Mutagenetix > Incidental Mutation

Incidental Mutation 'R1835:Cfap54'

205187

Institutional Source

Beutler Lab

Gene Symbol

Cfap54

Ensembl Gene

ENSMUSG00000020014

Gene Name

cilia and flagella associated protein 54

Synonyms

LOC380653, Gm872, 4930485B16Rik

MMRRC Submission

039862-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R1835 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

92775619-93081618 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 92962375 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 1674 (D1674V)

Ref Sequence

ENSEMBL: ENSMUSP00000148636 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168110] [ENSMUST00000212902]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000168110
AA Change: D1674V

PolyPhen 2 Score 0.354 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000129517
Gene: ENSMUSG00000020014
AA Change: D1674V

Domain	Start	End	E-Value	Type
low complexity region	3	37	N/A	INTRINSIC
low complexity region	39	48	N/A	INTRINSIC
Pfam:DUF4486	104	642	1.1e-269	PFAM
low complexity region	842	851	N/A	INTRINSIC
low complexity region	902	915	N/A	INTRINSIC
Blast:FN3	916	1002	4e-48	BLAST
low complexity region	1409	1426	N/A	INTRINSIC
low complexity region	1974	1984	N/A	INTRINSIC
low complexity region	2354	2370	N/A	INTRINSIC
low complexity region	2500	2513	N/A	INTRINSIC
low complexity region	2605	2616	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000212902
AA Change: D1674V

PolyPhen 2 Score 0.354 (Sensitivity: 0.90; Specificity: 0.89)

