Incidental Mutation 'R1831:Nek10'
| ID |
207359 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
039858-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R1831 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 14842789 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Leucine
at position 165
(M165L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000153142
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112630
AA Change: M165L
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
AA Change: M165L
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112631
AA Change: M165L
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
AA Change: M165L
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224491
AA Change: M165L
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| Meta Mutation Damage Score |
0.1140 |
| Coding Region Coverage |
- 1x: 97.5%
- 3x: 96.9%
- 10x: 95.2%
- 20x: 92.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 79 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700109H08Rik |
A |
G |
5: 3,627,255 (GRCm39) |
D77G |
probably damaging |
Het |
| Adam1b |
A |
G |
5: 121,641,000 (GRCm39) |
I15T |
possibly damaging |
Het |
| Arfgef1 |
G |
C |
1: 10,275,115 (GRCm39) |
I312M |
probably benign |
Het |
| Capn8 |
G |
A |
1: 182,438,666 (GRCm39) |
|
probably null |
Het |
| Carmil1 |
A |
T |
13: 24,348,862 (GRCm39) |
V15E |
probably benign |
Het |
| Ccdc42 |
T |
A |
11: 68,481,805 (GRCm39) |
M133K |
probably benign |
Het |
| Cd5 |
T |
C |
19: 10,696,933 (GRCm39) |
D485G |
probably damaging |
Het |
| Cdhr18 |
A |
G |
14: 13,899,619 (GRCm38) |
I101T |
probably damaging |
Het |
| Cep104 |
T |
A |
4: 154,087,003 (GRCm39) |
V842E |
probably benign |
Het |
| Cep162 |
T |
A |
9: 87,088,985 (GRCm39) |
I966L |
probably damaging |
Het |
| Cklf |
T |
C |
8: 104,977,687 (GRCm39) |
F13S |
probably damaging |
Het |
| Csf2rb |
C |
T |
15: 78,232,453 (GRCm39) |
P587S |
probably benign |
Het |
| Cyp21a1 |
A |
T |
17: 35,023,009 (GRCm39) |
|
probably benign |
Het |
| Cyp26a1 |
A |
T |
19: 37,689,071 (GRCm39) |
L335F |
probably damaging |
Het |
| Dcst1 |
A |
G |
3: 89,260,057 (GRCm39) |
F596L |
probably damaging |
Het |
| Dennd6a |
T |
A |
14: 26,328,109 (GRCm39) |
L44H |
probably damaging |
Het |
| Dnah6 |
G |
A |
6: 73,158,780 (GRCm39) |
R608C |
possibly damaging |
Het |
| Dnajb5 |
A |
T |
4: 42,957,333 (GRCm39) |
T311S |
probably benign |
Het |
| Dthd1 |
A |
G |
5: 62,984,572 (GRCm39) |
T426A |
probably benign |
Het |
| Dync1h1 |
A |
G |
12: 110,580,493 (GRCm39) |
K118R |
probably damaging |
Het |
| Efemp1 |
A |
T |
11: 28,871,442 (GRCm39) |
D347V |
possibly damaging |
Het |
| Ephb4 |
T |
A |
5: 137,352,677 (GRCm39) |
Y87N |
probably damaging |
Het |
| Ern1 |
A |
T |
11: 106,290,668 (GRCm39) |
|
probably null |
Het |
| Fam184a |
A |
T |
10: 53,523,180 (GRCm39) |
D164E |
probably damaging |
Het |
| Fkbp10 |
A |
G |
11: 100,314,045 (GRCm39) |
E351G |
probably damaging |
Het |
| Fmn2 |
A |
G |
1: 174,437,511 (GRCm39) |
S1161G |
probably benign |
Het |
| Frem1 |
A |
G |
4: 82,939,074 (GRCm39) |
S3P |
possibly damaging |
Het |
| Gpr3 |
C |
T |
4: 132,938,454 (GRCm39) |
A73T |
possibly damaging |
Het |
| Gprc6a |
T |
C |
10: 51,491,902 (GRCm39) |
T616A |
probably benign |
Het |
| Gtf3c2 |
G |
T |
5: 31,325,713 (GRCm39) |
Q452K |
probably damaging |
Het |
| H2-Q7 |
C |
A |
17: 35,658,675 (GRCm39) |
S104R |
probably benign |
Het |
| Hacd2 |
T |
C |
16: 34,922,434 (GRCm39) |
Y208H |
probably damaging |
Het |
| Hid1 |
C |
T |
11: 115,239,729 (GRCm39) |
G734R |
probably damaging |
Het |
| Ifi207 |
A |
G |
1: 173,559,992 (GRCm39) |
I160T |
unknown |
Het |
| Itga11 |
T |
A |
9: 62,689,300 (GRCm39) |
L1155Q |
probably damaging |
Het |
| Kmt2d |
A |
G |
15: 98,753,224 (GRCm39) |
S157P |
probably damaging |
Het |
| Lamb3 |
A |
G |
1: 193,017,187 (GRCm39) |
T793A |
probably damaging |
Het |
| Lonrf2 |
G |
A |
1: 38,852,357 (GRCm39) |
P165S |
