Mutagenetix > Incidental Mutation

Incidental Mutation 'R1806:Vmn2r3'

203442

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

039835-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1806 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64258961-64287495 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 64275472 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 269 (M269L)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably benign
Transcript: ENSMUST00000170244
AA Change: M241L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: M241L

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000176328
AA Change: M269L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: M269L

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.5%
3x: 96.8%
10x: 94.9%
20x: 91.2%

Validation Efficiency

96% (77/80)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
8430408G22Rik	G	A	6: 116,651,722	V9M	possibly damaging	Het
Adamts15	C	A	9: 30,904,815	C616F	probably damaging	Het
Adarb1	T	C	10: 77,322,265	N116S	probably damaging	Het
Add2	C	T	6: 86,118,657	S437L	probably damaging	Het
Adra1d	T	A	2: 131,546,149	R495S	probably benign	Het
Agk	C	T	6: 40,387,495	T309I	probably damaging	Het
Aqr	T	C	2: 114,161,652	Y81C	probably damaging	Het
Bak1	G	A	17: 27,021,268	Q142*	probably null	Het
Bckdha	A	G	7: 25,631,420	V307A	probably damaging	Het
Camk2n2	C	A	16: 20,620,198	G72V	probably benign	Het
Cd276	A	T	9: 58,527,562		probably benign	Het
Cd2ap	G	A	17: 42,838,758	Q122*	probably null	Het
Cdan1	T	A	2: 120,731,426		probably benign	Het
Cdh3	T	C	8: 106,536,915	S156P	probably benign	Het
Chil4	T	A	3: 106,210,643		probably benign	Het
Col11a1	C	T	3: 114,158,142	R1074C	probably damaging	Het
Fcrlb	T	C	1: 170,907,527	T344A	probably benign	Het
Fras1	T	A	5: 96,713,970		probably benign	Het
Fras1	G	T	5: 96,764,976	V3380F	possibly damaging	Het
Galnt9	A	G	5: 110,619,253	D530G	possibly damaging	Het
Gja10	A	T	4: 32,601,135	S416R	probably benign	Het
Gm10549	T	A	18: 33,470,788	V108E	unknown	Het
Gm8298	T	C	3: 59,877,150	L348P	probably damaging	Het
Hook3	A	T	8: 26,068,659	L59Q	probably damaging	Het
Hpf1	T	A	8: 60,900,120	D178E	probably benign	Het
Hsd17b7	T	C	1: 169,961,129	N173S	possibly damaging	Het
Hsph1	A	G	5: 149,629,989	F236L	probably damaging	Het
Kcnk12	G	T	17: 87,746,109	T375K	probably benign	Het
Kdelc2	T	C	9: 53,395,850	Y365H	probably damaging	Het
Klra3	A	T	6: 130,327,070	S220T	probably damaging	Het
Lhx1	A	T	11: 84,524,141	L12Q	probably damaging	Het
Lnx1	A	G	5: 74,606,049	L468P	probably damaging	Het
Ltbp3	T	A	19: 5,753,942	C827*	probably null	Het
Mical1	C	T	10: 41,478,214	A53V	probably damaging	Het
Mmp10	A	T	9: 7,506,501	H326L	probably benign	Het
Mpl	A	T	4: 118,443,532	M600K	possibly damaging	Het
Muc5b	T	A	7: 141,865,493	D4004E	possibly damaging	Het
Myo5b	A	T	18: 74,577,609	H98L	possibly damaging	Het
Nbeal1	A	G	1: 60,284,092	T2110A	probably damaging	Het
Nedd4l	C	A	18: 65,212,791	R825S	probably damaging	Het
Ntn4	C	T	10: 93,707,353	R314W	probably damaging	Het
Olfr1277	A	T	2: 111,270,277	I30N	possibly damaging	Het
Olfr228	A	T	2: 86,483,139	I201N	probably damaging	Het
Olfr596	T	A	7: 103,310,225	L168Q	probably damaging	Het
Otog	A	T	7: 46,290,937		probably null	Het
Parp2	T	A	14: 50,819,379	L320H	probably damaging	Het
Pola2	C	T	19: 5,943,222		probably null	Het
Poln	A	T	5: 34,107,150		probably benign	Het
Pomt1	T	A	2: 32,241,668	V123E	probably damaging	Het
Prom2	T	C	2: 127,532,882	Y578C	probably damaging	Het
Prss23	T	C	7: 89,510,391	T157A	probably damaging	Het
Sdk1	T	A	5: 141,613,195	V205E	probably damaging	Het
Sdk1	A	G	5: 142,161,926	K1771R	probably benign	Het
Sidt1	A	T	16: 44,281,871	S309T	possibly damaging	Het
Sirpa	T	A	2: 129,615,512	F169I	probably damaging	Het
Slc8a1	T	C	17: 81,648,487	N374S	probably damaging	Het
Sp110	C	T	1: 85,596,110		probably null	Het
Stard9	A	G	2: 120,679,453		probably null	Het
Synpr	A	G	14: 13,563,082	N105S	probably damaging	Het
Tbc1d16	T	C	11: 119,156,101	Y440C	probably damaging	Het
Trabd	A	G	15: 89,085,621	I313V	possibly damaging	Het
Trappc10	T	C	10: 78,210,776	R430G	probably damaging	Het
Trim50	A	G	5: 135,358,889	E145G	probably benign	Het
Uba2	A	T	7: 34,163,199	F105I	probably damaging	Het
Uba3	A	G	6: 97,199,269	V92A	possibly damaging	Het
Uhmk1	T	C	1: 170,211,059	K153R	probably damaging	Het
Xpot	G	T	10: 121,607,638		probably benign	Het
Zfp128	A	G	7: 12,891,022	Y439C	probably benign	Het
Zfy1	T	A	Y: 725,620	H715L	possibly damaging	Het
Zmym1	A	C	4: 127,048,079	L839V	probably damaging	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64259883	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64274961	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64275055	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64271199	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64287395	missense	probably benign
IGL02719:Vmn2r3	APN	3	64275610	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64259818	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64278835	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64275346	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64275366	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64275633	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64275276	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64275117	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64287389	missense	possibly damaging	0.73
R1852:Vmn2r3	UTSW	3	64259394	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64259116	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64275072	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64259062	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64275312	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64275717	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64287429	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64275271	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64275601	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64259761	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64259197	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64271353	missense	probably benign
R5033:Vmn2r3	UTSW	3	64259799	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64259319	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64278826	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64259557	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64259023	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64259647	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64278761	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64275096	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64275618	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64274906	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64259506	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64259247	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64287311	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64259943	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64261404	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64275532	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64275349	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64275518	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64278814	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64275450	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64271293	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64274926	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64271196	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64278949	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64274890	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64259475	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64274962	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64287471	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64259274	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64259252	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64261382	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64278982	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64271314	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64271303	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64271248	missense	possibly damaging	0.62
X0022:Vmn2r3	UTSW	3	64274968	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTTGGGATTGCAAACCCAATAG -3'
(R):5'- GTTTCAGACTGAGGCAATTGTG -3'

Sequencing Primer
(F):5'- TAGGGGAAATACTCAGGCTTTGC -3'
(R):5'- TCAGACTGAGGCAATTGTGAATCTG -3'

Posted On

2014-06-23