Mutagenetix > Incidental Mutation

Incidental Mutation 'R1902:Rif1'

212288

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

039922-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1902 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 52116673 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 2206 (N2206K)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000069794
AA Change: N2206K

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: N2206K

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112693
AA Change: N2206K

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: N2206K

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125322

Predicted Effect

unknown
Transcript: ENSMUST00000125376
AA Change: N564K

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Coding Region Coverage

1x: 97.3%
3x: 96.7%
10x: 94.9%
20x: 91.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 128 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730507C01Rik	C	T	12: 18,534,003	Q355*	probably null	Het
Acacb	A	T	5: 114,165,734	R73*	probably null	Het
Adra1a	T	C	14: 66,638,235	S220P	probably benign	Het
Ano2	A	T	6: 125,872,684	E126D	probably damaging	Het
Anpep	C	T	7: 79,838,256	E518K	probably benign	Het
Arhgap45	C	A	10: 80,025,466	Q488K	probably damaging	Het
Arhgef7	C	A	8: 11,808,713		probably null	Het
Atxn7l3b	A	G	10: 112,928,673	I17T	probably benign	Het
BC003331	T	A	1: 150,388,609		probably null	Het
Bpifb9a	A	T	2: 154,261,991	N118I	probably benign	Het
Btbd9	A	T	17: 30,530,228	D37E	probably damaging	Het
C8a	T	C	4: 104,856,601		probably null	Het
Capn13	G	A	17: 73,326,361	S535F	probably damaging	Het
Carns1	G	T	19: 4,166,338	P615Q	probably damaging	Het
Casz1	T	C	4: 148,936,195	I479T	possibly damaging	Het
Ccdc88a	G	T	11: 29,461,788	M532I	probably benign	Het
Cdc42bpb	T	G	12: 111,326,016	S362R	probably damaging	Het
Ceacam11	A	T	7: 17,975,327	H150L	probably benign	Het
Cfap44	A	C	16: 44,422,374	T714P	probably benign	Het
Cnot1	T	C	8: 95,743,121	I1369V	possibly damaging	Het
Cpeb1	T	C	7: 81,372,119	D92G	probably benign	Het
Cped1	T	A	6: 22,120,981		probably null	Het
Csf1r	A	G	18: 61,130,141	T896A	probably damaging	Het
Csf3r	T	C	4: 126,042,918	F658S	probably damaging	Het
Cts6	A	T	13: 61,201,515	Y126*	probably null	Het
Cul7	T	C	17: 46,655,740	L365P	probably damaging	Het
Cxcl5	T	C	5: 90,759,785	V72A	probably damaging	Het
Cyp4v3	T	A	8: 45,306,952	H521L	probably benign	Het
Dct	T	C	14: 118,034,278	N380S	probably benign	Het
Dnah1	T	C	14: 31,319,759	D85G	probably damaging	Het
Dnah7a	T	C	1: 53,535,478	D1709G	probably damaging	Het
Dnaic1	A	T	4: 41,625,319	K428*	probably null	Het
Dsc1	A	T	18: 20,095,988	V415D	probably damaging	Het
E2f8	T	C	7: 48,871,172	H467R	probably benign	Het
Eddm3b	T	A	14: 51,116,864	I103N	probably damaging	Het
Fam71b	A	T	11: 46,407,011	T381S	probably benign	Het
Gal3st2c	T	G	1: 94,008,889	N185K	probably damaging	Het
Ganc	T	C	2: 120,446,482	L675P	probably damaging	Het
Gdf9	G	A	11: 53,436,953	M245I	probably benign	Het
Gm21886	G	A	18: 80,089,418	T175I	probably damaging	Het
Gria2	A	G	3: 80,722,108	L269P	probably damaging	Het
Grm8	T	C	6: 27,429,482	Y471C	probably damaging	Het
Gucy2g	T	A	19: 55,210,237	T825S	probably benign	Het
H2-Bl	A	T	17: 36,083,953	M16K	probably damaging	Het
Iffo2	T	C	4: 139,607,701	S124P	probably damaging	Het
Igf1r	T	C	7: 68,201,249	Y931H	possibly damaging	Het
Il22ra1	A	T	4: 135,750,908	Q430L	probably damaging	Het
Il23r	A	C	6: 67,423,734	D537E	probably benign	Het
Ip6k1	G	A	9: 108,040,996	E77K	possibly damaging	Het
Itgb4	T	C	11: 115,980,738	V179A	probably damaging	Het
Itpr2	T	A	6: 146,229,703	H1968L	probably damaging	Het
Kdm4a	A	G	4: 118,160,399	V490A	probably benign	Het
Kif13a	A	T	13: 46,788,162	D946E	probably benign	Het
Klhl22	T	A	16: 17,771,787	I104N	probably damaging	Het
Klk6	A	C	7: 43,826,057	M1L	probably benign	Het
Krt78	A	T	15: 101,946,963	C804*	probably null	Het
Larp7	A	T	3: 127,540,578	N533K	probably damaging	Het
Lrit3	T	C	3: 129,791,246	T288A	probably benign	Het
Lrp11	T	A	10: 7,623,780	L245Q	probably damaging	Het
Lrp1b	T	C	2: 40,860,661	S2964G	probably damaging	Het
Lrrc66	C	T	5: 73,607,622	V693M	probably damaging	Het
Macf1	T	A	4: 123,471,165	M3268L	probably benign	Het
Mad1l1	A	G	5: 140,303,688	S161P	possibly damaging	Het
Mettl25	A	G	10: 105,826,087	S341P	probably damaging	Het
Morc3	A	G	16: 93,870,497	T588A	probably damaging	Het
