Mutagenetix > Incidental Mutation

Incidental Mutation 'R1848:Plxnd1'

207877

Institutional Source

Beutler Lab

Gene Symbol

Plxnd1

Ensembl Gene

ENSMUSG00000030123

Gene Name

plexin D1

Synonyms

b2b553Clo, 6230425C21Rik, b2b1863Clo

MMRRC Submission

039873-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1848 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

115954811-115995005 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 115966546 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 1233 (H1233R)

Ref Sequence

ENSEMBL: ENSMUSP00000015511 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015511]

AlphaFold

Q3UH93

Predicted Effect

probably damaging
Transcript: ENSMUST00000015511
AA Change: H1233R

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000015511
Gene: ENSMUSG00000030123
AA Change: H1233R

Domain	Start	End	E-Value	Type
signal peptide	1	48	N/A	INTRINSIC
Sema	61	531	6.52e-90	SMART
PSI	550	603	6.06e-12	SMART
PSI	703	755	1.06e-2	SMART
Blast:PSI	850	891	9e-20	BLAST
IPT	892	981	4.43e-20	SMART
IPT	982	1068	6.61e-19	SMART
IPT	1070	1149	6.13e-14	SMART
transmembrane domain	1271	1293	N/A	INTRINSIC
Pfam:Plexin_cytopl	1345	1888	5e-238	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000131590

SMART Domains

Protein: ENSMUSP00000115650
Gene: ENSMUSG00000030123

Domain	Start	End	E-Value	Type
Blast:PSI	2	34	1e-13	BLAST
IPT	35	124	4.43e-20	SMART
Blast:IPT	125	177	3e-30	BLAST
Pfam:TIG	180	233	4.6e-6	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.2%

Validation Efficiency

98% (120/123)

