Incidental Mutation 'R1911:Robo2'
| ID |
210313 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Robo2
|
| Ensembl Gene |
ENSMUSG00000052516 |
| Gene Name |
roundabout guidance receptor 2 |
| Synonyms |
2600013A04Rik, 9430089E08Rik, D230004I22Rik |
| MMRRC Submission |
039929-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.951)
|
| Stock # |
R1911 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
16 |
| Chromosomal Location |
73688727-74208713 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 73755213 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Asparagine to Lysine
at position 769
(N769K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000113795
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000117200]
[ENSMUST00000117785]
[ENSMUST00000226478]
[ENSMUST00000227347]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000116586
AA Change: N773K
PolyPhen 2
Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000112285
Gene: ENSMUSG00000052516
AA Change: N773K
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
|
IGc2
|
43 |
117 |
3.56e-9 |
SMART |
|
IGc2
|
145 |
210 |
3.33e-9 |
SMART |
|
IGc2
|
237 |
300 |
6.59e-13 |
SMART |
|
IGc2
|
330 |
402 |
1.3e-11 |
SMART |
|
IGc2
|
434 |
499 |
3.73e-12 |
SMART |
|
FN3
|
526 |
608 |
1.42e-15 |
SMART |
|
FN3
|
640 |
725 |
3.54e-2 |
SMART |
|
FN3
|
740 |
827 |
6.15e-11 |
SMART |
|
transmembrane domain
|
864 |
886 |
N/A |
INTRINSIC |
|
low complexity region
|
1044 |
1069 |
N/A |
INTRINSIC |
|
low complexity region
|
1076 |
1087 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000117200
AA Change: N769K
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000113795
Gene: ENSMUSG00000052516
AA Change: N769K
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
|
IGc2
|
43 |
117 |
3.56e-9 |
SMART |
|
IGc2
|
145 |
210 |
3.33e-9 |
SMART |
|
IGc2
|
237 |
300 |
6.59e-13 |
SMART |
|
IGc2
|
326 |
398 |
1.3e-11 |
SMART |
|
IGc2
|
430 |
495 |
3.73e-12 |
SMART |
|
FN3
|
522 |
604 |
1.42e-15 |
SMART |
|
FN3
|
636 |
721 |
3.54e-2 |
SMART |
|
FN3
|
736 |
823 |
6.15e-11 |
SMART |
|
transmembrane domain
|
860 |
882 |
N/A |
INTRINSIC |
|
low complexity region
|
1040 |
1065 |
N/A |
INTRINSIC |
|
low complexity region
|
1072 |
1083 |
N/A |
INTRINSIC |
|
low complexity region
|
1191 |
1199 |
N/A |
INTRINSIC |
|
low complexity region
|
1210 |
1234 |
N/A |
INTRINSIC |
|
low complexity region
|
1318 |
1342 |
N/A |
INTRINSIC |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000117785
AA Change: N769K
PolyPhen 2
Score 0.930 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000112776
Gene: ENSMUSG00000052516
AA Change: N769K
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
|
IGc2
|
43 |
117 |
3.56e-9 |
SMART |
|
IGc2
|
145 |
210 |
3.33e-9 |
SMART |
|
IGc2
|
237 |
300 |
6.59e-13 |
SMART |
|
IGc2
|
326 |
398 |
1.3e-11 |
SMART |
|
IGc2
|
430 |
495 |
3.73e-12 |
SMART |
|
FN3
|
522 |
604 |
1.42e-15 |
SMART |
|
FN3
|
636 |
721 |
3.54e-2 |
SMART |
|
FN3
|
736 |
823 |
6.15e-11 |
SMART |
|
transmembrane domain
|
860 |
882 |
N/A |
INTRINSIC |
|
low complexity region
|
1072 |
1107 |
N/A |
INTRINSIC |
|
low complexity region
|
1114 |
1125 |
N/A |
INTRINSIC |
|
low complexity region
|
1233 |
1241 |
N/A |
INTRINSIC |
|
low complexity region
|
1252 |
1276 |
N/A |
INTRINSIC |
|
low complexity region
|
1351 |
1362 |
N/A |
INTRINSIC |
|
low complexity region
|
1451 |
1475 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000226478
AA Change: N773K
PolyPhen 2
Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000227347
AA Change: N773K
PolyPhen 2
Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000231889
