Mutagenetix > Incidental Mutation

Incidental Mutation 'R1800:Fras1'

203027

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

039830-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1800 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

96373955-96784728 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 96709882 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 2012 (S2012P)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000036019
AA Change: S2012P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: S2012P

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.0%
20x: 91.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,066,718	K556R	probably damaging	Het
4933406P04Rik	A	T	10: 20,311,147		probably benign	Het
A530099J19Rik	T	C	13: 19,729,679		noncoding transcript	Het
Acmsd	C	A	1: 127,759,756	Y258*	probably null	Het
Adgrf5	A	T	17: 43,451,082	I1223F	probably damaging	Het
Afp	C	T	5: 90,490,796	P6S	probably benign	Het
Agap2	C	A	10: 127,091,671	H1120Q	probably damaging	Het
Angpt1	A	G	15: 42,512,404	V152A	probably damaging	Het
Ankrd1	A	T	19: 36,119,359	F23I	probably damaging	Het
Armc12	T	C	17: 28,538,895	S334P	possibly damaging	Het
Arnt2	C	A	7: 84,275,375	D415Y	probably damaging	Het
Cacna1s	G	T	1: 136,076,854	V131L	probably benign	Het
Cacna2d2	T	C	9: 107,527,433	V1141A	possibly damaging	Het
Cfap61	T	C	2: 146,042,622	L580P	probably damaging	Het
Chga	A	T	12: 102,555,905	S23C	probably damaging	Het
Chrna5	A	G	9: 55,004,875	T320A	probably damaging	Het
Col17a1	G	T	19: 47,650,862	N1081K	possibly damaging	Het
Cox7b2	A	T	5: 71,443,063	C51S	possibly damaging	Het
Cwc22	A	T	2: 77,929,453	D83E	possibly damaging	Het
D130043K22Rik	A	C	13: 24,883,894	D871A	probably damaging	Het
Dab2	A	T	15: 6,435,467	M361L	probably benign	Het
Dnah11	A	C	12: 117,916,788	Y3866D	probably damaging	Het
Dnmbp	A	G	19: 43,901,720	I536T	probably benign	Het
Ehmt1	A	T	2: 24,884,290	S43R	probably damaging	Het
Ercc5	T	C	1: 44,173,380	L666P	probably benign	Het
F5	A	G	1: 164,182,834	H378R	probably damaging	Het
Fam84a	T	C	12: 14,150,225	Y167C	probably damaging	Het
Fat2	A	T	11: 55,283,892	N1998K	probably damaging	Het
Fbxo28	T	C	1: 182,341,534	D13G	unknown	Het
Gm4758	T	A	16: 36,312,533	D57E	probably damaging	Het
Gm5624	T	A	14: 44,561,845	N41I	probably damaging	Het
Gm6768	A	T	12: 119,261,771		noncoding transcript	Het
Gm8104	A	G	14: 43,101,571	I73V	possibly damaging	Het
Gpr68	G	A	12: 100,878,908	P126S	probably damaging	Het
Gucy1a2	A	T	9: 3,582,685	T156S	possibly damaging	Het
Hacl1	T	A	14: 31,614,264	Y429F	probably damaging	Het
Haus3	A	C	5: 34,163,572	L510R	probably damaging	Het
Helq	C	T	5: 100,774,124	G738D	probably benign	Het
Hsd3b2	G	C	3: 98,712,237	P131A	probably damaging	Het
Il22ra1	G	T	4: 135,748,160	C247F	probably benign	Het
Ivl	T	C	3: 92,572,584	E58G	unknown	Het
Kcnk9	G	T	15: 72,546,099	Q61K	probably benign	Het
Kremen1	CGGG	CGGGGGG	11: 5,201,791		probably benign	Het
Loxhd1	G	T	18: 77,402,502	A1314S	probably damaging	Het
Lrba	A	G	3: 86,351,868	I1397V	probably benign	Het
Lrig3	T	A	10: 125,997,051		probably null	Het
Lrrc36	A	G	8: 105,449,765	D378G	probably damaging	Het
Lrrc49	G	A	9: 60,598,191	T527I	probably damaging	Het
Map4k4	C	A	1: 40,023,460	T1195K	probably damaging	Het
Mrps33	G	A	6: 39,802,495	R89C	probably damaging	Het
Mtor	T	C	4: 148,462,892	V444A	probably benign	Het
Myh2	A	G	11: 67,188,938	E1046G	probably damaging	Het
Myo15b	G	T	11: 115,880,509		probably null	Het
Myo5c	T	C	9: 75,246,164	Y111H	probably damaging	Het
Ndufv2	G	C	17: 66,083,486	T163R	probably damaging	Het
Nf1	A	G	11: 79,553,968	N373S	possibly damaging	Het
Nwd2	A	G	5: 63,805,574	M834V	probably benign	Het
Ogfod2	T	C	5: 124,114,956	V343A	probably damaging	Het
Olfr1220	T	A	2: 89,097,706	T74S	probably benign	Het
Olfr401	A	T	11: 74,121,408	T40S	probably benign	Het
Olfr597	T	A	7: 103,320,414	M1K	probably null	Het
Olfr610	T	C	7: 103,506,041	I302V	possibly damaging	Het
Olfr736	C	A	14: 50,393,329	S191*	probably null	Het
Olfr744	C	T	14: 50,618,686	L155F	probably benign	Het
Olfr954	A	T	9: 39,462,114	I228F	probably damaging	Het
Parp1	T	A	1: 180,600,526		probably null	Het
Ppp3ca	A	T	3: 136,935,031	I448F	probably damaging	Het
Prss44	T	C	9: 110,817,204	V333A	probably damaging	Het
Pxk	C	T	14: 8,151,507	R441*	probably null	Het
Rars	A	C	11: 35,825,995	D231E	probably benign	Het
Rfc1	T	C	5: 65,264,379	Y1059C	probably damaging	Het
Rilpl1	T	A	5: 124,514,656	I2F	probably damaging	Het
Sarm1	G	T	11: 78,490,994	A221E	possibly damaging	Het
Serpina3f	A	G	12: 104,217,406	T176A	probably damaging	Het
Slc22a20	T	C	19: 5,985,667	E131G	probably benign	Het
Slc26a11	T	C	11: 119,373,153	V343A	probably damaging	Het
Slc2a4	A	G	11: 69,946,307	I9T	probably benign	Het
Slc39a6	G	T	18: 24,585,202	D599E	probably damaging	Het
Slc5a6	G	T	5: 31,040,676	S285*	probably null	Het
Spata9	A	G	13: 75,977,641	H81R	probably benign	Het
St7l	A	G	3: 104,919,496	E436G	probably damaging	Het
Tecrl	A	G	5: 83,279,230	I357T	probably damaging	Het
Thop1	C	T	10: 81,073,209	R25C	probably damaging	Het
Tmprss12	A	G	15: 100,292,547	E266G	probably benign	Het
Tpgs1	T	A	10: 79,675,510	V162D	possibly damaging	Het
Trpa1	C	T	1: 14,874,424	R1032Q	probably benign	Het
Tspan12	A	G	6: 21,795,700	Y164H	probably damaging	Het
Ubr4	T	C	4: 139,407,963	Y869H	probably damaging	Het
Vmn1r21	A	G	6: 57,843,814	I215T	probably benign	Het
Zan	A	T	5: 137,386,451	S5104T	unknown	Het
Zfp318	T	C	17: 46,412,054	V1661A	probably benign	Het
Zfp365	C	T	10: 67,888,942	R372K	probably damaging	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96739358	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96778189	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96759450	splice site	probably benign
IGL00772:Fras1	APN	5	96636112	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96534853	splice site	probably benign
IGL00913:Fras1	APN	5	96695076	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96781281	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96555221	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96673698	missense	probably null	0.58
IGL01307:Fras1	APN	5	96781692	missense	probably benign
IGL01481:Fras1	APN	5	96657241	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96739336	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96709891	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96758148	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96778045	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96588131	missense	probably benign
IGL01869:Fras1	APN	5	96708783	splice site	probably benign
IGL01923:Fras1	APN	5	96735280	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96739248	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96700523	missense	probably benign
IGL02132:Fras1	APN	5	96781637	nonsense	probably null
IGL02171:Fras1	APN	5	96735181	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96645871	nonsense	probably null