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 94.9%
20x: 91.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous inactivation of this gene causes background-dependent lethality and hydroencephaly, male sterility associated with defects in spermiogenesis, and impaired mucociliary clearance. Airway epithelial cilia show structural defects and a decrease in ciliary beat frequency and cilia-driven flow. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 97 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610507B11Rik	G	T	11: 78,287,750 (GRCm38)	V1993F	probably damaging	Het
4933425L06Rik	C	A	13: 105,082,194 (GRCm38)	A12E	unknown	Het
Acss2	T	A	2: 155,558,630 (GRCm38)	Y530N	probably damaging	Het
Adam4	T	C	12: 81,419,559 (GRCm38)	I763V	probably benign	Het
Aipl1	T	G	11: 72,030,499 (GRCm38)	K190T	possibly damaging	Het
Alms1	T	A	6: 85,678,503 (GRCm38)	S3344T	possibly damaging	Het
Alpi	A	G	1: 87,099,414 (GRCm38)	V381A	possibly damaging	Het
Ankfn1	A	T	11: 89,447,618 (GRCm38)	S365R	probably benign	Het
Aox2	G	A	1: 58,308,991 (GRCm38)	A623T	probably benign	Het
Apc	T	A	18: 34,317,077 (GRCm38)	L2308Q	probably damaging	Het
Atp9b	C	T	18: 80,778,883 (GRCm38)	V501I	probably benign	Het
Baz2b	T	C	2: 59,901,819 (GRCm38)	E1994G	probably benign	Het
Cacna1a	T	C	8: 84,581,357 (GRCm38)		probably null	Het
Cacna1h	C	A	17: 25,392,076 (GRCm38)	V583L	probably benign	Het
Cd55	T	C	1: 130,447,609 (GRCm38)		probably benign	Het
Cep192	T	A	18: 67,804,424 (GRCm38)	S75T	possibly damaging	Het
Churc1	T	C	12: 76,773,297 (GRCm38)	F27L	possibly damaging	Het
Coro1c	A	G	5: 113,848,543 (GRCm38)	I280T	probably benign	Het
Creb1	C	T	1: 64,550,950 (GRCm38)	Q32*	probably null	Het
Cyp2b9	A	G	7: 26,200,783 (GRCm38)	T339A	probably benign	Het
Dctn3	T	C	4: 41,720,813 (GRCm38)	R51G	probably damaging	Het
Ddb1	T	C	19: 10,626,593 (GRCm38)	V888A	probably damaging	Het
Disp1	A	G	1: 183,089,000 (GRCm38)	Y619H	probably damaging	Het
Dnajc9	T	C	14: 20,388,334 (GRCm38)	D96G	possibly damaging	Het
Eepd1	A	G	9: 25,482,868 (GRCm38)	T143A	possibly damaging	Het
Eps8	T	A	6: 137,522,279 (GRCm38)	K204*	probably null	Het
Ercc5	T	A	1: 44,180,875 (GRCm38)	S1102R	probably benign	Het
Ergic2	T	A	6: 148,189,581 (GRCm38)	Y211F	possibly damaging	Het
Fam135b	G	T	15: 71,490,711 (GRCm38)	L274M	probably damaging	Het
Fat3	G	A	9: 15,998,088 (GRCm38)	T2206I	probably damaging	Het
Fat4	A	G	3: 38,983,571 (GRCm38)	I3791V	probably benign	Het
Gemin4	A	G	11: 76,213,296 (GRCm38)	M213T	possibly damaging	Het
Gnai3	T	C	3: 108,118,407 (GRCm38)	M119V	probably benign	Het
Heatr5b	A	T	17: 78,773,563 (GRCm38)	L1420Q	probably damaging	Het
Herc2	G	T	7: 56,206,765 (GRCm38)	G3918*	probably null	Het
Ints9	A	G	14: 65,032,256 (GRCm38)	Y465C	probably damaging	Het
Ist1	A	G	8: 109,678,883 (GRCm38)	V175A	probably damaging	Het
Kcnj12	T	C	11: 61,069,557 (GRCm38)	L227P	possibly damaging	Het
Kcnq5	T	C	1: 21,466,387 (GRCm38)	S416G	probably benign	Het
Kdm3a	T	A	6: 71,613,956 (GRCm38)	T295S	probably benign	Het
Kif1c	T	A	11: 70,708,971 (GRCm38)	M479K	probably damaging	Het
Kif20b	T	C	19: 34,956,038 (GRCm38)	L83P	probably damaging	Het
Kndc1	A	G	7: 139,927,711 (GRCm38)	E1194G	probably damaging	Het
Llgl1	A	G	11: 60,704,730 (GRCm38)	M81V	probably benign	Het
