Incidental Mutation 'R1834:Robo4'
| ID |
205060 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Robo4
|
| Ensembl Gene |
ENSMUSG00000032125 |
| Gene Name |
roundabout guidance receptor 4 |
| Synonyms |
Magic roundabout, 1200012D01Rik |
| MMRRC Submission |
039861-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.224)
|
| Stock # |
R1834 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
9 |
| Chromosomal Location |
37313198-37325319 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 37324355 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Aspartic acid to Glycine
at position 961
(D961G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000150722
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000034643]
[ENSMUST00000102895]
[ENSMUST00000115038]
[ENSMUST00000115046]
[ENSMUST00000115048]
[ENSMUST00000156972]
[ENSMUST00000170512]
[ENSMUST00000214185]
|
| AlphaFold |
Q8C310 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000034643
|
| SMART Domains |
Protein: ENSMUSP00000034643
Gene: ENSMUSG00000032128
| Domain | Start | End | E-Value | Type |
|---|
|
IGc2
|
54 |
128 |
9.7e-11 |
SMART |
|
IGc2
|
156 |
221 |
1.44e-4 |
SMART |
|
IGc2
|
248 |
311 |
1.89e-13 |
SMART |
|
IGc2
|
337 |
409 |
9.84e-12 |
SMART |
|
IGc2
|
441 |
506 |
2.09e-15 |
SMART |
|
FN3
|
534 |
616 |
4.24e-14 |
SMART |
|
FN3
|
648 |
731 |
3.06e0 |
SMART |
|
FN3
|
747 |
832 |
1.97e-9 |
SMART |
|
low complexity region
|
870 |
890 |
N/A |
INTRINSIC |
|
low complexity region
|
1055 |
1082 |
N/A |
INTRINSIC |
|
low complexity region
|
1131 |
1149 |
N/A |
INTRINSIC |
|
low complexity region
|
1155 |
1169 |
N/A |
INTRINSIC |
|
low complexity region
|
1193 |
1206 |
N/A |
INTRINSIC |
|
low complexity region
|
1245 |
1256 |
N/A |
INTRINSIC |
|
low complexity region
|
1268 |
1281 |
N/A |
INTRINSIC |
|
low complexity region
|
1336 |
1376 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000102895
AA Change: D954G
PolyPhen 2
Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)
|
| SMART Domains |
Protein: ENSMUSP00000099959
Gene: ENSMUSG00000032125
AA Change: D954G
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
37 |
N/A |
INTRINSIC |
|
IG
|
48 |
144 |
2.51e0 |
SMART |
|
IGc2
|
160 |
225 |
6.86e-11 |
SMART |
|
FN3
|
263 |
343 |
2.05e0 |
SMART |
|
FN3
|
358 |
440 |
1.27e-3 |
SMART |
|
low complexity region
|
488 |
494 |
N/A |
INTRINSIC |
|
low complexity region
|
544 |
562 |
N/A |
INTRINSIC |
|
low complexity region
|
720 |
733 |
N/A |
INTRINSIC |
|
low complexity region
|
748 |
762 |
N/A |
INTRINSIC |
|
low complexity region
|
775 |
799 |
N/A |
INTRINSIC |
|
low complexity region
|
855 |
866 |
N/A |
INTRINSIC |
|
low complexity region
|
871 |
880 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000115038
|
| SMART Domains |
Protein: ENSMUSP00000110690
Gene: ENSMUSG00000032128
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
34 |
47 |
N/A |
INTRINSIC |
|
IGc2
|
76 |
150 |
9.7e-11 |
SMART |
|
IGc2
|
178 |
243 |
1.44e-4 |
SMART |
|
IGc2
|
270 |
333 |
1.89e-13 |
SMART |
|
IGc2
|
359 |
431 |
9.84e-12 |
SMART |
|
IGc2
|
463 |
528 |
2.09e-15 |
SMART |
|
FN3
|
556 |
638 |
4.24e-14 |
SMART |
|
FN3
|
670 |
753 |
3.06e0 |
SMART |
|
FN3
|
769 |
854 |
1.97e-9 |
SMART |
|
low complexity region
|
892 |
912 |
N/A |
INTRINSIC |
|
low complexity region
|
1077 |
