Mutagenetix > Incidental Mutation

Incidental Mutation 'R1864:Rusc2'

208467

Institutional Source

Beutler Lab

Gene Symbol

Rusc2

Ensembl Gene

ENSMUSG00000035969

Gene Name

RUN and SH3 domain containing 2

Synonyms

MMRRC Submission

039887-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.207) question?

Stock #

R1864 (G1)

Quality Score

159

Status

Not validated

Chromosome

Chromosomal Location

43381979-43427088 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 43421719 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Aspartic acid at position 713 (A713D)

Ref Sequence

ENSEMBL: ENSMUSP00000095710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035645] [ENSMUST00000098106] [ENSMUST00000131668] [ENSMUST00000149221] [ENSMUST00000173682]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000035645
AA Change: A713D

PolyPhen 2 Score 0.491 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000038379
Gene: ENSMUSG00000035969
AA Change: A713D

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC
SH3	1457	1512	7.4e-11	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098106
AA Change: A713D

PolyPhen 2 Score 0.491 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000095710
Gene: ENSMUSG00000035969
AA Change: A713D

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC
SH3	1457	1512	7.4e-11	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123447

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125393

Predicted Effect

probably benign
Transcript: ENSMUST00000131668
AA Change: A713D

PolyPhen 2 Score 0.428 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000118528
Gene: ENSMUSG00000035969
AA Change: A713D

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146710

Predicted Effect

probably benign
Transcript: ENSMUST00000149221

Predicted Effect

probably benign
Transcript: ENSMUST00000173682
AA Change: P608T

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000133715
Gene: ENSMUSG00000035969
AA Change: P608T

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	685	703	N/A	INTRINSIC
low complexity region	733	740	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149556

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150066

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154754

Coding Region Coverage

1x: 97.3%
3x: 96.7%
10x: 95.1%
20x: 92.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a RUN and SH3 domain containing protein that interacts with Rab1b and Rab1-binding protein GM130. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2012]