Map4k5	C	A	12: 69,824,662 (GRCm38)	M495I	probably damaging	Het
Mest	C	T	6: 30,742,791 (GRCm38)	R146C	probably benign	Het
Mettl24	T	A	10: 40,737,816 (GRCm38)		probably null	Het
Mical1	T	C	10: 41,483,535 (GRCm38)	S586P	probably benign	Het
Mrgpra3	G	T	7: 47,589,946 (GRCm38)	Y77*	probably null	Het
Mss51	A	T	14: 20,483,178 (GRCm38)	C408*	probably null	Het
Myh6	T	C	14: 54,957,401 (GRCm38)	T666A	probably benign	Het
Myo10	T	C	15: 25,805,587 (GRCm38)	C1685R	possibly damaging	Het
Naip5	A	T	13: 100,223,218 (GRCm38)	Y503*	probably null	Het
Neil1	A	C	9: 57,146,604 (GRCm38)	F144C	probably damaging	Het
Nipbl	T	A	15: 8,343,517 (GRCm38)	I1082F	possibly damaging	Het
Nkiras1	T	C	14: 18,276,732 (GRCm38)	V7A	probably damaging	Het
Ntng2	G	A	2: 29,197,057 (GRCm38)	Q384*	probably null	Het
Ocrl	T	A	X: 47,962,116 (GRCm38)	I74N	probably damaging	Het
Olfr137	A	T	17: 38,305,312 (GRCm38)	S50T	probably benign	Het
Olfr1385	A	T	11: 49,494,670 (GRCm38)	I46F	probably damaging	Het
Olfr1408	A	G	1: 173,130,815 (GRCm38)	V134A	probably benign	Het
Olfr381	A	C	11: 73,486,374 (GRCm38)	V150G	probably benign	Het
Olfr535	A	G	7: 140,492,709 (GRCm38)	S24G	probably benign	Het
Olfr761	C	T	17: 37,952,385 (GRCm38)	G213E	possibly damaging	Het
Patj	A	G	4: 98,491,590 (GRCm38)	D151G	probably benign	Het
Plpbp	T	C	8: 27,049,231 (GRCm38)	V126A	probably damaging	Het
Pold2	A	G	11: 5,873,454 (GRCm38)	L325P	possibly damaging	Het
Ppp1r12c	A	C	7: 4,483,651 (GRCm38)	S480A	probably damaging	Het
Pum1	T	C	4: 130,701,048 (GRCm38)	S124P	possibly damaging	Het
Pwp2	C	G	10: 78,179,091 (GRCm38)	G353A	probably damaging	Het
Reln	C	A	5: 21,979,002 (GRCm38)	Q1666H	probably damaging	Het
Rnf19a	T	C	15: 36,265,925 (GRCm38)	I9V	probably benign	Het
Ryr2	T	C	13: 11,769,878 (GRCm38)	H1063R	probably benign	Het
Samd14	C	G	11: 95,023,600 (GRCm38)	D361E	probably damaging	Het
Samd15	A	T	12: 87,201,843 (GRCm38)	N365I	probably damaging	Het
Sec16b	A	G	1: 157,531,312 (GRCm38)	H105R	probably benign	Het
Sez6	T	C	11: 77,953,503 (GRCm38)	S51P	probably benign	Het
Sh3bp1	T	A	15: 78,905,150 (GRCm38)	L236Q	probably damaging	Het
Sspo	C	T	6: 48,457,340 (GRCm38)	T991I	probably damaging	Het
Suco	A	T	1: 161,859,500 (GRCm38)	L97*	probably null	Het
Tab1	C	A	15: 80,148,296 (GRCm38)	R35S	probably benign	Het
Tet3	C	A	6: 83,404,163 (GRCm38)	S341I	possibly damaging	Het
Tlr1	A	T	5: 64,925,700 (GRCm38)	D511E	probably benign	Het
Tmem132b	A	C	5: 125,785,899 (GRCm38)	D656A	probably damaging	Het
Tmtc4	T	A	14: 122,941,988 (GRCm38)		probably null	Het
Trmo	T	C	4: 46,380,158 (GRCm38)	T404A	probably damaging	Het
Trpm1	A	G	7: 64,230,268 (GRCm38)	K790E	probably damaging	Het
Ulk4	C	A	9: 121,168,184 (GRCm38)	R774M	probably null	Het
Ush2a	C	T	1: 188,451,818 (GRCm38)	L1440F	probably benign	Het
Usp16	A	G	16: 87,480,907 (GRCm38)	K682E	probably damaging	Het
Virma	T	C	4: 11,540,511 (GRCm38)	S1471P	probably benign	Het
Vmn1r177	A	T	7: 23,865,686 (GRCm38)	I255N	probably damaging	Het
Vmn2r61	A	T	7: 42,266,652 (GRCm38)	R230*	probably null	Het
Vps13c	T	A	9: 67,993,013 (GRCm38)	F3671L	probably benign	Het
Washc5	T	C	15: 59,359,340 (GRCm38)	N358S	possibly damaging	Het
Wdr72	A	G	9: 74,151,617 (GRCm38)	K331E	probably damaging	Het
Zfp986	A	T	4: 145,899,235 (GRCm38)	K155I	probably benign	Het

