Mutagenetix > Incidental Mutation

Incidental Mutation 'R1850:Olfr741'

208025

Institutional Source

Beutler Lab

Gene Symbol

Olfr741

Ensembl Gene

ENSMUSG00000095765

Gene Name

olfactory receptor 741

Synonyms

MOR106-15, MOR106-10, GA_x6K02T2PMLR-6197851-6198786

MMRRC Submission

039874-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R1850 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50472738-50488338 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 50485598 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 47 (A47T)

Ref Sequence

ENSEMBL: ENSMUSP00000151020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071932] [ENSMUST00000205518] [ENSMUST00000213903]

AlphaFold

L7N1Y5

Predicted Effect

probably benign
Transcript: ENSMUST00000071932
AA Change: A47T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000071824
Gene: ENSMUSG00000095765
AA Change: A47T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	311	1.2e-55	PFAM
Pfam:7tm_1	45	294	2.4e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000205518
AA Change: A47T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000213903
AA Change: A47T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.8%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4430402I18Rik	T	C	19: 28,939,171		probably null	Het
6030419C18Rik	A	T	9: 58,499,109	M101L	probably benign	Het
Adam28	T	A	14: 68,639,195	Q202L	probably benign	Het
Adgb	C	A	10: 10,442,502	V199F	probably damaging	Het
Aff1	G	T	5: 103,833,907	R645S	probably damaging	Het
Aoc1	C	T	6: 48,905,268	S48F	probably benign	Het
Atf6	A	T	1: 170,819,286	N339K	probably damaging	Het
Bpifb3	C	G	2: 153,929,344	S392C	possibly damaging	Het
Camk1d	T	C	2: 5,362,015	M130V	probably benign	Het
Ces1a	T	A	8: 93,027,326	N350Y	probably damaging	Het
Cfap57	G	A	4: 118,599,894	R453C	probably damaging	Het
Chd4	T	A	6: 125,121,656	N1532K	probably damaging	Het
Chd5	T	C	4: 152,370,533	L824P	probably damaging	Het
Ckap5	G	A	2: 91,595,713	R1306H	probably damaging	Het
Crybg1	A	G	10: 43,997,674	F1146S	probably damaging	Het
Dusp12	G	A	1: 170,880,629	T173M	probably benign	Het
Dync2h1	T	C	9: 7,001,448	T3854A	probably benign	Het
Echdc1	A	T	10: 29,344,603	I252F	probably damaging	Het
Emc1	A	T	4: 139,359,373		probably benign	Het
Fbn2	C	A	18: 58,039,305		probably benign	Het
Fsip2	T	A	2: 82,984,589	N3555K	possibly damaging	Het
Gabra1	T	A	11: 42,179,576	T20S	probably benign	Het
H2afy	T	C	13: 56,096,239		probably benign	Het
Igf1	A	C	10: 87,861,374	T2P	possibly damaging	Het
Jcad	C	A	18: 4,675,730	T1164N	possibly damaging	Het
Kalrn	A	T	16: 33,975,923	S2830T	probably damaging	Het
Lepr	C	A	4: 101,733,423	A66E	possibly damaging	Het
Lmntd1	T	A	6: 145,413,480	M315L	probably benign	Het
Lrch3	A	G	16: 32,986,793	T479A	probably benign	Het
Matr3	A	G	18: 35,582,057	N237D	probably damaging	Het
Mslnl	G	A	17: 25,742,934	V128M	probably damaging	Het
Mtif2	A	G	11: 29,540,683	I462M	probably benign	Het
Nrip1	T	C	16: 76,293,344	I442V	probably damaging	Het
Olfr1082	A	T	2: 86,594,104	C241*	probably null	Het
Olfr1415	T	A	1: 92,491,402	M118L	possibly damaging	Het
Otogl	T	A	10: 107,878,064	Y498F	probably damaging	Het
Pbx3	A	T	2: 34,176,820	F351I	probably benign	Het
Pcdh18	T	C	3: 49,756,405	T154A	probably benign	Het
Pex5l	A	T	3: 32,950,876		probably null	Het
Plec	T	C	15: 76,188,232	I718V	probably benign	Het
Pparg	T	A	6: 115,450,980	Y143N	probably damaging	Het
Prl2c5	A	G	13: 13,185,792	I12V	probably benign	Het
Rcor3	T	A	1: 192,120,111	Q246L	probably benign	Het
Rnf214	A	T	9: 45,869,448		probably benign	Het
S1pr5	A	T	9: 21,244,129	S334T	probably benign	Het
Scg3	C	T	9: 75,682,167	S35N	possibly damaging	Het
Sept5	A	T	16: 18,625,210	L19Q	probably damaging	Het
Serpinb7	T	C	1: 107,428,295	F16S	probably damaging	Het
Sipa1l3	A	T	7: 29,339,126	S365R	probably damaging	Het
Slc27a1	T	C	8: 71,580,703		probably null	Het
Slc2a10	C	A	2: 165,515,213	H264Q	probably benign	Het
Slc9a3	A	G	13: 74,161,770	I526V	probably benign	Het
Smchd1	T	C	17: 71,389,771	D1203G	probably damaging	Het
Sult1b1	A	G	5: 87,520,841	W181R	probably damaging	Het
Supt6	A	G	11: 78,219,877		probably benign	Het
Tcf12	C	A	9: 71,868,215	A418S	probably damaging	Het
Tesk1	C	T	4: 43,443,576	R48C	probably damaging	Het
Tiam2	A	G	17: 3,437,235	Q677R	probably damaging	Het
Tspan8	C	T	10: 115,833,225	A55V	probably damaging	Het
Txndc11	T	C	16: 11,088,404	N421D	probably damaging	Het
Vmn1r201	A	G	13: 22,474,631	N5S	probably benign	Het
Vmn2r120	T	A	17: 57,525,826	I118L	probably benign	Het
Vps13b	A	T	15: 35,674,959		probably benign	Het
Wdfy3	A	G	5: 101,894,999	V1962A	probably damaging	Het
Zswim8	C	T	14: 20,710,747	R107*	probably null	Het