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg2	G	A	X: 159,265,347 (GRCm39)	M532I	probably benign	Het
Agtpbp1	A	G	13: 59,598,016 (GRCm39)	Y1198H	possibly damaging	Het
Ahcyl2	G	A	6: 29,908,354 (GRCm39)	V575M	probably damaging	Het
Akp3	TCACCACCACCACCACCACCACCACCACCAC	TCACCACCACCACCACCACCACCACCAC	1: 87,055,489 (GRCm39)		probably benign	Het
Ankrd50	A	T	3: 38,508,610 (GRCm39)	N329K	probably benign	Het
Ano4	C	A	10: 88,807,253 (GRCm39)	G741V	probably damaging	Het
Anxa1	T	C	19: 20,357,053 (GRCm39)	D191G	probably benign	Het
Apc2	C	T	10: 80,149,482 (GRCm39)	T1512I	probably damaging	Het
Aph1b	A	T	9: 66,701,395 (GRCm39)	C81S	probably benign	Het
Arhgap21	T	C	2: 20,866,015 (GRCm39)	E893G	probably damaging	Het
Arhgef28	A	T	13: 98,130,640 (GRCm39)	H399Q	probably benign	Het
Asic5	A	G	3: 81,919,294 (GRCm39)	E304G	probably benign	Het
B4galnt4	A	G	7: 140,650,446 (GRCm39)	Y771C	probably damaging	Het
Birc2	G	T	9: 7,819,518 (GRCm39)	Q465K	probably benign	Het
Btla	C	T	16: 45,070,737 (GRCm39)	T232I	probably damaging	Het
Ccl7	G	T	11: 81,937,378 (GRCm39)	K37N	probably benign	Het
Cdk17	T	C	10: 93,061,967 (GRCm39)	V233A	probably damaging	Het
Cenatac	A	G	9: 44,329,018 (GRCm39)	C66R	probably damaging	Het
Cilp2	C	A	8: 70,333,973 (GRCm39)	Q1008H	probably damaging	Het
Clcn1	T	A	6: 42,282,475 (GRCm39)	D442E	probably damaging	Het
Clcnka	T	C	4: 141,120,113 (GRCm39)	T269A	probably damaging	Het
Col12a1	A	T	9: 79,534,385 (GRCm39)		probably null	Het
Cts6	A	G	13: 61,349,393 (GRCm39)	I105T	probably benign	Het
Cyp3a25	T	C	5: 145,931,739 (GRCm39)	D123G	probably damaging	Het
D630003M21Rik	A	T	2: 158,045,105 (GRCm39)	L808Q	probably damaging	Het
Ddrgk1	A	T	2: 130,496,215 (GRCm39)	I270N	probably damaging	Het
Dhx15	T	C	5: 52,342,043 (GRCm39)	T92A	possibly damaging	Het
Dhx32	A	T	7: 133,339,025 (GRCm39)	C197S	probably benign	Het
Diaph2	G	A	X: 128,860,876 (GRCm39)	R473Q	probably damaging	Het
Dnd1	A	G	18: 36,899,057 (GRCm39)	C11R	possibly damaging	Het
Dock1	A	C	7: 134,748,236 (GRCm39)	D1566A	probably benign	Het
Drp2	A	G	X: 133,327,864 (GRCm39)	I43V	probably benign	Het
Ecm2	T	C	13: 49,683,621 (GRCm39)	V533A	probably benign	Het
Ecpas	T	A	4: 58,849,942 (GRCm39)	H427L	possibly damaging	Het
Emilin1	A	G	5: 31,075,934 (GRCm39)	E725G	probably damaging	Het
Eml2	A	G	7: 18,935,803 (GRCm39)	Y487C	probably damaging	Het
Epg5	T	A	18: 78,018,246 (GRCm39)	L919H	probably damaging	Het
Fam187a	T	A	11: 102,776,837 (GRCm39)	S214T	probably damaging	Het
Flna	T	C	X: 73,283,869 (GRCm39)	T521A	probably benign	Het
Foxi1	T	A	11: 34,157,531 (GRCm39)	I165F	probably damaging	Het
Fxr2	A	T	11: 69,543,103 (GRCm39)	K633N	probably benign	Het
Gdpd5	T	C	7: 99,098,206 (GRCm39)	I209T	probably benign	Het
Gmpr	G	T	13: 45,696,101 (GRCm39)	V278F	probably damaging	Het
Grm7	A	T	6: 111,057,384 (GRCm39)	D328V	probably benign	Het
Heatr6	T	C	11: 83,660,056 (GRCm39)	S534P	probably damaging	Het
Heph	A	G	X: 95,573,092 (GRCm39)	T792A	probably damaging	Het
Hipk2	T	A	6: 38,695,870 (GRCm39)		probably null	Het
Hps3	T	A	3: 20,074,123 (GRCm39)		probably null	Het
Hspa5	T	C	2: 34,664,553 (GRCm39)	F336L	probably damaging	Het
Insc	T	C	7: 114,441,413 (GRCm39)	I409T	probably benign	Het
Kcnj8	T	A	6: 142,515,966 (GRCm39)	H47L	probably damaging	Het
Kcnma1	T	A	14: 23,853,230 (GRCm39)	Q108L	probably damaging	Het
Klc3	A	G	7: 19,131,966 (GRCm39)	V137A	probably damaging	Het
Lcn2	T	C	2: 32,275,434 (GRCm39)	T194A	possibly damaging	Het
Lgr4	T	A	2: 109,841,742 (GRCm39)	F576I	possibly damaging	Het