Mospd3	A	G	5: 137,600,415	S21P	probably damaging	Het
Mrpl57	T	A	14: 57,826,729	F71L	probably damaging	Het
Mtbp	T	C	15: 55,606,715	L594S	probably damaging	Het
Mthfd2	T	C	6: 83,306,731	N323S	probably damaging	Het
Muc5b	G	T	7: 141,864,105	S3596I	possibly damaging	Het
Mug1	A	G	6: 121,881,821	D1166G	probably benign	Het
Nav1	T	G	1: 135,472,410	N474T	probably benign	Het
Ncoa1	A	T	12: 4,339,049	D75E	possibly damaging	Het
Ncor1	A	C	11: 62,338,158	I958S	probably damaging	Het
Nefm	T	C	14: 68,124,114	S234G	probably benign	Het
Nlrp4c	T	C	7: 6,065,819	S240P	probably damaging	Het
Nox3	C	T	17: 3,670,017	V298M	probably damaging	Het
Ntn4	A	G	10: 93,707,372	D320G	possibly damaging	Het
Nudt13	C	G	14: 20,310,641	T174R	probably damaging	Het
Olfr1002	T	C	2: 85,647,857	S155G	possibly damaging	Het
Olfr119	T	A	17: 37,701,421	H250Q	probably damaging	Het
Olfr1245	A	T	2: 89,575,603	L41Q	possibly damaging	Het
Olfr1512	T	G	14: 52,372,717	Q112P	possibly damaging	Het
Olfr209	A	T	16: 59,362,163	D18E	probably benign	Het
Olfr355	T	C	2: 36,927,185	I310V	probably benign	Het
Olfr430	T	C	1: 174,070,126	V276A	probably damaging	Het
Olfr437	T	A	6: 43,167,723		probably null	Het
Olfr812	G	T	10: 129,842,506	P179T	probably benign	Het
Olfr967	T	C	9: 39,750,806	V140A	probably benign	Het
Opn1sw	T	A	6: 29,379,804	N144Y	possibly damaging	Het
Osbpl5	T	C	7: 143,703,181	D404G	possibly damaging	Het
Parp14	C	T	16: 35,853,518		probably null	Het
Pde3a	C	A	6: 141,498,770	N1101K	probably benign	Het
Plcb1	G	A	2: 134,813,613	V38I	possibly damaging	Het
Plec	T	A	15: 76,175,551	E3417D	probably damaging	Het
Ppfibp2	C	T	7: 107,746,378	P869L	probably damaging	Het
Ppp1r12a	T	A	10: 108,198,891	I99N	probably damaging	Het
Pramel5	G	A	4: 144,273,863	Q48*	probably null	Het
Prr13	C	A	15: 102,460,698		probably benign	Het
Psg22	T	A	7: 18,724,438	Y312*	probably null	Het
Ptchd4	A	T	17: 42,503,616	I803L	probably benign	Het
Rasa4	A	G	5: 136,091,238	D56G	probably benign	Het
Robo1	A	G	16: 72,960,204	Q351R	probably null	Het
Samd4	T	C	14: 47,074,128	F81S	probably damaging	Het
Sh3pxd2b	T	C	11: 32,423,559	*909Q	probably null	Het
Slc25a45	A	G	19: 5,884,522	R173G	probably damaging	Het
Smarca1	G	A	X: 47,849,963	Q723*	probably null	Het
Smoc1	T	C	12: 81,104,671	I54T	probably benign	Het
Spef2	G	T	15: 9,607,377	R1319S	probably damaging	Het
Steap3	T	G	1: 120,241,734	I240L	probably benign	Het
Stmn4	C	T	14: 66,355,609	L13F	probably damaging	Het
Stxbp5	C	A	10: 9,812,298	V420F	possibly damaging	Het
Syt3	T	C	7: 44,390,516	S58P	possibly damaging	Het
Tas2r125	T	C	6: 132,910,176	F176L	probably benign	Het
Tbc1d19	T	A	5: 53,829,353	C35S	probably benign	Het
Tcaf3	T	C	6: 42,593,552	E422G	possibly damaging	Het
Tekt4	T	A	17: 25,471,858	F46Y	possibly damaging	Het
Tnfaip3	T	C	10: 19,008,189	K148E	probably benign	Het
Trrap	T	G	5: 144,816,053	I1813R	probably damaging	Het
Ttc29	A	G	8: 78,251,732	E137G	probably benign	Het
Tubgcp6	C	A	15: 89,116,241	R307L	possibly damaging	Het
Tyro3	T	C	2: 119,801,695	I81T	possibly damaging	Het
Upp2	T	G	2: 58,771,452	M71R	probably damaging	Het
Utp20	G	T	10: 88,753,026	T2427K	probably benign	Het
Vcl	T	A	14: 20,982,699	L122Q	probably damaging	Het
Vmn1r213	A	C	13: 23,012,306	N353T	possibly damaging	Het
Vwa5b2	G	T	16: 20,604,832	S1165I	possibly damaging	Het
Zfp869	T	C	8: 69,707,438	K162E	probably benign	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52121007	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52111070	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52119117	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52085140	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52095948	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52111303	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52111522	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52112543	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52105515	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52116696	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52077065	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52093576	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52110125	nonsense	probably null
IGL03060:Rif1	APN	2	52112137	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52103622	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52090261	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52076988	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52112599	missense	probably benign	0.32
