Mutagenetix > Incidental Mutation

Incidental Mutation 'R1848:Srrt'

207873

Institutional Source

Beutler Lab

Gene Symbol

Srrt

Ensembl Gene

ENSMUSG00000037364

Gene Name

serrate RNA effector molecule homolog (Arabidopsis)

Synonyms

2810019G02Rik, Asr2, Ars2

MMRRC Submission

039873-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1848 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

137295704-137307674 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 137296945 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glutamine at position 308 (E308Q)

Ref Sequence

ENSEMBL: ENSMUSP00000142351 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024099] [ENSMUST00000040873] [ENSMUST00000052825] [ENSMUST00000085934] [ENSMUST00000196109] [ENSMUST00000196208] [ENSMUST00000199243] [ENSMUST00000197466] [ENSMUST00000198526]

AlphaFold

Q99MR6

Predicted Effect

probably benign
Transcript: ENSMUST00000024099

SMART Domains

Protein: ENSMUSP00000024099
Gene: ENSMUSG00000023328

Domain	Start	End	E-Value	Type
Pfam:COesterase	14	563	2e-186	PFAM
Pfam:Abhydrolase_3	146	276	7.5e-9	PFAM
Pfam:AChE_tetra	578	614	3.2e-26	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000040873
AA Change: E666Q

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000043123
Gene: ENSMUSG00000037364
AA Change: E666Q

Domain	Start	End	E-Value	Type
low complexity region	3	65	N/A	INTRINSIC
low complexity region	95	116	N/A	INTRINSIC
Pfam:DUF3546	153	262	3.8e-44	PFAM
low complexity region	269	276	N/A	INTRINSIC
low complexity region	326	350	N/A	INTRINSIC
coiled coil region	367	402	N/A	INTRINSIC
Blast:RRM	421	491	2e-31	BLAST
low complexity region	566	595	N/A	INTRINSIC
low complexity region	603	615	N/A	INTRINSIC
Pfam:ARS2	645	850	9.7e-94	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000052825

SMART Domains

Protein: ENSMUSP00000056156
Gene: ENSMUSG00000051502

Domain	Start	End	E-Value	Type
Pfam:Peptidase_C78	27	212	5.4e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000085934

SMART Domains

Protein: ENSMUSP00000083097
Gene: ENSMUSG00000023328

Domain	Start	End	E-Value	Type
Pfam:COesterase	15	563	3e-178	PFAM
Pfam:Abhydrolase_3	146	260	1.4e-7	PFAM
Pfam:AChE_tetra	578	613	3.2e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150983

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184134

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184197

Predicted Effect

probably damaging
Transcript: ENSMUST00000196109
AA Change: E308Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000142351
Gene: ENSMUSG00000037364
AA Change: E308Q

Domain	Start	End	E-Value	Type
coiled coil region	11	46	N/A	INTRINSIC
Blast:RRM	65	133	2e-15	BLAST
low complexity region	208	237	N/A	INTRINSIC
low complexity region	245	257	N/A	INTRINSIC
Pfam:ARS2	277	498	6.5e-111	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000196208

SMART Domains

Protein: ENSMUSP00000142427
Gene: ENSMUSG00000023328

Domain	Start	End	E-Value	Type
Pfam:COesterase	14	359	6.5e-134	PFAM
Pfam:Abhydrolase_3	146	284	4.1e-7	PFAM
Pfam:COesterase	355	475	1.5e-25	PFAM
Pfam:AChE_tetra	490	526	2.2e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196394

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196576

Predicted Effect

probably damaging
Transcript: ENSMUST00000199243
AA Change: E666Q

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000143232
Gene: ENSMUSG00000037364
AA Change: E666Q

Domain	Start	End	E-Value	Type
low complexity region	3	65	N/A	INTRINSIC
low complexity region	95	116	N/A	INTRINSIC
Pfam:DUF3546	151	264	9.5e-48	PFAM
low complexity region	269	276	N/A	INTRINSIC
low complexity region	326	350	N/A	INTRINSIC
coiled coil region	367	402	N/A	INTRINSIC
Blast:RRM	421	491	2e-31	BLAST
low complexity region	566	595	N/A	INTRINSIC
low complexity region	603	615	N/A	INTRINSIC
Pfam:ARS2	635	849	9.8e-115	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000197466
AA Change: E666Q

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000142564
Gene: ENSMUSG00000037364
AA Change: E666Q