Lypd6b	A	G	2: 49,837,459 (GRCm39)	I144V	possibly damaging	Het
Mctp1	G	A	13: 76,533,267 (GRCm39)	C205Y	possibly damaging	Het
Mitf	A	T	6: 97,987,383 (GRCm39)	N159I	probably damaging	Het
Morc1	T	C	16: 48,412,893 (GRCm39)	I678T	probably benign	Het
Muc4	A	G	16: 32,576,625 (GRCm39)		probably benign	Het
Myo7a	T	C	7: 97,701,463 (GRCm39)	Y2115C	probably damaging	Het
Myof	T	C	19: 37,975,153 (GRCm39)	I182V	probably benign	Het
Ncor1	A	G	11: 62,272,245 (GRCm39)	V635A	probably damaging	Het
Neb	G	T	2: 52,102,772 (GRCm39)	Y4257*	probably null	Het
Npr2	T	A	4: 43,641,258 (GRCm39)	V428E	probably benign	Het
Nufip2	A	G	11: 77,583,124 (GRCm39)	D346G	probably damaging	Het
Obsl1	G	A	1: 75,469,753 (GRCm39)	S1088F	probably benign	Het
Or10s1	T	G	9: 39,986,081 (GRCm39)	I163M	possibly damaging	Het
Or2ag1	T	C	7: 106,313,030 (GRCm39)	N286S	possibly damaging	Het
Or4c127	T	A	2: 89,832,825 (GRCm39)	V25E	probably benign	Het
Or4k38	C	T	2: 111,166,052 (GRCm39)	V124M	possibly damaging	Het
Or5ac22	A	G	16: 59,135,378 (GRCm39)	Y131H	probably damaging	Het
Or8b9	T	A	9: 37,766,560 (GRCm39)	Y149N	probably damaging	Het
Pdgfrb	G	A	18: 61,204,789 (GRCm39)	V550I	probably benign	Het
Pi4ka	A	T	16: 17,185,389 (GRCm39)	L237*	probably null	Het
Pla2r1	G	A	2: 60,259,055 (GRCm39)	T1111M	probably benign	Het
Plxnd1	A	T	6: 115,946,402 (GRCm39)		probably null	Het
Pnp	G	C	14: 51,185,430 (GRCm39)	A67P	probably benign	Het
Ppp1r3a	A	T	6: 14,718,404 (GRCm39)	S837T	probably damaging	Het
Ppp2r5e	C	G	12: 75,516,341 (GRCm39)	A239P	probably damaging	Het
Prom2	T	C	2: 127,381,707 (GRCm39)	D203G	probably benign	Het
Pum1	T	C	4: 130,478,836 (GRCm39)	V486A	possibly damaging	Het
Rnf115	A	G	3: 96,635,153 (GRCm39)		probably benign	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Ryr3	A	T	2: 112,560,673 (GRCm39)	H3009Q	possibly damaging	Het
Serpina1d	C	A	12: 103,734,256 (GRCm39)	C16F	probably benign	Het
Serpinf2	G	T	11: 75,328,309 (GRCm39)	R80S	possibly damaging	Het
Sh2d4a	C	A	8: 68,781,967 (GRCm39)	Q192K	probably benign	Het
Sh3d21	T	A	4: 126,044,729 (GRCm39)		probably null	Het
Sh3rf2	T	A	18: 42,187,046 (GRCm39)	L55Q	probably damaging	Het
Shc4	T	C	2: 125,481,287 (GRCm39)	D255G	probably damaging	Het
Skint2	T	A	4: 112,483,106 (GRCm39)	H170Q	probably benign	Het
Slc29a4	A	G	5: 142,703,509 (GRCm39)	Y261C	probably damaging	Het
Slc35a5	A	T	16: 44,964,071 (GRCm39)	N102K	possibly damaging	Het
Slc38a3	A	T	9: 107,533,152 (GRCm39)	I307K	probably damaging	Het
Sv2b	A	T	7: 74,773,828 (GRCm39)	S548T	probably benign	Het
Tgfbi	T	C	13: 56,780,694 (GRCm39)	S524P	probably benign	Het
Tgm5	T	C	2: 120,905,699 (GRCm39)	D152G	probably damaging	Het
Tmem213	A	G	6: 38,086,487 (GRCm39)	T48A	possibly damaging	Het
Tmem37	A	G	1: 119,995,952 (GRCm39)	S42P	probably damaging	Het
Trpm1	A	G	7: 63,917,764 (GRCm39)	K1258R	probably damaging	Het
Ttc22	T	C	4: 106,494,003 (GRCm39)	V321A	probably benign	Het
Ube2c	C	A	2: 164,611,943 (GRCm39)	A15E	probably benign	Het
Ubn2	T	A	6: 38,417,425 (GRCm39)	D154E	possibly damaging	Het
Umod	A	T	7: 119,062,478 (GRCm39)	L631M	probably damaging	Het
Ush1c	G	A	7: 45,868,816 (GRCm39)	Q373*	probably null	Het
Vmn1r238	T	A	18: 3,123,040 (GRCm39)	R125*	probably null	Het
Wnt8b	T	C	19: 44,482,029 (GRCm39)	L14P	probably benign	Het
Wrn	G	A	8: 33,778,892 (GRCm39)	A563V	probably damaging	Het
Zfp268	A	T	4: 145,348,998 (GRCm39)	Q145L	possibly damaging	Het
Zfp608	A	G	18: 55,030,983 (GRCm39)	S986P	probably benign	Het
Znrf3	T	C	11: 5,233,373 (GRCm39)	H228R	possibly damaging	Het