hifi	UTSW	2	52110324	unclassified	probably benign
nietzsche	UTSW	2	52077020	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52111958	missense
R0017:Rif1	UTSW	2	52116674	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52116674	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52111117	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52111117	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52110092	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52090286	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52110324	unclassified	probably benign
R0278:Rif1	UTSW	2	52110324	unclassified	probably benign
R0288:Rif1	UTSW	2	52110013	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52110324	unclassified	probably benign
R0345:Rif1	UTSW	2	52110324	unclassified	probably benign
R0346:Rif1	UTSW	2	52110324	unclassified	probably benign
R0383:Rif1	UTSW	2	52085141	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52110324	unclassified	probably benign
R0387:Rif1	UTSW	2	52110324	unclassified	probably benign
R0388:Rif1	UTSW	2	52110324	unclassified	probably benign
R0456:Rif1	UTSW	2	52110324	unclassified	probably benign
R0477:Rif1	UTSW	2	52110324	unclassified	probably benign
R0505:Rif1	UTSW	2	52110737	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52110324	unclassified	probably benign
R0511:Rif1	UTSW	2	52110324	unclassified	probably benign
R0512:Rif1	UTSW	2	52110324	unclassified	probably benign
R0633:Rif1	UTSW	2	52112563	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52110324	unclassified	probably benign
R0638:Rif1	UTSW	2	52111588	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52110324	unclassified	probably benign
R0675:Rif1	UTSW	2	52110324	unclassified	probably benign
R0707:Rif1	UTSW	2	52110324	unclassified	probably benign
R0726:Rif1	UTSW	2	52110353	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52110324	unclassified	probably benign
R0744:Rif1	UTSW	2	52110324	unclassified	probably benign
R0938:Rif1	UTSW	2	52110324	unclassified	probably benign
R0939:Rif1	UTSW	2	52110324	unclassified	probably benign
R0940:Rif1	UTSW	2	52110324	unclassified	probably benign
R0941:Rif1	UTSW	2	52110324	unclassified	probably benign
R0942:Rif1	UTSW	2	52110324	unclassified	probably benign
R0943:Rif1	UTSW	2	52110324	unclassified	probably benign
R1006:Rif1	UTSW	2	52085029	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52111562	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52110324	unclassified	probably benign
R1175:Rif1	UTSW	2	52107628	unclassified	probably benign
R1183:Rif1	UTSW	2	52110324	unclassified	probably benign
R1184:Rif1	UTSW	2	52110324	unclassified	probably benign
R1271:Rif1	UTSW	2	52110324	unclassified	probably benign
R1332:Rif1	UTSW	2	52078314	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52078314	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52111555	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52110324	unclassified	probably benign
R1527:Rif1	UTSW	2	52110324	unclassified	probably benign
R1560:Rif1	UTSW	2	52111131	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52073223	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52110324	unclassified	probably benign
R1625:Rif1	UTSW	2	52103640	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52110324	unclassified	probably benign
R1689:Rif1	UTSW	2	52110324	unclassified	probably benign
R1731:Rif1	UTSW	2	52110324	unclassified	probably benign
R1744:Rif1	UTSW	2	52112392	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52110324	unclassified	probably benign
R1748:Rif1	UTSW	2	52110324	unclassified	probably benign
R1831:Rif1	UTSW	2	52078495	nonsense	probably null
R1964:Rif1	UTSW	2	52098409	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52110324	unclassified	probably benign
R2000:Rif1	UTSW	2	52081298	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52092346	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52093576	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52110324	unclassified	probably benign
R2109:Rif1	UTSW	2	52110324	unclassified	probably benign
R2125:Rif1	UTSW	2	52110324	unclassified	probably benign
R2126:Rif1	UTSW	2	52110324	unclassified	probably benign
R2145:Rif1	UTSW	2	52111400	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52110324	unclassified	probably benign
R2153:Rif1	UTSW	2	52110324	unclassified	probably benign