Domain	Start	End	E-Value	Type
low complexity region	3	65	N/A	INTRINSIC
low complexity region	95	116	N/A	INTRINSIC
Pfam:DUF3546	151	264	9.5e-48	PFAM
low complexity region	269	276	N/A	INTRINSIC
low complexity region	326	350	N/A	INTRINSIC
coiled coil region	367	402	N/A	INTRINSIC
Blast:RRM	421	491	2e-31	BLAST
low complexity region	566	595	N/A	INTRINSIC
low complexity region	603	615	N/A	INTRINSIC
Pfam:ARS2	635	845	5.5e-113	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000199756
AA Change: E88Q

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200643

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197144

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196960

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197376

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197409

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199473

Predicted Effect

probably benign
Transcript: ENSMUST00000223263

Predicted Effect

probably benign
Transcript: ENSMUST00000199605

Predicted Effect

probably benign
Transcript: ENSMUST00000199365

Predicted Effect

probably benign
Transcript: ENSMUST00000198526

SMART Domains

Protein: ENSMUSP00000142435
Gene: ENSMUSG00000037364

Domain	Start	End	E-Value	Type
low complexity region	3	65	N/A	INTRINSIC
low complexity region	95	116	N/A	INTRINSIC
Pfam:DUF3546	151	264	2e-45	PFAM
low complexity region	269	276	N/A	INTRINSIC
low complexity region	322	347	N/A	INTRINSIC
low complexity region	369	408	N/A	INTRINSIC