AA Change: N95K
|
| Meta Mutation Damage Score |
0.2725 |
| Coding Region Coverage |
- 1x: 97.3%
- 3x: 96.8%
- 10x: 95.4%
- 20x: 93.2%
|
| Validation Efficiency |
99% (99/100) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygous mutants display postnatal lethality, abnormal ureteric bud development, multiple fused kidneys, multiple ureters, and abnormal commissural axon growth. [provided by MGI curators]
|
| Allele List at MGI |
All alleles(6) : Targeted(3) Gene trapped(3)
|
| Other mutations in this stock |
Total: 96 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930590J08Rik |
T |
A |
6: 91,927,050 (GRCm39) |
|
probably benign |
Het |
| Abca7 |
T |
C |
10: 79,842,468 (GRCm39) |
V1134A |
probably benign |
Het |
| Acaa2 |
A |
G |
18: 74,925,483 (GRCm39) |
E82G |
probably benign |
Het |
| Acap1 |
T |
C |
11: 69,772,548 (GRCm39) |
D521G |
probably damaging |
Het |
| Adam19 |
T |
C |
11: 46,012,281 (GRCm39) |
V259A |
probably damaging |
Het |
| Adss1 |
T |
C |
12: 112,599,443 (GRCm39) |
V140A |
probably benign |
Het |
| Aif1 |
G |
A |
17: 35,391,127 (GRCm39) |
P44L |
probably benign |
Het |
| Aldh3b1 |
T |
G |
19: 3,971,187 (GRCm39) |
D159A |
probably damaging |
Het |
| Ank1 |
T |
C |
8: 23,589,666 (GRCm39) |
V589A |
probably damaging |
Het |
| Ano2 |
G |
A |
6: 125,990,654 (GRCm39) |
D803N |
probably benign |
Het |
| Arid1b |
A |
G |
17: 5,393,241 (GRCm39) |
E2257G |
probably damaging |
Het |
| Asb17 |
T |
C |
3: 153,550,138 (GRCm39) |
Y57H |
probably benign |
Het |
| Asph |
T |
C |
4: 9,453,335 (GRCm39) |
E646G |
probably damaging |
Het |
| Aspm |
T |
A |
1: 139,405,832 (GRCm39) |
I1573K |
probably benign |
Het |
| Bcas1 |
C |
A |
2: 170,229,863 (GRCm39) |
D236Y |
probably damaging |
Het |
| Bcas2 |
G |
T |
3: 103,079,113 (GRCm39) |
G9* |
probably null |
Het |
| Btaf1 |
A |
G |
19: 36,964,030 (GRCm39) |
Q867R |
probably benign |
Het |
| Calhm3 |
C |
T |
19: 47,143,908 (GRCm39) |
V132I |
possibly damaging |
Het |
| Ccer1 |
A |
T |
10: 97,530,539 (GRCm39) |
I401F |
possibly damaging |
Het |
| Cdcp3 |
T |
C |
7: 130,839,818 (GRCm39) |
V580A |
probably damaging |
Het |
| Cecr2 |
T |
A |
6: 120,739,526 (GRCm39) |
|
probably benign |
Het |
| Cep104 |
G |
A |
4: 154,091,255 (GRCm39) |
R925Q |
possibly damaging |
Het |
| Cep164 |
T |
A |
9: 45,682,104 (GRCm39) |
M1900L |
probably benign |
Het |
| Crybg1 |
A |
G |
10: 43,873,673 (GRCm39) |
V1145A |
possibly damaging |
Het |
| Cyp2a4 |
T |
A |
7: 26,008,399 (GRCm39) |
N180K |
possibly damaging |
Het |
| Dennd4a |
T |
C |
9: 64,796,368 (GRCm39) |
L798P |
probably damaging |
Het |
| Dmxl1 |
T |
C |
18: 50,011,230 (GRCm39) |
I1129T |
probably benign |
Het |
| Dnah2 |
T |
C |
11: 69,406,578 (GRCm39) |
N555D |
possibly damaging |
Het |
| Dock1 |
T |
A |
7: 134,601,029 (GRCm39) |
M988K |
probably damaging |
Het |
| Elp4 |
T |
A |
2: 105,533,088 (GRCm39) |
H419L |
probably damaging |
Het |
| Endov |
T |
C |
11: 119,393,177 (GRCm39) |
V109A |
possibly damaging |
Het |
| Epha8 |
T |
C |
4: 136,663,625 (GRCm39) |
Y477C |
probably damaging |
Het |
| Erlin1 |
A |
G |
19: 44,037,561 (GRCm39) |
M188T |
probably damaging |
Het |
| Fhip1a |
T |
A |
3: 85,568,525 (GRCm39) |
D998V |
probably benign |
Het |
| Fhod3 |
A |
T |
18: 25,245,643 (GRCm39) |
D1231V |
possibly damaging |
Het |
| Gimap3 |
T |
A |
6: 48,742,646 (GRCm39) |
I95F |
possibly damaging |
Het |
| Gm10717 |
T |
G |
9: 3,026,317 (GRCm39) |
F205C |
probably damaging |
Het |
| Grk1 |
C |
A |
8: 13,457,923 (GRCm39) |
D274E |
probably damaging |
Het |
| Gsdmc2 |
G |
A |
15: 63,699,621 (GRCm39) |
A269V |