IGL02277:Fras1	APN	5	96588118	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96657408	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96769513	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96728616	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96545024	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96744705	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96691371	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96534866	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96778175	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96702181	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96710101	missense	probably benign
IGL03078:Fras1	APN	5	96636135	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96764901	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96726535	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96733781	splice site	probably benign
IGL03189:Fras1	APN	5	96743071	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96778106	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96707491	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96781760	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96733944	splice site	probably benign
IGL03394:Fras1	APN	5	96667477	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96728581	missense	probably damaging	0.99
baby_ruth	UTSW	5	96708758	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96740829	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96555301	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96677316	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96776622	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96776622	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96614917	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96710077	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96776634	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96737009	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96596888	missense	probably benign
R0352:Fras1	UTSW	5	96726540	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96762590	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96555331	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96755509	nonsense	probably null
R0395:Fras1	UTSW	5	96769653	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96691372	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96762665	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96554788	missense	probably damaging	1.00
R0456:Fras1	UTSW	5	96714343	splice site	probably null
R0464:Fras1	UTSW	5	96636803	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96700488	splice site	probably benign
R0652:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96667387	splice site	probably benign
R0765:Fras1	UTSW	5	96552796	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96768430	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96752998	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96752998	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96726543	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96714463	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96708671	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96642629	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96707308	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96707308	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96600733	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96528586	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96743187	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96636819	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96713996	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96743062	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96709978	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96713990	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96700586	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96726613	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96726613	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96598909	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96598909	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96645873	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96600784	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96552725	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96554889	splice site	probably null
R1806:Fras1	UTSW	5	96713970	splice site	probably benign
R1806:Fras1	UTSW	5	96764976	missense	possibly damaging	0.88
R1822:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96749423	splice site	probably null
R1929:Fras1	UTSW	5	96667437	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96712383	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96781203	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96654305	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96700268	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96733915	missense	probably benign
R2914:Fras1	UTSW	5	96733915	missense	probably benign
R3054:Fras1	UTSW	5	96764943	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96771712	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96771712	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96781512	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96645970	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96645970	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96733932	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96677385	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96770683	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96770683	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96743158	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96770653	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96770653	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96776735	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96714376	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96614904	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96700242	missense	probably benign
R4401:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96781348	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96709895	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96781289	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96776734	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96778088	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96700568	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96735238	missense	probably damaging	0.96
R4735:Fras1	UTSW	5	96588163	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96781659	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96731618	missense	probably benign
R4820:Fras1	UTSW	5	96728653	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96544992	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96778159	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96708758	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96636840	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96776724	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96647498	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96726580	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96650682	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96645110	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96708757	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96636173	nonsense	probably null
