Mutagenetix > Incidental Mutation

Incidental Mutation 'R1829:Atp2b2'

207100

Institutional Source

Beutler Lab

Gene Symbol

Atp2b2

Ensembl Gene

ENSMUSG00000030302

Gene Name

ATPase, Ca++ transporting, plasma membrane 2

Synonyms

PMCA2, Gena300, wms, D6Abb2e, jog, Tmy

MMRRC Submission

039856-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.889) question?

Stock #

R1829 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

113720803-114019574 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 113750329 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 677 (R677S)

Ref Sequence

ENSEMBL: ENSMUSP00000098605 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089003] [ENSMUST00000101044] [ENSMUST00000101045] [ENSMUST00000152831] [ENSMUST00000205052]

AlphaFold

Q9R0K7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089003
AA Change: R632S

PolyPhen 2 Score 0.922 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000086398
Gene: ENSMUSG00000030302
AA Change: R632S

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	1.7e-56	PFAM
Pfam:Hydrolase	448	787	3.9e-25	PFAM
Pfam:HAD	451	784	2.4e-16	PFAM
Pfam:Hydrolase_like2	497	593	9.4e-17	PFAM
Pfam:Hydrolase_3	745	820	1.7e-6	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	7.3e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1144	1.4e-31	PFAM
low complexity region	1151	1166	N/A	INTRINSIC
low complexity region	1175	1189	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101044
AA Change: R677S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098605
Gene: ENSMUSG00000030302
AA Change: R677S

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	155	307	4.2e-28	PFAM
low complexity region	313	330	N/A	INTRINSIC
low complexity region	337	356	N/A	INTRINSIC
Pfam:E1-E2_ATPase	373	488	1.4e-13	PFAM
Pfam:Hydrolase	493	832	8.1e-16	PFAM
Pfam:HAD	496	829	6.3e-21	PFAM
Pfam:Cation_ATPase	542	638	4.4e-17	PFAM
Pfam:Hydrolase_3	791	865	8.3e-7	PFAM
transmembrane domain	878	900	N/A	INTRINSIC
Pfam:Cation_ATPase_C	902	1084	2.5e-47	PFAM
low complexity region	1102	1115	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1126	1178	2.4e-30	PFAM
low complexity region	1196	1211	N/A	INTRINSIC
low complexity region	1220	1234	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000101045
AA Change: R632S

PolyPhen 2 Score 0.922 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000098606
Gene: ENSMUSG00000030302
AA Change: R632S

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	1.7e-56	PFAM
Pfam:Hydrolase	448	787	3.9e-25	PFAM
Pfam:HAD	451	784	2.4e-16	PFAM
Pfam:Hydrolase_like2	497	593	9.4e-17	PFAM
Pfam:Hydrolase_3	745	820	1.7e-6	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	7.3e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1144	1.4e-31	PFAM
low complexity region	1151	1166	N/A	INTRINSIC
low complexity region	1175	1189	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000152831
AA Change: R632S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000138165
Gene: ENSMUSG00000030302
AA Change: R632S

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	156	444	6.1e-57	PFAM
Pfam:Hydrolase	448	787	1.4e-25	PFAM
Pfam:HAD	451	784	7.7e-17	PFAM
Pfam:Hydrolase_like2	497	593	4.4e-17	PFAM
Pfam:Hydrolase_3	745	820	4.2e-7	PFAM
transmembrane domain	833	855	N/A	INTRINSIC
Pfam:Cation_ATPase_C	857	1039	2.7e-46	PFAM
low complexity region	1057	1070	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1081	1149	1.3e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000205052
AA Change: R632S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000145174
Gene: ENSMUSG00000030302
AA Change: R632S

Domain	Start	End	E-Value	Type
Cation_ATPase_N	47	123	1.21e-4	SMART
Pfam:E1-E2_ATPase	155	310	1.9e-28	PFAM
Pfam:E1-E2_ATPase	328	443	1.1e-13	PFAM
Pfam:HAD	451	780	2.7e-19	PFAM
Pfam:Cation_ATPase	497	593	5.8e-17	PFAM
Pfam:Hydrolase	576	783	2e-8	PFAM
Pfam:Hydrolase_3	711	816	2.3e-7	PFAM
transmembrane domain	829	851	N/A	INTRINSIC
Pfam:Cation_ATPase_C	853	1035	2.5e-47	PFAM
low complexity region	1053	1066	N/A	INTRINSIC
Pfam:ATP_Ca_trans_C	1077	1129	2.6e-30	PFAM
low complexity region	1147	1162	N/A	INTRINSIC
low complexity region	1171	1185	N/A	INTRINSIC

