Mutagenetix > Incidental Mutation

Incidental Mutation 'R1833:Mdn1'

204932

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, LOC213784, D4Abb1e

MMRRC Submission

039860-MU

Accession Numbers

Genbank: NM_001081392; MGI: 1926159

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1833 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 32720761 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 2291 (H2291Q)

Ref Sequence

ENSEMBL: ENSMUSP00000136222 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000178134]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000071642
AA Change: H2291Q

PolyPhen 2 Score 0.890 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006
AA Change: H2291Q

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125323

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150934

Predicted Effect

probably damaging
Transcript: ENSMUST00000178134
AA Change: H2291Q

PolyPhen 2 Score 0.968 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006
AA Change: H2291Q

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Meta Mutation Damage Score

0.1182

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.9%

Validation Efficiency

99% (83/84)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003H04Rik	T	A	3: 124,556,860 (GRCm38)	D143V	unknown	Het
4930438A08Rik	C	T	11: 58,288,388 (GRCm38)	Q183*	probably null	Het
9230009I02Rik	A	G	11: 51,091,466 (GRCm38)		noncoding transcript	Het
Abhd12	T	A	2: 150,848,418 (GRCm38)	D119V	probably damaging	Het
Adam1b	A	G	5: 121,502,937 (GRCm38)	I15T	possibly damaging	Het
Agtpbp1	A	T	13: 59,465,983 (GRCm38)		probably null	Het
Arfgef1	G	C	1: 10,204,890 (GRCm38)	I312M	probably benign	Het
Arid4a	C	T	12: 71,075,466 (GRCm38)	L874F	possibly damaging	Het
Bcas3	G	A	11: 85,583,949 (GRCm38)	V317I	probably benign	Het
Ccr1	T	A	9: 123,964,089 (GRCm38)	I135F	probably damaging	Het
Ces2h	A	G	8: 105,020,373 (GRCm38)	E547G	possibly damaging	Het
Ces3b	T	A	8: 105,085,639 (GRCm38)	D173E	probably damaging	Het
Chd3	A	G	11: 69,354,123 (GRCm38)	L1197S	probably damaging	Het
Cngb1	A	G	8: 95,242,355 (GRCm38)	L1175P	probably damaging	Het
Cyp4f17	T	C	17: 32,524,210 (GRCm38)	F286L	probably benign	Het
Dclre1a	C	T	19: 56,541,500 (GRCm38)		probably null	Het
Dennd6a	T	A	14: 26,606,954 (GRCm38)	L44H	probably damaging	Het
Dhx16	A	G	17: 35,885,619 (GRCm38)	T560A	probably benign	Het
Dusp12	T	C	1: 170,874,453 (GRCm38)	M326V	probably benign	Het
Eif3k	T	C	7: 28,971,427 (GRCm38)	I180V	probably benign	Het
Erc1	A	G	6: 119,743,429 (GRCm38)	I437T	possibly damaging	Het
Fam71d	G	A	12: 78,715,506 (GRCm38)		probably benign	Het
Farp2	T	A	1: 93,576,364 (GRCm38)		probably benign	Het
Foxa3	A	G	7: 19,014,574 (GRCm38)	L209P	probably damaging	Het
Gen1	A	T	12: 11,248,351 (GRCm38)		probably benign	Het
Gm10305	A	G	4: 99,273,126 (GRCm38)	T91A	unknown	Het
Gm10436	T	C	12: 88,178,448 (GRCm38)	E44G	possibly damaging	Het
Gm14412	T	C	2: 177,315,790 (GRCm38)	D104G	probably benign	Het
Gm340	T	C	19: 41,584,948 (GRCm38)	I714T	probably benign	Het
Gm6900	T	C	7: 10,656,588 (GRCm38)		noncoding transcript	Het
Gpx1	A	T	9: 108,339,356 (GRCm38)	Y15F	possibly damaging	Het
H2-M10.3	T	C	17: 36,367,495 (GRCm38)	Y146C	probably damaging	Het
H2-Q7	C	A	17: 35,439,699 (GRCm38)	S104R	probably benign	Het
Hephl1	T	C	9: 15,076,928 (GRCm38)	Y628C	probably damaging	Het
Hspa5	T	A	2: 34,776,053 (GRCm38)	Y636*	probably null	Het
Htt	A	G	5: 34,905,748 (GRCm38)		probably benign	Het
Idh1	T	C	1: 65,161,114 (GRCm38)	I364V	probably benign	Het
Itgae	G	T	11: 73,117,162 (GRCm38)	A423S	possibly damaging	Het
Kng2	T	C	16: 23,012,052 (GRCm38)	N169S	possibly damaging	Het
Larp4b	C	T	13: 9,151,199 (GRCm38)	T369I	possibly damaging	Het
Lonrf2	G	A	1: 38,813,276 (GRCm38)	P165S	probably benign	Het
Magi2	G	T	5: 19,227,457 (GRCm38)	G57C	probably damaging	Het
Mgam	T	C	6: 40,654,718 (GRCm38)		probably null	Het
Micall2	A	G	5: 139,716,753 (GRCm38)	V245A	probably benign	Het
Mipep	T	G	14: 60,872,063 (GRCm38)	Y630D	probably damaging	Het
Msx2	A	T	13: 53,468,185 (GRCm38)	M263K	probably damaging	Het
Nectin2	G	T	7: 19,717,708 (GRCm38)	P467H	probably damaging	Het
Nek10	A	T	14: 14,842,789 (GRCm38)	M165L	probably benign	Het
Nlrp4b	A	T	7: 10,725,936 (GRCm38)	M455L	probably benign	Het
Oaz3	TGGAGGCAGGAGCACGGGAGGCAGGAGCACGGGAGGCAG	TGGAGGCAGGAGCACGGGAGGCAG	3: 94,436,042 (GRCm38)		probably benign	Het
