Mutagenetix > Incidental Mutation

Incidental Mutation 'R1884:Coro7'

209392

Institutional Source

Beutler Lab

Gene Symbol

Coro7

Ensembl Gene

ENSMUSG00000039637

Gene Name

coronin 7

Synonyms

0610011B16Rik

MMRRC Submission

039905-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R1884 (G1)

Quality Score

223

Status

Validated

Chromosome

Chromosomal Location

4626133-4679777 bp(-) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to T at 4628819 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000119723 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000014445] [ENSMUST00000038552] [ENSMUST00000135823] [ENSMUST00000156889]

AlphaFold

Q9D2V7

Predicted Effect

probably benign
Transcript: ENSMUST00000014445

SMART Domains

Protein: ENSMUSP00000014445
Gene: ENSMUSG00000014301

Domain	Start	End	E-Value	Type
Pfam:Pam16	1	125	4.9e-63	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000038552

SMART Domains

Protein: ENSMUSP00000048489
Gene: ENSMUSG00000039637

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART
DUF1900	251	385	4.49e-60	SMART
low complexity region	427	456	N/A	INTRINSIC
DUF1899	463	528	1.2e-19	SMART
WD40	531	570	3.64e-2	SMART
WD40	580	620	8.55e-8	SMART
WD40	623	662	1.16e-9	SMART
low complexity region	667	679	N/A	INTRINSIC
DUF1900	718	854	6.69e-68	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127120

