Incidental Mutation 'R1860:Or4n4'
ID 203839
Institutional Source Beutler Lab
Gene Symbol Or4n4
Ensembl Gene ENSMUSG00000091873
Gene Name olfactory receptor family 4 subfamily N member 4
Synonyms GA_x6K02T2PMLR-5975274-5974348, Olfr732, MOR241-1
MMRRC Submission 039883-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.103) question?
Stock # R1860 (G1)
Quality Score 225
Status Not validated
Chromosome 14
Chromosomal Location 50518685-50519728 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) A to T at 50518848 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Stop codon at position 287 (Y287*)
Ref Sequence ENSEMBL: ENSMUSP00000148984 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071208] [ENSMUST00000213701]
AlphaFold Q8VFT5
Predicted Effect probably null
Transcript: ENSMUST00000071208
AA Change: Y287*
SMART Domains Protein: ENSMUSP00000071195
Gene: ENSMUSG00000091873
AA Change: Y287*

DomainStartEndE-ValueType
Pfam:7tm_4 30 305 7.4e-45 PFAM
Pfam:7tm_1 41 288 4.5e-21 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000213701
AA Change: Y287*
Coding Region Coverage
  • 1x: 97.4%
  • 3x: 96.8%
  • 10x: 95.1%
  • 20x: 91.7%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 83 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik A G 5: 109,884,098 (GRCm39) C587R probably damaging Het
Abca16 T A 7: 120,133,986 (GRCm39) S1320T probably benign Het
Abra C A 15: 41,732,430 (GRCm39) R212L probably damaging Het
Acadsb T A 7: 131,045,958 (GRCm39) probably null Het
Adam26a A G 8: 44,022,578 (GRCm39) V304A possibly damaging Het
Adgre1 T C 17: 57,748,363 (GRCm39) V521A probably benign Het
Adh5 G A 3: 138,159,539 (GRCm39) V288I probably benign Het
Astn1 A C 1: 158,429,515 (GRCm39) N753T probably damaging Het
Atad2 A T 15: 57,960,114 (GRCm39) probably null Het
Best3 A G 10: 116,829,178 (GRCm39) T153A probably damaging Het
Ccdc122 G A 14: 77,348,847 (GRCm39) V226I probably damaging Het
Ccdc175 T C 12: 72,152,700 (GRCm39) Q735R probably benign Het
Cd19 T A 7: 126,008,813 (GRCm39) I499F probably damaging Het
Cftr T A 6: 18,268,288 (GRCm39) L749H probably benign Het
Clec2h G T 6: 128,652,790 (GRCm39) G186W probably damaging Het
Cpsf3 T A 12: 21,346,733 (GRCm39) I202N probably damaging Het
Crebbp G T 16: 3,905,600 (GRCm39) T1669N possibly damaging Het
Csmd3 A T 15: 47,522,588 (GRCm39) C2694S probably damaging Het
Cul4a G A 8: 13,173,565 (GRCm39) R204Q probably damaging Het
Derl2 A G 11: 70,909,169 (GRCm39) F43S probably damaging Het
Dnah14 T A 1: 181,591,525 (GRCm39) N3348K probably damaging Het
Dnhd1 T C 7: 105,353,412 (GRCm39) V2855A probably benign Het
Dpysl4 G T 7: 138,670,215 (GRCm39) C27F probably benign Het
Fam228b G A 12: 4,798,314 (GRCm39) A163V probably damaging Het
Fscn1 T C 5: 142,955,818 (GRCm39) probably null Het
Fzd5 C A 1: 64,774,153 (GRCm39) R536L probably damaging Het
Gm8180 T A 14: 44,021,196 (GRCm39) H4L probably benign Het
Gpr176 T A 2: 118,203,659 (GRCm39) N4Y probably damaging Het
Grin3a T C 4: 49,665,309 (GRCm39) I1109V possibly damaging Het
Hcar1 T A 5: 124,017,092 (GRCm39) I200F probably damaging Het
Heatr4 G A 12: 84,026,502 (GRCm39) Q252* probably null Het
Hps1 G A 19: 42,750,888 (GRCm39) H371Y probably damaging Het
Ikzf2 T C 1: 69,609,661 (GRCm39) T195A probably damaging Het
Kdm1b G A 13: 47,202,666 (GRCm39) A34T probably benign Het
Lef1 A G 3: 130,905,290 (GRCm39) N57S probably damaging Het
Ltn1 A T 16: 87,213,231 (GRCm39) D443E probably benign Het
Macroh2a1 A G 13: 56,231,017 (GRCm39) L287P probably damaging Het
Mamdc2 T C 19: 23,336,517 (GRCm39) T331A probably damaging Het
Marchf10 A T 11: 105,287,904 (GRCm39) S133T probably damaging Het
Mb A G 15: 76,901,784 (GRCm39) Y104H probably damaging Het
Mrc1 C A 2: 14,333,390 (GRCm39) P1357Q probably benign Het
Nfib C T 4: 82,241,917 (GRCm39) V425M probably damaging Het
Or12j2 G A 7: 139,916,132 (GRCm39) R119H possibly damaging Het
Or4a27 T G 2: 88,559,674 (GRCm39) I90L probably damaging Het
Or4b1b C A 2: 90,112,502 (GRCm39) C139F probably damaging Het
Or4c127 G A 2: 89,833,490 (GRCm39) V247I probably benign Het
Or52u1 T C 7: 104,237,112 (GRCm39) S34P probably damaging Het
