm39)	A461V	possibly damaging	Het
Gbp2	T	A	3: 142,336,284 (GRCm39)	D211E	possibly damaging	Het
Gm10271	A	T	10: 116,792,746 (GRCm39)	Y47N	unknown	Het
Gm17333	G	T	16: 77,649,711 (GRCm39)		noncoding transcript	Het
Gm1758	T	A	16: 14,320,142 (GRCm39)		noncoding transcript	Het
Golga3	A	G	5: 110,355,493 (GRCm39)	K989R	possibly damaging	Het
Gucy2g	C	A	19: 55,187,973 (GRCm39)	V1041F	probably damaging	Het
Guk1	A	T	11: 59,077,639 (GRCm39)	F25I	probably benign	Het
H2al1o	C	T	X: 9,438,329 (GRCm39)	E87K	possibly damaging	Het
Hemgn	C	A	4: 46,395,958 (GRCm39)	C426F	probably damaging	Het
Hps3	A	G	3: 20,066,859 (GRCm39)		probably null	Het
Il2rb	A	T	15: 78,368,187 (GRCm39)	D287E	probably damaging	Het
Ino80	A	T	2: 119,237,340 (GRCm39)	V1123D	probably damaging	Het
Kdm2b	A	G	5: 123,122,523 (GRCm39)		probably null	Het
Kif21a	A	T	15: 90,856,930 (GRCm39)		probably null	Het
Klhl33	A	T	14: 51,129,583 (GRCm39)	N347K	probably damaging	Het
Krt1c	A	G	15: 101,724,861 (GRCm39)	F250L	possibly damaging	Het
Krt82	T	A	15: 101,451,819 (GRCm39)	N332I	possibly damaging	Het
Lgi1	A	G	19: 38,294,631 (GRCm39)	I444V	probably benign	Het
Lmod1	G	A	1: 135,252,862 (GRCm39)	V39M	probably damaging	Het
Lonrf2	G	A	1: 38,852,357 (GRCm39)	P165S	probably benign	Het
Mex3d	T	C	10: 80,217,376 (GRCm39)	T149A	probably benign	Het
Mideas	A	G	12: 84,205,748 (GRCm39)		probably null	Het
Mlxipl	A	C	5: 135,136,024 (GRCm39)	D83A	probably damaging	Het
Mroh8	T	A	2: 157,111,471 (GRCm39)	E161V	probably benign	Het
Mroh9	G	A	1: 162,884,347 (GRCm39)	T397I	probably damaging	Het
Mtss1	T	C	15: 58,930,249 (GRCm39)	D32G	possibly damaging	Het
Mus81	T	C	19: 5,533,504 (GRCm39)	D495G	probably benign	Het
Neurod1	A	C	2: 79,284,673 (GRCm39)	S237A	probably benign	Het
Npas1	G	A	7: 16,208,725 (GRCm39)	R51C	probably damaging	Het
Or11g27	T	C	14: 50,771,159 (GRCm39)	S97P	possibly damaging	Het
P2ry14	T	C	3: 59,023,274 (GRCm39)	N62S	probably damaging	Het
Pcdh15	A	G	10: 74,460,087 (GRCm39)	Y1308C	probably damaging	Het
Pcdhb14	A	T	18: 37,582,588 (GRCm39)	M565L	probably benign	Het
Pde6a	A	G	18: 61,390,283 (GRCm39)	E502G	probably damaging	Het
Pde8b	A	G	13: 95,178,527 (GRCm39)	V566A	probably benign	Het
Phf11b	G	T	14: 59,565,554 (GRCm39)	Q108K	probably benign	Het
Pikfyve	T	C	1: 65,291,716 (GRCm39)	Y1312H	probably damaging	Het
Plcb3	T	C	19: 6,933,381 (GRCm39)		probably benign	Het
Plxna2	G	A	1: 194,488,611 (GRCm39)	G1629D	probably damaging	Het
Plxnb1	T	C	9: 108,929,813 (GRCm39)	V223A	probably benign	Het
Prkca	A	T	11: 107,903,518 (GRCm39)	Y285N	possibly damaging	Het
Prl2a1	A	T	13: 27,992,554 (GRCm39)	N226I	probably damaging	Het
Pus7	A	C	5: 23,946,914 (GRCm39)	M636R	probably damaging	Het
Samd9l	G	A	6: 3,375,264 (GRCm39)	Q666*	probably null	Het
Sema3d	A	G	5: 12,634,854 (GRCm39)	D640G	probably benign	Het
Slc6a15	A	G	10: 103,236,121 (GRCm39)	I279V	probably benign	Het
Slk	T	C	19: 47,608,973 (GRCm39)	V642A	possibly damaging	Het
Spata31e2	C	A	1: 26,722,070 (GRCm39)	G1037*	probably null	Het
Spta1	T	A	1: 174,073,296 (GRCm39)	M2305K	probably damaging	Het
Srrt	T	C	5: 137,301,274 (GRCm39)		probably benign	Het
Stfa2l1	A	T	16: 35,977,228 (GRCm39)	I8L	probably benign	Het
Tap1	T	C	17: 34,413,899 (GRCm39)	L638P	probably benign	Het
Tbccd1	A	T	16: 22,640,995 (GRCm39)	L461M	probably benign	Het
Tecta	T	C	9: 42,289,345 (GRCm39)	T407A	probably benign	Het
Tmbim7	T	C	5: 3,707,493 (GRCm39)		probably null	Het
Tnrc18	C	A	5: 142,800,869 (GRCm39)	V30L	probably benign	Het
Tomm7	A	G	5: 24,049,025 (GRCm39)	F16S	probably damaging	Het
Ugt2a2	A	G	5: 87,622,315 (GRCm39)	S428P	probably benign	Het
Vmn1r189	T	C	13: 22,286,324 (GRCm39)	E171G	probably benign	Het
Vmn1r61	T	C	7: 5,614,324 (GRCm39)		probably benign	Het
Vmn2r120	A	T	17: 57,832,038 (GRCm39)	S250R	probably benign	Het
Vmn2r8	T	C	5: 108,950,972 (GRCm39)	R158G	probably benign	Het
Vmn2r98	A	T	17: 19,286,702 (GRCm39)	Y400F	probably damaging	Het
Vps13c	C	A	9: 67,801,267 (GRCm39)	Y582*	probably null	Het
Zfp518a	T	A	19: 40,904,000 (GRCm39)	F1310I	probably benign	Het
Zswim1	A	G	2: 164,667,320 (GRCm39)	I191V	probably benign	Het