MGI Phenotype

PHENOTYPE: Homozygous null mice display neonatal lethality, thin-walled atria, and vascular abnormalities including abnormal branchial arch artery development, cardiac outflow tract abnormalities, and reduced vascular smooth muscle around some vessels. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 120 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402J07Rik	C	A	8: 87,568,493 (GRCm38)	Y86*	probably null	Het
Aadac	A	G	3: 60,039,697 (GRCm38)	E272G	probably damaging	Het
Abcc8	A	T	7: 46,166,902 (GRCm38)	D271E	probably benign	Het
Acan	T	C	7: 79,099,035 (GRCm38)	F1185L	probably benign	Het
Adam1a	A	T	5: 121,519,620 (GRCm38)	C537S	probably damaging	Het
Ago2	A	G	15: 73,123,965 (GRCm38)	V395A	probably benign	Het
Alox15	A	G	11: 70,350,752 (GRCm38)	V101A	probably damaging	Het
Ankra2	T	C	13: 98,271,124 (GRCm38)	I194T	probably damaging	Het
Apobec4	C	A	1: 152,756,230 (GRCm38)	P3H	probably damaging	Het
Arid3b	G	T	9: 57,796,677 (GRCm38)	Y329*	probably null	Het
Atm	A	T	9: 53,468,012 (GRCm38)	S1993T	probably benign	Het
Bpgm	T	G	6: 34,487,734 (GRCm38)	S129A	probably benign	Het
Brat1	A	G	5: 140,718,509 (GRCm38)	D839G	possibly damaging	Het
Ccdc15	A	T	9: 37,342,570 (GRCm38)	S128T	probably benign	Het
Cd300lg	A	T	11: 102,046,206 (GRCm38)		probably benign	Het
Cdc34b	C	A	11: 94,742,477 (GRCm38)	Q168K	probably damaging	Het
Celsr2	A	T	3: 108,401,310 (GRCm38)	V1767E	probably benign	Het
Cep350	T	C	1: 155,953,651 (GRCm38)	D169G	probably benign	Het
Col7a1	A	T	9: 108,969,565 (GRCm38)	D1762V	possibly damaging	Het
Coro7	A	G	16: 4,630,434 (GRCm38)	L724P	probably damaging	Het
Crb1	T	A	1: 139,237,012 (GRCm38)	I1125F	probably damaging	Het
Ctif	T	A	18: 75,519,941 (GRCm38)	D415V	probably damaging	Het
Dab1	C	T	4: 104,731,751 (GRCm38)	A524V	probably benign	Het
Dhrs2	A	G	14: 55,240,841 (GRCm38)	D237G	probably benign	Het
Dhx9	T	C	1: 153,465,753 (GRCm38)	Q582R	probably damaging	Het
Dnajc1	C	T	2: 18,219,713 (GRCm38)	R443Q	probably damaging	Het
Dnm2	C	T	9: 21,505,681 (GRCm38)	R837W	possibly damaging	Het
Dpf2	C	A	19: 5,906,615 (GRCm38)	Q70H	probably damaging	Het
Dqx1	T	A	6: 83,066,107 (GRCm38)	D608E	probably damaging	Het
Dync2h1	T	A	9: 7,049,166 (GRCm38)	T3245S	probably benign	Het
Ect2l	A	G	10: 18,200,033 (GRCm38)	L35P	probably damaging	Het
Efcab5	A	T	11: 77,103,306 (GRCm38)	L1285Q	probably damaging	Het
Eif4g1	G	C	16: 20,681,867 (GRCm38)	R697P	probably damaging	Het
Emsy	T	A	7: 98,600,821 (GRCm38)	E753V	probably damaging	Het
Entpd3	A	G	9: 120,558,419 (GRCm38)	I227M	probably damaging	Het