1104 |
N/A |
INTRINSIC |
|
low complexity region
|
1153 |
1171 |
N/A |
INTRINSIC |
|
low complexity region
|
1177 |
1191 |
N/A |
INTRINSIC |
|
low complexity region
|
1215 |
1228 |
N/A |
INTRINSIC |
|
low complexity region
|
1267 |
1278 |
N/A |
INTRINSIC |
|
low complexity region
|
1290 |
1303 |
N/A |
INTRINSIC |
|
low complexity region
|
1358 |
1398 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000115046
AA Change: D1013G
PolyPhen 2
Score 0.049 (Sensitivity: 0.94; Specificity: 0.83)
|
| SMART Domains |
Protein: ENSMUSP00000110698
Gene: ENSMUSG00000032125
AA Change: D1013G
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
37 |
N/A |
INTRINSIC |
|
IG
|
48 |
144 |
2.51e0 |
SMART |
|
IGc2
|
160 |
225 |
6.86e-11 |
SMART |
|
FN3
|
263 |
343 |
2.05e0 |
SMART |
|
FN3
|
358 |
440 |
1.27e-3 |
SMART |
|
low complexity region
|
484 |
500 |
N/A |
INTRINSIC |
|
low complexity region
|
540 |
546 |
N/A |
INTRINSIC |
|
low complexity region
|
596 |
614 |
N/A |
INTRINSIC |
|
low complexity region
|
747 |
756 |
N/A |
INTRINSIC |
|
low complexity region
|
779 |
792 |
N/A |
INTRINSIC |
|
low complexity region
|
807 |
821 |
N/A |
INTRINSIC |
|
low complexity region
|
834 |
858 |
N/A |
INTRINSIC |
|
low complexity region
|
914 |
925 |
N/A |
INTRINSIC |
|
low complexity region
|
930 |
939 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000115048
AA Change: D850G
PolyPhen 2
Score 0.056 (Sensitivity: 0.94; Specificity: 0.84)
|
| SMART Domains |
Protein: ENSMUSP00000110700
Gene: ENSMUSG00000032125
AA Change: D850G
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
37 |
N/A |
INTRINSIC |
|
IG
|
48 |
144 |
2.51e0 |
SMART |
|
IGc2
|
160 |
225 |
6.86e-11 |
SMART |
|
FN3
|
263 |
343 |
2.05e0 |
SMART |
|
FN3
|
358 |
440 |
1.27e-3 |
SMART |
|
low complexity region
|
488 |
494 |
N/A |
INTRINSIC |
|
low complexity region
|
544 |
562 |
N/A |
INTRINSIC |
|
low complexity region
|
695 |
704 |
N/A |
INTRINSIC |
|
low complexity region
|
727 |
740 |
N/A |
INTRINSIC |
|
low complexity region
|
755 |
769 |
N/A |
INTRINSIC |
|
low complexity region
|
782 |
806 |
N/A |
INTRINSIC |
|
low complexity region
|
862 |
873 |
N/A |
INTRINSIC |
|
low complexity region
|
878 |
887 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000156972
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000167089
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000167216
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000170512
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000171467
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000214185
AA Change: D961G
PolyPhen 2
Score 0.086 (Sensitivity: 0.93; Specificity: 0.85)
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000215777
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 97.4%
- 3x: 96.8%
- 10x: 95.1%
- 20x: 92.0%
|
| Validation Efficiency |
97% (116/119) |
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a reporter/null allele display enhanced VEGF-induced endothelial migration, tube formation and vascular permeability, and show increased pathologic angiogenesis and vascular leak in models of oxygen-induced retinopathy and choroidal neovascularization. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 114 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 2310033P09Rik |
A |
G |
11: 59,099,313 (GRCm39) |