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127125

Predicted Effect

probably benign
Transcript: ENSMUST00000135823

SMART Domains

Protein: ENSMUSP00000118310
Gene: ENSMUSG00000039637

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139072

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143723

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150028

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150724

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151156

Predicted Effect

probably benign
Transcript: ENSMUST00000156889

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000229767

Coding Region Coverage

1x: 97.3%
3x: 96.7%
10x: 95.1%
20x: 92.3%

Validation Efficiency

99% (94/95)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele are viable and overtly normal but exhibit disruption of the Golgi apparatus. Mutant fibroblasts show increased cell spreading and cellular F-actin content, increased cell polarization and migration, and enhanced wound healing in a scratch-wound assay. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad11	T	C	9: 104,114,485	M573T	probably benign	Het
Ank3	A	G	10: 70,015,592	T1162A	possibly damaging	Het
Anxa2	TCCC	TCC	9: 69,489,754		probably null	Het
Arpp21	T	A	9: 112,143,527	D232V	probably damaging	Het
Atg2a	T	C	19: 6,254,384	Y1144H	probably damaging	Het
Auh	G	A	13: 52,835,496	P308L	probably benign	Het
Brs3	T	C	X: 57,047,089	I311T	probably benign	Het
Cenpf	C	T	1: 189,646,849	V2915I	probably benign	Het
Cep170	G	T	1: 176,774,679	T287K	probably benign	Het
Chst2	G	A	9: 95,405,558	T245I	probably damaging	Het
Cnot4	C	T	6: 35,078,157	V66I	probably damaging	Het
Cntn4	A	G	6: 106,679,392	T885A	probably benign	Het
Col20a1	C	T	2: 180,992,910	T131I	possibly damaging	Het
Cpne8	C	A	15: 90,648,628		probably benign	Het
Dbnl	G	A	11: 5,799,247	G356E	probably benign	Het
Ddx31	T	A	2: 28,858,990	I266N	probably damaging	Het
Dnah7a	A	T	1: 53,541,000	I1592N	probably damaging	Het
Dok6	A	T	18: 89,474,006	L149Q	probably damaging	Het
Eri2	G	A	7: 119,791,123	T94I	probably benign	Het
Ermp1	A	G	19: 29,616,679	V697A	probably benign	Het
Foxc1	C	T	13: 31,807,665	T153M	probably damaging	Het
Fry	A	G	5: 150,403,520	I1224V	probably benign	Het
Fzd5	G	A	1: 64,735,654	T316M	probably damaging	Het
Gcm1	G	T	9: 78,059,579	V27L	probably benign	Het
Gm5773	A	T	3: 93,774,041	D340V	probably benign	Het
Gucy2d	T	A	7: 98,451,608	H379Q	probably benign	Het
Hectd1	T	A	12: 51,800,955	M392L	probably benign	Het
Itpr2	A	C	6: 146,385,971	D452E	probably benign	Het
Krtap2-4	G	C	11: 99,614,679		probably benign	Het
Lrit1	T	C	14: 37,061,753	V346A	possibly damaging	Het
Lrrk1	A	G	7: 66,262,437	S1792P	probably benign	Het
Marveld2	A	T	13: 100,600,621	V168E	probably benign	Het
Ms4a4b	T	A	19: 11,461,265	D149E	probably damaging	Het
Myom2	G	A	8: 15,114,278	D1058N	probably benign	Het
Ncam2	C	T	16: 81,437,683	P142L	probably damaging	Het
Nek9	A	G	12: 85,332,556	L192P	probably damaging	Het
Nol10	T	C	12: 17,368,389		probably null	Het
Nrcam	T	C	12: 44,544,755	V194A	probably damaging	Het
Nudt6	A	G	3: 37,412,400	V82A	probably benign	Het
Odf2l	A	T	3: 145,151,048	N492I	probably damaging	Het
Olfr446	T	C	6: 42,927,830	S200P	probably damaging	Het
Olfr583	T	C	7: 103,051,982	I228T	probably benign	Het
Pkn3	T	C	2: 30,082,828	V276A	probably damaging	Het
Prl3b1	T	G	13: 27,247,903	L137R	possibly damaging	Het
Rab11fip2	A	G	19: 59,937,330	F10L	probably damaging	Het
Rab3b	T	A	4: 108,929,452	V133E	probably damaging	Het
Rnf111	A	T	9: 70,476,238	S138T	probably damaging	Het
Rsf1	G	A	7: 97,579,910		probably benign	Het
Runx1t1	A	G	4: 13,835,767	T75A	probably benign	Het
Ryr2	T	G	13: 11,738,356	K1693T	probably damaging	Het
Sdha	A	T	13: 74,333,136	I317N	probably damaging	Het
Serpina1d	T	A	12: 103,765,778	D274V	possibly damaging	Het
Setd2	T	G	9: 110,556,418		probably null	Het
Sh3tc2	A	G	18: 62,008,575	Y1109C	probably damaging	Het
Sik3	C	G	9: 46,221,089	H1276Q	probably benign	Het
Slc22a2	A	G	17: 12,614,826		probably benign	Het
Slc35f4	A	T	14: 49,313,634	W108R	probably damaging	Het
Slc4a1ap	A	G	5: 31,534,180	E440G	probably damaging	Het
Slc6a4	A	G	11: 77,013,375	T219A	probably benign	Het
Stxbp5	C	A	10: 9,812,298	V420F	possibly damaging	Het
Svil	T	G	18: 5,094,640	V1440G	possibly damaging	Het
Tas2r126	A	T	6: 42,435,027	T165S	probably benign	Het
Tbck	T	C	3: 132,724,916		probably null	Het
Tnxb	T	A	17: 34,689,565	D1520E	probably benign	Het
Top1mt	C	T	15: 75,667,901	R287H	possibly damaging	Het
Trdn	A	G	10: 33,257,095	K314E	probably benign	Het
Troap	T	A	15: 99,077,898	C233S	probably benign	Het
Usp28	T	C	9: 49,035,947	M571T	probably damaging	Het
Vac14	A	G	8: 110,711,687	H644R	probably damaging	Het
Vipr1	C	T	9: 121,665,864	P327L	possibly damaging	Het
Vmn1r31	C	T	6: 58,472,044	V279I	probably damaging	Het
Vmn1r69	G	A	7: 10,580,751	R18W	probably benign	Het
Vmn2r18	G	T	5: 151,575,725	Q425K	probably benign	Het
Vmn2r60	T	C	7: 42,136,670	V299A	probably damaging	Het
Vps13b	A	T	15: 35,430,291		probably benign	Het
Zfp157	A	G	5: 138,444,840	D31G	probably damaging	Het
Zfp385c	A	C	11: 100,630,706	V176G	probably benign	Het
Zfp831	A	G	2: 174,704,077	H1325R	possibly damaging	Het