Epn1	T	A	7: 5,089,998 (GRCm38)	V103E	probably damaging	Het
Esrra	T	C	19: 6,912,010 (GRCm38)	D337G	probably benign	Het
Fam83e	A	T	7: 45,728,769 (GRCm38)	K406*	probably null	Het
Fam83e	A	T	7: 45,728,770 (GRCm38)	K406M	possibly damaging	Het
Fat2	A	G	11: 55,311,558 (GRCm38)	I230T	probably damaging	Het
Fbxl16	A	G	17: 25,816,446 (GRCm38)	I6V	probably benign	Het
Fgf23	T	C	6: 127,073,193 (GRCm38)	I55T	probably damaging	Het
Fibcd1	T	A	2: 31,821,549 (GRCm38)	D288V	probably damaging	Het
Flnb	T	C	14: 7,892,113 (GRCm38)	I594T	probably damaging	Het
Gabbr2	T	C	4: 46,739,823 (GRCm38)	E449G	probably benign	Het
Gbf1	T	G	19: 46,272,037 (GRCm38)	S1130A	possibly damaging	Het
Gipc3	T	C	10: 81,341,265 (GRCm38)	E157G	probably damaging	Het
Glra3	G	T	8: 55,940,907 (GRCm38)	A18S	probably benign	Het
Gm6625	A	C	8: 89,146,834 (GRCm38)		noncoding transcript	Het
Gpx4	A	G	10: 80,056,036 (GRCm38)		probably benign	Het
Grb10	A	G	11: 11,946,029 (GRCm38)	F264L	possibly damaging	Het
Grik3	T	C	4: 125,694,138 (GRCm38)	Y684H	probably damaging	Het
Gstp1	C	T	19: 4,036,795 (GRCm38)		probably benign	Het
Haus8	G	A	8: 71,256,123 (GRCm38)		probably benign	Het
Hip1	G	A	5: 135,435,141 (GRCm38)		probably null	Het
Hist3h2ba	A	T	11: 58,949,102 (GRCm38)	I55F	possibly damaging	Het
Hspbap1	T	A	16: 35,818,764 (GRCm38)		probably null	Het
Htr2b	T	A	1: 86,099,429 (GRCm38)	I452F	possibly damaging	Het
Hydin	T	A	8: 110,569,808 (GRCm38)	H3656Q	probably benign	Het
Klb	T	A	5: 65,348,837 (GRCm38)	D142E	probably benign	Het
Lamb3	A	T	1: 193,334,616 (GRCm38)	T777S	possibly damaging	Het
Lins1	C	A	7: 66,714,322 (GRCm38)	T650K	probably damaging	Het
Loxhd1	A	G	18: 77,281,971 (GRCm38)	K5R	possibly damaging	Het
Lpp	G	A	16: 24,761,655 (GRCm38)	M40I	probably damaging	Het
Mia2	A	T	12: 59,170,251 (GRCm38)		probably benign	Het
Miip	A	C	4: 147,863,092 (GRCm38)	F204V	probably damaging	Het
Mmp21	T	C	7: 133,677,153 (GRCm38)	R323G	probably benign	Het
Mta3	T	A	17: 83,755,551 (GRCm38)		probably benign	Het
Myh1	A	G	11: 67,213,630 (GRCm38)	K1004R	probably benign	Het
Myh14	T	A	7: 44,632,429 (GRCm38)	I810F	probably damaging	Het
Nbas	A	C	12: 13,413,597 (GRCm38)	D1295A	probably damaging	Het
Niban3	T	C	8: 71,603,769 (GRCm38)	M371T	possibly damaging	Het
Npr2	T	G	4: 43,632,384 (GRCm38)	V67G	probably benign	Het
Oas1f	C	A	5: 120,855,429 (GRCm38)	Q235K	probably damaging	Het
Or12d17	T	A	17: 37,467,047 (GRCm38)	S280R	probably damaging	Het
Or13a27	T	A	7: 140,345,987 (GRCm38)	M1L	probably benign	Het
Or1p1	G	T	11: 74,289,213 (GRCm38)	C189F	probably damaging	Het
Or52ae9	T	C	7: 103,741,174 (GRCm38)	N22S	probably benign	Het