D20G |
probably damaging |
Het |
| 2310061N02Rik |
T |
A |
16: 88,504,786 (GRCm39) |
S4C |
unknown |
Het |
| Abca3 |
A |
T |
17: 24,595,666 (GRCm39) |
N340Y |
probably benign |
Het |
| Abcc1 |
A |
G |
16: 14,240,981 (GRCm39) |
I513V |
possibly damaging |
Het |
| Acacb |
C |
T |
5: 114,373,536 (GRCm39) |
T1855M |
probably damaging |
Het |
| Ace |
A |
G |
11: 105,876,920 (GRCm39) |
|
probably benign |
Het |
| Acss2 |
T |
A |
2: 155,400,550 (GRCm39) |
Y530N |
probably damaging |
Het |
| Adarb1 |
C |
A |
10: 77,153,065 (GRCm39) |
|
probably benign |
Het |
| Afm |
T |
A |
5: 90,674,283 (GRCm39) |
M265K |
probably benign |
Het |
| Agl |
A |
G |
3: 116,582,000 (GRCm39) |
F293S |
probably benign |
Het |
| Aox1 |
G |
A |
1: 58,348,150 (GRCm39) |
A623T |
probably benign |
Het |
| Arhgap31 |
C |
A |
16: 38,424,065 (GRCm39) |
S667I |
probably benign |
Het |
| Bco1 |
A |
G |
8: 117,844,176 (GRCm39) |
T297A |
probably benign |
Het |
| Bmp1 |
T |
C |
14: 70,746,271 (GRCm39) |
S123G |
possibly damaging |
Het |
| Caps2 |
G |
A |
10: 112,031,623 (GRCm39) |
D283N |
possibly damaging |
Het |
| Ccdc57 |
A |
C |
11: 120,752,045 (GRCm39) |
S845R |
probably benign |
Het |
| Cd209f |
A |
G |
8: 4,154,491 (GRCm39) |
S119P |
probably damaging |
Het |
| Chrna6 |
A |
G |
8: 27,897,242 (GRCm39) |
S212P |
probably benign |
Het |
| Col10a1 |
G |
C |
10: 34,271,011 (GRCm39) |
A328P |
probably damaging |
Het |
| Col4a2 |
A |
G |
8: 11,452,997 (GRCm39) |
E188G |
probably benign |
Het |
| Creb1 |
C |
T |
1: 64,590,109 (GRCm39) |
Q32* |
probably null |
Het |
| Cttnbp2 |
C |
T |
6: 18,501,965 (GRCm39) |
V16M |
probably damaging |
Het |
| D430041D05Rik |
A |
G |
2: 103,998,446 (GRCm39) |
S1102P |
probably damaging |
Het |
| Ddx31 |
C |
T |
2: 28,782,465 (GRCm39) |
H603Y |
probably damaging |
Het |
| Ddx52 |
G |
A |
11: 83,850,323 (GRCm39) |
C568Y |
probably benign |
Het |
| Dgki |
C |
A |
6: 37,011,636 (GRCm39) |
|
probably benign |
Het |
| Dmrt1 |
A |
T |
19: 25,487,063 (GRCm39) |
Q123L |
probably damaging |
Het |
| Dnah5 |
A |
T |
15: 28,409,270 (GRCm39) |
M3563L |
probably benign |
Het |
| Dnah7b |
A |
T |
1: 46,272,919 (GRCm39) |
N2349I |
possibly damaging |
Het |
| Dscaml1 |
T |
G |
9: 45,594,930 (GRCm39) |
S678A |
probably benign |
Het |
| Ece1 |
C |
T |
4: 137,685,312 (GRCm39) |
R601W |
probably damaging |
Het |
| Ece1 |
A |
G |
4: 137,685,439 (GRCm39) |
N643S |
probably damaging |
Het |
| Eno1b |
A |
G |
18: 48,180,530 (GRCm39) |
Y236C |
probably damaging |
Het |
| Esco1 |
T |
C |
18: 10,594,350 (GRCm39) |
E312G |
probably damaging |
Het |
| Eya3 |
T |
A |
4: 132,434,429 (GRCm39) |
V276E |
probably damaging |
Het |
| Fam161b |
A |
G |
12: 84,395,552 (GRCm39) |
|
probably benign |
Het |
| Fem1c |
C |
T |
18: 46,638,349 (GRCm39) |
G551D |
probably damaging |
Het |
| Fer1l6 |
T |
C |
15: 58,429,718 (GRCm39) |
I155T |
possibly damaging |
Het |
| Gbp7 |
A |
G |
3: 142,240,441 (GRCm39) |
Y53C |
probably damaging |
Het |
| Gjb3 |
G |
A |
4: 127,220,224 (GRCm39) |
R103W |
probably damaging |
Het |
| Gm10030 |
C |
T |
9: 110,833,947 (GRCm39) |
|
noncoding transcript |
Het |
| Gm3443 |
T |
A |
19: 21,533,043 (GRCm39) |
H2Q |
unknown |
Het |
| Gm5800 |
T |
C |
14: 51,953,549 (GRCm39) |
E22G |
possibly damaging |
Het |
| Gsdma2 |
G |
T |
11: 98,539,905 (GRCm39) |
R9L |