R2213:Rif1	UTSW	2	52110324	unclassified	probably benign
R2327:Rif1	UTSW	2	52110324	unclassified	probably benign
R2512:Rif1	UTSW	2	52110324	unclassified	probably benign
R2513:Rif1	UTSW	2	52110324	unclassified	probably benign
R2516:Rif1	UTSW	2	52110324	unclassified	probably benign
R2520:Rif1	UTSW	2	52110324	unclassified	probably benign
R2905:Rif1	UTSW	2	52098504	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52082764	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52110324	unclassified	probably benign
R3156:Rif1	UTSW	2	52110324	unclassified	probably benign
R3429:Rif1	UTSW	2	52110324	unclassified	probably benign
R3707:Rif1	UTSW	2	52093580	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52112545	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52116747	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52121087	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52098471	nonsense	probably null
R4668:Rif1	UTSW	2	52111952	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52106942	missense	probably null	1.00
R4715:Rif1	UTSW	2	52073139	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52098934	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52112747	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52112747	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52093611	intron	probably benign
R4911:Rif1	UTSW	2	52110518	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52084986	splice site	probably null
R5044:Rif1	UTSW	2	52109928	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52092295	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52120309	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52081289	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52077020	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52111824	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52120971	intron	probably benign
R5476:Rif1	UTSW	2	52089595	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52098916	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52121158	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52105639	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52095844	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52085053	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52119156	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52107669	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52078263	splice site	probably null
R6928:Rif1	UTSW	2	52095961	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52112691	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52076989	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52105619	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52078507	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52076175	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52088654	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52085141	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52116356	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52078387	nonsense	probably null
R7962:Rif1	UTSW	2	52074276	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52090278	missense	noncoding transcript
R8390:Rif1	UTSW	2	52110923	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52112551	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52110999	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52111730	missense
R8785:Rif1	UTSW	2	52110481	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52098863	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52110977	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52111850	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52108552	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52111139	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52120344	missense
R9477:Rif1	UTSW	2	52111330	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52081299	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52110454	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52089595	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52074315	missense	probably benign	0.00
X0064:Rif1	UTSW	2	52094633	missense	probably damaging	0.96
Z1177:Rif1	UTSW	2	52088648	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTTCATGGAGAAGGCTAAGGC -3'
(R):5'- CTGGGTAAACACTTTCTGTTGG -3'

Sequencing Primer
(F):5'- GCTAAGGCCAATCAATCAGC -3'
(R):5'- ACAGCATGGATTCTTGGGCC -3'

Posted On

2014-06-30