Mutagenetix > Incidental Mutation

Incidental Mutation 'R0118:Polr3g'

20947

Institutional Source

Beutler Lab

Gene Symbol

Polr3g

Ensembl Gene

ENSMUSG00000035834

Gene Name

polymerase (RNA) III (DNA directed) polypeptide G

Synonyms

RPC32, 2310047G20Rik

MMRRC Submission

038404-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0118 (G1)

Quality Score

214

Status

Validated (trace)

Chromosome

Chromosomal Location

81821962-81859132 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 81824240 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152940 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048993] [ENSMUST00000049055] [ENSMUST00000224300]

AlphaFold

Q6NXY9

Predicted Effect

unknown
Transcript: ENSMUST00000048993
AA Change: E196G

SMART Domains

Protein: ENSMUSP00000035289
Gene: ENSMUSG00000035834
AA Change: E196G

Domain	Start	End	E-Value	Type
Pfam:RNA_pol_3_Rpc31	1	223	7e-41	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000049055

SMART Domains

Protein: ENSMUSP00000038275
Gene: ENSMUSG00000035840

Domain	Start	End	E-Value	Type
LysM	66	110	4.6e-4	SMART
low complexity region	149	158	N/A	INTRINSIC
transmembrane domain	214	236	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162535

Predicted Effect

probably benign
Transcript: ENSMUST00000224300

Meta Mutation Damage Score

0.1288

Coding Region Coverage

1x: 98.8%
3x: 97.2%
10x: 89.3%
20x: 67.4%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts15	T	C	9: 30,823,040 (GRCm39)	R343G	probably damaging	Het
Asxl2	T	G	12: 3,546,923 (GRCm39)	V569G	probably damaging	Het
Azin2	A	C	4: 128,843,430 (GRCm39)	H85Q	probably damaging	Het
Cacna1a	C	T	8: 85,262,712 (GRCm39)	R324C	probably damaging	Het
Ccr3	C	A	9: 123,829,647 (GRCm39)	Y327*	probably null	Het
Cers2	T	C	3: 95,227,537 (GRCm39)	F55S	probably benign	Het
Cic	C	T	7: 24,985,459 (GRCm39)	S301L	probably damaging	Het
Cntnap2	T	C	6: 45,037,326 (GRCm39)		probably null	Het
Cpn2	T	C	16: 30,079,186 (GRCm39)	R172G	probably benign	Het
Ctdnep1	T	C	11: 69,879,557 (GRCm39)		probably null	Het
Dennd3	T	A	15: 73,436,925 (GRCm39)	Y1051N	probably damaging	Het
Dmap1	T	G	4: 117,533,680 (GRCm39)	Y196S	probably damaging	Het
Entpd7	G	A	19: 43,692,751 (GRCm39)	W102*	probably null	Het
Frem2	A	T	3: 53,442,664 (GRCm39)	C2624*	probably null	Het
Gdpd3	A	G	7: 126,370,165 (GRCm39)	Y238C	probably damaging	Het
Gjb3	A	G	4: 127,220,451 (GRCm39)	V27A	probably damaging	Het
Kat6b	T	C	14: 21,720,042 (GRCm39)	F1465L	probably damaging	Het
Klra17	A	T	6: 129,808,552 (GRCm39)	M227K	probably benign	Het
Map6	A	G	7: 98,966,824 (GRCm39)	D348G	possibly damaging	Het
Mapkbp1	T	C	2: 119,855,696 (GRCm39)	S1472P	probably benign	Het