Meta Mutation Damage Score

0.3713

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.2%

Validation Efficiency

98% (120/123)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele display embryonic lethality before somite formation, increased apoptosis, and when cultured most fail to hatch from the zona pellucida. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 120 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402J07Rik	C	A	8: 87,568,493 (GRCm38)	Y86*	probably null	Het
Aadac	A	G	3: 60,039,697 (GRCm38)	E272G	probably damaging	Het
Abcc8	A	T	7: 46,166,902 (GRCm38)	D271E	probably benign	Het
Acan	T	C	7: 79,099,035 (GRCm38)	F1185L	probably benign	Het
Adam1a	A	T	5: 121,519,620 (GRCm38)	C537S	probably damaging	Het
Ago2	A	G	15: 73,123,965 (GRCm38)	V395A	probably benign	Het
Alox15	A	G	11: 70,350,752 (GRCm38)	V101A	probably damaging	Het
Ankra2	T	C	13: 98,271,124 (GRCm38)	I194T	probably damaging	Het
Apobec4	C	A	1: 152,756,230 (GRCm38)	P3H	probably damaging	Het
Arid3b	G	T	9: 57,796,677 (GRCm38)	Y329*	probably null	Het
Atm	A	T	9: 53,468,012 (GRCm38)	S1993T	probably benign	Het
Bpgm	T	G	6: 34,487,734 (GRCm38)	S129A	probably benign	Het
Brat1	A	G	5: 140,718,509 (GRCm38)	D839G	possibly damaging	Het
Ccdc15	A	T	9: 37,342,570 (GRCm38)	S128T	probably benign	Het
Cd300lg	A	T	11: 102,046,206 (GRCm38)		probably benign	Het
Cdc34b	C	A	11: 94,742,477 (GRCm38)	Q168K	probably damaging	Het
Celsr2	A	T	3: 108,401,310 (GRCm38)	V1767E	probably benign	Het
Cep350	T	C	1: 155,953,651 (GRCm38)	D169G	probably benign	Het
Col7a1	A	T	9: 108,969,565 (GRCm38)	D1762V	possibly damaging	Het
Coro7	A	G	16: 4,630,434 (GRCm38)	L724P	probably damaging	Het
Crb1	T	A	1: 139,237,012 (GRCm38)	I1125F	probably damaging	Het
Ctif	T	A	18: 75,519,941 (GRCm38)	D415V	probably damaging	Het
Dab1	C	T	4: 104,731,751 (GRCm38)	A524V	probably benign	Het
Dhrs2	A	G	14: 55,240,841 (GRCm38)	D237G	probably benign	Het
Dhx9	T	C	1: 153,465,753 (GRCm38)	Q582R	probably damaging	Het
Dnajc1	C	T	2: 18,219,713 (GRCm38)	R443Q	probably damaging	Het
Dnm2	C	T	9: 21,505,681 (GRCm38)	R837W	possibly damaging	Het
Dpf2	C	A	19: 5,906,615 (GRCm38)	Q70H	probably damaging	Het
Dqx1	T	A	6: 83,066,107 (GRCm38)	D608E	probably damaging	Het
Dync2h1	T	A	9: 7,049,166 (GRCm38)	T3245S	probably benign	Het
Ect2l	A	G	10: 18,200,033 (GRCm38)	L35P	probably damaging	Het
Efcab5	A	T	11: 77,103,306 (GRCm38)	L1285Q	probably damaging	Het
Eif4g1	G	C	16: 20,681,867 (GRCm38)	R697P	probably damaging	Het
Emsy	T	A	7: 98,600,821 (GRCm38)	E753V	probably damaging	Het
Entpd3	A	G	9: 120,558,419 (GRCm38)	I227M	probably damaging	Het
Epn1	T	A	7: 5,089,998 (GRCm38)	V103E	probably damaging	Het
Esrra	T	C	19: 6,912,010 (GRCm38)	D337G	probably benign	Het
Fam129c	T	C	8: 71,603,769 (GRCm38)	M371T	possibly damaging	Het
Fam83e	A	T	7: 45,728,769 (GRCm38)	K406*	probably null	Het
Fam83e	A	T	7: 45,728,770 (GRCm38)	K406M	possibly damaging	Het
Fat2	A	G	11: 55,311,558 (GRCm38)	I230T	probably damaging	Het
Fbxl16	A	G	17: 25,816,446 (GRCm38)	I6V	probably benign	Het
Fgf23	T	C	6: 127,073,193 (GRCm38)	I55T	probably damaging	Het
Fibcd1	T	A	2: 31,821,549 (GRCm38)	D288V	probably damaging	Het
Flnb	T	C	14: 7,892,113 (GRCm38)	I594T	probably damaging	Het
Gabbr2	T	C	4: 46,739,823 (GRCm38)	E449G	probably benign	Het
Gbf1	T	G	19: 46,272,037 (GRCm38)	S1130A	possibly damaging	Het
Gipc3	T	C	10: 81,341,265 (GRCm38)	E157G	probably damaging	Het
Glra3	G	T	8: 55,940,907 (GRCm38)	A18S	probably benign	Het
Gm6625	A	C	8: 89,146,834 (GRCm38)		noncoding transcript	Het
Gpx4	A	G	10: 80,056,036 (GRCm38)		probably benign	Het
Grb10	A	G	11: 11,946,029 (GRCm38)	F264L	possibly damaging	Het
Grik3	T	C	4: 125,694,138 (GRCm38)	Y684H	probably damaging	Het
Gstp1	C	T	19: 4,036,795 (GRCm38)		probably benign	Het
Haus8	G	A	8: 71,256,123 (GRCm38)		probably benign	Het
Hip1	G	A	5: 135,435,141 (GRCm38)		probably null	Het