Other mutations in Cyp4f17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00580:Cyp4f17	APN	17	32,743,849 (GRCm39)	nonsense	probably null
IGL01767:Cyp4f17	APN	17	32,725,956 (GRCm39)	missense	probably benign
IGL01867:Cyp4f17	APN	17	32,747,057 (GRCm39)	missense	probably benign	0.30
IGL02009:Cyp4f17	APN	17	32,743,854 (GRCm39)	missense	probably damaging	1.00
IGL02423:Cyp4f17	APN	17	32,725,923 (GRCm39)	missense	possibly damaging	0.93
IGL02503:Cyp4f17	APN	17	32,743,940 (GRCm39)	critical splice donor site	probably null
IGL02571:Cyp4f17	APN	17	32,743,878 (GRCm39)	missense	probably benign	0.42
IGL03328:Cyp4f17	APN	17	32,739,600 (GRCm39)	missense	probably damaging	1.00
IGL03047:Cyp4f17	UTSW	17	32,743,023 (GRCm39)	missense	possibly damaging	0.88
PIT4810001:Cyp4f17	UTSW	17	32,743,574 (GRCm39)	missense	possibly damaging	0.56
R0486:Cyp4f17	UTSW	17	32,743,797 (GRCm39)	splice site	probably benign
R0606:Cyp4f17	UTSW	17	32,746,817 (GRCm39)	missense	probably damaging	0.98
R0655:Cyp4f17	UTSW	17	32,743,871 (GRCm39)	missense	possibly damaging	0.95
R1781:Cyp4f17	UTSW	17	32,742,993 (GRCm39)	missense	possibly damaging	0.94
R1833:Cyp4f17	UTSW	17	32,743,184 (GRCm39)	missense	probably benign	0.01
R2268:Cyp4f17	UTSW	17	32,736,928 (GRCm39)	missense	probably benign	0.44
R3030:Cyp4f17	UTSW	17	32,725,950 (GRCm39)	missense	possibly damaging	0.93
R3861:Cyp4f17	UTSW	17	32,747,078 (GRCm39)	missense	probably damaging	0.97
R5236:Cyp4f17	UTSW	17	32,739,606 (GRCm39)	critical splice donor site	probably null
R5450:Cyp4f17	UTSW	17	32,747,860 (GRCm39)	missense	probably benign
R5866:Cyp4f17	UTSW	17	32,725,887 (GRCm39)	missense	probably benign	0.03
R5886:Cyp4f17	UTSW	17	32,743,013 (GRCm39)	missense	possibly damaging	0.78
R5965:Cyp4f17	UTSW	17	32,743,611 (GRCm39)	missense	probably damaging	0.99
R6692:Cyp4f17	UTSW	17	32,725,950 (GRCm39)	missense	possibly damaging	0.93
R7056:Cyp4f17	UTSW	17	32,746,846 (GRCm39)	missense	possibly damaging	0.94
R7968:Cyp4f17	UTSW	17	32,743,116 (GRCm39)	missense	possibly damaging	0.87
R8458:Cyp4f17	UTSW	17	32,739,550 (GRCm39)	missense	probably damaging	1.00
R8550:Cyp4f17	UTSW	17	32,746,936 (GRCm39)	missense	probably benign	0.01
R8818:Cyp4f17	UTSW	17	32,743,068 (GRCm39)	missense	probably damaging	1.00
R9139:Cyp4f17	UTSW	17	32,743,868 (GRCm39)	nonsense	probably null
R9360:Cyp4f17	UTSW	17	32,743,880 (GRCm39)	missense	probably benign	0.25
R9375:Cyp4f17	UTSW	17	32,747,746 (GRCm39)	missense	probably damaging	0.97
R9690:Cyp4f17	UTSW	17	32,725,950 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CACGTTGATTCAGCAAGAGC -3'
(R):5'- GTCAGTCAGGTTAGTGGCAG -3'

Sequencing Primer
(F):5'- CGTTGATTCAGCAAGAGCCATAAGTG -3'
(R):5'- CAGTGGATTCTATTAGATTGGCTCC -3'

Posted On

2014-06-23