Or7e166	C	T	9: 19,713,090 (GRCm38)	H88Y	probably benign	Het
Pafah2	GCCCC	GCCCCC	4: 134,425,541 (GRCm38)		probably null	Het
Pde1b	A	G	15: 103,525,340 (GRCm38)		probably null	Het
Pdilt	T	C	7: 119,489,384 (GRCm38)	T465A	probably benign	Het
Ppm1b	T	A	17: 84,994,124 (GRCm38)	M144K	probably benign	Het
Prkdc	T	C	16: 15,808,058 (GRCm38)	L3316S	probably benign	Het
Prm2	T	A	16: 10,791,591 (GRCm38)		probably benign	Het
Prmt7	T	C	8: 106,237,008 (GRCm38)	V240A	probably benign	Het
Prx	C	A	7: 27,518,888 (GRCm38)	A938E	possibly damaging	Het
Rbm7	A	G	9: 48,490,894 (GRCm38)	V131A	probably benign	Het
Ric1	T	A	19: 29,600,813 (GRCm38)		probably null	Het
Rnf150	A	T	8: 82,864,010 (GRCm38)	M1L	possibly damaging	Het
Rnf20	C	T	4: 49,644,628 (GRCm38)	R298W	probably damaging	Het
Rp1	T	A	1: 4,347,232 (GRCm38)	Y1219F	possibly damaging	Het
Scn7a	A	G	2: 66,684,013 (GRCm38)		probably null	Het
Sdad1	A	G	5: 92,292,651 (GRCm38)		probably null	Het
Septin9	T	C	11: 117,353,083 (GRCm38)		probably benign	Het
Serinc3	T	C	2: 163,645,489 (GRCm38)		probably benign	Het
Shc3	C	T	13: 51,461,388 (GRCm38)	G178R	probably damaging	Het
Slc4a10	A	T	2: 62,316,606 (GRCm38)	K1090M	probably damaging	Het
Slco1a1	T	C	6: 141,923,111 (GRCm38)	I376V	probably benign	Het
Slmap	A	T	14: 26,422,574 (GRCm38)	F719L	probably benign	Het
Smgc	A	G	15: 91,859,758 (GRCm38)	N573D	possibly damaging	Het
Spx	G	A	6: 142,414,079 (GRCm38)		probably null	Het
Srrt	C	G	5: 137,296,945 (GRCm38)	E308Q	probably damaging	Het
Tas2r130	T	A	6: 131,630,597 (GRCm38)	R78S	probably benign	Het
Tchhl1	A	G	3: 93,471,101 (GRCm38)	R371G	probably damaging	Het
Teddm2	C	T	1: 153,850,448 (GRCm38)	A174T	probably benign	Het
Tktl2	G	A	8: 66,512,347 (GRCm38)	V186M	probably damaging	Het
Trim33	T	C	3: 103,324,640 (GRCm38)		probably benign	Het
Tspan31	A	G	10: 127,069,458 (GRCm38)	V40A	probably damaging	Het
Uevld	A	G	7: 46,945,227 (GRCm38)		probably benign	Het
Vcl	G	T	14: 21,008,995 (GRCm38)	A560S	probably benign	Het
Vmn2r24	T	A	6: 123,816,224 (GRCm38)	C837S	probably damaging	Het
Vmn2r57	C	T	7: 41,428,107 (GRCm38)	V212M	probably damaging	Het
Vps13c	A	G	9: 67,936,340 (GRCm38)	T1968A	probably benign	Het
Vtn	A	G	11: 78,500,567 (GRCm38)	R269G	probably damaging	Het
Wdcp	G	A	12: 4,850,245 (GRCm38)	V34I	possibly damaging	Het
Zc3h14	A	G	12: 98,752,832 (GRCm38)	D152G	possibly damaging	Het
Zfp189	C	T	4: 49,529,266 (GRCm38)	P123L	probably benign	Het
Zfp318	T	C	17: 46,406,055 (GRCm38)	S1038P	possibly damaging	Het
Zfp873	A	G	10: 82,060,572 (GRCm38)	D416G	probably benign	Het