Other mutations in Coro7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Coro7	APN	16	4634636	missense	possibly damaging	0.83
IGL00885:Coro7	APN	16	4635026	missense	probably benign	0.00
IGL02944:Coro7	APN	16	4635412	missense	probably benign	0.14
IGL03104:Coro7	APN	16	4629126	missense	probably damaging	1.00
IGL03153:Coro7	APN	16	4635382	critical splice donor site	probably null
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0080:Coro7	UTSW	16	4630464	missense	probably damaging	1.00
R0193:Coro7	UTSW	16	4627504	unclassified	probably benign
R0242:Coro7	UTSW	16	4630178	splice site	probably benign
R0318:Coro7	UTSW	16	4675807	missense	probably benign	0.09
R0554:Coro7	UTSW	16	4632257	missense	possibly damaging	0.63
R0666:Coro7	UTSW	16	4631911	missense	possibly damaging	0.70
R0835:Coro7	UTSW	16	4632254	missense	probably benign	0.12
R0968:Coro7	UTSW	16	4670055	splice site	probably benign
R1670:Coro7	UTSW	16	4628233	missense	possibly damaging	0.76
R1709:Coro7	UTSW	16	4634441	splice site	probably null
R1848:Coro7	UTSW	16	4630434	missense	probably damaging	0.99
R1935:Coro7	UTSW	16	4628732	missense	probably benign
R1937:Coro7	UTSW	16	4628732	missense	probably benign
R1939:Coro7	UTSW	16	4628732	missense	probably benign
R1967:Coro7	UTSW	16	4634889	missense	probably damaging	1.00
R1969:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R1970:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R3034:Coro7	UTSW	16	4632291	missense	probably damaging	0.99
R4638:Coro7	UTSW	16	4632287	missense	probably damaging	0.96
R4710:Coro7	UTSW	16	4634933	intron	probably benign
R4723:Coro7	UTSW	16	4631994	missense	probably benign	0.00
R4789:Coro7	UTSW	16	4628221	missense	probably damaging	1.00
R5493:Coro7	UTSW	16	4632487	missense	probably damaging	0.99
R5619:Coro7	UTSW	16	4676935	critical splice donor site	probably null
R5756:Coro7	UTSW	16	4632284	missense	probably damaging	0.97
R5974:Coro7	UTSW	16	4631889	missense	possibly damaging	0.83
R6010:Coro7	UTSW	16	4669956	missense	possibly damaging	0.68
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6906:Coro7	UTSW	16	4633304	missense	probably benign	0.00
R6925:Coro7	UTSW	16	4628674	critical splice donor site	probably null
R7069:Coro7	UTSW	16	4679611	start codon destroyed	probably damaging	0.99
R7326:Coro7	UTSW	16	4632048	missense	probably damaging	0.96
R7421:Coro7	UTSW	16	4668751	missense	probably benign	0.19
R7521:Coro7	UTSW	16	4631482	missense	probably benign	0.00
R7773:Coro7	UTSW	16	4632006	missense	probably damaging	1.00
R7846:Coro7	UTSW	16	4670536	missense	probably damaging	1.00
R8240:Coro7	UTSW	16	4668796	missense	probably damaging	0.96
R8726:Coro7	UTSW	16	4668755	missense	possibly damaging	0.95
R8762:Coro7	UTSW	16	4634339	missense	probably benign
R9383:Coro7	UTSW	16	4635024	missense	probably damaging	1.00
R9451:Coro7	UTSW	16	4670538	missense	probably damaging	1.00
R9553:Coro7	UTSW	16	4668760	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- ACGAAGGACTGTACTTGGGC -3'
(R):5'- ACCTGTTCTTGCACCCAAAC -3'

Sequencing Primer
(F):5'- GACTGTACTTGGGCCTCCTG -3'
(R):5'- AAACCTCCCAGCTGTCTACTTTGG -3'

Posted On

2014-06-30