Mutagenetix > Incidental Mutation

Incidental Mutation 'R1869:Vmn2r28'

210530

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

039891-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R1869 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

5479531-5493967 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 5486346 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 498 (Y498C)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably benign
Transcript: ENSMUST00000086297
AA Change: Y498C

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: Y498C

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.4%
20x: 92.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 127 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921536K21Rik	A	G	11: 3,890,067	L110P	probably damaging	Het
6030458C11Rik	T	C	15: 12,817,907	D168G	probably damaging	Het
Abca13	A	T	11: 9,292,134	E1332D	probably benign	Het
Abhd11	A	G	5: 135,011,617	Y168C	probably damaging	Het
Actr5	A	C	2: 158,638,723	T554P	probably damaging	Het
Adamts16	A	G	13: 70,735,747	L1103P	probably damaging	Het
Adgrb3	A	T	1: 25,826,438	I108K	possibly damaging	Het
Agbl4	T	A	4: 111,566,629	M284K	possibly damaging	Het
Aoc3	T	A	11: 101,331,467	Y176*	probably null	Het
Aox1	A	G	1: 58,076,103	E749G	probably damaging	Het
Ap4b1	T	A	3: 103,820,868	Y399*	probably null	Het
Arhgef5	A	T	6: 43,288,682	I1553F	probably damaging	Het
Bag6	T	C	17: 35,142,826	V541A	probably benign	Het
Bdp1	A	C	13: 100,042,201	V1879G	probably damaging	Het
Bod1l	G	A	5: 41,833,675	S179F	possibly damaging	Het
Bsnd	C	T	4: 106,486,636	V158I	probably benign	Het
Cachd1	C	A	4: 100,983,390	H880N	probably damaging	Het
Ccdc27	T	C	4: 154,026,763		probably null	Het
Cd74	C	T	18: 60,810,412	P190S	probably benign	Het
Cep68	A	T	11: 20,240,217	L265Q	probably damaging	Het
Cfap70	G	A	14: 20,408,610	Q772*	probably null	Het
CK137956	T	A	4: 127,970,534	S101C	possibly damaging	Het
Clca3a2	G	A	3: 144,806,403	T524M	probably benign	Het
Cxcr6	T	C	9: 123,809,957	S15P	probably benign	Het
Cyfip2	T	A	11: 46,224,168	Y904F	probably benign	Het
D130040H23Rik	T	A	8: 69,302,702	I253N	probably benign	Het
Ddx56	C	T	11: 6,263,993	V389M	possibly damaging	Het
Dmp1	A	T	5: 104,212,076	D206V	probably damaging	Het
Dnah17	A	T	11: 118,047,189	C3350*	probably null	Het
Dpcr1	A	G	17: 35,638,413	L98P	possibly damaging	Het
Dst	A	T	1: 34,252,832	S877C	probably damaging	Het
Ermp1	A	G	19: 29,646,015	S192P	possibly damaging	Het
F13b	G	A	1: 139,510,934	D359N	probably benign	Het
Fam171a1	A	T	2: 3,226,152	Q769L	possibly damaging	Het
Fap	T	C	2: 62,528,727	R411G	probably damaging	Het
Fbn1	A	G	2: 125,352,027	V1340A	probably benign	Het
Fbxw19	T	C	9: 109,482,032	I370V	probably benign	Het
Foxi1	A	T	11: 34,207,937	N29K	possibly damaging	Het
Frem2	T	G	3: 53,535,196	D2640A	probably benign	Het
Fzd4	T	C	7: 89,407,246	V167A	probably benign	Het
Galnt3	T	C	2: 66,097,779	I258V	possibly damaging	Het
Galr2	T	C	11: 116,283,243	V233A	possibly damaging	Het
Gldc	G	T	19: 30,139,332	Q407K	probably benign	Het
Gm5346	A	T	8: 43,625,095	Y697*	probably null	Het
Gmcl1	T	C	6: 86,697,516	D460G	probably benign	Het
Gpr89	A	G	3: 96,875,659	I325T	probably benign	Het
Grap	T	A	11: 61,664,189	Y61N	possibly damaging	Het
Hbs1l	G	T	10: 21,358,406		probably null	Het
Hexb	T	A	13: 97,191,259	E134D	probably benign	Het
Hydin	T	A	8: 110,500,705	V1507D	possibly damaging	Het
Igkv8-16	C	A	6: 70,386,987	G17V	probably benign	Het
Kdm4a	T	A	4: 118,138,871	Q987L	probably null	Het
Krt76	A	G	15: 101,889,487		probably null	Het
Lap3	T	C	5: 45,503,387	S238P	probably benign	Het
Letm2	T	C	8: 25,581,713	D391G	probably damaging	Het
Lipn	A	G	19: 34,080,739	T287A	possibly damaging	Het
