Mutagenetix > Incidental Mutation

Incidental Mutation 'R0121:Zfp944'

21058

Institutional Source

Beutler Lab

Gene Symbol

Zfp944

Ensembl Gene

ENSMUSG00000033972

Gene Name

zinc finger protein 944

Synonyms

6330416L07Rik

MMRRC Submission

038406-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.223) question?

Stock #

R0121 (G1)

Quality Score

209

Status

Validated (trace)

Chromosome

Chromosomal Location

22556970-22580381 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 22558249 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 333 (T333S)

Ref Sequence

ENSEMBL: ENSMUSP00000111197 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000115535]

AlphaFold

E9PUS4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000115535
AA Change: T333S

PolyPhen 2 Score 0.692 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000111197
Gene: ENSMUSG00000033972
AA Change: T333S

Domain	Start	End	E-Value	Type
KRAB	13	76	2.08e-21	SMART
ZnF_C2H2	183	205	1.01e-1	SMART
ZnF_C2H2	211	233	1.07e0	SMART
ZnF_C2H2	239	261	1.95e-3	SMART
ZnF_C2H2	267	289	1.22e-4	SMART
ZnF_C2H2	295	317	2.24e-3	SMART
ZnF_C2H2	323	345	1.82e-3	SMART
ZnF_C2H2	351	373	5.99e-4	SMART
ZnF_C2H2	379	401	4.79e-3	SMART
ZnF_C2H2	407	429	2.99e-4	SMART
ZnF_C2H2	435	457	4.17e-3	SMART
ZnF_C2H2	463	485	1.36e-2	SMART

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.5%
20x: 89.8%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,298,945 (GRCm39)		probably null	Het
Adgra3	T	C	5: 50,183,128 (GRCm39)		probably benign	Het
Anxa7	A	T	14: 20,510,227 (GRCm39)	L386M	probably damaging	Het
Ap2b1	A	G	11: 83,212,793 (GRCm39)	M58V	possibly damaging	Het
Arfip2	A	G	7: 105,285,578 (GRCm39)	L224P	probably damaging	Het
Arhgap20	A	G	9: 51,750,251 (GRCm39)	N373S	possibly damaging	Het
Asph	T	C	4: 9,635,918 (GRCm39)	D73G	probably damaging	Het
Atp1a2	T	A	1: 172,116,909 (GRCm39)	E236V	probably damaging	Het
Atp2a1	A	G	7: 126,057,116 (GRCm39)	S170P	probably damaging	Het
Atp4a	C	G	7: 30,419,526 (GRCm39)	R659G	probably benign	Het
B4galnt3	C	T	6: 120,191,999 (GRCm39)	R578H	probably benign	Het
Ccdc178	C	A	18: 21,978,081 (GRCm39)		probably null	Het
Ccnh	T	A	13: 85,354,312 (GRCm39)	M252K	probably damaging	Het
Clec4b2	A	G	6: 123,181,131 (GRCm39)	D172G	probably benign	Het
Col1a1	A	G	11: 94,828,895 (GRCm39)	E79G	unknown	Het
Csf3r	A	G	4: 125,923,642 (GRCm39)	N51D	probably benign	Het
Cul7	C	T	17: 46,974,299 (GRCm39)	L1489F	probably damaging	Het
Cyp2b13	G	A	7: 25,786,010 (GRCm39)	C309Y	probably benign	Het
Dync2h1	A	T	9: 7,001,327 (GRCm39)		probably benign	Het
Edn1	A	G	13: 42,458,741 (GRCm39)	T135A	probably benign	Het
Ephb2	A	G	4: 136,498,368 (GRCm39)	I237T	probably damaging	Het
Fam111a	T	A	19: 12,561,444 (GRCm39)	F12L	probably benign	Het
Foxi2	C	A	7: 135,013,640 (GRCm39)	A290E	probably benign	Het
Gabra6	A	G	11: 42,205,798 (GRCm39)	S353P	probably benign	Het
Gm4847	T	C	1: 166,469,857 (GRCm39)	D72G	probably damaging	Het
Grhl3	A	G	4: 135,279,860 (GRCm39)	I398T	probably damaging	Het
Gtdc1	T	C	2: 44,455,550 (GRCm39)		probably benign	Het
Kel	A	C	6: 41,678,998 (GRCm39)		probably benign	Het
L3mbtl3	C	T	10: 26,189,768 (GRCm39)	D499N	unknown	Het
Lama1	T	A	17: 68,105,508 (GRCm39)		probably benign	Het
Mamdc2	T	A	19: 23,288,223 (GRCm39)	E605V	probably benign	Het
Nolc1	G	A	19: 46,069,817 (GRCm39)		probably benign	Het
Nudt12	A	T	17: 59,314,634 (GRCm39)	S317T	possibly damaging	Het
Optn	C	T	2: 5,028,926 (GRCm39)	G526R	probably damaging	Het
Or4k35	A	T	2: 111,100,659 (GRCm39)	C18S	probably benign	Het
Or5w20	A	G	2: 87,727,434 (GRCm39)	K297R	possibly damaging	Het
Or8g23	T	A	9: 38,971,056 (GRCm39)	K302M	probably damaging	Het
Or8k38	A	G	2: 86,488,163 (GRCm39)	V213A	probably benign	Het
Pbld1	C	T	10: 62,907,282 (GRCm39)		probably benign	Het
Prl8a9	T	G	13: 27,744,589 (GRCm39)	N84T	probably benign	Het
Psph	T	A	5: 129,868,633 (GRCm39)		probably benign	Het
Sbf2	A	G	7: 110,088,426 (GRCm39)		probably null	Het
Senp6	A	G	9: 80,023,952 (GRCm39)	D405G	probably benign	Het
Serpinb1a	T	A	13: 33,032,754 (GRCm39)		probably benign	Het
Slc2a9	T	C	5: 38,556,086 (GRCm39)	I287V	probably benign	Het
Sptbn2	T	C	19: 4,795,321 (GRCm39)	F1593S	probably damaging	Het
Tcf21	T	C	10: 22,695,706 (GRCm39)	T33A	probably benign	Het
Tdrd3	A	T	14: 87,776,915 (GRCm39)	Q727L	probably damaging	Het
Tecpr1	C	T	5: 144,147,017 (GRCm39)	E450K	probably benign	Het
Tenm3	G	A	8: 48,795,694 (GRCm39)	T532I	probably damaging	Het
Tg	A	T	15: 66,612,630 (GRCm39)	Q396L	probably benign	Het
Tmtc3	A	G	10: 100,294,770 (GRCm39)		probably benign	Het
Twnk	T	C	19: 44,997,704 (GRCm39)		probably benign	Het
Ubac1	A	G	2: 25,898,871 (GRCm39)		probably null	Het
Ubn2	T	C	6: 38,429,793 (GRCm39)		probably benign	Het