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants exhibit slower growth, balance problems, and deafness, associated with cerebellar abnormalities, an absence of otoconia, and abnormalities of the organ of Corti. Heterozygotes exhibit appreciable age-dependent hearing loss. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 114 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars1	A	T	8: 111,769,338 (GRCm39)	D287V	probably damaging	Het
Abca12	A	G	1: 71,334,188 (GRCm39)	C1105R	probably benign	Het
Abca8b	T	A	11: 109,833,167 (GRCm39)	N1178Y	probably damaging	Het
Abhd12	A	G	2: 150,685,318 (GRCm39)	L189P	probably damaging	Het
Acap2	G	T	16: 30,929,752 (GRCm39)	N435K	probably damaging	Het
Adam6b	G	T	12: 113,453,545 (GRCm39)	G121C	probably damaging	Het
Adgrb1	C	A	15: 74,452,435 (GRCm39)	C200*	probably null	Het
Agbl5	T	C	5: 31,060,408 (GRCm39)	S730P	possibly damaging	Het
Ahsg	G	A	16: 22,711,078 (GRCm39)		probably benign	Het
Aldh9a1	A	T	1: 167,189,423 (GRCm39)	K390N	probably benign	Het
Alpk2	T	A	18: 65,427,165 (GRCm39)	H1857L	possibly damaging	Het
Apip	T	A	2: 102,919,007 (GRCm39)	N102K	probably benign	Het
Asxl2	T	C	12: 3,507,125 (GRCm39)	S106P	probably damaging	Het
Barhl1	A	C	2: 28,799,857 (GRCm39)	M256R	probably damaging	Het
Cacnb2	A	T	2: 14,990,775 (GRCm39)	Q619L	possibly damaging	Het
Ccdc137	C	T	11: 120,349,038 (GRCm39)	P23L	probably benign	Het
Cdh11	A	T	8: 103,361,273 (GRCm39)	N688K	possibly damaging	Het
Cdh18	C	T	15: 23,173,938 (GRCm39)	P51S	probably damaging	Het
Cdin1	G	T	2: 115,473,173 (GRCm39)	R101L	possibly damaging	Het
Cfhr1	A	T	1: 139,481,338 (GRCm39)	Y181N	probably damaging	Het
Chmp3	A	G	6: 71,537,923 (GRCm39)	D50G	probably benign	Het
Crem	T	C	18: 3,295,037 (GRCm39)		probably null	Het
Cyb561a3	G	A	19: 10,559,757 (GRCm39)	W27*	probably null	Het
Cyp2d12	C	A	15: 82,442,257 (GRCm39)	N297K	possibly damaging	Het
Dclk2	C	T	3: 86,712,946 (GRCm39)	R503Q	possibly damaging	Het
Dnah12	T	A	14: 26,522,032 (GRCm39)	N1948K	probably damaging	Het
Dnah12	T	C	14: 26,494,980 (GRCm39)	L1346P	probably damaging	Het
Dsp	T	G	13: 38,377,171 (GRCm39)	L1652R	probably damaging	Het
Dstyk	G	A	1: 132,377,333 (GRCm39)	S66N	probably benign	Het
Ehhadh	T	C	16: 21,580,928 (GRCm39)	E688G	probably damaging	Het
Emsy	G	T	7: 98,251,937 (GRCm39)	H688N	possibly damaging	Het
Emsy	T	A	7: 98,251,936 (GRCm39)	H688L	possibly damaging	Het
Endod1	A	G	9: 14,268,222 (GRCm39)	L421P	probably damaging	Het
