Mutagenetix > Incidental Mutation

Incidental Mutation 'R0092:Frem2'

20263

Institutional Source

Beutler Lab

Gene Symbol

Frem2

Ensembl Gene

ENSMUSG00000037016

Gene Name

Fras1 related extracellular matrix protein 2

Synonyms

my, ne, 6030440P17Rik, b2b1562Clo, 8430406N05Rik

MMRRC Submission

038379-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0092 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

53513938-53657355 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 53589796 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 1766 (Y1766H)

Ref Sequence

ENSEMBL: ENSMUSP00000088670 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091137]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000091137
AA Change: Y1766H

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000088670
Gene: ENSMUSG00000037016
AA Change: Y1766H

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
Pfam:Cadherin_3	249	388	4.3e-9	PFAM
Pfam:Cadherin_3	376	532	3e-34	PFAM
Pfam:Cadherin_3	516	665	7.5e-24	PFAM
Pfam:Cadherin_3	632	798	1.6e-21	PFAM
Pfam:Cadherin_3	763	910	1.2e-25	PFAM
Pfam:Cadherin_3	879	1027	5.1e-18	PFAM
Pfam:Cadherin_3	1015	1159	2.2e-20	PFAM
CA	1202	1293	4.8e-1	SMART
Pfam:Cadherin_3	1392	1503	9.8e-24	PFAM
Pfam:Cadherin_3	1504	1612	6.2e-28	PFAM
Pfam:Cadherin_3	1613	1743	5.3e-20	PFAM
Calx_beta	1748	1847	1.5e-5	SMART
Calx_beta	1860	1971	9.47e-12	SMART
Calx_beta	1985	2092	1.65e-11	SMART
Calx_beta	2105	2209	1.99e-5	SMART
Calx_beta	2227	2331	6.9e-14	SMART
transmembrane domain	3103	3125	N/A	INTRINSIC