Other mutations in Zfp944

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00595:Zfp944	APN	17	22,558,186 (GRCm39)	missense	probably benign	0.10
IGL00917:Zfp944	APN	17	22,558,765 (GRCm39)	missense	probably benign	0.00
IGL01093:Zfp944	APN	17	22,562,615 (GRCm39)	splice site	probably benign
IGL02113:Zfp944	APN	17	22,558,047 (GRCm39)	missense	possibly damaging	0.88
IGL02694:Zfp944	APN	17	22,558,899 (GRCm39)	missense	probably benign	0.05
IGL03135:Zfp944	APN	17	22,558,737 (GRCm39)	missense	probably benign	0.00
IGL03172:Zfp944	APN	17	22,559,018 (GRCm39)	missense	probably damaging	0.98
R0336:Zfp944	UTSW	17	22,558,009 (GRCm39)	missense	probably damaging	1.00
R0755:Zfp944	UTSW	17	22,558,889 (GRCm39)	missense	possibly damaging	0.63
R1536:Zfp944	UTSW	17	22,558,697 (GRCm39)	nonsense	probably null
R1708:Zfp944	UTSW	17	22,558,026 (GRCm39)	missense	probably damaging	0.98
R1886:Zfp944	UTSW	17	22,558,960 (GRCm39)	missense	probably benign	0.04
R1928:Zfp944	UTSW	17	22,560,065 (GRCm39)	missense	probably damaging	0.96
R1950:Zfp944	UTSW	17	22,558,681 (GRCm39)	missense	probably benign	0.16
R2075:Zfp944	UTSW	17	22,558,178 (GRCm39)	nonsense	probably null
R2101:Zfp944	UTSW	17	22,558,809 (GRCm39)	missense	probably benign	0.41
R2433:Zfp944	UTSW	17	22,558,193 (GRCm39)	nonsense	probably null
R4698:Zfp944	UTSW	17	22,558,180 (GRCm39)	missense	probably damaging	1.00
R4986:Zfp944	UTSW	17	22,558,211 (GRCm39)	missense	probably damaging	1.00
R6451:Zfp944	UTSW	17	22,557,846 (GRCm39)	missense	probably benign	0.40
R6566:Zfp944	UTSW	17	22,558,726 (GRCm39)	missense	possibly damaging	0.96
R6752:Zfp944	UTSW	17	22,558,500 (GRCm39)	missense	probably benign	0.01
R7064:Zfp944	UTSW	17	22,558,560 (GRCm39)	missense	probably damaging	1.00
R8193:Zfp944	UTSW	17	22,558,861 (GRCm39)	nonsense	probably null
R8323:Zfp944	UTSW	17	22,558,235 (GRCm39)	missense	probably benign
R8328:Zfp944	UTSW	17	22,558,705 (GRCm39)	nonsense	probably null
R8902:Zfp944	UTSW	17	22,558,761 (GRCm39)	missense	probably benign	0.41
R8915:Zfp944	UTSW	17	22,558,507 (GRCm39)	missense	probably benign	0.05
R9130:Zfp944	UTSW	17	22,560,031 (GRCm39)	missense	probably damaging	1.00
R9248:Zfp944	UTSW	17	22,562,619 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- ACATGCCACATGTTTTCCAGCAGa -3'
(R):5'- AATACTCGGGCTGAAACAAACCAATAGT -3'

Sequencing Primer
(F):5'- gggtttgtgtcatttgtttcatttg -3'
(R):5'- caatgtgacaaatgcttttccc -3'

Posted On

2013-04-11