Macf1	T	C	4: 123,351,128	S466G	probably damaging	Het
Map3k5	C	A	10: 20,132,109	S1220*	probably null	Het
Map4	T	C	9: 110,034,964	V419A	probably benign	Het
Map4	T	A	9: 110,068,928	S754R	possibly damaging	Het
Mroh9	A	G	1: 163,026,513	S773P	probably damaging	Het
Mtus1	A	C	8: 41,076,230		probably null	Het
Muc1	C	T	3: 89,231,810	P533S	probably damaging	Het
Muc13	A	T	16: 33,804,600	I282F	probably damaging	Het
Myh14	T	A	7: 44,611,643	Q1816L	possibly damaging	Het
Nalcn	A	C	14: 123,594,553	V103G	possibly damaging	Het
Nlrp9c	G	A	7: 26,384,820	Q445*	probably null	Het
Olfml2b	A	G	1: 170,669,243	D481G	probably damaging	Het
Olfr1000	A	G	2: 85,608,500	S137P	probably benign	Het
Olfr1087	T	A	2: 86,690,391	I195F	probably damaging	Het
Olfr309	T	A	7: 86,306,667	I149F	possibly damaging	Het
Olfr703	T	G	7: 106,845,094	I161S	probably benign	Het
Olfr884	T	A	9: 38,048,202		probably null	Het
Pcdhb2	A	T	18: 37,297,355		probably null	Het
Pcdhb22	A	C	18: 37,519,147	T223P	probably damaging	Het
Pcdhga1	T	A	18: 37,840,090	N907K	probably damaging	Het
Pcnt	T	C	10: 76,379,906	E2347G	probably null	Het
Phax	T	A	18: 56,573,104	V22D	probably benign	Het
Pik3ca	T	C	3: 32,450,350	F667S	probably damaging	Het
Pkd1	T	C	17: 24,594,931	F4123S	probably damaging	Het
Pkhd1	A	G	1: 20,615,267		probably null	Het
Plcb1	G	A	2: 135,311,014	R368K	probably benign	Het
Prrc2a	G	T	17: 35,153,308	R1739S	possibly damaging	Het
Psg23	A	T	7: 18,614,618	M88K	probably benign	Het
Pstk	T	A	7: 131,387,797	Y354N	probably damaging	Het
Rab39	A	G	9: 53,705,931	I62T	possibly damaging	Het
Ranbp2	A	G	10: 58,492,561	I2800V	probably damaging	Het
Rasgrp1	T	C	2: 117,290,347	Y431C	probably damaging	Het
Rest	T	A	5: 77,268,362	V141E	possibly damaging	Het
Rnf167	T	A	11: 70,650,139	I197N	possibly damaging	Het
Rtn1	A	G	12: 72,308,168	S335P	probably damaging	Het
Ryr2	G	T	13: 11,662,075	R3297S	probably damaging	Het
S1pr3	A	C	13: 51,419,916	K378Q	probably benign	Het
Scgb1b24	T	C	7: 33,744,044	Y43H	probably damaging	Het
Sh3rf3	T	A	10: 59,083,513	M484K	probably damaging	Het
Shank1	A	T	7: 44,342,115	R69*	probably null	Het
Siglecf	A	G	7: 43,355,543	N399S	probably benign	Het
Slc1a7	A	G	4: 108,008,364	T350A	probably damaging	Het
Slc25a54	T	A	3: 109,080,616	Y24*	probably null	Het
Slk	T	C	19: 47,625,452	F916L	probably damaging	Het
Snap91	T	A	9: 86,790,141		probably null	Het
Snrpb2	A	T	2: 143,065,186	R25S	possibly damaging	Het
Specc1l	T	C	10: 75,261,825	Y811H	probably damaging	Het
Sprr2h	A	G	3: 92,386,618		probably benign	Het
Sult2a5	A	T	7: 13,624,120	I26F	probably benign	Het
Syt13	C	T	2: 92,946,103	T245I	possibly damaging	Het
Tecrl	T	A	5: 83,354,859	T33S	probably benign	Het
Telo2	T	A	17: 25,114,994	D93V	probably benign	Het
Tet2	A	T	3: 133,481,441		probably null	Het
Tgm7	A	T	2: 121,101,089	C198S	probably damaging	Het
Ticrr	T	C	7: 79,679,135	F674S	probably damaging	Het
Tmem129	C	A	5: 33,654,499	C31F	probably damaging	Het
Tmem132a	T	A	19: 10,858,688	D826V	possibly damaging	Het
Tpd52	T	C	3: 8,953,802		probably null	Het
Trub2	A	G	2: 29,777,666	S321P	probably benign	Het
Ttc29	A	G	8: 78,282,385	T295A	probably benign	Het
Tubb1	G	A	2: 174,456,689	S89N	probably benign	Het
Ubap2	C	A	4: 41,233,617	G91V	probably damaging	Het
Ugt2b35	A	G	5: 87,001,288	N133D	probably damaging	Het
Usp34	A	G	11: 23,364,479	H807R	probably benign	Het
Vmn1r220	A	C	13: 23,184,287	C80G	probably damaging	Het
Vmn1r79	A	G	7: 12,176,647	E152G	probably benign	Het
Wif1	A	G	10: 121,084,919	I215M	probably benign	Het
Wnk1	T	C	6: 119,951,089	T1134A	probably damaging	Het
Zcchc11	T	A	4: 108,529,300	F1122L	probably damaging	Het
Zfp90	A	G	8: 106,419,123	H29R	probably benign	Het
Zfp972	A	G	2: 177,921,687		probably null	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5488069	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5488184	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5486478	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5481088	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5486464	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5488162	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5481303	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5488124	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5490569	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5481004	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5484016	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5488021	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5493716	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5488514	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5490457	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5488690	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5481302	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5481545	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5493811	missense	probably benign
R1853:Vmn2r28	UTSW	7	5481247	nonsense	probably null
R1887:Vmn2r28	UTSW	7	5488289	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5481071	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5488314	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5484131	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5488702	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5481392	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5488358	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5490708	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5480672	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5486377	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5487944	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5484012	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5488669	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5493791	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5488004	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5490731	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5488475	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5486436	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5490685	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5481230	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5480807	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5490734	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5481496	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5481309	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5488201	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5481256	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5493679	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5490614	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5480819	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5488148	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5484060	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5486348	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5490474	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5488597	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5480748	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5484119	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5488221	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CAAACAGTGGTGAAGAACAGTTTTG -3'
(R):5'- GAGGAAGACCCACTTTACTAATCCTC -3'

Sequencing Primer
(F):5'- TCTTCAAAGAATTGGGGAAAATGTC -3'
(R):5'- AGACCCACTTTACTAATCCTCTTGGG -3'

Posted On

2014-06-30