Olfr1258	T	A	2: 89,930,301 (GRCm38)	L164*	probably null	Het
Olfr286	T	A	15: 98,226,965 (GRCm38)	I227F	probably damaging	Het
Pcx	T	A	19: 4,619,104 (GRCm38)	V710E	probably damaging	Het
Pkn2	C	T	3: 142,821,647 (GRCm38)	R347Q	probably damaging	Het
Qsox1	C	T	1: 155,791,045 (GRCm38)	G233S	probably benign	Het
Rbl1	T	A	2: 157,195,555 (GRCm38)	N224I	probably damaging	Het
Rspry1	A	G	8: 94,635,488 (GRCm38)	T132A	probably damaging	Het
Sclt1	A	G	3: 41,727,111 (GRCm38)	V91A	probably damaging	Het
Sema4f	A	T	6: 82,918,559 (GRCm38)	L331H	probably benign	Het
Sf3b3	T	A	8: 110,817,566 (GRCm38)	Q814L	probably benign	Het
Slc19a2	T	G	1: 164,262,184 (GRCm38)	Y190D	probably damaging	Het
Smarcc1	T	A	9: 110,153,811 (GRCm38)	H204Q	possibly damaging	Het
Sox6	T	C	7: 115,777,093 (GRCm38)	K135E	probably damaging	Het
Tecpr1	T	C	5: 144,208,608 (GRCm38)	Q607R	probably damaging	Het
Tgfb1i1	A	G	7: 128,249,498 (GRCm38)		probably benign	Het
Tirap	T	G	9: 35,188,703 (GRCm38)	R228S	probably benign	Het
Tmem211	A	G	5: 113,234,569 (GRCm38)		probably benign	Het
Trp53bp2	T	A	1: 182,429,016 (GRCm38)	H50Q	probably damaging	Het
Try4	A	G	6: 41,303,431 (GRCm38)	H63R	probably damaging	Het
Vmn2r25	G	A	6: 123,839,684 (GRCm38)	P313S	probably benign	Het
Vps26a	A	C	10: 62,459,046 (GRCm38)	L250V	probably benign	Het
Vwf	A	G	6: 125,642,037 (GRCm38)	H1226R	probably benign	Het
Wdtc1	TCC	TC	4: 133,308,742 (GRCm38)		probably benign	Het
Zc3hav1l	A	T	6: 38,297,946 (GRCm38)		probably benign	Het
Zfp119b	T	G	17: 55,939,271 (GRCm38)	H305P	probably damaging	Het
Zfp326	C	T	5: 105,891,169 (GRCm38)	Q134*	probably null	Het
Zfp975	C	T	7: 42,661,839 (GRCm38)	R450Q	probably benign	Het
Zfyve26	A	T	12: 79,286,258 (GRCm38)	M313K	probably benign	Het
Zscan5b	T	C	7: 6,238,966 (GRCm38)	S395P	possibly damaging	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32,723,651 (GRCm38)	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32,719,214 (GRCm38)	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32,735,719 (GRCm38)	missense	probably benign
IGL00573:Mdn1	APN	4	32,666,619 (GRCm38)	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32,735,525 (GRCm38)	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32,730,864 (GRCm38)	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32,743,686 (GRCm38)	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32,715,922 (GRCm38)	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32,711,938 (GRCm38)	splice site	probably benign
IGL01684:Mdn1	APN	4	32,726,857 (GRCm38)	missense	probably benign
IGL01753:Mdn1	APN	4	32,708,483 (GRCm38)	missense	probably benign
IGL01901:Mdn1	APN	4	32,669,591 (GRCm38)	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32,723,657 (GRCm38)	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32,758,393 (GRCm38)	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32,749,948 (GRCm38)	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32,715,708 (GRCm38)	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32,709,364 (GRCm38)	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32,740,395 (GRCm38)	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32,739,092 (GRCm38)	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32,676,860 (GRCm38)	splice site	probably benign
IGL02396:Mdn1	APN	4	32,700,120 (GRCm38)	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32,694,674 (GRCm38)	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32,670,579 (GRCm38)	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32,763,199 (GRCm38)	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32,734,366 (GRCm38)	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32,713,360 (GRCm38)	splice site	probably benign
IGL03076:Mdn1	APN	4	32,735,564 (GRCm38)	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32,729,994 (GRCm38)	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32,732,842 (GRCm38)	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32,725,967 (GRCm38)	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32,694,495 (GRCm38)	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32,749,934 (GRCm38)	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32,719,223 (GRCm38)	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32,746,527 (GRCm38)	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32,718,317 (GRCm38)	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32,729,956 (GRCm38)	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32,693,534 (GRCm38)	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32,741,835 (GRCm38)	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32,750,318 (GRCm38)	missense	probably damaging	1.00