probably damaging |
Het |
| Gvin3 |
A |
T |
7: 106,202,983 (GRCm39) |
V87D |
possibly damaging |
Het |
| Ica1l |
A |
G |
1: 60,067,395 (GRCm39) |
|
probably benign |
Het |
| Ice1 |
G |
A |
13: 70,763,457 (GRCm39) |
T167I |
probably damaging |
Het |
| Ifi204 |
T |
A |
1: 173,575,172 (GRCm39) |
R618S |
unknown |
Het |
| Itga2 |
C |
A |
13: 114,993,262 (GRCm39) |
K784N |
probably damaging |
Het |
| Itga2 |
T |
A |
13: 114,993,263 (GRCm39) |
K784M |
probably damaging |
Het |
| Kif3b |
A |
G |
2: 153,159,405 (GRCm39) |
E402G |
probably benign |
Het |
| Lrp2 |
T |
C |
2: 69,297,224 (GRCm39) |
I3246V |
probably benign |
Het |
| Lrrn3 |
G |
T |
12: 41,503,517 (GRCm39) |
L267I |
probably damaging |
Het |
| Map1s |
A |
G |
8: 71,369,055 (GRCm39) |
Y868C |
probably damaging |
Het |
| Mcm5 |
A |
G |
8: 75,845,901 (GRCm39) |
T370A |
possibly damaging |
Het |
| Nat8f7 |
T |
C |
6: 85,684,793 (GRCm39) |
S16G |
probably benign |
Het |
| Nav3 |
T |
C |
10: 109,555,883 (GRCm39) |
T1683A |
probably damaging |
Het |
| Nbeal2 |
T |
C |
9: 110,456,197 (GRCm39) |
N2417S |
probably damaging |
Het |
| Neb |
T |
C |
2: 52,126,907 (GRCm39) |
N3605S |
probably damaging |
Het |
| Net1 |
A |
T |
13: 3,962,941 (GRCm39) |
|
probably benign |
Het |
| Nsd1 |
A |
G |
13: 55,461,164 (GRCm39) |
T2464A |
possibly damaging |
Het |
| Nt5e |
T |
A |
9: 88,252,240 (GRCm39) |
I534N |
probably damaging |
Het |
| Nudt12 |
T |
A |
17: 59,318,071 (GRCm39) |
H58L |
probably damaging |
Het |
| Ocrl |
T |
A |
X: 47,050,993 (GRCm39) |
I74N |
probably damaging |
Het |
| Or1a1 |
A |
G |
11: 74,086,479 (GRCm39) |
H50R |
probably benign |
Het |
| Or2ag13 |
A |
G |
7: 106,473,348 (GRCm39) |
Y35H |
probably damaging |
Het |
| Or5ae2 |
A |
G |
7: 84,505,690 (GRCm39) |
T38A |
probably damaging |
Het |
| Or9k2b |
A |
G |
10: 130,016,701 (GRCm39) |
I16T |
probably benign |
Het |
| Pef1 |
C |
A |
4: 130,014,966 (GRCm39) |
C10* |
probably null |
Het |
| Phf21b |
A |
G |
15: 84,681,547 (GRCm39) |
Y256H |
probably damaging |
Het |
| Phox2a |
G |
T |
7: 101,470,152 (GRCm39) |
|
probably null |
Het |
| Pi16 |
A |
G |
17: 29,546,419 (GRCm39) |
E399G |
possibly damaging |
Het |
| Pipox |
A |
T |
11: 77,772,949 (GRCm39) |
Y321N |
probably damaging |
Het |
| Plin4 |
A |
G |
17: 56,410,522 (GRCm39) |
S1170P |
probably damaging |
Het |
| Pnma2 |
C |
T |
14: 67,154,662 (GRCm39) |
S362L |
possibly damaging |
Het |
| Ppp1r9a |
T |
C |
6: 5,113,710 (GRCm39) |
I738T |
probably damaging |
Het |
| Prkcsh |
A |
T |
9: 21,919,634 (GRCm39) |
K188M |
probably damaging |
Het |
| Rcc1l |
A |
T |
5: 134,192,498 (GRCm39) |
Y309N |
probably damaging |
Het |
| Ripk1 |
A |
G |
13: 34,199,196 (GRCm39) |
T123A |
probably benign |
Het |
| Rps27a |
A |
G |
11: 29,496,299 (GRCm39) |
Y105H |
probably benign |
Het |
| Scaper |
A |
G |
9: 55,724,018 (GRCm39) |
Y641H |
possibly damaging |
Het |
| Scn1a |
C |
A |
2: 66,154,960 (GRCm39) |
Q666H |
probably benign |
Het |
| Scn1a |
T |
A |
2: 66,154,961 (GRCm39) |
Q666L |
probably benign |
Het |
| Sdhaf4 |
T |
C |
1: 24,042,234 (GRCm39) |
|
probably null |
Het |
| Sec14l3 |
A |
G |
11: 4,016,510 (GRCm39) |
|
probably benign |
Het |
| Selp |
C |
A |
1: 163,955,729 (GRCm39) |
|
probably null |
Het |
| Sim1 |
A |
G |
10: 50,785,924 (GRCm39) |
D259G |
probably damaging |
Het |
| Sptan1 |
T |
A |