Other mutations in Plxnd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00764:Plxnd1	APN	6	115,967,972 (GRCm38)	missense	possibly damaging	0.51
IGL01099:Plxnd1	APN	6	115,969,945 (GRCm38)	missense	probably benign
IGL01323:Plxnd1	APN	6	115,966,799 (GRCm38)	missense	possibly damaging	0.81
IGL01382:Plxnd1	APN	6	115,960,527 (GRCm38)	missense	probably damaging	1.00
IGL01786:Plxnd1	APN	6	115,959,935 (GRCm38)	missense	probably damaging	1.00
IGL02244:Plxnd1	APN	6	115,978,257 (GRCm38)	missense	probably benign	0.39
IGL02272:Plxnd1	APN	6	115,993,628 (GRCm38)	missense	probably damaging	1.00
IGL02293:Plxnd1	APN	6	115,963,913 (GRCm38)	missense	probably damaging	1.00
IGL02465:Plxnd1	APN	6	115,955,742 (GRCm38)	makesense	probably null
IGL02873:Plxnd1	APN	6	115,959,976 (GRCm38)	missense	probably damaging	1.00
IGL03209:Plxnd1	APN	6	115,962,357 (GRCm38)	missense	probably damaging	1.00
Hiss	UTSW	6	115,969,929 (GRCm38)	missense	possibly damaging	0.94
murmer	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
mutter	UTSW	6	115,968,044 (GRCm38)	missense	probably benign	0.27
rattle	UTSW	6	115,959,794 (GRCm38)	missense	probably damaging	0.96
R0238:Plxnd1	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
R0238:Plxnd1	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
R0239:Plxnd1	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
R0239:Plxnd1	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
R0357:Plxnd1	UTSW	6	115,969,460 (GRCm38)	missense	probably benign	0.00
R0646:Plxnd1	UTSW	6	115,958,699 (GRCm38)	splice site	probably benign
R0648:Plxnd1	UTSW	6	115,994,001 (GRCm38)	missense	possibly damaging	0.86
R0718:Plxnd1	UTSW	6	115,966,638 (GRCm38)	missense	possibly damaging	0.68
R1116:Plxnd1	UTSW	6	115,967,005 (GRCm38)	splice site	probably null
R1292:Plxnd1	UTSW	6	115,962,683 (GRCm38)	unclassified	probably benign
R1715:Plxnd1	UTSW	6	115,968,681 (GRCm38)	missense	probably benign	0.02
R1760:Plxnd1	UTSW	6	115,967,779 (GRCm38)	missense	possibly damaging	0.95
R1799:Plxnd1	UTSW	6	115,994,057 (GRCm38)	missense	probably damaging	1.00
R1817:Plxnd1	UTSW	6	115,980,601 (GRCm38)	missense	possibly damaging	0.83
R1851:Plxnd1	UTSW	6	115,963,914 (GRCm38)	missense	probably damaging	1.00
R1864:Plxnd1	UTSW	6	115,969,441 (GRCm38)	splice site	probably null
R1865:Plxnd1	UTSW	6	115,969,441 (GRCm38)	splice site	probably null
R1875:Plxnd1	UTSW	6	115,978,084 (GRCm38)	splice site	probably null
R1899:Plxnd1	UTSW	6	115,969,363 (GRCm38)	missense	probably benign
R1913:Plxnd1	UTSW	6	115,978,017 (GRCm38)	missense	possibly damaging	0.50
R1970:Plxnd1	UTSW	6	115,962,517 (GRCm38)	missense	probably damaging	1.00
R2007:Plxnd1	UTSW	6	115,967,255 (GRCm38)	missense	probably damaging	1.00
R2134:Plxnd1	UTSW	6	115,957,548 (GRCm38)	missense	probably damaging	1.00
R2202:Plxnd1	UTSW	6	115,962,764 (GRCm38)	missense	probably benign	0.45
R2230:Plxnd1	UTSW	6	115,964,144 (GRCm38)	missense	probably damaging	1.00
R2267:Plxnd1	UTSW	6	115,962,743 (GRCm38)	missense	probably benign	0.29
R2427:Plxnd1	UTSW	6	115,967,748 (GRCm38)	critical splice donor site	probably null
R4108:Plxnd1	UTSW	6	115,959,315 (GRCm38)	missense	probably damaging	1.00
R4233:Plxnd1	UTSW	6	115,965,953 (GRCm38)	missense	probably benign	0.30
R4280:Plxnd1	UTSW	6	115,956,095 (GRCm38)	splice site	probably null
R4280:Plxnd1	UTSW	6	115,956,094 (GRCm38)	splice site	probably benign
R4346:Plxnd1	UTSW	6	115,977,980 (GRCm38)	missense	probably benign	0.16
R4439:Plxnd1	UTSW	6	115,993,976 (GRCm38)	missense	probably damaging	0.99
R4572:Plxnd1	UTSW	6	115,955,756 (GRCm38)	missense	probably damaging	1.00