Other mutations in Rusc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01358:Rusc2	APN	4	43,426,116 (GRCm39)	missense	probably damaging	0.97
IGL01474:Rusc2	APN	4	43,416,434 (GRCm39)	missense	probably damaging	0.98
IGL01541:Rusc2	APN	4	43,415,840 (GRCm39)	missense	probably benign	0.08
IGL01628:Rusc2	APN	4	43,425,729 (GRCm39)	missense	probably damaging	1.00
IGL01969:Rusc2	APN	4	43,415,738 (GRCm39)	missense	probably benign	0.02
IGL02030:Rusc2	APN	4	43,416,095 (GRCm39)	missense	possibly damaging	0.86
IGL02079:Rusc2	APN	4	43,425,668 (GRCm39)	missense	probably benign
IGL02115:Rusc2	APN	4	43,426,136 (GRCm39)	splice site	probably benign
IGL02122:Rusc2	APN	4	43,421,685 (GRCm39)	missense	possibly damaging	0.67
IGL02350:Rusc2	APN	4	43,425,351 (GRCm39)	missense	possibly damaging	0.86
IGL02357:Rusc2	APN	4	43,425,351 (GRCm39)	missense	possibly damaging	0.86
IGL02437:Rusc2	APN	4	43,415,545 (GRCm39)	missense	probably damaging	1.00
IGL02930:Rusc2	APN	4	43,416,376 (GRCm39)	missense	probably damaging	0.99
IGL03154:Rusc2	APN	4	43,425,806 (GRCm39)	missense	probably benign	0.00
P0026:Rusc2	UTSW	4	43,415,840 (GRCm39)	missense	possibly damaging	0.93
R0036:Rusc2	UTSW	4	43,424,009 (GRCm39)	missense	probably damaging	1.00
R0068:Rusc2	UTSW	4	43,424,100 (GRCm39)	splice site	probably benign
R0068:Rusc2	UTSW	4	43,424,100 (GRCm39)	splice site	probably benign
R0114:Rusc2	UTSW	4	43,422,055 (GRCm39)	missense	probably damaging	1.00
R0255:Rusc2	UTSW	4	43,423,954 (GRCm39)	missense	probably damaging	1.00
R0471:Rusc2	UTSW	4	43,425,486 (GRCm39)	missense	probably damaging	0.99
R1381:Rusc2	UTSW	4	43,416,137 (GRCm39)	missense	probably damaging	1.00
R1413:Rusc2	UTSW	4	43,416,568 (GRCm39)	missense	probably benign	0.00
R1416:Rusc2	UTSW	4	43,421,617 (GRCm39)	missense	possibly damaging	0.86
R1731:Rusc2	UTSW	4	43,426,046 (GRCm39)	missense	probably benign
R1897:Rusc2	UTSW	4	43,421,749 (GRCm39)	missense	probably damaging	1.00
R2010:Rusc2	UTSW	4	43,415,212 (GRCm39)	missense	probably benign	0.06
R2212:Rusc2	UTSW	4	43,415,935 (GRCm39)	missense	probably damaging	1.00
R2275:Rusc2	UTSW	4	43,416,260 (GRCm39)	missense	probably damaging	1.00
R2885:Rusc2	UTSW	4	43,415,456 (GRCm39)	missense	probably benign	0.28
R2886:Rusc2	UTSW	4	43,415,456 (GRCm39)	missense	probably benign	0.28
R3412:Rusc2	UTSW	4	43,415,935 (GRCm39)	missense	probably damaging	1.00
R3413:Rusc2	UTSW	4	43,415,935 (GRCm39)	missense	probably damaging	1.00
R3414:Rusc2	UTSW	4	43,415,935 (GRCm39)	missense	probably damaging	1.00
R3852:Rusc2	UTSW	4	43,416,424 (GRCm39)	missense	probably benign	0.45
R4135:Rusc2	UTSW	4	43,425,563 (GRCm39)	missense	possibly damaging	0.49
R4272:Rusc2	UTSW	4	43,415,533 (GRCm39)	missense	probably damaging	1.00