Meta Mutation Damage Score

0.0633

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.1%
20x: 88.6%

Validation Efficiency

99% (112/113)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Kidney development is severely affected and syndactyly is common. Phenotypes of homozygous mutants are indistinguishable from those of Fras1 homozygous mutant. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438A13Rik	A	G	3: 37,028,159 (GRCm38)	D3790G	probably benign	Het
Abcg2	T	A	6: 58,685,777 (GRCm38)	S535T	probably benign	Het
Acad11	A	T	9: 104,090,341 (GRCm38)		probably benign	Het
Acadm	A	T	3: 153,941,875 (GRCm38)		probably benign	Het
Acot12	T	A	13: 91,741,565 (GRCm38)	M12K	probably damaging	Het
Actr2	A	T	11: 20,094,308 (GRCm38)	N99K	probably benign	Het
Adam2	G	A	14: 66,053,887 (GRCm38)	A314V	probably damaging	Het
Aes	G	A	10: 81,561,220 (GRCm38)	G10D	possibly damaging	Het
Agl	C	T	3: 116,793,804 (GRCm38)	R34Q	probably damaging	Het
Agrn	C	T	4: 156,178,953 (GRCm38)	R338H	probably damaging	Het
AI661453	A	G	17: 47,467,515 (GRCm38)		probably benign	Het
Alpk3	A	G	7: 81,092,553 (GRCm38)	D706G	probably benign	Het
Apbb1	T	C	7: 105,559,154 (GRCm38)	E648G	probably damaging	Het
Astn2	C	A	4: 66,403,982 (GRCm38)	A127S	unknown	Het
Asxl2	T	C	12: 3,496,313 (GRCm38)	S366P	probably benign	Het
Bdh1	A	T	16: 31,447,562 (GRCm38)	K92*	probably null	Het
Cacna1g	C	T	11: 94,457,264 (GRCm38)	S666N	probably damaging	Het
Ces2b	A	G	8: 104,836,512 (GRCm38)	T361A	possibly damaging	Het
Col6a4	T	A	9: 106,013,314 (GRCm38)	E1927V	probably benign	Het
Ctnnb1	T	G	9: 120,952,863 (GRCm38)	I314S	possibly damaging	Het
Cyp2c66	T	C	19: 39,183,780 (GRCm38)		probably benign	Het
Dennd4c	T	A	4: 86,781,607 (GRCm38)	F232I	probably damaging	Het
Dennd5a	T	C	7: 109,899,806 (GRCm38)	N950S	possibly damaging	Het
Dhx30	T	C	9: 110,085,010 (GRCm38)	N14S	possibly damaging	Het
Dip2b	T	A	15: 100,202,265 (GRCm38)	V1004D	probably damaging	Het
Dnah1	A	C	14: 31,271,609 (GRCm38)	S2872A	probably benign	Het
Dnajc10	T	C	2: 80,325,682 (GRCm38)	V233A	probably damaging	Het
E230025N22Rik	A	G	18: 36,689,224 (GRCm38)	L162P	probably damaging	Het
Elmod3	T	C	6: 72,566,809 (GRCm38)	D333G	probably benign	Het
Epb41l3	T	A	17: 69,286,750 (GRCm38)	M846K	probably damaging	Het
Fxr2	T	C	11: 69,642,146 (GRCm38)		probably benign	Het
Gm14085	G	T	2: 122,517,597 (GRCm38)		probably benign	Het
Gm9833	G	A	3: 10,088,573 (GRCm38)	C134Y	possibly damaging	Het
Gmpr2	A	G	14: 55,677,945 (GRCm38)	R258G	probably benign	Het
Helb	T	C	10: 120,089,808 (GRCm38)	Y888C	probably damaging	Het
Hephl1	TTCCAGATGTCC	TTCC	9: 15,090,603 (GRCm38)		probably null	Het
Hipk2	T	C	6: 38,743,229 (GRCm38)	D482G	probably damaging	Het
Itgb4	G	T	11: 115,979,124 (GRCm38)	R44L	probably damaging	Het
Itih1	T	C	14: 30,940,863 (GRCm38)		probably benign	Het
Kit	T	A	5: 75,647,754 (GRCm38)	S719R	possibly damaging	Het
Krt13	G	A	11: 100,121,432 (GRCm38)	Q22*	probably null	Het
L3mbtl4	A	C	17: 68,425,703 (GRCm38)	R59S	probably benign	Het
Lpp	A	G	16: 24,761,602 (GRCm38)	S23G	probably benign	Het
Magi3	G	A	3: 104,050,964 (GRCm38)	Q602*	probably null	Het