Other mutations in Cfap54

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01329:Cfap54	APN	10	93,081,523 (GRCm38)	missense	unknown
IGL02034:Cfap54	APN	10	93,061,485 (GRCm38)	missense	probably damaging	0.99
IGL02082:Cfap54	APN	10	93,081,458 (GRCm38)	missense	unknown
IGL02434:Cfap54	APN	10	93,066,754 (GRCm38)	missense	probably benign	0.20
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0040:Cfap54	UTSW	10	92,977,039 (GRCm38)	missense	probably benign	0.33
R0044:Cfap54	UTSW	10	93,035,433 (GRCm38)	missense	probably null	0.46
R0086:Cfap54	UTSW	10	93,028,594 (GRCm38)	missense	possibly damaging	0.86
R0104:Cfap54	UTSW	10	93,028,652 (GRCm38)	missense	probably damaging	1.00
R0194:Cfap54	UTSW	10	93,034,662 (GRCm38)	unclassified	probably benign
R0234:Cfap54	UTSW	10	92,899,160 (GRCm38)	nonsense	probably null
R0308:Cfap54	UTSW	10	92,885,364 (GRCm38)	missense	unknown
R0332:Cfap54	UTSW	10	93,035,457 (GRCm38)	missense	probably damaging	1.00
R0409:Cfap54	UTSW	10	92,776,213 (GRCm38)	missense	probably benign	0.00
R0433:Cfap54	UTSW	10	92,979,080 (GRCm38)	splice site	probably benign
R0436:Cfap54	UTSW	10	93,038,975 (GRCm38)	missense	possibly damaging	0.95
R0463:Cfap54	UTSW	10	92,874,943 (GRCm38)	critical splice donor site	probably null
R0523:Cfap54	UTSW	10	92,908,883 (GRCm38)	utr 3 prime	probably benign
R0551:Cfap54	UTSW	10	93,025,122 (GRCm38)	missense	probably benign	0.35
R0595:Cfap54	UTSW	10	92,884,736 (GRCm38)	missense	unknown
R0617:Cfap54	UTSW	10	92,829,650 (GRCm38)	splice site	probably benign
R0632:Cfap54	UTSW	10	92,885,096 (GRCm38)	missense	unknown
R0730:Cfap54	UTSW	10	93,034,737 (GRCm38)	missense	probably benign	0.05
R0786:Cfap54	UTSW	10	92,967,535 (GRCm38)	missense	possibly damaging	0.72
R0883:Cfap54	UTSW	10	92,870,669 (GRCm38)	missense	unknown
R1004:Cfap54	UTSW	10	93,066,696 (GRCm38)	splice site	probably benign
R1033:Cfap54	UTSW	10	92,839,449 (GRCm38)	missense	probably benign	0.07
R1168:Cfap54	UTSW	10	92,937,920 (GRCm38)	missense	probably damaging	0.99
R1186:Cfap54	UTSW	10	92,875,994 (GRCm38)	missense	unknown
R1429:Cfap54	UTSW	10	92,821,038 (GRCm38)	missense	probably benign	0.01
R1443:Cfap54	UTSW	10	92,932,721 (GRCm38)	missense	probably damaging	1.00
R1467:Cfap54	UTSW	10	92,969,763 (GRCm38)	missense	probably benign	0.01
R1557:Cfap54	UTSW	10	92,984,227 (GRCm38)	missense	possibly damaging	0.68
R1687:Cfap54	UTSW	10	92,932,640 (GRCm38)	missense	probably damaging	1.00
R1690:Cfap54	UTSW	10	93,035,442 (GRCm38)	missense	possibly damaging	0.95
R1711:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R1756:Cfap54	UTSW	10	93,048,061 (GRCm38)	missense	probably damaging	1.00
R1769:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably null
R1889:Cfap54	UTSW	10	93,034,710 (GRCm38)	missense	possibly damaging	0.94
R1915:Cfap54	UTSW	10	92,884,702 (GRCm38)	missense	unknown
R1958:Cfap54	UTSW	10	92,997,342 (GRCm38)	missense	probably benign	0.18
R2005:Cfap54	UTSW	10	92,884,768 (GRCm38)	missense	unknown
R2018:Cfap54	UTSW	10	93,016,604 (GRCm38)	missense	probably benign	0.00
R2045:Cfap54	UTSW	10	93,038,809 (GRCm38)	splice site	probably null
R2059:Cfap54	UTSW	10	92,942,979 (GRCm38)	unclassified	probably benign