Fam222b	C	T	11: 78,045,861 (GRCm39)	P346L	probably damaging	Het
Fam3c	G	A	6: 22,309,436 (GRCm39)	R182W	probably damaging	Het
Gck	T	C	11: 5,860,984 (GRCm39)	D29G	probably damaging	Het
Gm10320	C	A	13: 98,626,207 (GRCm39)	R59L	probably damaging	Het
Gm21798	C	T	15: 64,689,675 (GRCm39)		probably benign	Het
Gm9376	A	G	14: 118,504,957 (GRCm39)	T130A	possibly damaging	Het
Gpr153	T	A	4: 152,366,849 (GRCm39)	I334N	possibly damaging	Het
Greb1l	T	C	18: 10,509,314 (GRCm39)	L542P	probably damaging	Het
H2-T15	G	T	17: 36,368,896 (GRCm39)	F61L	probably damaging	Het
Hacl1	T	C	14: 31,362,491 (GRCm39)	E52G	probably benign	Het
Ice2	A	G	9: 69,314,635 (GRCm39)	Y128C	probably damaging	Het
Ikzf2	T	A	1: 69,581,446 (GRCm39)	I121L	probably benign	Het
Ipcef1	C	T	10: 6,869,900 (GRCm39)	A167T	probably benign	Het
Jakmip2	T	A	18: 43,715,145 (GRCm39)	D127V	possibly damaging	Het
Jph4	T	C	14: 55,352,368 (GRCm39)	T122A	probably damaging	Het
Kcns2	A	G	15: 34,838,949 (GRCm39)	E104G	probably damaging	Het
Lars2	A	C	9: 123,260,982 (GRCm39)	R384S	probably benign	Het
Lsmem1	T	A	12: 40,235,407 (GRCm39)	H3L	possibly damaging	Het
Lsmem1	G	T	12: 40,235,408 (GRCm39)	H3N	possibly damaging	Het
Ly6g2	T	G	15: 75,088,605 (GRCm39)		probably null	Het
Mfhas1	A	C	8: 36,057,222 (GRCm39)	S566R	probably benign	Het
Mfhas1	C	G	8: 36,057,402 (GRCm39)	R626G	probably benign	Het
Mgam	A	G	6: 40,643,826 (GRCm39)	T585A	probably damaging	Het
Mmp25	T	C	17: 23,858,997 (GRCm39)	K185E	probably benign	Het
Mtch1	T	C	17: 29,557,750 (GRCm39)	I243V	probably damaging	Het
Mtcp1	A	T	X: 74,455,271 (GRCm39)	Y25*	probably null	Het
Mybl2	A	G	2: 162,901,503 (GRCm39)	T35A	probably benign	Het
Myh11	T	C	16: 14,041,744 (GRCm39)	E736G	probably damaging	Het
Myh2	T	C	11: 67,067,385 (GRCm39)	I224T	probably damaging	Het
Mymx	T	C	17: 45,912,759 (GRCm39)		probably benign	Het
Nek10	A	G	14: 14,863,454 (GRCm38)		probably null	Het
Nsd1	A	T	13: 55,394,182 (GRCm39)	K697N	probably damaging	Het
Nynrin	A	G	14: 56,110,404 (GRCm39)	D1837G	possibly damaging	Het
Or14j4	G	A	17: 37,921,168 (GRCm39)	T158I	probably benign	Het
Or2ag2b	A	G	7: 106,418,214 (GRCm39)	H308R	probably benign	Het
Or52s1	A	T	7: 102,861,093 (GRCm39)	T9S	probably benign	Het
Or5p81	T	A	7: 108,266,851 (GRCm39)	I76N	probably benign	Het
Or6k4	T	A	1: 173,964,760 (GRCm39)	I150N	probably benign	Het
Pgap6	T	C	17: 26,341,194 (GRCm39)	Y766H	probably damaging	Het
Phf19	T	C	2: 34,801,781 (GRCm39)	T10A	probably benign	Het
Pkd1	A	T	17: 24,784,558 (GRCm39)	H368L	probably benign	Het