R4576:Plxnd1	UTSW	6	115,968,044 (GRCm38)	missense	probably benign	0.27
R4599:Plxnd1	UTSW	6	115,994,276 (GRCm38)	missense	probably damaging	1.00
R4614:Plxnd1	UTSW	6	115,972,525 (GRCm38)	missense	possibly damaging	0.83
R4700:Plxnd1	UTSW	6	115,958,615 (GRCm38)	missense	probably damaging	1.00
R4705:Plxnd1	UTSW	6	115,958,620 (GRCm38)	missense	probably damaging	1.00
R4806:Plxnd1	UTSW	6	115,960,855 (GRCm38)	missense	probably damaging	1.00
R4944:Plxnd1	UTSW	6	115,955,765 (GRCm38)	missense	probably damaging	1.00
R4977:Plxnd1	UTSW	6	115,994,376 (GRCm38)	missense	probably damaging	1.00
R5069:Plxnd1	UTSW	6	115,965,901 (GRCm38)	missense	probably damaging	0.98
R5155:Plxnd1	UTSW	6	115,958,988 (GRCm38)	critical splice donor site	probably null
R5460:Plxnd1	UTSW	6	115,957,648 (GRCm38)	missense	probably damaging	1.00
R5729:Plxnd1	UTSW	6	115,965,877 (GRCm38)	missense	probably damaging	1.00
R5909:Plxnd1	UTSW	6	115,968,688 (GRCm38)	missense	probably benign	0.00
R5992:Plxnd1	UTSW	6	115,967,787 (GRCm38)	critical splice acceptor site	probably null
R6129:Plxnd1	UTSW	6	115,978,174 (GRCm38)	missense	probably damaging	1.00
R6254:Plxnd1	UTSW	6	115,977,960 (GRCm38)	missense	probably benign	0.01
R6273:Plxnd1	UTSW	6	115,978,492 (GRCm38)	missense	probably damaging	1.00
R6310:Plxnd1	UTSW	6	115,976,736 (GRCm38)	missense	possibly damaging	0.94
R6732:Plxnd1	UTSW	6	115,969,929 (GRCm38)	missense	possibly damaging	0.94
R6857:Plxnd1	UTSW	6	115,993,763 (GRCm38)	missense	probably benign	0.05
R7243:Plxnd1	UTSW	6	115,972,507 (GRCm38)	missense	probably benign	0.00
R7282:Plxnd1	UTSW	6	115,960,837 (GRCm38)	missense	probably damaging	1.00
R7632:Plxnd1	UTSW	6	115,976,639 (GRCm38)	missense	probably benign
R7699:Plxnd1	UTSW	6	115,959,794 (GRCm38)	missense	probably damaging	0.96
R7915:Plxnd1	UTSW	6	115,966,918 (GRCm38)	missense	probably benign	0.00
R8090:Plxnd1	UTSW	6	115,956,617 (GRCm38)	missense	probably damaging	1.00
R8382:Plxnd1	UTSW	6	115,972,472 (GRCm38)	missense	probably benign
R8507:Plxnd1	UTSW	6	115,966,905 (GRCm38)	missense	probably damaging	0.97
R8539:Plxnd1	UTSW	6	115,962,807 (GRCm38)	missense	possibly damaging	0.94
R8548:Plxnd1	UTSW	6	115,957,597 (GRCm38)	missense	probably damaging	1.00
R8963:Plxnd1	UTSW	6	115,972,545 (GRCm38)	nonsense	probably null
R9119:Plxnd1	UTSW	6	115,955,871 (GRCm38)	splice site	probably benign
R9177:Plxnd1	UTSW	6	115,966,508 (GRCm38)	missense	probably benign	0.00
R9182:Plxnd1	UTSW	6	115,993,785 (GRCm38)	missense	probably damaging	0.98
R9185:Plxnd1	UTSW	6	115,957,565 (GRCm38)	missense	probably damaging	1.00
R9226:Plxnd1	UTSW	6	115,957,563 (GRCm38)	missense	probably damaging	1.00
R9433:Plxnd1	UTSW	6	115,968,793 (GRCm38)	missense	probably benign	0.00
R9449:Plxnd1	UTSW	6	115,955,769 (GRCm38)	missense	probably damaging	1.00
R9451:Plxnd1	UTSW	6	115,963,316 (GRCm38)	missense	possibly damaging	0.72
R9599:Plxnd1	UTSW	6	115,963,313 (GRCm38)	missense	possibly damaging	0.78
R9627:Plxnd1	UTSW	6	115,963,313 (GRCm38)	missense	possibly damaging	0.78
R9644:Plxnd1	UTSW	6	115,963,313 (GRCm38)	missense	possibly damaging	0.78
R9672:Plxnd1	UTSW	6	115,963,313 (GRCm38)	missense	possibly damaging	0.78
X0024:Plxnd1	UTSW	6	115,963,310 (GRCm38)	missense	probably benign	0.02
X0026:Plxnd1	UTSW	6	115,966,784 (GRCm38)	missense	possibly damaging	0.88
Z1088:Plxnd1	UTSW	6	115,967,510 (GRCm38)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCAGCTGGCACTAACCAATG -3'
(R):5'- TCGTCATCCATGTGAGCACC -3'

Sequencing Primer
(F):5'- GCTGGCACTAACCAATGACTTG -3'
(R):5'- ATCCATGTGAGCACCCGACTG -3'

Posted On

2014-06-23