R2100:Cfap54	UTSW	10	93,001,937 (GRCm38)	missense	possibly damaging	0.84
R2110:Cfap54	UTSW	10	92,886,367 (GRCm38)	missense	unknown
R2392:Cfap54	UTSW	10	93,025,011 (GRCm38)	critical splice donor site	probably null
R2508:Cfap54	UTSW	10	92,997,374 (GRCm38)	missense	possibly damaging	0.72
R2852:Cfap54	UTSW	10	92,940,155 (GRCm38)	missense	probably damaging	1.00
R2857:Cfap54	UTSW	10	93,045,282 (GRCm38)	missense	probably damaging	0.99
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R3107:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3108:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3157:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3158:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3159:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3161:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3508:Cfap54	UTSW	10	92,885,424 (GRCm38)	missense	unknown
R3730:Cfap54	UTSW	10	93,011,473 (GRCm38)	nonsense	probably null
R3770:Cfap54	UTSW	10	92,878,536 (GRCm38)	missense	unknown
R3776:Cfap54	UTSW	10	93,045,100 (GRCm38)	missense	probably damaging	1.00
R3778:Cfap54	UTSW	10	92,904,344 (GRCm38)	utr 3 prime	probably benign
R3795:Cfap54	UTSW	10	92,942,873 (GRCm38)	unclassified	probably benign
R3834:Cfap54	UTSW	10	92,801,123 (GRCm38)	splice site	probably benign
R3891:Cfap54	UTSW	10	93,038,846 (GRCm38)	missense	possibly damaging	0.87
R3932:Cfap54	UTSW	10	92,829,757 (GRCm38)	missense	probably benign	0.03
R3973:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3974:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3976:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3978:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R4190:Cfap54	UTSW	10	92,885,023 (GRCm38)	missense	unknown
R4389:Cfap54	UTSW	10	92,967,500 (GRCm38)	missense	probably benign	0.37
R4542:Cfap54	UTSW	10	93,025,129 (GRCm38)	missense	probably benign	0.12
R4564:Cfap54	UTSW	10	92,839,540 (GRCm38)	unclassified	probably benign
R4576:Cfap54	UTSW	10	93,043,228 (GRCm38)	critical splice donor site	probably null
R4620:Cfap54	UTSW	10	92,969,757 (GRCm38)	missense	probably benign	0.01
R4714:Cfap54	UTSW	10	92,815,918 (GRCm38)	missense	probably benign	0.01
R4762:Cfap54	UTSW	10	93,061,453 (GRCm38)	splice site	probably null
R4776:Cfap54	UTSW	10	92,972,694 (GRCm38)	missense	possibly damaging	0.96
R4819:Cfap54	UTSW	10	92,836,477 (GRCm38)	nonsense	probably null
R4827:Cfap54	UTSW	10	92,902,075 (GRCm38)	utr 3 prime	probably benign
R4832:Cfap54	UTSW	10	92,967,528 (GRCm38)	missense	probably benign	0.01
R4965:Cfap54	UTSW	10	93,066,799 (GRCm38)	missense	probably benign	0.23
R5001:Cfap54	UTSW	10	92,964,534 (GRCm38)	missense	probably benign	0.01
R5060:Cfap54	UTSW	10	93,039,151 (GRCm38)	missense	probably damaging	1.00
R5067:Cfap54	UTSW	10	93,066,766 (GRCm38)	missense	probably benign	0.17
R5069:Cfap54	UTSW	10	92,937,774 (GRCm38)	missense	probably benign
R5094:Cfap54	UTSW	10	92,898,999 (GRCm38)	utr 3 prime	probably benign
R5109:Cfap54	UTSW	10	92,937,891 (GRCm38)	missense	probably benign	0.03
R5127:Cfap54	UTSW	10	92,886,387 (GRCm38)	splice site	probably null
R5143:Cfap54	UTSW	10	93,029,158 (GRCm38)	missense	possibly damaging	0.73