Man2a1	A	G	17: 64,659,084 (GRCm38)		probably benign	Het
Muc5ac	A	G	7: 141,818,630 (GRCm38)	E2667G	possibly damaging	Het
Myo15b	C	G	11: 115,862,986 (GRCm38)	S842C	possibly damaging	Het
Naf1	T	A	8: 66,889,108 (GRCm38)	S462T	probably benign	Het
Necab3	T	C	2: 154,558,739 (GRCm38)	D34G	possibly damaging	Het
Nisch	C	A	14: 31,191,453 (GRCm38)		probably benign	Het
Nlrc5	T	C	8: 94,489,594 (GRCm38)		probably benign	Het
Nmt1	T	C	11: 103,046,493 (GRCm38)	F119L	probably damaging	Het
Nod1	T	G	6: 54,944,541 (GRCm38)	D264A	probably damaging	Het
Nol8	C	T	13: 49,662,447 (GRCm38)	A677V	possibly damaging	Het
Nt5e	T	A	9: 88,370,285 (GRCm38)	F567I	probably benign	Het
Obscn	A	T	11: 59,051,247 (GRCm38)	M4434K	possibly damaging	Het
Olfr119	T	G	17: 37,700,805 (GRCm38)	L45R	probably damaging	Het
Olfr50	A	G	2: 36,793,496 (GRCm38)	T87A	probably benign	Het
Olfr512	T	C	7: 108,713,824 (GRCm38)	V145A	probably benign	Het
Olfr632	T	C	7: 103,937,727 (GRCm38)	S116P	probably damaging	Het
Olfr796	A	G	10: 129,608,221 (GRCm38)	S87P	probably damaging	Het
Opa1	A	T	16: 29,625,594 (GRCm38)	D866V	probably damaging	Het
Otop1	T	A	5: 38,299,830 (GRCm38)	V311E	probably damaging	Het
Pcsk2	A	G	2: 143,801,024 (GRCm38)	D407G	probably damaging	Het
Pdcd1	A	G	1: 94,052,424 (GRCm38)	W23R	possibly damaging	Het
Pigp	A	G	16: 94,365,462 (GRCm38)	V129A	probably damaging	Het
Pik3r5	A	G	11: 68,492,803 (GRCm38)	R483G	probably benign	Het
Pink1	A	G	4: 138,319,998 (GRCm38)	V225A	probably benign	Het
Plcl1	C	G	1: 55,696,765 (GRCm38)	Q422E	probably damaging	Het
Plec	T	C	15: 76,183,743 (GRCm38)	E1222G	probably benign	Het
Polr1a	T	C	6: 71,967,455 (GRCm38)		probably benign	Het
Prokr2	C	T	2: 132,373,597 (GRCm38)	V154M	probably damaging	Het
Rasgrp4	A	G	7: 29,145,132 (GRCm38)	R280G	possibly damaging	Het
Rmnd5b	T	C	11: 51,629,592 (GRCm38)	E8G	possibly damaging	Het
Sbf2	T	A	7: 110,320,806 (GRCm38)		probably benign	Het
Sec23b	A	G	2: 144,566,910 (GRCm38)	M172V	probably benign	Het
Setx	T	C	2: 29,146,293 (GRCm38)	V930A	probably benign	Het
Sft2d2	G	A	1: 165,179,260 (GRCm38)	A159V	possibly damaging	Het
Sh3gl1	G	T	17: 56,018,088 (GRCm38)	R250S	probably benign	Het
Skor1	C	A	9: 63,145,995 (GRCm38)	D231Y	probably damaging	Het
Slc24a1	T	G	9: 64,948,752 (GRCm38)	E291A	unknown	Het
Smc1b	A	T	15: 85,067,724 (GRCm38)		probably benign	Het
Tbccd1	A	T	16: 22,826,094 (GRCm38)	N177K	possibly damaging	Het
Tdp1	T	A	12: 99,954,989 (GRCm38)	Y595N	probably damaging	Het
Tmem108	T	C	9: 103,489,305 (GRCm38)	K496E	possibly damaging	Het
Tmprss7	T	C	16: 45,667,596 (GRCm38)	D490G	probably damaging	Het
Tnrc6b	A	T	15: 80,918,528 (GRCm38)	N1511Y	probably damaging	Het
Top2b	G	A	14: 16,409,263 (GRCm38)	R802Q	probably damaging	Het
Trip10	A	T	17: 57,250,798 (GRCm38)	K27N	possibly damaging	Het
Txlnb	A	G	10: 17,842,755 (GRCm38)	N445D	possibly damaging	Het
Txnrd1	T	A	10: 82,879,802 (GRCm38)	I159N	probably damaging	Het
Ulk1	C	A	5: 110,796,327 (GRCm38)	A164S	probably null	Het
Vmn2r83	T	C	10: 79,491,964 (GRCm38)	V802A	probably damaging	Het
Zbtb4	A	G	11: 69,779,351 (GRCm38)	I967V	probably benign	Het