Other mutations in Olfr741

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01773:Olfr741	APN	14	50485773	missense	probably damaging	0.98
IGL01796:Olfr741	APN	14	50485541	missense	probably benign	0.28
IGL01916:Olfr741	APN	14	50485493	missense	probably benign	0.01
IGL02686:Olfr741	APN	14	50485969	missense	probably benign	0.01
IGL02874:Olfr741	APN	14	50486229	missense	possibly damaging	0.57
IGL02898:Olfr741	APN	14	50486186	missense	probably damaging	1.00
PIT4515001:Olfr741	UTSW	14	50486079	missense	probably benign	0.03
R0085:Olfr741	UTSW	14	50486334	missense	probably benign	0.16
R1777:Olfr741	UTSW	14	50486300	missense	probably benign	0.08
R2270:Olfr741	UTSW	14	50486037	missense	probably damaging	1.00
R2338:Olfr741	UTSW	14	50485640	missense	possibly damaging	0.47
R2971:Olfr741	UTSW	14	50485608	missense	probably damaging	0.99
R4594:Olfr741	UTSW	14	50486162	missense	probably benign	0.00
R5383:Olfr741	UTSW	14	50486052	nonsense	probably null
R5708:Olfr741	UTSW	14	50485995	missense	probably damaging	1.00
R6038:Olfr741	UTSW	14	50486220	missense	probably damaging	1.00
R6038:Olfr741	UTSW	14	50486220	missense	probably damaging	1.00
R7116:Olfr741	UTSW	14	50485568	missense	probably benign	0.00
R7702:Olfr741	UTSW	14	50486294	missense	possibly damaging	0.79
R8169:Olfr741	UTSW	14	50486235	missense	probably benign	0.13
R9040:Olfr741	UTSW	14	50485538	missense	probably benign	0.00
R9139:Olfr741	UTSW	14	50486250	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CAATTTCTGATGCTTTCACAGGTC -3'
(R):5'- ATCAAATGCCATGACTGCCAG -3'

Sequencing Primer
(F):5'- TGATGCTTTCACAGGTCACAGAC -3'
(R):5'- CTGCCAGGAAAAAGCATTCTG -3'

Posted On

2014-06-23