R0362:Mdn1	UTSW	4	32,746,439 (GRCm38)	critical splice acceptor site	probably null
R0421:Mdn1	UTSW	4	32,684,707 (GRCm38)	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32,699,204 (GRCm38)	splice site	probably benign
R0469:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0477:Mdn1	UTSW	4	32,750,928 (GRCm38)	missense	probably benign	0.02
R0481:Mdn1	UTSW	4	32,767,182 (GRCm38)	splice site	probably benign
R0504:Mdn1	UTSW	4	32,698,916 (GRCm38)	splice site	probably benign
R0522:Mdn1	UTSW	4	32,672,837 (GRCm38)	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32,730,479 (GRCm38)	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32,732,829 (GRCm38)	missense	probably benign	0.36
R0607:Mdn1	UTSW	4	32,712,014 (GRCm38)	missense	probably damaging	1.00
R0664:Mdn1	UTSW	4	32,768,011 (GRCm38)	nonsense	probably null
R0701:Mdn1	UTSW	4	32,699,263 (GRCm38)	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32,668,895 (GRCm38)	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32,752,032 (GRCm38)	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32,741,835 (GRCm38)	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32,701,713 (GRCm38)	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32,746,568 (GRCm38)	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32,694,511 (GRCm38)	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32,667,089 (GRCm38)	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32,700,334 (GRCm38)	splice site	probably benign
R1466:Mdn1	UTSW	4	32,730,788 (GRCm38)	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32,730,788 (GRCm38)	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32,739,977 (GRCm38)	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32,723,501 (GRCm38)	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32,722,315 (GRCm38)	missense	probably benign
R1574:Mdn1	UTSW	4	32,722,315 (GRCm38)	missense	probably benign
R1591:Mdn1	UTSW	4	32,700,092 (GRCm38)	missense	possibly damaging	0.65
R1678:Mdn1	UTSW	4	32,663,050 (GRCm38)	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32,700,417 (GRCm38)	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32,693,504 (GRCm38)	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32,773,952 (GRCm38)	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32,700,103 (GRCm38)	missense	probably damaging	1.00
R1858:Mdn1	UTSW	4	32,730,881 (GRCm38)	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32,763,339 (GRCm38)	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32,742,540 (GRCm38)	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32,760,839 (GRCm38)	small deletion	probably benign
R2075:Mdn1	UTSW	4	32,716,058 (GRCm38)	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32,738,712 (GRCm38)	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32,743,843 (GRCm38)	splice site	probably null
R2110:Mdn1	UTSW	4	32,700,409 (GRCm38)	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32,700,409 (GRCm38)	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32,716,271 (GRCm38)	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32,763,306 (GRCm38)	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32,765,701 (GRCm38)	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32,750,010 (GRCm38)	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32,723,621 (GRCm38)	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32,750,013 (GRCm38)	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32,733,726 (GRCm38)	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32,733,726 (GRCm38)	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32,720,818 (GRCm38)	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32,693,506 (GRCm38)	nonsense	probably null