probably benign |
Het |
| Krt33a |
T |
A |
11: 99,903,175 (GRCm39) |
Q289L |
probably benign |
Het |
| Krt76 |
T |
A |
15: 101,796,600 (GRCm39) |
K403* |
probably null |
Het |
| Lcn4 |
T |
C |
2: 26,560,607 (GRCm39) |
|
probably benign |
Het |
| Mab21l1 |
T |
C |
3: 55,691,048 (GRCm39) |
S212P |
possibly damaging |
Het |
| Mapk8ip3 |
A |
C |
17: 25,123,025 (GRCm39) |
D610E |
probably benign |
Het |
| Mastl |
G |
T |
2: 23,022,692 (GRCm39) |
S677* |
probably null |
Het |
| Mfap3 |
T |
C |
11: 57,420,562 (GRCm39) |
F181S |
probably damaging |
Het |
| Mlkl |
T |
C |
8: 112,038,732 (GRCm39) |
|
probably benign |
Het |
| Mov10 |
G |
A |
3: 104,708,876 (GRCm39) |
|
probably benign |
Het |
| Muc5ac |
T |
C |
7: 141,350,041 (GRCm39) |
F595L |
probably benign |
Het |
| Nbas |
T |
A |
12: 13,616,145 (GRCm39) |
C2228S |
probably benign |
Het |
| Nit2 |
G |
A |
16: 56,982,046 (GRCm39) |
|
probably benign |
Het |
| Nod1 |
C |
T |
6: 54,921,425 (GRCm39) |
V298M |
probably damaging |
Het |
| Or3a4 |
C |
A |
11: 73,945,210 (GRCm39) |
R125L |
probably damaging |
Het |
| Or4c111 |
A |
G |
2: 88,843,565 (GRCm39) |
L281P |
probably damaging |
Het |
| Or52b1 |
T |
A |
7: 104,978,590 (GRCm39) |
I270F |
probably benign |
Het |
| Or6c210 |
A |
T |
10: 129,495,981 (GRCm39) |
D102V |
probably benign |
Het |
| Or7g12 |
T |
C |
9: 18,900,196 (GRCm39) |
L304P |
probably damaging |
Het |
| Osbpl5 |
C |
A |
7: 143,243,662 (GRCm39) |
R864L |
probably benign |
Het |
| Pcnt |
G |
A |
10: 76,204,650 (GRCm39) |
T2585M |
possibly damaging |
Het |
| Pepd |
C |
T |
7: 34,634,174 (GRCm39) |
|
probably benign |
Het |
| Pou6f2 |
T |
C |
13: 18,326,548 (GRCm39) |
I341V |
probably damaging |
Het |
| Pramel32 |
T |
C |
4: 88,548,309 (GRCm39) |
Q32R |
possibly damaging |
Het |
| Prune2 |
T |
A |
19: 17,091,038 (GRCm39) |
F281I |
probably benign |
Het |
| Psg19 |
T |
G |
7: 18,528,193 (GRCm39) |
Q183H |
probably damaging |
Het |
| Psme4 |
T |
G |
11: 30,765,658 (GRCm39) |
S587A |
probably benign |
Het |
| Ptpro |
T |
C |
6: 137,377,617 (GRCm39) |
|
probably benign |
Het |
| Pttg1ip2 |
C |
A |
5: 5,502,019 (GRCm39) |
W144C |
probably benign |
Het |
| Rasgrp4 |
T |
C |
7: 28,838,302 (GRCm39) |
V92A |
probably damaging |
Het |
| Rem1 |
G |
A |
2: 152,476,455 (GRCm39) |
V238M |
probably damaging |
Het |
| Rexo5 |
C |
T |
7: 119,398,867 (GRCm39) |
A68V |
probably damaging |
Het |
| Sfrp5 |
C |
T |
19: 42,187,237 (GRCm39) |
V278I |
probably benign |
Het |
| Sidt1 |
A |
G |
16: 44,102,234 (GRCm39) |
S309P |
possibly damaging |
Het |
| Slc22a6 |
A |
C |
19: 8,599,246 (GRCm39) |
Q292H |
probably benign |
Het |
| Slc4a3 |
G |
A |
1: 75,530,367 (GRCm39) |
R690H |
probably damaging |
Het |
| Snx7 |
A |
T |
3: 117,623,317 (GRCm39) |
|
probably null |
Het |
| Spag6 |
T |
A |
2: 18,720,616 (GRCm39) |
Y129* |
probably null |
Het |
| Srcap |
T |
C |
7: 127,133,994 (GRCm39) |
I905T |
probably damaging |
Het |
| St6gal1 |
T |
G |
16: 23,140,383 (GRCm39) |
S185A |
probably damaging |
Het |
| Sult6b1 |
G |
A |
17: 79,196,393 (GRCm39) |
H250Y |
possibly damaging |
Het |
| Tdrd6 |
T |
G |
17: 43,937,979 (GRCm39) |
N1023T |
probably benign |
Het |
| Tecta |
C |
T |
9: 42,249,232 (GRCm39) |
E1877K |
probably damaging |
Het |
| Tex10 |
C |
T |
4: 48,456,800 (GRCm39) |
R637Q |
probably benign |
Het |
| Tex14 |
T |
A |
11: 87,385,861 (GRCm39) |
D240E |
probably damaging |
Het |
| Tex47 |
T |
C |
5: 7,355,022 (GRCm39) |
Y68H |
probably damaging |
Het |
| Thbs2 |
A |
G |
17: 14,910,104 (GRCm39) |
V165A |
probably benign |
Het |
| Tmem126b |
T |
A |
7: 90,118,367 (GRCm39) |
Y171F |
possibly damaging |