Hist3h2ba	A	T	11: 58,949,102 (GRCm38)	I55F	possibly damaging	Het
Hspbap1	T	A	16: 35,818,764 (GRCm38)		probably null	Het
Htr2b	T	A	1: 86,099,429 (GRCm38)	I452F	possibly damaging	Het
Hydin	T	A	8: 110,569,808 (GRCm38)	H3656Q	probably benign	Het
Klb	T	A	5: 65,348,837 (GRCm38)	D142E	probably benign	Het
Lamb3	A	T	1: 193,334,616 (GRCm38)	T777S	possibly damaging	Het
Lins1	C	A	7: 66,714,322 (GRCm38)	T650K	probably damaging	Het
Loxhd1	A	G	18: 77,281,971 (GRCm38)	K5R	possibly damaging	Het
Lpp	G	A	16: 24,761,655 (GRCm38)	M40I	probably damaging	Het
Mia2	A	T	12: 59,170,251 (GRCm38)		probably benign	Het
Miip	A	C	4: 147,863,092 (GRCm38)	F204V	probably damaging	Het
Mmp21	T	C	7: 133,677,153 (GRCm38)	R323G	probably benign	Het
Mta3	T	A	17: 83,755,551 (GRCm38)		probably benign	Het
Myh1	A	G	11: 67,213,630 (GRCm38)	K1004R	probably benign	Het
Myh14	T	A	7: 44,632,429 (GRCm38)	I810F	probably damaging	Het
Nbas	A	C	12: 13,413,597 (GRCm38)	D1295A	probably damaging	Het
Npr2	T	G	4: 43,632,384 (GRCm38)	V67G	probably benign	Het
Oas1f	C	A	5: 120,855,429 (GRCm38)	Q235K	probably damaging	Het
Olfr109	T	A	17: 37,467,047 (GRCm38)	S280R	probably damaging	Het
Olfr59	G	T	11: 74,289,213 (GRCm38)	C189F	probably damaging	Het
Olfr60	T	A	7: 140,345,987 (GRCm38)	M1L	probably benign	Het
Olfr629	T	C	7: 103,741,174 (GRCm38)	N22S	probably benign	Het
Olfr857	C	T	9: 19,713,090 (GRCm38)	H88Y	probably benign	Het
Pafah2	GCCCC	GCCCCC	4: 134,425,541 (GRCm38)		probably null	Het
Pde1b	A	G	15: 103,525,340 (GRCm38)		probably null	Het
Pdilt	T	C	7: 119,489,384 (GRCm38)	T465A	probably benign	Het
Plxnd1	T	C	6: 115,966,546 (GRCm38)	H1233R	probably damaging	Het
Ppm1b	T	A	17: 84,994,124 (GRCm38)	M144K	probably benign	Het
Prkdc	T	C	16: 15,808,058 (GRCm38)	L3316S	probably benign	Het
Prm2	T	A	16: 10,791,591 (GRCm38)		probably benign	Het
Prmt7	T	C	8: 106,237,008 (GRCm38)	V240A	probably benign	Het
Prx	C	A	7: 27,518,888 (GRCm38)	A938E	possibly damaging	Het
Rbm7	A	G	9: 48,490,894 (GRCm38)	V131A	probably benign	Het
Ric1	T	A	19: 29,600,813 (GRCm38)		probably null	Het
Rnf150	A	T	8: 82,864,010 (GRCm38)	M1L	possibly damaging	Het
Rnf20	C	T	4: 49,644,628 (GRCm38)	R298W	probably damaging	Het
Rp1	T	A	1: 4,347,232 (GRCm38)	Y1219F	possibly damaging	Het
Scn7a	A	G	2: 66,684,013 (GRCm38)		probably null	Het
Sdad1	A	G	5: 92,292,651 (GRCm38)		probably null	Het
Sept9	T	C	11: 117,353,083 (GRCm38)		probably benign	Het
Serinc3	T	C	2: 163,645,489 (GRCm38)		probably benign	Het
Shc3	C	T	13: 51,461,388 (GRCm38)	G178R	probably damaging	Het
Slc4a10	A	T	2: 62,316,606 (GRCm38)	K1090M	probably damaging	Het
Slco1a1	T	C	6: 141,923,111 (GRCm38)	I376V	probably benign	Het
Slmap	A	T	14: 26,422,574 (GRCm38)	F719L	probably benign	Het
Smgc	A	G	15: 91,859,758 (GRCm38)	N573D	possibly damaging	Het
Spx	G	A	6: 142,414,079 (GRCm38)		probably null	Het
Tas2r130	T	A	6: 131,630,597 (GRCm38)	R78S	probably benign	Het
Tchhl1	A	G	3: 93,471,101 (GRCm38)	R371G	probably damaging	Het
Teddm2	C	T	1: 153,850,448 (GRCm38)	A174T	probably benign	Het
Tktl2	G	A	8: 66,512,347 (GRCm38)	V186M	probably damaging	Het
Trim33	T	C	3: 103,324,640 (GRCm38)		probably benign	Het
Tspan31	A	G	10: 127,069,458 (GRCm38)	V40A	probably damaging	Het
Uevld	A	G	7: 46,945,227 (GRCm38)		probably benign	Het
Vcl	G	T	14: 21,008,995 (GRCm38)	A560S	probably benign	Het
Vmn2r24	T	A	6: 123,816,224 (GRCm38)	C837S	probably damaging	Het
Vmn2r57	C	T	7: 41,428,107 (GRCm38)	V212M	probably damaging	Het
Vps13c	A	G	9: 67,936,340 (GRCm38)	T1968A	probably benign	Het
Vtn	A	G	11: 78,500,567 (GRCm38)	R269G	probably damaging	Het
Wdcp	G	A	12: 4,850,245 (GRCm38)	V34I	possibly damaging	Het
Zc3h14	A	G	12: 98,752,832 (GRCm38)	D152G	possibly damaging	Het
Zfp189	C	T	4: 49,529,266 (GRCm38)	P123L	probably benign	Het
Zfp318	T	C	17: 46,406,055 (GRCm38)	S1038P	possibly damaging	Het
Zfp873	A	G	10: 82,060,572 (GRCm38)	D416G	probably benign	Het