R3973:Mdn1	UTSW	4	32,722,363 (GRCm38)	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32,707,475 (GRCm38)	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32,743,809 (GRCm38)	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32,754,482 (GRCm38)	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32,704,635 (GRCm38)	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32,668,860 (GRCm38)	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32,715,883 (GRCm38)	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32,722,334 (GRCm38)	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32,754,437 (GRCm38)	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32,741,812 (GRCm38)	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32,707,636 (GRCm38)	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32,730,749 (GRCm38)	splice site	probably null
R4667:Mdn1	UTSW	4	32,679,572 (GRCm38)	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32,666,430 (GRCm38)	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32,683,583 (GRCm38)	nonsense	probably null
R4807:Mdn1	UTSW	4	32,685,651 (GRCm38)	splice site	probably null
R4923:Mdn1	UTSW	4	32,671,608 (GRCm38)	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32,707,459 (GRCm38)	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32,756,512 (GRCm38)	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32,739,827 (GRCm38)	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32,734,418 (GRCm38)	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32,670,593 (GRCm38)	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32,774,008 (GRCm38)	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32,759,011 (GRCm38)	splice site	probably null
R5215:Mdn1	UTSW	4	32,741,418 (GRCm38)	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32,720,897 (GRCm38)	missense	probably benign
R5522:Mdn1	UTSW	4	32,685,783 (GRCm38)	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32,767,961 (GRCm38)	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32,728,167 (GRCm38)	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32,765,664 (GRCm38)	missense	probably benign
R5621:Mdn1	UTSW	4	32,716,371 (GRCm38)	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32,695,480 (GRCm38)	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32,667,467 (GRCm38)	splice site	probably null
R5780:Mdn1	UTSW	4	32,722,950 (GRCm38)	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32,754,547 (GRCm38)	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32,670,646 (GRCm38)	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32,695,400 (GRCm38)	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32,678,330 (GRCm38)	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32,741,073 (GRCm38)	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32,715,713 (GRCm38)	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32,689,581 (GRCm38)	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32,684,735 (GRCm38)	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32,716,040 (GRCm38)	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32,715,953 (GRCm38)	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32,696,269 (GRCm38)	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32,708,484 (GRCm38)	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32,748,590 (GRCm38)	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32,749,593 (GRCm38)	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32,715,979 (GRCm38)	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32,730,054 (GRCm38)	nonsense	probably null
R6384:Mdn1	UTSW	4	32,670,607 (GRCm38)	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32,773,308 (GRCm38)	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32,713,780 (GRCm38)	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32,774,041 (GRCm38)	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32,676,786 (GRCm38)	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32,741,893 (GRCm38)	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32,748,614 (GRCm38)	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32,774,041 (GRCm38)	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32,726,942 (GRCm38)	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32,733,374 (GRCm38)	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32,767,969 (GRCm38)	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32,762,341 (GRCm38)	missense	probably benign