Megf6	C	A	4: 154,339,098 (GRCm39)	P545Q	probably damaging	Het
Mertk	C	T	2: 128,601,086 (GRCm39)	R357W	probably damaging	Het
Mesd	T	A	7: 83,544,835 (GRCm39)	I104N	probably damaging	Het
Mrm3	T	A	11: 76,140,781 (GRCm39)	V263E	possibly damaging	Het
Ndst4	T	A	3: 125,405,210 (GRCm39)	Y488*	probably null	Het
Nfat5	C	T	8: 108,065,707 (GRCm39)	R156W	probably damaging	Het
Nfs1	T	C	2: 155,976,444 (GRCm39)	H150R	probably damaging	Het
Odad3	A	T	9: 21,906,353 (GRCm39)	N224K	probably benign	Het
Or1n1b	A	G	2: 36,780,035 (GRCm39)	M275T	probably benign	Het
Or8b56	T	C	9: 38,739,154 (GRCm39)	S50P	possibly damaging	Het
Or8g19	T	A	9: 39,055,399 (GRCm39)	M1K	probably null	Het
Or9q1	A	G	19: 13,804,929 (GRCm39)	F277S	possibly damaging	Het
Pcdh8	T	C	14: 80,004,848 (GRCm39)	Y1059C	probably damaging	Het
Pik3r5	T	A	11: 68,381,306 (GRCm39)	L164Q	probably damaging	Het
Ppm1e	T	A	11: 87,122,564 (GRCm39)	K464N	probably benign	Het
Rims1	T	C	1: 22,416,631 (GRCm39)	T1037A	probably damaging	Het
Rpgrip1l	A	T	8: 91,996,750 (GRCm39)	I108N	probably damaging	Het
Sfi1	CCTCTC	CCTCTCTC	11: 3,127,419 (GRCm39)		probably benign	Het
Spem1	T	C	11: 69,712,371 (GRCm39)	K98E	possibly damaging	Het
St7l	T	C	3: 104,796,619 (GRCm39)	V237A	probably damaging	Het
Tbc1d16	T	C	11: 119,048,642 (GRCm39)	H337R	probably damaging	Het
Tbc1d32	T	A	10: 55,893,701 (GRCm39)	I1291F	probably benign	Het
Tnfaip6	G	T	2: 51,933,827 (GRCm39)	E61*	probably null	Het
Trib2	A	T	12: 15,843,929 (GRCm39)	W102R	probably damaging	Het
Uimc1	G	T	13: 55,233,457 (GRCm39)	N66K	probably damaging	Het
Vmn1r63	T	A	7: 5,805,838 (GRCm39)	T265S	probably benign	Het
Vps35	G	A	8: 86,021,582 (GRCm39)	T3I	probably benign	Het
Yeats2	T	A	16: 19,975,692 (GRCm39)	L63*	probably null	Het
Zfp282	A	G	6: 47,869,866 (GRCm39)	R304G	probably benign	Het

Other mutations in Polr3g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Polr3g	APN	13	81,842,796 (GRCm39)	missense	probably damaging	1.00
IGL01764:Polr3g	APN	13	81,830,238 (GRCm39)	missense	possibly damaging	0.93
R0550:Polr3g	UTSW	13	81,842,892 (GRCm39)	missense	probably damaging	0.99
R3725:Polr3g	UTSW	13	81,842,754 (GRCm39)	missense	probably damaging	0.99
R6536:Polr3g	UTSW	13	81,826,335 (GRCm39)	missense	unknown
R6684:Polr3g	UTSW	13	81,847,650 (GRCm39)	splice site	probably null
R7645:Polr3g	UTSW	13	81,842,563 (GRCm39)	missense	unknown
R8296:Polr3g	UTSW	13	81,842,682 (GRCm39)	missense	unknown
R8317:Polr3g	UTSW	13	81,826,302 (GRCm39)	missense	unknown
R9178:Polr3g	UTSW	13	81,842,535 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GACGAGTAACCCTTGCTACCGAAG -3'
(R):5'- GCTGCACCTGAGCACAGTAGAAAAG -3'

Sequencing Primer
(F):5'- CGCATTGCATAGTCACAGGTG -3'
(R):5'- GCTGGGCTGATCAAAATGTG -3'

Posted On

2013-04-11