R4574:Rusc2	UTSW	4	43,416,080 (GRCm39)	missense	probably damaging	0.99
R4888:Rusc2	UTSW	4	43,423,942 (GRCm39)	missense	probably damaging	1.00
R5010:Rusc2	UTSW	4	43,415,926 (GRCm39)	missense	probably damaging	1.00
R5071:Rusc2	UTSW	4	43,415,240 (GRCm39)	missense	probably benign	0.05
R5131:Rusc2	UTSW	4	43,414,948 (GRCm39)	missense	probably benign	0.03
R5177:Rusc2	UTSW	4	43,421,805 (GRCm39)	splice site	probably null
R5540:Rusc2	UTSW	4	43,423,975 (GRCm39)	missense	probably damaging	1.00
R5561:Rusc2	UTSW	4	43,415,932 (GRCm39)	nonsense	probably null
R5628:Rusc2	UTSW	4	43,425,348 (GRCm39)	missense	probably damaging	1.00
R5645:Rusc2	UTSW	4	43,425,758 (GRCm39)	missense	probably benign	0.06
R6129:Rusc2	UTSW	4	43,424,271 (GRCm39)	missense	probably damaging	1.00
R6362:Rusc2	UTSW	4	43,416,416 (GRCm39)	missense	probably benign	0.30
R6633:Rusc2	UTSW	4	43,414,852 (GRCm39)	missense	probably damaging	0.99
R6980:Rusc2	UTSW	4	43,422,846 (GRCm39)	missense	probably benign	0.35
R7491:Rusc2	UTSW	4	43,426,528 (GRCm39)	missense	probably damaging	1.00
R7641:Rusc2	UTSW	4	43,425,335 (GRCm39)	missense	possibly damaging	0.84
R7698:Rusc2	UTSW	4	43,414,900 (GRCm39)	nonsense	probably null
R7710:Rusc2	UTSW	4	43,416,119 (GRCm39)	missense	probably benign	0.07
R8052:Rusc2	UTSW	4	43,421,851 (GRCm39)	missense	probably benign
R8061:Rusc2	UTSW	4	43,422,492 (GRCm39)	missense	probably damaging	1.00
R8127:Rusc2	UTSW	4	43,423,747 (GRCm39)	missense	possibly damaging	0.54
R8319:Rusc2	UTSW	4	43,425,378 (GRCm39)	missense	probably damaging	1.00
R8355:Rusc2	UTSW	4	43,422,846 (GRCm39)	missense	probably benign	0.35
R8397:Rusc2	UTSW	4	43,424,206 (GRCm39)	missense	possibly damaging	0.95
R8455:Rusc2	UTSW	4	43,422,846 (GRCm39)	missense	probably benign	0.35
R8553:Rusc2	UTSW	4	43,416,508 (GRCm39)	missense	probably benign	0.05
R8725:Rusc2	UTSW	4	43,401,351 (GRCm39)	intron	probably benign
R8725:Rusc2	UTSW	4	43,415,396 (GRCm39)	missense	probably damaging	0.99
R8727:Rusc2	UTSW	4	43,401,351 (GRCm39)	intron	probably benign
R8834:Rusc2	UTSW	4	43,416,431 (GRCm39)	missense	possibly damaging	0.94
R9295:Rusc2	UTSW	4	43,416,382 (GRCm39)	missense	probably damaging	0.98
R9483:Rusc2	UTSW	4	43,415,897 (GRCm39)	missense	probably damaging	0.97
R9666:Rusc2	UTSW	4	43,416,262 (GRCm39)	missense	probably benign	0.21
R9705:Rusc2	UTSW	4	43,424,936 (GRCm39)	missense	probably benign	0.00
X0025:Rusc2	UTSW	4	43,422,226 (GRCm39)	missense	probably benign	0.00
X0066:Rusc2	UTSW	4	43,422,204 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATTGGGTATCACCGATCCTTGTG -3'
(R):5'- AGTCGGTGCTAGACCCAAAG -3'

Sequencing Primer
(F):5'- GGTATCACCGATCCTTGTGATTCTTC -3'
(R):5'- AGGTCTCTGGTTCAGGCC -3'

Posted On

2014-06-30