R5147:Cfap54	UTSW	10	92,937,838 (GRCm38)	missense	probably benign	0.00
R5158:Cfap54	UTSW	10	93,065,197 (GRCm38)	missense	probably damaging	1.00
R5256:Cfap54	UTSW	10	93,045,023 (GRCm38)	splice site	probably null
R5256:Cfap54	UTSW	10	92,935,091 (GRCm38)	nonsense	probably null
R5266:Cfap54	UTSW	10	92,815,902 (GRCm38)	missense	probably benign	0.16
R5304:Cfap54	UTSW	10	92,821,106 (GRCm38)	missense	probably damaging	0.97
R5369:Cfap54	UTSW	10	93,061,257 (GRCm38)	intron	probably benign
R5406:Cfap54	UTSW	10	93,001,858 (GRCm38)	missense	probably benign	0.33
R5471:Cfap54	UTSW	10	93,028,660 (GRCm38)	missense	probably damaging	1.00
R5485:Cfap54	UTSW	10	93,029,117 (GRCm38)	missense	probably damaging	1.00
R5540:Cfap54	UTSW	10	92,972,608 (GRCm38)	missense	possibly damaging	0.85
R5586:Cfap54	UTSW	10	92,972,611 (GRCm38)	nonsense	probably null
R5614:Cfap54	UTSW	10	93,045,049 (GRCm38)	missense	probably damaging	1.00
R5634:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably benign
R5680:Cfap54	UTSW	10	92,979,017 (GRCm38)	nonsense	probably null
R5797:Cfap54	UTSW	10	92,967,576 (GRCm38)	missense	probably benign	0.11
R5859:Cfap54	UTSW	10	93,016,524 (GRCm38)	nonsense	probably null
R5878:Cfap54	UTSW	10	92,964,561 (GRCm38)	missense	probably benign	0.01
R5910:Cfap54	UTSW	10	93,065,181 (GRCm38)	missense	probably damaging	0.99
R5936:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R5994:Cfap54	UTSW	10	93,039,081 (GRCm38)	missense	probably damaging	0.99
R6080:Cfap54	UTSW	10	93,045,335 (GRCm38)	missense	possibly damaging	0.64
R6268:Cfap54	UTSW	10	93,038,909 (GRCm38)	missense	probably damaging	1.00
R6296:Cfap54	UTSW	10	93,066,846 (GRCm38)	missense	probably damaging	1.00
R6409:Cfap54	UTSW	10	92,967,492 (GRCm38)	missense	probably benign	0.04
R6545:Cfap54	UTSW	10	92,836,457 (GRCm38)	missense	probably benign	0.31
R6570:Cfap54	UTSW	10	92,815,958 (GRCm38)	missense	unknown
R6597:Cfap54	UTSW	10	92,999,040 (GRCm38)	missense	possibly damaging	0.85
R6702:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6703:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6720:Cfap54	UTSW	10	92,821,119 (GRCm38)	missense	probably benign	0.07
R6841:Cfap54	UTSW	10	92,875,015 (GRCm38)	missense	unknown
R6910:Cfap54	UTSW	10	92,836,512 (GRCm38)	missense	probably benign	0.29
R6953:Cfap54	UTSW	10	92,994,678 (GRCm38)	missense	probably benign	0.19
R7009:Cfap54	UTSW	10	92,875,019 (GRCm38)	missense	unknown
R7129:Cfap54	UTSW	10	93,016,571 (GRCm38)	missense	probably benign	0.06
R7131:Cfap54	UTSW	10	92,821,104 (GRCm38)	missense	probably benign	0.03
R7171:Cfap54	UTSW	10	92,776,210 (GRCm38)	missense	probably damaging	0.99
R7189:Cfap54	UTSW	10	92,937,728 (GRCm38)	missense	unknown
R7225:Cfap54	UTSW	10	92,904,374 (GRCm38)	missense	unknown
R7270:Cfap54	UTSW	10	92,839,458 (GRCm38)	missense	probably benign	0.03
R7323:Cfap54	UTSW	10	92,801,138 (GRCm38)	missense	probably benign	0.00
R7380:Cfap54	UTSW	10	93,047,978 (GRCm38)	missense	probably damaging	1.00
R7395:Cfap54	UTSW	10	92,884,703 (GRCm38)	missense	unknown
R7411:Cfap54	UTSW	10	92,868,755 (GRCm38)	missense	unknown
R7503:Cfap54	UTSW	10	92,887,436 (GRCm38)	splice site	probably null
R7622:Cfap54	UTSW	10	92,956,944 (GRCm38)	missense	unknown