probably benign |
Het |
| Lrrc66 |
G |
A |
5: 73,764,769 (GRCm39) |
S758L |
possibly damaging |
Het |
| Ltn1 |
C |
T |
16: 87,197,034 (GRCm39) |
S1213N |
possibly damaging |
Het |
| Meak7 |
T |
C |
8: 120,497,992 (GRCm39) |
M171V |
probably null |
Het |
| Med1 |
T |
C |
11: 98,047,437 (GRCm39) |
|
probably benign |
Het |
| Megf6 |
C |
T |
4: 154,355,134 (GRCm39) |
T1483M |
probably benign |
Het |
| Micall2 |
A |
G |
5: 139,702,508 (GRCm39) |
V245A |
probably benign |
Het |
| Mipep |
T |
G |
14: 61,109,512 (GRCm39) |
Y630D |
probably damaging |
Het |
| Ndst3 |
T |
A |
3: 123,395,127 (GRCm39) |
H501L |
probably benign |
Het |
| Nmbr |
C |
A |
10: 14,642,609 (GRCm39) |
T56K |
probably benign |
Het |
| Nxpe2 |
T |
A |
9: 48,237,452 (GRCm39) |
M268L |
probably benign |
Het |
| Oasl2 |
A |
G |
5: 115,039,367 (GRCm39) |
Y185C |
probably benign |
Het |
| Ogdhl |
T |
A |
14: 32,059,484 (GRCm39) |
V377E |
probably damaging |
Het |
| Or11g1 |
T |
A |
14: 50,651,658 (GRCm39) |
|
probably null |
Het |
| Or14j4 |
G |
A |
17: 37,920,730 (GRCm39) |
S304L |
possibly damaging |
Het |
| Ovgp1 |
A |
G |
3: 105,892,384 (GRCm39) |
R346G |
probably benign |
Het |
| Parp14 |
T |
A |
16: 35,678,958 (GRCm39) |
N337Y |
possibly damaging |
Het |
| Pask |
A |
C |
1: 93,248,491 (GRCm39) |
|
probably null |
Het |
| Pax3 |
G |
T |
1: 78,108,977 (GRCm39) |
T227K |
probably damaging |
Het |
| Pik3r6 |
T |
A |
11: 68,434,860 (GRCm39) |
M594K |
probably benign |
Het |
| Pms1 |
A |
G |
1: 53,246,370 (GRCm39) |
F390L |
probably benign |
Het |
| Polg |
G |
A |
7: 79,109,518 (GRCm39) |
T433I |
probably benign |
Het |
| Prex1 |
T |
C |
2: 166,427,021 (GRCm39) |
Y898C |
probably damaging |
Het |
| Ranbp2 |
T |
A |
10: 58,315,044 (GRCm39) |
C1921* |
probably null |
Het |
| Rif1 |
T |
A |
2: 51,968,507 (GRCm39) |
L230* |
probably null |
Het |
| Rnf148 |
A |
G |
6: 23,654,772 (GRCm39) |
F75L |
probably damaging |
Het |
| Rps18-ps6 |
A |
G |
13: 97,897,053 (GRCm39) |
V15A |
probably benign |
Het |
| Sclt1 |
A |
G |
3: 41,681,546 (GRCm39) |
V91A |
probably damaging |
Het |
| Sirt1 |
T |
C |
10: 63,156,425 (GRCm39) |
D735G |
probably benign |
Het |
| Spag5 |
T |
A |
11: 78,205,082 (GRCm39) |
N622K |
probably benign |
Het |
| Sspo |
T |
G |
6: 48,466,720 (GRCm39) |
C3935W |
probably damaging |
Het |
| Strbp |
A |
G |
2: 37,515,277 (GRCm39) |
S250P |
possibly damaging |
Het |
| Tgfbr2 |
T |
C |
9: 115,919,604 (GRCm39) |
T541A |
possibly damaging |
Het |
| Thada |
A |
C |
17: 84,538,542 (GRCm39) |
S1489A |
probably damaging |
Het |
| Tiam1 |
A |
T |
16: 89,657,182 (GRCm39) |
S685T |
probably benign |
Het |
| Tpsb2 |
T |
A |
17: 25,585,494 (GRCm39) |
|
probably null |
Het |
| Trip4 |
C |
A |
9: 65,765,622 (GRCm39) |
G359V |
probably damaging |
Het |
| Tsr1 |
T |
C |
11: 74,791,182 (GRCm39) |
F254L |
probably benign |
Het |
| Vmn1r120 |
T |
C |
7: 20,787,556 (GRCm39) |
K52E |
probably benign |
Het |
| Vmn1r29 |
C |
A |
6: 58,284,692 (GRCm39) |
Y137* |
probably null |
Het |
| Vmn2r52 |
T |
C |
7: 9,893,415 (GRCm39) |
K575E |
probably damaging |
Het |
| Wdr95 |
A |
G |
5: 149,475,891 (GRCm39) |
Y63C |
probably damaging |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R0633:Nek10
|
UTSW |
14 |
14,857,782 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6235:Nek10
|
UTSW |
14 |
14,821,113 (GRCm38) |
nonsense |
probably null |
|
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7202:Nek10
|
UTSW |
14 |
14,836,171 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7251:Nek10
|
UTSW |
14 |
14,853,965 (GRCm38) |
missense |
probably benign |
|
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9464:Nek10
|
UTSW |
14 |
14,937,766 (GRCm38) |
missense |
probably benign |
|
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ACTAAGTCCTGTGTCCCACC -3'
(R):5'- GCTTGCCAAAGAAAGAGCTGC -3'
Sequencing Primer
(F):5'- ACCCTTGAGTGCCACAGG -3'
(R):5'- GACATGTGATACCATTGGCAC -3'
|
| Posted On |
2014-06-23 |
Incidental Mutation