R7158:Mdn1	UTSW	4	32,725,121 (GRCm38)	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32,746,446 (GRCm38)	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32,719,184 (GRCm38)	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32,694,634 (GRCm38)	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32,701,823 (GRCm38)	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32,695,427 (GRCm38)	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32,725,944 (GRCm38)	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32,723,685 (GRCm38)	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32,691,729 (GRCm38)	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32,773,375 (GRCm38)	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32,739,030 (GRCm38)	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32,667,270 (GRCm38)	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32,696,359 (GRCm38)	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32,694,599 (GRCm38)	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32,691,229 (GRCm38)	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32,739,912 (GRCm38)	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32,741,344 (GRCm38)	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32,741,344 (GRCm38)	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32,722,360 (GRCm38)	missense	possibly damaging	0.75
R7718:Mdn1	UTSW	4	32,718,420 (GRCm38)	missense	probably damaging	1.00
R7762:Mdn1	UTSW	4	32,734,421 (GRCm38)	missense	probably benign
R7787:Mdn1	UTSW	4	32,741,794 (GRCm38)	missense	probably damaging	1.00
R8111:Mdn1	UTSW	4	32,674,003 (GRCm38)	missense	possibly damaging	0.81
R8222:Mdn1	UTSW	4	32,707,477 (GRCm38)	missense	probably benign	0.09
R8246:Mdn1	UTSW	4	32,657,284 (GRCm38)	missense	probably benign	0.06
R8267:Mdn1	UTSW	4	32,742,485 (GRCm38)	missense	possibly damaging	0.82
R8286:Mdn1	UTSW	4	32,731,960 (GRCm38)	missense	possibly damaging	0.91
R8305:Mdn1	UTSW	4	32,725,107 (GRCm38)	missense	probably benign
R8318:Mdn1	UTSW	4	32,735,897 (GRCm38)	critical splice donor site	probably null
R8379:Mdn1	UTSW	4	32,756,453 (GRCm38)	missense	probably null	1.00
R8384:Mdn1	UTSW	4	32,765,680 (GRCm38)	missense	probably benign	0.05
R8514:Mdn1	UTSW	4	32,739,857 (GRCm38)	missense	probably damaging	1.00
R8560:Mdn1	UTSW	4	32,743,830 (GRCm38)	missense	probably benign	0.08
R8672:Mdn1	UTSW	4	32,768,793 (GRCm38)	missense	probably damaging	1.00
R8708:Mdn1	UTSW	4	32,725,854 (GRCm38)	missense	probably damaging	1.00
R8769:Mdn1	UTSW	4	32,751,390 (GRCm38)	missense	probably damaging	0.97
R8896:Mdn1	UTSW	4	32,678,328 (GRCm38)	missense	probably benign	0.28
R8918:Mdn1	UTSW	4	32,744,579 (GRCm38)	nonsense	probably null
R8920:Mdn1	UTSW	4	32,719,280 (GRCm38)	missense	probably damaging	1.00
R8966:Mdn1	UTSW	4	32,672,837 (GRCm38)	nonsense	probably null
R8997:Mdn1	UTSW	4	32,773,275 (GRCm38)	missense	probably damaging	1.00
R9120:Mdn1	UTSW	4	32,701,814 (GRCm38)	missense	probably damaging	1.00
R9129:Mdn1	UTSW	4	32,676,812 (GRCm38)	missense	probably benign	0.24
R9131:Mdn1	UTSW	4	32,762,275 (GRCm38)	missense	possibly damaging	0.69
R9200:Mdn1	UTSW	4	32,760,791 (GRCm38)	missense	probably benign	0.00
R9226:Mdn1	UTSW	4	32,694,612 (GRCm38)	missense	probably benign	0.25
R9235:Mdn1	UTSW	4	32,739,122 (GRCm38)	missense	probably benign	0.10
R9293:Mdn1	UTSW	4	32,707,579 (GRCm38)	missense	probably damaging	1.00
R9315:Mdn1	UTSW	4	32,760,911 (GRCm38)	missense	probably benign	0.00
R9338:Mdn1	UTSW	4	32,666,536 (GRCm38)	missense	probably benign	0.00
R9353:Mdn1	UTSW	4	32,693,504 (GRCm38)	missense	probably damaging	1.00
R9393:Mdn1	UTSW	4	32,713,825 (GRCm38)	missense
R9420:Mdn1	UTSW	4	32,678,414 (GRCm38)	missense	probably damaging	1.00
R9475:Mdn1	UTSW	4	32,739,849 (GRCm38)	missense	possibly damaging	0.65
R9583:Mdn1	UTSW	4	32,741,372 (GRCm38)	missense	probably damaging	1.00
R9600:Mdn1	UTSW	4	32,684,723 (GRCm38)	nonsense	probably null
R9640:Mdn1	UTSW	4	32,754,539 (GRCm38)	missense	probably damaging	1.00
R9688:Mdn1	UTSW	4	32,745,590 (GRCm38)	missense	probably damaging	1.00
R9744:Mdn1	UTSW	4	32,715,711 (GRCm38)	missense	possibly damaging	0.91
X0066:Mdn1	UTSW	4	32,739,030 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,696,244 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,668,944 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,667,102 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CACACAGTGATGAAGTCAGCTTC -3'
(R):5'- GACTAAAACTGCAGAGGCCC -3'

Sequencing Primer
(F):5'- AAGTCAGCTTCTTCTGCCATGGAG -3'
(R):5'- ACCTCTCACAAGGCTCTGG -3'

Posted On

2014-06-23