Het |
| Tpsg1 |
G |
T |
17: 25,592,374 (GRCm39) |
M46I |
probably benign |
Het |
| Trmt2a |
A |
G |
16: 18,069,070 (GRCm39) |
K304R |
probably benign |
Het |
| Ttc28 |
G |
T |
5: 111,428,616 (GRCm39) |
R1845L |
possibly damaging |
Het |
| Umodl1 |
A |
T |
17: 31,211,128 (GRCm39) |
T884S |
possibly damaging |
Het |
| Vmn2r77 |
A |
G |
7: 86,461,001 (GRCm39) |
K776E |
probably damaging |
Het |
| Vmn2r88 |
T |
C |
14: 51,655,671 (GRCm39) |
S627P |
probably damaging |
Het |
| Vrk1 |
T |
C |
12: 106,024,236 (GRCm39) |
|
probably null |
Het |
| Zfp644 |
A |
G |
5: 106,783,137 (GRCm39) |
M1079T |
possibly damaging |
Het |
| Znrf1 |
T |
C |
8: 112,348,233 (GRCm39) |
*41Q |
probably null |
Het |
| Znrf1 |
T |
C |
8: 112,348,244 (GRCm39) |
F183L |
possibly damaging |
Het |
|
| Other mutations in Robo2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00666:Robo2
|
APN |
16 |
73,758,588 (GRCm39) |
missense |
probably benign |
|
|
IGL00849:Robo2
|
APN |
16 |
73,770,665 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
IGL00908:Robo2
|
APN |
16 |
73,782,579 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL00944:Robo2
|
APN |
16 |
73,730,585 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
IGL00955:Robo2
|
APN |
16 |
73,812,860 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00970:Robo2
|
APN |
16 |
73,693,934 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01020:Robo2
|
APN |
16 |
73,725,039 (GRCm39) |
missense |
probably benign |
0.06 |
|
IGL01347:Robo2
|
APN |
16 |
74,149,744 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02280:Robo2
|
APN |
16 |
73,843,704 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02424:Robo2
|
APN |
16 |
73,770,189 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
IGL03376:Robo2
|
APN |
16 |
73,753,380 (GRCm39) |
missense |
probably damaging |
1.00 |
|
LCD18:Robo2
|
UTSW |
16 |
74,055,954 (GRCm38) |
intron |
probably benign |
|
|
P0018:Robo2
|
UTSW |
16 |
73,843,694 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R0314:Robo2
|
UTSW |
16 |
73,753,525 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0324:Robo2
|
UTSW |
16 |
73,764,739 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0539:Robo2
|
UTSW |
16 |
73,782,462 (GRCm39) |
splice site |
probably benign |
|
|
R0620:Robo2
|
UTSW |
16 |
73,764,690 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R0630:Robo2
|
UTSW |
16 |
73,713,093 (GRCm39) |
missense |
probably benign |
0.05 |
|
R0701:Robo2
|
UTSW |
16 |
73,843,762 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1155:Robo2
|
UTSW |
16 |
73,831,996 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1168:Robo2
|
UTSW |
16 |
73,745,184 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1195:Robo2
|
UTSW |
16 |
73,713,016 (GRCm39) |
splice site |
probably null |
|
|
R1195:Robo2
|
UTSW |
16 |
73,713,016 (GRCm39) |
splice site |
probably null |
|
|
R1195:Robo2
|
UTSW |
16 |
73,713,016 (GRCm39) |
splice site |
probably null |
|
|
R1317:Robo2
|
UTSW |
16 |
73,831,912 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1422:Robo2
|
UTSW |
16 |
73,775,336 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1452:Robo2
|
UTSW |
16 |
73,758,798 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1649:Robo2
|
UTSW |
16 |
73,695,889 (GRCm39) |
missense |
probably benign |
0.36 |
|
R1709:Robo2
|
UTSW |
16 |
73,753,411 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R1751:Robo2
|
UTSW |
16 |
73,831,912 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1761:Robo2
|
UTSW |
16 |
73,831,912 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1885:Robo2