2: 29,882,013 (GRCm39) |
|
probably benign |
Het |
| Sptbn4 |
T |
A |
7: 27,066,071 (GRCm39) |
E679V |
probably null |
Het |
| Sytl3 |
G |
A |
17: 6,995,726 (GRCm39) |
E169K |
probably benign |
Het |
| Taar1 |
T |
C |
10: 23,797,087 (GRCm39) |
C262R |
probably benign |
Het |
| Taf7l2 |
T |
C |
10: 115,948,570 (GRCm39) |
R319G |
probably benign |
Het |
| Tbc1d32 |
A |
G |
10: 55,893,700 (GRCm39) |
I1291T |
probably benign |
Het |
| Tet1 |
G |
A |
10: 62,649,444 (GRCm39) |
P89S |
probably damaging |
Het |
| Tgfbrap1 |
C |
A |
1: 43,110,795 (GRCm39) |
G7W |
probably damaging |
Het |
| Thada |
A |
G |
17: 84,533,432 (GRCm39) |
V1673A |
possibly damaging |
Het |
| Thbs4 |
A |
G |
13: 92,897,989 (GRCm39) |
V610A |
probably benign |
Het |
| Tmem163 |
A |
G |
1: 127,605,246 (GRCm39) |
S41P |
probably benign |
Het |
| Tmem65 |
A |
T |
15: 58,658,962 (GRCm39) |
H200Q |
probably damaging |
Het |
| Trgc3 |
A |
G |
13: 19,447,535 (GRCm39) |
T163A |
possibly damaging |
Het |
| Trim62 |
T |
C |
4: 128,803,018 (GRCm39) |
V356A |
possibly damaging |
Het |
| Ttn |
T |
C |
2: 76,562,502 (GRCm39) |
D28781G |
probably damaging |
Het |
| Ttn |
C |
T |
2: 76,593,604 (GRCm39) |
V20679I |
probably benign |
Het |
| Tut7 |
A |
T |
13: 59,962,749 (GRCm39) |
Y339* |
probably null |
Het |
| Unc80 |
A |
T |
1: 66,678,407 (GRCm39) |
T2063S |
possibly damaging |
Het |
| Vav3 |
A |
G |
3: 109,413,742 (GRCm39) |
T227A |
probably benign |
Het |
| Vmn2r118 |
A |
T |
17: 55,899,456 (GRCm39) |
I816N |
probably damaging |
Het |
| Vmn2r26 |
T |
A |
6: 124,038,369 (GRCm39) |
M648K |
possibly damaging |
Het |
| Vmn2r88 |
T |
G |
14: 51,650,487 (GRCm39) |
|
probably benign |
Het |
| Vwa3a |
A |
G |
7: 120,389,359 (GRCm39) |
T66A |
probably benign |
Het |
| Xirp2 |
T |
A |
2: 67,341,484 (GRCm39) |
Y1242N |
probably damaging |
Het |
| Ywhag |
G |
A |
5: 135,940,384 (GRCm39) |
T70M |
probably damaging |
Het |
| Zfp248 |
T |
C |
6: 118,405,931 (GRCm39) |
T452A |
probably damaging |
Het |
| Zfp988 |
A |
T |
4: 147,417,344 (GRCm39) |
I593F |
probably damaging |
Het |
|
| Other mutations in Robo4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00090:Robo4
|
APN |
9 |
37,322,400 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00392:Robo4
|
APN |
9 |
37,319,525 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00491:Robo4
|
APN |
9 |
37,317,231 (GRCm39) |
missense |
possibly damaging |
0.52 |
|
IGL00792:Robo4
|
APN |
9 |
37,319,507 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01062:Robo4
|
APN |
9 |
37,317,296 (GRCm39) |
missense |
probably benign |
0.08 |
|
IGL01287:Robo4
|
APN |
9 |
37,324,336 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
IGL02289:Robo4
|
APN |
9 |
37,319,496 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02486:Robo4
|
APN |
9 |
37,319,670 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02851:Robo4
|
APN |
9 |
37,324,678 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL02898:Robo4
|
APN |
9 |
37,319,472 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02965:Robo4
|
APN |
9 |
37,321,765 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
IGL03071:Robo4
|
APN |
9 |
37,315,580 (GRCm39) |
splice site |
probably benign |
|
|
IGL03102:Robo4
|
APN |
9 |
37,315,481 (GRCm39) |
missense |
probably damaging |
1.00 |
|
H8562:Robo4
|
UTSW |
9 |