Plscr2	A	G	9: 92,172,808 (GRCm39)	R156G	probably damaging	Het
Ppp1r42	G	A	1: 10,070,311 (GRCm39)	R61C	probably benign	Het
Pptc7	T	G	5: 122,451,679 (GRCm39)	V45G	probably damaging	Het
Prlhr	G	A	19: 60,455,867 (GRCm39)	T233I	probably damaging	Het
Prr23a4	T	A	9: 98,785,446 (GRCm39)	I37N	possibly damaging	Het
Reps1	C	T	10: 17,983,462 (GRCm39)	T435I	probably damaging	Het
Ret	T	A	6: 118,130,912 (GRCm39)	T1084S	probably damaging	Het
Rgl2	T	A	17: 34,152,595 (GRCm39)	M402K	probably benign	Het
Rp2	A	G	X: 20,243,154 (GRCm39)	K43R	probably benign	Het
Rundc3b	A	T	5: 8,629,117 (GRCm39)	W95R	probably damaging	Het
Samd9l	T	C	6: 3,375,107 (GRCm39)	D718G	possibly damaging	Het
Scnn1b	G	A	7: 121,502,068 (GRCm39)	R242H	probably benign	Het
Smad4	G	T	18: 73,774,965 (GRCm39)	Q445K	probably benign	Het
Smchd1	G	T	17: 71,677,332 (GRCm39)	P1486T	probably damaging	Het
Snx25	G	T	8: 46,488,669 (GRCm39)	N895K	possibly damaging	Het
Sox2	A	G	3: 34,704,890 (GRCm39)	D109G	probably damaging	Het
Stfa2	A	T	16: 36,225,564 (GRCm39)	N38K	probably damaging	Het
Stfa2	C	A	16: 36,225,573 (GRCm39)	E35D	possibly damaging	Het
Stfa3	A	G	16: 36,271,023 (GRCm39)	L87P	probably damaging	Het
Strbp	T	C	2: 37,530,921 (GRCm39)	D111G	possibly damaging	Het
Supt20	C	A	3: 54,635,079 (GRCm39)		probably benign	Het
Svep1	A	G	4: 58,096,310 (GRCm39)	Y1437H	possibly damaging	Het
Tbx4	T	A	11: 85,802,746 (GRCm39)		probably null	Het
Tmem117	A	T	15: 94,992,432 (GRCm39)	N364I	probably damaging	Het
Trat1	T	C	16: 48,581,742 (GRCm39)	E45G	probably damaging	Het
Trpc2	G	A	7: 101,733,326 (GRCm39)	D92N	probably damaging	Het
Trpm1	G	A	7: 63,876,530 (GRCm39)	D528N	probably damaging	Het
Ttll9	A	G	2: 152,842,156 (GRCm39)	S337G	possibly damaging	Het
Utrn	A	T	10: 12,351,018 (GRCm39)	I355N	probably damaging	Het
Vangl1	A	G	3: 102,070,782 (GRCm39)	S385P	probably benign	Het
Vmn1r209	A	G	13: 22,990,409 (GRCm39)	S94P	possibly damaging	Het
Vmn2r28	T	A	7: 5,496,810 (GRCm39)	Q14L	probably benign	Het
Vps45	A	G	3: 95,954,557 (GRCm39)		probably null	Het
Wdr48	T	C	9: 119,733,396 (GRCm39)	V81A	probably benign	Het
Xpnpep2	A	G	X: 47,214,230 (GRCm39)	N476S	probably benign	Het
Zbtb41	A	T	1: 139,374,660 (GRCm39)	K707*	probably null	Het
Zfp442	A	C	2: 150,250,983 (GRCm39)	C306W	probably damaging	Het
Zfp811	T	C	17: 33,017,116 (GRCm39)	N307S	possibly damaging	Het
Zfp976	A	G	7: 42,265,735 (GRCm39)	W17R	probably damaging	Het
Zyg11b	T	C	4: 108,123,290 (GRCm39)	T226A	possibly damaging	Het