|
UTSW |
16 |
73,713,033 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1919:Robo2
|
UTSW |
16 |
73,696,042 (GRCm39) |
missense |
probably benign |
|
|
R2005:Robo2
|
UTSW |
16 |
73,730,003 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2851:Robo2
|
UTSW |
16 |
73,758,776 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3732:Robo2
|
UTSW |
16 |
73,717,635 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R3732:Robo2
|
UTSW |
16 |
73,717,635 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R3733:Robo2
|
UTSW |
16 |
73,717,635 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R3734:Robo2
|
UTSW |
16 |
73,717,635 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R3913:Robo2
|
UTSW |
16 |
73,831,893 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3956:Robo2
|
UTSW |
16 |
73,758,755 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4394:Robo2
|
UTSW |
16 |
73,745,267 (GRCm39) |
missense |
probably benign |
0.13 |
|
R4426:Robo2
|
UTSW |
16 |
73,745,154 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4437:Robo2
|
UTSW |
16 |
73,770,132 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R4454:Robo2
|
UTSW |
16 |
74,149,407 (GRCm39) |
intron |
probably benign |
|
|
R4478:Robo2
|
UTSW |
16 |
73,812,761 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4586:Robo2
|
UTSW |
16 |
73,758,761 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4621:Robo2
|
UTSW |
16 |
73,782,821 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4673:Robo2
|
UTSW |
16 |
73,701,266 (GRCm39) |
splice site |
probably null |
|
|
R4798:Robo2
|
UTSW |
16 |
74,149,633 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4812:Robo2
|
UTSW |
16 |
73,713,176 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4855:Robo2
|
UTSW |
16 |
73,768,079 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Robo2
|
UTSW |
16 |
73,730,666 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4916:Robo2
|
UTSW |
16 |
73,695,803 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R4948:Robo2
|
UTSW |
16 |
74,149,726 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R5325:Robo2
|
UTSW |
16 |
73,770,673 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R5326:Robo2
|
UTSW |
16 |
73,695,853 (GRCm39) |
missense |
probably benign |
0.20 |
|
R5447:Robo2
|
UTSW |
16 |
73,770,654 (GRCm39) |
nonsense |
probably null |
|
|
R5542:Robo2
|
UTSW |
16 |
73,695,853 (GRCm39) |
missense |
probably benign |
0.20 |
|
R5545:Robo2
|
UTSW |
16 |
73,758,635 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5646:Robo2
|
UTSW |
16 |
73,758,707 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5734:Robo2
|
UTSW |
16 |
74,149,672 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5892:Robo2
|
UTSW |
16 |
73,692,668 (GRCm39) |
utr 3 prime |
probably benign |
|
|
R5960:Robo2
|
UTSW |
16 |
73,730,603 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6126:Robo2
|
UTSW |
16 |
73,717,570 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6130:Robo2
|
UTSW |
16 |
73,717,570 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6153:Robo2
|
UTSW |
16 |
73,717,617 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6240:Robo2
|
UTSW |
16 |
73,779,027 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6247:Robo2
|
UTSW |
16 |
73,764,672 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6304:Robo2
|
UTSW |
16 |
73,755,196 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6337:Robo2
|
UTSW |
16 |
73,725,039 (GRCm39) |
missense |
probably benign |
0.06 |
|
R6431:Robo2
|
UTSW |
16 |
73,843,697 (GRCm39) |
nonsense |
probably null |
|
|
R6440:Robo2