37,317,106 (GRCm39) |
intron |
probably benign |
|
|
PIT4305001:Robo4
|
UTSW |
9 |
37,322,687 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0056:Robo4
|
UTSW |
9 |
37,315,773 (GRCm39) |
missense |
probably benign |
0.03 |
|
R0068:Robo4
|
UTSW |
9 |
37,315,773 (GRCm39) |
missense |
probably benign |
0.03 |
|
R0233:Robo4
|
UTSW |
9 |
37,313,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0233:Robo4
|
UTSW |
9 |
37,313,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0416:Robo4
|
UTSW |
9 |
37,316,062 (GRCm39) |
splice site |
probably benign |
|
|
R1005:Robo4
|
UTSW |
9 |
37,319,547 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1174:Robo4
|
UTSW |
9 |
37,324,348 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1183:Robo4
|
UTSW |
9 |
37,319,348 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1254:Robo4
|
UTSW |
9 |
37,322,136 (GRCm39) |
critical splice donor site |
probably null |
|
|
R1398:Robo4
|
UTSW |
9 |
37,319,372 (GRCm39) |
critical splice donor site |
probably null |
|
|
R1505:Robo4
|
UTSW |
9 |
37,314,523 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1701:Robo4
|
UTSW |
9 |
37,314,739 (GRCm39) |
missense |
probably benign |
0.44 |
|
R1899:Robo4
|
UTSW |
9 |
37,315,366 (GRCm39) |
splice site |
probably benign |
|
|
R2203:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R2204:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R2351:Robo4
|
UTSW |
9 |
37,322,956 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2448:Robo4
|
UTSW |
9 |
37,313,958 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R2847:Robo4
|
UTSW |
9 |
37,315,772 (GRCm39) |
nonsense |
probably null |
|
|
R2851:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R2852:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R2877:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R3123:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R3124:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R3125:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R3805:Robo4
|
UTSW |
9 |
37,315,734 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R3806:Robo4
|
UTSW |
9 |
37,315,734 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R3892:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R3905:Robo4
|
UTSW |
9 |
37,314,801 (GRCm39) |
nonsense |
probably null |
|
|
R3938:Robo4
|
UTSW |
9 |
37,313,313 (GRCm39) |
start gained |
probably benign |
|
|
R4261:Robo4
|
UTSW |
9 |
37,316,877 (GRCm39) |
missense |
probably benign |
0.04 |
|
R4434:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R4435:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R4561:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R4562:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R4568:Robo4
|
UTSW |
9 |
37,316,118 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R4695:Robo4
|
UTSW |
9 |
37,314,495 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4921:Robo4
|
UTSW |
9 |
37,313,856 (GRCm39) |
missense |
probably benign |
|
|
R5000:Robo4
|
UTSW |
9 |
37,319,664 (GRCm39) |
missense |
probably benign |
0.02 |
|
R5056:Robo4
|
UTSW |
9 |
37,316,102 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5125:Robo4
|
UTSW |
9 |
37,319,256 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5178:Robo4
|
UTSW |
9 |