Other mutations in Frem2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Frem2	APN	3	53,585,595 (GRCm38)	missense	probably damaging	1.00
IGL00911:Frem2	APN	3	53,572,462 (GRCm38)	missense	probably damaging	1.00
IGL01322:Frem2	APN	3	53,541,038 (GRCm38)	missense	probably benign	0.00
IGL01330:Frem2	APN	3	53,655,241 (GRCm38)	missense	possibly damaging	0.70
IGL01406:Frem2	APN	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
IGL01556:Frem2	APN	3	53,535,281 (GRCm38)	missense	probably benign	0.23
IGL01580:Frem2	APN	3	53,655,175 (GRCm38)	missense	probably damaging	1.00
IGL01606:Frem2	APN	3	53,653,591 (GRCm38)	missense	possibly damaging	0.69
IGL01611:Frem2	APN	3	53,655,709 (GRCm38)	missense	probably benign	0.00
IGL01648:Frem2	APN	3	53,535,732 (GRCm38)	missense	possibly damaging	0.86
IGL01663:Frem2	APN	3	53,517,013 (GRCm38)	missense	probably damaging	1.00
IGL01665:Frem2	APN	3	53,549,662 (GRCm38)	missense	probably benign	0.07
IGL01670:Frem2	APN	3	53,656,937 (GRCm38)	missense	possibly damaging	0.95
IGL01960:Frem2	APN	3	53,522,304 (GRCm38)	missense	probably benign	0.33
IGL02175:Frem2	APN	3	53,655,599 (GRCm38)	missense	possibly damaging	0.69
IGL02201:Frem2	APN	3	53,519,640 (GRCm38)	missense	probably benign	0.35
IGL02202:Frem2	APN	3	53,654,799 (GRCm38)	missense	probably benign	0.00
IGL02427:Frem2	APN	3	53,535,763 (GRCm38)	missense	probably damaging	0.97
IGL02457:Frem2	APN	3	53,521,049 (GRCm38)	missense	probably damaging	0.99
IGL02638:Frem2	APN	3	53,551,346 (GRCm38)	missense	possibly damaging	0.94
IGL02801:Frem2	APN	3	53,652,175 (GRCm38)	missense	possibly damaging	0.85
IGL03023:Frem2	APN	3	53,655,628 (GRCm38)	missense	probably benign	0.40
IGL03169:Frem2	APN	3	53,522,292 (GRCm38)	missense	probably benign	0.01
IGL03238:Frem2	APN	3	53,656,261 (GRCm38)	missense	possibly damaging	0.93
IGL03251:Frem2	APN	3	53,572,308 (GRCm38)	missense	probably benign	0.01
IGL03273:Frem2	APN	3	53,537,509 (GRCm38)	nonsense	probably null
IGL03343:Frem2	APN	3	53,652,253 (GRCm38)	missense	probably damaging	1.00
Biosimilar	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
Fruit_stripe	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
PIT4366001:Frem2	UTSW	3	53,653,201 (GRCm38)	missense	probably damaging	0.98
R0019:Frem2	UTSW	3	53,523,678 (GRCm38)	missense	probably damaging	0.99
R0108:Frem2	UTSW	3	53,647,961 (GRCm38)	missense	probably benign	0.03
R0115:Frem2	UTSW	3	53,656,208 (GRCm38)	missense	probably damaging	0.99
R0118:Frem2	UTSW	3	53,535,243 (GRCm38)	nonsense	probably null
R0374:Frem2	UTSW	3	53,653,960 (GRCm38)	missense	probably damaging	1.00
R0437:Frem2	UTSW	3	53,653,015 (GRCm38)	missense	possibly damaging	0.96
R0531:Frem2	UTSW	3	53,519,954 (GRCm38)	missense	probably damaging	1.00
R0555:Frem2	UTSW	3	53,516,860 (GRCm38)	missense	probably damaging	0.97
R0564:Frem2	UTSW	3	53,656,109 (GRCm38)	missense	probably damaging	0.97