R7679:Cfap54	UTSW	10	92,967,512 (GRCm38)	missense	probably benign	0.01
R7776:Cfap54	UTSW	10	92,868,741 (GRCm38)	missense	unknown
R7844:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R7980:Cfap54	UTSW	10	92,982,060 (GRCm38)	missense	possibly damaging	0.95
R7988:Cfap54	UTSW	10	92,902,079 (GRCm38)	missense	unknown
R8101:Cfap54	UTSW	10	92,884,796 (GRCm38)	missense	unknown
R8119:Cfap54	UTSW	10	92,868,810 (GRCm38)	missense	unknown
R8134:Cfap54	UTSW	10	92,878,516 (GRCm38)	missense	unknown
R8168:Cfap54	UTSW	10	92,908,877 (GRCm38)	missense	unknown
R8179:Cfap54	UTSW	10	92,997,316 (GRCm38)	missense	possibly damaging	0.68
R8392:Cfap54	UTSW	10	92,962,417 (GRCm38)	missense	unknown
R8436:Cfap54	UTSW	10	92,964,536 (GRCm38)	missense	unknown
R8505:Cfap54	UTSW	10	92,978,993 (GRCm38)	missense	probably benign	0.03
R8671:Cfap54	UTSW	10	92,955,072 (GRCm38)	missense	unknown
R8716:Cfap54	UTSW	10	92,964,632 (GRCm38)	missense	probably benign	0.00
R8816:Cfap54	UTSW	10	92,878,592 (GRCm38)	missense	unknown
R8822:Cfap54	UTSW	10	93,039,141 (GRCm38)	missense	probably benign	0.09
R8827:Cfap54	UTSW	10	92,938,248 (GRCm38)	missense	unknown
R8920:Cfap54	UTSW	10	92,940,337 (GRCm38)	critical splice acceptor site	probably null
R8924:Cfap54	UTSW	10	93,001,823 (GRCm38)	missense	probably damaging	0.99
R8954:Cfap54	UTSW	10	93,043,393 (GRCm38)	missense	probably damaging	1.00
R8963:Cfap54	UTSW	10	93,028,700 (GRCm38)	nonsense	probably null
R9010:Cfap54	UTSW	10	92,899,059 (GRCm38)	missense	unknown
R9017:Cfap54	UTSW	10	92,816,021 (GRCm38)	missense	probably benign	0.07
R9093:Cfap54	UTSW	10	92,815,908 (GRCm38)	missense	probably benign	0.03
R9095:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R9142:Cfap54	UTSW	10	92,984,235 (GRCm38)	missense	possibly damaging	0.87
R9178:Cfap54	UTSW	10	92,994,717 (GRCm38)	missense	probably benign	0.10
R9196:Cfap54	UTSW	10	93,037,891 (GRCm38)	missense	probably benign	0.22
R9203:Cfap54	UTSW	10	93,045,128 (GRCm38)	missense	probably benign	0.30
R9258:Cfap54	UTSW	10	92,935,098 (GRCm38)	missense	unknown
R9275:Cfap54	UTSW	10	93,039,186 (GRCm38)	missense	possibly damaging	0.86
R9287:Cfap54	UTSW	10	92,969,703 (GRCm38)	missense	possibly damaging	0.50
R9289:Cfap54	UTSW	10	92,821,074 (GRCm38)	missense	possibly damaging	0.83
R9310:Cfap54	UTSW	10	92,962,315 (GRCm38)	missense	unknown
R9397:Cfap54	UTSW	10	92,997,285 (GRCm38)	missense	probably damaging	0.96
R9462:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R9697:Cfap54	UTSW	10	92,956,989 (GRCm38)	missense	unknown
R9746:Cfap54	UTSW	10	92,801,219 (GRCm38)	missense	probably benign	0.03
R9755:Cfap54	UTSW	10	92,921,368 (GRCm38)	missense	unknown
X0022:Cfap54	UTSW	10	92,932,614 (GRCm38)	missense	probably damaging	1.00
X0022:Cfap54	UTSW	10	92,878,603 (GRCm38)	missense	unknown
X0027:Cfap54	UTSW	10	93,001,888 (GRCm38)	missense	possibly damaging	0.86
X0027:Cfap54	UTSW	10	92,878,538 (GRCm38)	missense	unknown
Z1177:Cfap54	UTSW	10	92,979,026 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAATGTCTACTTATCCCAAGTTTCACG -3'
(R):5'- CGCAGTGTTACACCTGTGAATAG -3'

Sequencing Primer
(F):5'- TATCCCAAGTTTCACGATAAAAGGC -3'
(R):5'- CCTGTGAATAGTACATAACAAGTGG -3'

Posted On

2014-06-23