37,319,256 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5278:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5279:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5285:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5347:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5348:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5361:Robo4
|
UTSW |
9 |
37,324,674 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5403:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5404:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5488:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5489:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5490:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5494:Robo4
|
UTSW |
9 |
37,322,786 (GRCm39) |
frame shift |
probably null |
|
|
R5629:Robo4
|
UTSW |
9 |
37,319,658 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5736:Robo4
|
UTSW |
9 |
37,316,093 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R5796:Robo4
|
UTSW |
9 |
37,322,970 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5987:Robo4
|
UTSW |
9 |
37,322,696 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6178:Robo4
|
UTSW |
9 |
37,316,926 (GRCm39) |
nonsense |
probably null |
|
|
R6189:Robo4
|
UTSW |
9 |
37,314,829 (GRCm39) |
missense |
probably benign |
0.35 |
|
R6365:Robo4
|
UTSW |
9 |
37,322,008 (GRCm39) |
missense |
probably benign |
0.34 |
|
R6528:Robo4
|
UTSW |
9 |
37,315,664 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R6887:Robo4
|
UTSW |
9 |
37,313,363 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R7196:Robo4
|
UTSW |
9 |
37,314,001 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R7408:Robo4
|
UTSW |
9 |
37,322,277 (GRCm39) |
missense |
probably benign |
0.09 |
|
R7419:Robo4
|
UTSW |
9 |
37,314,105 (GRCm39) |
missense |
probably benign |
0.18 |
|
R7486:Robo4
|
UTSW |
9 |
37,316,870 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7707:Robo4
|
UTSW |
9 |
37,324,418 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7839:Robo4
|
UTSW |
9 |
37,322,055 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8079:Robo4
|
UTSW |
9 |
37,313,931 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R8081:Robo4
|
UTSW |
9 |
37,316,936 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8280:Robo4
|
UTSW |
9 |
37,315,372 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8526:Robo4
|
UTSW |
9 |
37,314,801 (GRCm39) |
nonsense |
probably null |
|
|
R8547:Robo4
|
UTSW |
9 |
37,315,674 (GRCm39) |
missense |
possibly damaging |
0.69 |
|
R8735:Robo4
|
UTSW |
9 |
37,319,577 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R8836:Robo4
|
UTSW |
9 |
37,317,130 (GRCm39) |
missense |
unknown |
|
|
R8889:Robo4
|
UTSW |
9 |
37,314,601 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9018:Robo4
|
UTSW |
9 |
37,315,520 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9182:Robo4
|
UTSW |
9 |
37,313,206 (GRCm39) |
start gained |
probably benign |
|
|
R9375:Robo4
|
UTSW |
9 |
37,316,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9621:Robo4
|
UTSW |
9 |
37,317,509 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTAGGTCCCAGGAGCCATTG -3'
(R):5'- TTAATGTTAGCCAGTCAGCCAGATAC -3'
Sequencing Primer
(F):5'- CAGGAGCCATTGGAGCTG -3'
(R):5'- GTTAGCCAGTCAGCCAGATACTTACC -3'
|
| Posted On |
2014-06-23 |
Incidental Mutation