R0586:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R0726:Frem2	UTSW	3	53,519,626 (GRCm38)	missense	possibly damaging	0.89
R0925:Frem2	UTSW	3	53,653,973 (GRCm38)	missense	probably benign
R1233:Frem2	UTSW	3	53,547,778 (GRCm38)	missense	probably damaging	0.98
R1302:Frem2	UTSW	3	53,655,538 (GRCm38)	missense	probably benign	0.00
R1333:Frem2	UTSW	3	53,549,731 (GRCm38)	missense	probably benign	0.26
R1446:Frem2	UTSW	3	53,654,596 (GRCm38)	missense	probably benign	0.31
R1523:Frem2	UTSW	3	53,655,407 (GRCm38)	missense	possibly damaging	0.73
R1539:Frem2	UTSW	3	53,654,210 (GRCm38)	missense	probably benign	0.19
R1543:Frem2	UTSW	3	53,572,455 (GRCm38)	missense	possibly damaging	0.86
R1597:Frem2	UTSW	3	53,654,519 (GRCm38)	missense	probably benign	0.19
R1600:Frem2	UTSW	3	53,547,723 (GRCm38)	missense	probably damaging	1.00
R1678:Frem2	UTSW	3	53,519,938 (GRCm38)	missense	probably damaging	1.00
R1687:Frem2	UTSW	3	53,653,952 (GRCm38)	missense	probably benign
R1696:Frem2	UTSW	3	53,656,042 (GRCm38)	nonsense	probably null
R1758:Frem2	UTSW	3	53,653,357 (GRCm38)	missense	probably damaging	1.00
R1857:Frem2	UTSW	3	53,654,873 (GRCm38)	missense	probably benign	0.10
R1869:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.04
R1921:Frem2	UTSW	3	53,653,495 (GRCm38)	missense	possibly damaging	0.76
R1973:Frem2	UTSW	3	53,652,232 (GRCm38)	missense	probably benign	0.01
R2045:Frem2	UTSW	3	53,535,744 (GRCm38)	missense	probably damaging	1.00
R2113:Frem2	UTSW	3	53,652,922 (GRCm38)	missense	probably damaging	1.00
R2152:Frem2	UTSW	3	53,517,029 (GRCm38)	nonsense	probably null
R2164:Frem2	UTSW	3	53,537,330 (GRCm38)	missense	probably damaging	1.00
R2181:Frem2	UTSW	3	53,574,587 (GRCm38)	missense	possibly damaging	0.72
R2201:Frem2	UTSW	3	53,516,573 (GRCm38)	missense	probably benign
R2221:Frem2	UTSW	3	53,516,857 (GRCm38)	missense	probably benign	0.00
R2255:Frem2	UTSW	3	53,652,514 (GRCm38)	missense	probably damaging	0.96
R2280:Frem2	UTSW	3	53,572,423 (GRCm38)	missense	probably damaging	1.00
R3196:Frem2	UTSW	3	53,537,331 (GRCm38)	missense	probably damaging	1.00
R3716:Frem2	UTSW	3	53,572,360 (GRCm38)	missense	probably damaging	1.00
R3807:Frem2	UTSW	3	53,653,449 (GRCm38)	missense	probably benign	0.22
R3820:Frem2	UTSW	3	53,516,849 (GRCm38)	missense	probably damaging	1.00
R3821:Frem2	UTSW	3	53,652,415 (GRCm38)	missense	probably damaging	1.00
R3977:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R3979:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R4014:Frem2	UTSW	3	53,652,353 (GRCm38)	missense	probably benign	0.01
R4127:Frem2	UTSW	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
R4195:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4196:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4374:Frem2	UTSW	3	53,545,502 (GRCm38)	missense	possibly damaging	0.61
R4427:Frem2	UTSW	3	53,539,162 (GRCm38)	critical splice donor site	probably null
R4428:Frem2	UTSW	3	53,654,338 (GRCm38)	missense	probably benign	0.40