Other mutations in Atp2b2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00802:Atp2b2	APN	6	113,782,476 (GRCm39)	missense	possibly damaging	0.69
IGL01140:Atp2b2	APN	6	113,766,932 (GRCm39)	missense	possibly damaging	0.94
IGL02065:Atp2b2	APN	6	113,790,828 (GRCm39)	missense	probably damaging	1.00
IGL02267:Atp2b2	APN	6	113,770,691 (GRCm39)	missense	probably damaging	1.00
IGL02383:Atp2b2	APN	6	113,790,903 (GRCm39)	missense	probably damaging	0.99
IGL02498:Atp2b2	APN	6	113,770,815 (GRCm39)	missense	probably damaging	0.99
IGL02631:Atp2b2	APN	6	113,725,506 (GRCm39)	missense	probably damaging	1.00
IGL03028:Atp2b2	APN	6	113,736,103 (GRCm39)	missense	probably damaging	0.99
IGL03221:Atp2b2	APN	6	113,737,820 (GRCm39)	splice site	probably benign
IGL03290:Atp2b2	APN	6	113,770,715 (GRCm39)	missense	probably damaging	1.00
johan	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
lohan	UTSW	6	113,737,611 (GRCm39)	missense	probably damaging	1.00
IGL02799:Atp2b2	UTSW	6	113,739,813 (GRCm39)	nonsense	probably null
R0116:Atp2b2	UTSW	6	113,770,656 (GRCm39)	missense	probably damaging	1.00
R0131:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0131:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0132:Atp2b2	UTSW	6	113,770,743 (GRCm39)	missense	probably damaging	1.00
R0195:Atp2b2	UTSW	6	113,770,835 (GRCm39)	missense	probably benign	0.07
R0421:Atp2b2	UTSW	6	113,790,849 (GRCm39)	missense	probably damaging	1.00
R0791:Atp2b2	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
R0792:Atp2b2	UTSW	6	113,750,349 (GRCm39)	missense	probably damaging	1.00
R1033:Atp2b2	UTSW	6	113,770,849 (GRCm39)	splice site	probably null
R1248:Atp2b2	UTSW	6	113,794,153 (GRCm39)	missense	probably damaging	1.00
R1524:Atp2b2	UTSW	6	113,751,162 (GRCm39)	splice site	probably benign
R1809:Atp2b2	UTSW	6	113,780,704 (GRCm39)	intron	probably benign
R1854:Atp2b2	UTSW	6	113,819,244 (GRCm39)	missense	probably damaging	1.00
R2127:Atp2b2	UTSW	6	113,737,611 (GRCm39)	missense	probably damaging	1.00
R2138:Atp2b2	UTSW	6	113,773,268 (GRCm39)	missense	probably benign	0.21
R2351:Atp2b2	UTSW	6	113,766,718 (GRCm39)	missense	possibly damaging	0.91
R3923:Atp2b2	UTSW	6	113,774,069 (GRCm39)	critical splice donor site	probably null
R3951:Atp2b2	UTSW	6	113,737,792 (GRCm39)	missense	possibly damaging	0.51
R4178:Atp2b2	UTSW	6	113,770,679 (GRCm39)	missense	probably damaging	1.00
R4353:Atp2b2	UTSW	6	113,742,745 (GRCm39)	missense	probably benign	0.01
R4578:Atp2b2	UTSW	6	113,737,672 (GRCm39)	missense	probably damaging	1.00
R4797:Atp2b2	UTSW	6	113,766,847 (GRCm39)	missense	possibly damaging	0.92
R4884:Atp2b2	UTSW	6	113,819,147 (GRCm39)	missense	possibly damaging	0.65
R4976:Atp2b2	UTSW	6	113,736,122 (GRCm39)	missense	probably damaging	1.00
R5273:Atp2b2	UTSW	6	113,736,193 (GRCm39)	missense	probably damaging	1.00
R5350:Atp2b2	UTSW	6	113,736,199 (GRCm39)	missense	probably damaging	0.99
R5414:Atp2b2	UTSW	6	113,819,102 (GRCm39)	missense	probably damaging	1.00
R5560:Atp2b2	UTSW	6	113,751,319 (GRCm39)	missense	possibly damaging	0.90
R5589:Atp2b2	UTSW	6	113,751,400 (GRCm39)	missense	possibly damaging	0.94
R5790:Atp2b2	UTSW	6	113,736,270 (GRCm39)	missense	probably damaging	0.97
R6001:Atp2b2	UTSW	6	113,770,728 (GRCm39)	missense	probably damaging	1.00
R6127:Atp2b2	UTSW	6	113,790,838 (GRCm39)	missense	probably damaging	1.00
R6331:Atp2b2	UTSW	6	113,774,092 (GRCm39)	missense	probably benign	0.01
R6925:Atp2b2	UTSW	6	113,737,681 (GRCm39)	missense	probably damaging	1.00
R7231:Atp2b2	UTSW	6	113,742,693 (GRCm39)	missense	possibly damaging	0.89
R8219:Atp2b2	UTSW	6	113,770,811 (GRCm39)	missense	probably damaging	1.00
R8233:Atp2b2	UTSW	6	113,742,680 (GRCm39)	critical splice donor site	probably null
R8286:Atp2b2	UTSW	6	113,819,275 (GRCm39)	missense	possibly damaging	0.64
R8369:Atp2b2	UTSW	6	113,790,747 (GRCm39)	critical splice donor site	probably null
R8444:Atp2b2	UTSW	6	113,770,772 (GRCm39)	missense	probably benign	0.18
R8942:Atp2b2	UTSW	6	113,790,991 (GRCm39)	missense	probably benign	0.00
R8953:Atp2b2	UTSW	6	113,737,630 (GRCm39)	missense	possibly damaging	0.82
R8977:Atp2b2	UTSW	6	113,750,325 (GRCm39)	missense	probably damaging	1.00
R9051:Atp2b2	UTSW	6	113,740,566 (GRCm39)	missense	probably damaging	1.00
R9399:Atp2b2	UTSW	6	113,780,713 (GRCm39)	missense	probably benign
R9648:Atp2b2	UTSW	6	113,780,707 (GRCm39)	critical splice donor site	probably null
X0020:Atp2b2	UTSW	6	113,782,461 (GRCm39)	missense	probably damaging	1.00
X0020:Atp2b2	UTSW	6	113,782,460 (GRCm39)	missense	probably damaging	1.00
Z1088:Atp2b2	UTSW	6	113,819,267 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTGATACTGTTACACGGGGAC -3'
(R):5'- GGTATTCTGGGAAGCAGATCC -3'

Sequencing Primer
(F):5'- CCTGAAACTGAGAGAGCTGAGC -3'
(R):5'- CATTTGATGACCAAGGTAGGTG -3'

Posted On

2014-06-23