Or5b113 T A 19: 13,342,705 (GRCm39) S238T possibly damaging Het
Or5p53 A G 7: 107,533,597 (GRCm39) Y290C probably damaging Het
Or6c208 T C 10: 129,223,955 (GRCm39) F151S probably damaging Het
Or8b51 A T 9: 38,569,661 (GRCm39) V9E probably damaging Het
Phaf1 A G 8: 105,966,703 (GRCm39) E150G probably null Het
Piezo1 T C 8: 123,222,489 (GRCm39) N919S possibly damaging Het
Prkaca T C 8: 84,707,852 (GRCm39) S46P probably benign Het
Prkcb T G 7: 122,167,424 (GRCm39) V378G probably damaging Het
Ptprf A T 4: 118,081,129 (GRCm39) L576Q probably damaging Het
Rapgef4 T C 2: 72,065,064 (GRCm39) V687A probably benign Het
Rsad2 C T 12: 26,500,616 (GRCm39) V224I probably damaging Het
Ryr1 A T 7: 28,708,977 (GRCm39) D4796E unknown Het
Scn1a C T 2: 66,148,326 (GRCm39) S1073N probably damaging Het
Slc26a3 A G 12: 31,515,845 (GRCm39) M582V probably benign Het
Tbc1d32 A T 10: 55,999,633 (GRCm39) Y846* probably null Het
Tbk1 A T 10: 121,383,076 (GRCm39) M719K probably benign Het
Timeless A G 10: 128,081,983 (GRCm39) K536R probably benign Het
Tle6 G T 10: 81,430,163 (GRCm39) Q330K probably damaging Het
Tmem208 A G 8: 106,061,438 (GRCm39) K155E possibly damaging Het
Toe1 T C 4: 116,662,426 (GRCm39) Y273C probably damaging Het
Tppp2 A C 14: 52,158,062 (GRCm39) N169T probably benign Het
Ttll6 T A 11: 96,029,700 (GRCm39) Y204* probably null Het
Ubxn11 G A 4: 133,852,149 (GRCm39) S32N probably damaging Het
Usp8 G A 2: 126,597,960 (GRCm39) C961Y probably damaging Het
Vdac3 A T 8: 23,070,515 (GRCm39) I132K possibly damaging Het
Vmn2r124 G T 17: 18,269,759 (GRCm39) W5L probably benign Het
Vmn2r71 A T 7: 85,264,782 (GRCm39) D38V probably damaging Het
Vmn2r87 C T 10: 130,315,755 (GRCm39) V104I probably benign Het
Vmn2r97 C T 17: 19,167,648 (GRCm39) T634I probably benign Het
Vps50 T C 6: 3,520,279 (GRCm39) probably null Het
Wwc2 T C 8: 48,443,137 (GRCm39) N32S possibly damaging Het
Ythdc2 T A 18: 45,006,023 (GRCm39) I1172K possibly damaging Het
Zfp14 C T 7: 29,738,116 (GRCm39) V290M probably damaging Het
Zfp317 G A 9: 19,553,280 (GRCm39) A18T possibly damaging Het
Zfp354b A T 11: 50,814,369 (GRCm39) N185K probably benign Het
Zscan21 T A 5: 138,124,892 (GRCm39) D269E probably benign Het
Other mutations in Or4n4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01370:Or4n4 APN 14 50,519,689 (GRCm39) missense probably damaging 0.99
IGL01801:Or4n4 APN 14 50,519,665 (GRCm39) missense probably benign
IGL01992:Or4n4 APN 14 50,518,798 (GRCm39) missense probably benign 0.09
IGL02137:Or4n4 APN 14 50,519,135 (GRCm39) missense probably benign 0.16
IGL02494:Or4n4 APN 14 50,519,683 (GRCm39) missense probably damaging 1.00
IGL02606:Or4n4 APN 14 50,519,530 (GRCm39) missense probably damaging 1.00
IGL02799:Or4n4 UTSW 14 50,518,801 (GRCm39) missense probably benign
PIT4142001:Or4n4 UTSW 14 50,518,784 (GRCm39) makesense probably null
R0570:Or4n4 UTSW 14 50,519,370 (GRCm39) missense probably benign 0.00
R1570:Or4n4 UTSW 14 50,518,981 (GRCm39) missense probably damaging 1.00
R2313:Or4n4 UTSW 14 50,519,431 (GRCm39) missense probably damaging 1.00
R4594:Or4n4 UTSW 14 50,519,140 (GRCm39) missense probably benign 0.02
R6442:Or4n4 UTSW 14 50,518,826 (GRCm39) missense probably damaging 0.98
R6783:Or4n4 UTSW 14 50,519,644 (GRCm39) missense probably benign
R7334:Or4n4 UTSW 14 50,519,036 (GRCm39) missense probably benign 0.08
R7763:Or4n4 UTSW 14 50,518,945 (GRCm39) missense probably damaging 1.00
R8207:Or4n4 UTSW 14 50,519,036 (GRCm39) missense probably benign 0.02
R8228:Or4n4 UTSW 14 50,518,997 (GRCm39) missense probably damaging 1.00
R8806:Or4n4 UTSW 14 50,519,236 (GRCm39) missense probably benign 0.04
R8809:Or4n4 UTSW 14 50,519,236 (GRCm39) missense probably benign 0.04
R8898:Or4n4 UTSW 14 50,518,952 (GRCm39) missense probably damaging 1.00
R8962:Or4n4 UTSW 14 50,518,816 (GRCm39) missense possibly damaging 0.95
R9800:Or4n4 UTSW 14 50,519,701 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- AACTGTGTCAAATTGCCTATATCCTTC -3'
(R):5'- CAATAGTGGCCTGCTTACCC -3'

Sequencing Primer
(F):5'- GCCTATATCCTTCCTGAAAAATGAG -3'
(R):5'- ATGCCATGTTCACAGGTCAG -3'
Posted On 2014-06-23