R4559:Frem2	UTSW	3	53,654,321 (GRCm38)	missense	probably benign	0.01
R4600:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4602:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4610:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4611:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4661:Frem2	UTSW	3	53,655,443 (GRCm38)	missense	probably damaging	1.00
R4678:Frem2	UTSW	3	53,544,371 (GRCm38)	missense	probably benign	0.00
R4689:Frem2	UTSW	3	53,547,635 (GRCm38)	missense	probably benign	0.43
R4740:Frem2	UTSW	3	53,535,819 (GRCm38)	missense	probably benign	0.04
R4748:Frem2	UTSW	3	53,541,093 (GRCm38)	missense	probably damaging	1.00
R4790:Frem2	UTSW	3	53,516,741 (GRCm38)	missense	probably benign
R4809:Frem2	UTSW	3	53,653,895 (GRCm38)	missense	probably benign	0.01
R4930:Frem2	UTSW	3	53,656,315 (GRCm38)	missense	possibly damaging	0.93
R4971:Frem2	UTSW	3	53,539,183 (GRCm38)	missense	probably damaging	1.00
R5057:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.37
R5202:Frem2	UTSW	3	53,551,346 (GRCm38)	missense	probably benign	0.41
R5221:Frem2	UTSW	3	53,585,611 (GRCm38)	missense	probably damaging	1.00
R5231:Frem2	UTSW	3	53,522,295 (GRCm38)	missense	probably damaging	1.00
R5268:Frem2	UTSW	3	53,653,154 (GRCm38)	missense	probably damaging	0.96
R5480:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R5637:Frem2	UTSW	3	53,652,937 (GRCm38)	missense	probably damaging	0.97
R5664:Frem2	UTSW	3	53,652,490 (GRCm38)	missense	probably benign	0.33
R5698:Frem2	UTSW	3	53,652,505 (GRCm38)	missense	possibly damaging	0.89
R5744:Frem2	UTSW	3	53,655,959 (GRCm38)	missense	probably damaging	1.00
R5754:Frem2	UTSW	3	53,537,258 (GRCm38)	missense	probably damaging	1.00
R5808:Frem2	UTSW	3	53,652,563 (GRCm38)	missense	probably damaging	0.96
R5840:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R5874:Frem2	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
R6050:Frem2	UTSW	3	53,653,012 (GRCm38)	missense	probably damaging	0.99
R6103:Frem2	UTSW	3	53,549,788 (GRCm38)	missense	probably benign	0.00
R6149:Frem2	UTSW	3	53,551,341 (GRCm38)	missense	probably damaging	0.98
R6182:Frem2	UTSW	3	53,647,969 (GRCm38)	missense	probably damaging	1.00
R6191:Frem2	UTSW	3	53,655,280 (GRCm38)	missense	probably benign	0.10
R6245:Frem2	UTSW	3	53,655,824 (GRCm38)	missense	probably benign	0.00
R6252:Frem2	UTSW	3	53,572,448 (GRCm38)	missense	probably damaging	1.00
R6393:Frem2	UTSW	3	53,585,640 (GRCm38)	missense	possibly damaging	0.91
R6416:Frem2	UTSW	3	53,572,378 (GRCm38)	missense	probably benign	0.01
R6595:Frem2	UTSW	3	53,549,784 (GRCm38)	missense	probably damaging	1.00
R6665:Frem2	UTSW	3	53,654,656 (GRCm38)	missense	probably damaging	1.00
R6708:Frem2	UTSW	3	53,585,501 (GRCm38)	missense	probably benign	0.00
R6751:Frem2	UTSW	3	53,653,665 (GRCm38)	missense	probably damaging	1.00
R6787:Frem2	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