R5214:Fras1	UTSW	5	96769593	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96768363	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96600750	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96657250	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96741025	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96735187	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96657266	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96645160	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96780053	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96528535	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96677377	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96737021	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96728535	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96781344	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96781737	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96768381	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96709916	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96731689	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96645948	missense	probably benign
R6052:Fras1	UTSW	5	96764866	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96709985	missense	probably benign
R6256:Fras1	UTSW	5	96733843	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96764946	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96759564	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96758094	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96735202	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96598941	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96598941	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96726973	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96728551	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96543306	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96682378	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96768352	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96614873	nonsense	probably null
R7023:Fras1	UTSW	5	96710084	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96708676	missense	probably benign
R7102:Fras1	UTSW	5	96571041	missense	probably benign
R7120:Fras1	UTSW	5	96752960	nonsense	probably null
R7124:Fras1	UTSW	5	96714401	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96781284	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96778078	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96755577	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96636776	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96614912	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96739314	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96636186	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96636809	missense	probably benign	0.00
R7320:Fras1	UTSW	5	96709886	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96736970	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96596785	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96712450	nonsense	probably null
R7412:Fras1	UTSW	5	96614889	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96673599	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96768438	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96740854	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96543314	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96684437	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96684436	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96692624	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96645103	nonsense	probably null
R7722:Fras1	UTSW	5	96769554	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96712451	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96726895	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96752904	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96739318	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96762677	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96744774	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96694919	missense	probably benign
R8117:Fras1	UTSW	5	96707386	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96554855	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96588191	missense	probably benign
R8315:Fras1	UTSW	5	96743182	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96596852	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96709966	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96691401	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96755377	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96781348	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96769606	missense
R8871:Fras1	UTSW	5	96707398	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96759543	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96781279	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96567996	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96752915	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96769507	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96712556	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96636064	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96740750	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96726883	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96735163	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96657228	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96545045	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96684361	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96762500	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96534892	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96736954	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96776698	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96781392	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96753014	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96739265	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96780070	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96737036	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96740692	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96762528	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96781686	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96569494	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96743211	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96758142	missense	probably benign
Z1176:Fras1	UTSW	5	96758142	missense	probably benign
Z1177:Fras1	UTSW	5	96691457	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96700251	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96740811	missense	probably benign	0.18
Z1177:Fras1	UTSW	5	96740983	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96758142	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CATGGAGTGGGGTTGAATCC -3'
(R):5'- TCCTGTGTCTACATGGAGGC -3'

Sequencing Primer
(F):5'- AGAATTTTAGTCTCAAGGGTGGACC -3'
(R):5'- TGTGTCTACATGGAGGCCATCC -3'

Posted On

2014-06-23