Other mutations in Srrt

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00337:Srrt	APN	5	137,295,978 (GRCm38)	unclassified	probably benign
IGL01062:Srrt	APN	5	137,296,307 (GRCm38)	missense	probably damaging	1.00
IGL02227:Srrt	APN	5	137,296,274 (GRCm38)	missense	probably damaging	1.00
IGL02656:Srrt	APN	5	137,299,676 (GRCm38)	unclassified	probably benign
IGL03105:Srrt	APN	5	137,299,844 (GRCm38)	missense	possibly damaging	0.72
IGL03137:Srrt	APN	5	137,296,117 (GRCm38)	unclassified	probably benign
R0281:Srrt	UTSW	5	137,296,127 (GRCm38)	unclassified	probably benign
R0322:Srrt	UTSW	5	137,296,608 (GRCm38)	missense	probably damaging	1.00
R0347:Srrt	UTSW	5	137,299,676 (GRCm38)	unclassified	probably benign
R1253:Srrt	UTSW	5	137,300,336 (GRCm38)	missense	probably benign	0.01
R1397:Srrt	UTSW	5	137,300,261 (GRCm38)	missense	possibly damaging	0.89
R1520:Srrt	UTSW	5	137,298,766 (GRCm38)	missense	probably damaging	0.99
R1561:Srrt	UTSW	5	137,300,019 (GRCm38)	missense	probably benign	0.24
R1645:Srrt	UTSW	5	137,302,139 (GRCm38)	nonsense	probably null
R1759:Srrt	UTSW	5	137,302,950 (GRCm38)	missense	probably damaging	1.00
R1770:Srrt	UTSW	5	137,299,860 (GRCm38)	unclassified	probably benign
R1795:Srrt	UTSW	5	137,303,012 (GRCm38)	unclassified	probably benign
R3838:Srrt	UTSW	5	137,302,125 (GRCm38)	critical splice donor site	probably null
R5015:Srrt	UTSW	5	137,296,009 (GRCm38)	missense	probably damaging	1.00
R5068:Srrt	UTSW	5	137,296,541 (GRCm38)	missense	possibly damaging	0.93
R5163:Srrt	UTSW	5	137,296,773 (GRCm38)	critical splice donor site	probably null
R5316:Srrt	UTSW	5	137,296,551 (GRCm38)	missense	probably benign	0.16
R5343:Srrt	UTSW	5	137,297,165 (GRCm38)	missense	probably damaging	1.00
R5351:Srrt	UTSW	5	137,298,284 (GRCm38)	makesense	probably null
R5412:Srrt	UTSW	5	137,296,287 (GRCm38)	missense	probably damaging	1.00
R5806:Srrt	UTSW	5	137,297,917 (GRCm38)	missense	probably damaging	0.98
R6470:Srrt	UTSW	5	137,302,656 (GRCm38)	missense	probably damaging	1.00
R6497:Srrt	UTSW	5	137,297,506 (GRCm38)	missense	probably damaging	1.00
R6755:Srrt	UTSW	5	137,302,930 (GRCm38)	missense	probably damaging	1.00
R6828:Srrt	UTSW	5	137,296,968 (GRCm38)	missense	probably damaging	1.00
R6875:Srrt	UTSW	5	137,298,673 (GRCm38)	missense	probably benign	0.00
R7586:Srrt	UTSW	5	137,302,195 (GRCm38)	missense	probably damaging	0.98
R7677:Srrt	UTSW	5	137,300,148 (GRCm38)	missense	probably damaging	0.99
R8028:Srrt	UTSW	5	137,302,498 (GRCm38)	critical splice donor site	probably benign
R8413:Srrt	UTSW	5	137,300,327 (GRCm38)	missense	possibly damaging	0.84
R8438:Srrt	UTSW	5	137,303,000 (GRCm38)	missense	unknown
R8795:Srrt	UTSW	5	137,299,976 (GRCm38)	missense	probably benign	0.17
R8925:Srrt	UTSW	5	137,298,808 (GRCm38)	missense	probably benign	0.26
R8927:Srrt	UTSW	5	137,298,808 (GRCm38)	missense	probably benign	0.26
R9024:Srrt	UTSW	5	137,303,029 (GRCm38)	missense	unknown
R9632:Srrt	UTSW	5	137,298,427 (GRCm38)	missense	possibly damaging	0.79
R9667:Srrt	UTSW	5	137,297,470 (GRCm38)	missense	probably damaging	0.96
R9793:Srrt	UTSW	5	137,296,573 (GRCm38)	missense	probably benign	0.37
RF018:Srrt	UTSW	5	137,300,000 (GRCm38)	missense	probably benign	0.23
Z1176:Srrt	UTSW	5	137,298,227 (GRCm38)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CAGCTTCCTCCAGGCTTTTAGG -3'
(R):5'- TGGCATAATCCACGTTCGG -3'

Sequencing Primer
(F):5'- ACATCTCAGACCTTGAATTTCTTG -3'
(R):5'- ATAATCCACGTTCGGGGGCC -3'

Posted On

2014-06-23