R6913:Frem2	UTSW	3	53,516,821 (GRCm38)	missense	probably damaging	1.00
R6916:Frem2	UTSW	3	53,547,688 (GRCm38)	missense	probably damaging	1.00
R7017:Frem2	UTSW	3	53,519,602 (GRCm38)	missense	probably benign	0.02
R7083:Frem2	UTSW	3	53,537,493 (GRCm38)	missense	probably damaging	0.99
R7108:Frem2	UTSW	3	53,653,513 (GRCm38)	missense	probably damaging	1.00
R7133:Frem2	UTSW	3	53,572,339 (GRCm38)	missense	possibly damaging	0.82
R7326:Frem2	UTSW	3	53,654,753 (GRCm38)	missense	probably damaging	1.00
R7341:Frem2	UTSW	3	53,654,495 (GRCm38)	missense	probably damaging	1.00
R7455:Frem2	UTSW	3	53,572,280 (GRCm38)	splice site	probably null
R7487:Frem2	UTSW	3	53,654,549 (GRCm38)	missense	probably benign	0.40
R7495:Frem2	UTSW	3	53,516,837 (GRCm38)	missense	probably benign	0.13
R7542:Frem2	UTSW	3	53,652,579 (GRCm38)	missense	probably damaging	1.00
R7636:Frem2	UTSW	3	53,653,247 (GRCm38)	missense	probably benign	0.00
R7703:Frem2	UTSW	3	53,522,168 (GRCm38)	missense	probably benign	0.01
R7750:Frem2	UTSW	3	53,523,682 (GRCm38)	missense	possibly damaging	0.83
R7849:Frem2	UTSW	3	53,572,374 (GRCm38)	missense	probably damaging	1.00
R7922:Frem2	UTSW	3	53,653,304 (GRCm38)	missense	probably damaging	0.98
R8008:Frem2	UTSW	3	53,652,910 (GRCm38)	missense	probably damaging	1.00
R8051:Frem2	UTSW	3	53,535,355 (GRCm38)	missense	probably benign	0.04
R8052:Frem2	UTSW	3	53,549,643 (GRCm38)	missense	probably benign	0.02
R8176:Frem2	UTSW	3	53,655,340 (GRCm38)	missense	possibly damaging	0.50
R8220:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R8397:Frem2	UTSW	3	53,653,141 (GRCm38)	missense	probably benign	0.00
R8410:Frem2	UTSW	3	53,539,177 (GRCm38)	missense	possibly damaging	0.60
R8697:Frem2	UTSW	3	53,525,828 (GRCm38)	missense	probably damaging	0.99
R9134:Frem2	UTSW	3	53,654,900 (GRCm38)	missense	probably damaging	1.00
R9183:Frem2	UTSW	3	53,520,065 (GRCm38)	missense	probably damaging	1.00
R9260:Frem2	UTSW	3	53,652,783 (GRCm38)	missense	probably damaging	1.00
R9267:Frem2	UTSW	3	53,657,083 (GRCm38)	start codon destroyed	probably null	0.00
R9299:Frem2	UTSW	3	53,656,559 (GRCm38)	missense	probably benign	0.37
R9378:Frem2	UTSW	3	53,651,989 (GRCm38)	missense	probably damaging	0.99
R9444:Frem2	UTSW	3	53,652,844 (GRCm38)	missense	probably benign	0.10
R9459:Frem2	UTSW	3	53,653,486 (GRCm38)	missense	probably benign
R9487:Frem2	UTSW	3	53,653,484 (GRCm38)	missense	possibly damaging	0.95
R9728:Frem2	UTSW	3	53,656,631 (GRCm38)	missense	probably benign	0.00
R9759:Frem2	UTSW	3	53,655,497 (GRCm38)	missense	possibly damaging	0.76
Z1177:Frem2	UTSW	3	53,655,607 (GRCm38)	missense	probably benign	0.31
Z1177:Frem2	UTSW	3	53,535,166 (GRCm38)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCACACTCATGCACCTTGACAC -3'
(R):5'- AGGTTAGCTCCATCTCCATGCACG -3'

Sequencing Primer
(F):5'- GATCTCTTCGTAGCCAAAAATCAG -3'
(R):5'- GCCCTGGAGTTGCATATTAAATCC -3'

Posted On

2013-04-11