|
UTSW |
16 |
73,713,010 (GRCm39) |
missense |
probably benign |
0.31 |
|
R6596:Robo2
|
UTSW |
16 |
73,767,996 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6919:Robo2
|
UTSW |
16 |
73,758,755 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6927:Robo2
|
UTSW |
16 |
73,778,946 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7029:Robo2
|
UTSW |
16 |
73,745,225 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7078:Robo2
|
UTSW |
16 |
74,149,504 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7092:Robo2
|
UTSW |
16 |
73,753,531 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7136:Robo2
|
UTSW |
16 |
73,753,438 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7192:Robo2
|
UTSW |
16 |
73,717,638 (GRCm39) |
missense |
probably benign |
0.19 |
|
R7569:Robo2
|
UTSW |
16 |
73,832,003 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R7686:Robo2
|
UTSW |
16 |
73,755,293 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7720:Robo2
|
UTSW |
16 |
73,693,903 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7772:Robo2
|
UTSW |
16 |
73,758,777 (GRCm39) |
missense |
probably benign |
0.24 |
|
R7822:Robo2
|
UTSW |
16 |
73,770,059 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7849:Robo2
|
UTSW |
16 |
73,770,132 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R7881:Robo2
|
UTSW |
16 |
73,717,585 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7897:Robo2
|
UTSW |
16 |
73,695,838 (GRCm39) |
missense |
probably benign |
|
|
R8135:Robo2
|
UTSW |
16 |
73,730,048 (GRCm39) |
missense |
probably benign |
0.04 |
|
R8297:Robo2
|
UTSW |
16 |
73,812,814 (GRCm39) |
nonsense |
probably null |
|
|
R8307:Robo2
|
UTSW |
16 |
73,753,498 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8379:Robo2
|
UTSW |
16 |
73,730,588 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8393:Robo2
|
UTSW |
16 |
73,775,382 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8474:Robo2
|
UTSW |
16 |
73,745,150 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8500:Robo2
|
UTSW |
16 |
73,745,228 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8721:Robo2
|
UTSW |
16 |
73,703,798 (GRCm39) |
missense |
|
|
|
R8734:Robo2
|
UTSW |
16 |
73,764,651 (GRCm39) |
splice site |
probably benign |
|
|
R8735:Robo2
|
UTSW |
16 |
73,755,247 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8840:Robo2
|
UTSW |
16 |
73,782,570 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8937:Robo2
|
UTSW |
16 |
73,770,150 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8937:Robo2
|
UTSW |
16 |
73,770,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8968:Robo2
|
UTSW |
16 |
73,767,941 (GRCm39) |
critical splice donor site |
probably null |
|
|
R9134:Robo2
|
UTSW |
16 |
73,703,738 (GRCm39) |
missense |
|
|
|
R9622:Robo2
|
UTSW |
16 |
73,729,952 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9662:Robo2
|
UTSW |
16 |
73,758,566 (GRCm39) |
critical splice donor site |
probably null |
|
|
R9708:Robo2
|
UTSW |
16 |
73,770,197 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R9779:Robo2
|
UTSW |
16 |
73,767,965 (GRCm39) |
missense |
probably damaging |
0.97 |
|
X0063:Robo2
|
UTSW |
16 |
73,842,716 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Robo2
|
UTSW |
16 |
73,730,479 (GRCm39) |
missense |
probably benign |
0.35 |
|
Z1177:Robo2
|
UTSW |
16 |
73,737,187 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TTGTGTACCAATGGCCCTAAG -3'
(R):5'- GGCAGAACCTTAAAAGTCTTGTC -3'
Sequencing Primer
(F):5'- TTGTGTACCAATGGCCCTAAGATCAC -3'
(R):5'- TGTCTTTATTTTCATGGTCAGTAAGC -3'
|
| Posted On |
2014-06-30 |
Incidental Mutation