Mutagenetix > Incidental Mutation

Incidental Mutation 'R0125:Sell'

21256

Institutional Source

Beutler Lab

Gene Symbol

Sell

Ensembl Gene

ENSMUSG00000026581

Gene Name

selectin, lymphocyte

Synonyms

CD62L, Ly-22, Lyam1, LECAM-1, Lyam-1, Ly-m22, Lnhr, L-selectin

MMRRC Submission

038410-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0125 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

163889556-163908354 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 163899674 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000141365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027871] [ENSMUST00000097491] [ENSMUST00000192047] [ENSMUST00000195358]

AlphaFold

P18337

Predicted Effect

probably benign
Transcript: ENSMUST00000027871

SMART Domains

Protein: ENSMUSP00000027871
Gene: ENSMUSG00000026581

Domain	Start	End	E-Value	Type
CLECT	27	156	1.14e-19	SMART
EGF	159	192	6.55e-1	SMART
CCP	197	254	1.09e-11	SMART
CCP	259	316	1.09e-11	SMART
transmembrane domain	333	355	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000097491

SMART Domains

Protein: ENSMUSP00000095099
Gene: ENSMUSG00000026581

Domain	Start	End	E-Value	Type
CLECT	27	156	1.14e-19	SMART
CCP	161	218	1.09e-11	SMART
CCP	223	280	1.09e-11	SMART
transmembrane domain	297	319	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000192047

SMART Domains

Protein: ENSMUSP00000142237
Gene: ENSMUSG00000026581

Domain	Start	End	E-Value	Type
CLECT	27	156	1.14e-19	SMART
CCP	161	218	1.09e-11	SMART
CCP	223	280	1.09e-11	SMART
transmembrane domain	297	319	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000195358

SMART Domains

Protein: ENSMUSP00000141365
Gene: ENSMUSG00000026581

Domain	Start	End	E-Value	Type
Pfam:Sushi	1	31	1.3e-4	PFAM
transmembrane domain	48	70	N/A	INTRINSIC

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 94.7%
20x: 87.1%

Validation Efficiency

98% (96/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
PHENOTYPE: Homozygotes for targeted null mutations exhibit lack of lymphocyte binding to high endothelial venules of peripheral lymph nodes and defects in leukocyte rolling and neutrophil migration into the peritoneum following an inflammatory stimulus. Tumor cellsurvival is also reduced. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	T	C	14: 70,394,096 (GRCm39)		probably benign	Het
Adam23	T	C	1: 63,573,515 (GRCm39)	L261P	probably benign	Het
Adgra3	G	A	5: 50,159,194 (GRCm39)		probably benign	Het
Agtr1b	A	G	3: 20,369,704 (GRCm39)	F301L	probably benign	Het
Ahnak2	G	A	12: 112,748,776 (GRCm39)	T357I	probably benign	Het
Aldh1a7	T	C	19: 20,704,430 (GRCm39)		probably benign	Het
Apoh	A	T	11: 108,302,899 (GRCm39)	N288I	probably damaging	Het
Arfgap3	A	G	15: 83,227,340 (GRCm39)	V24A	probably benign	Het
Atp6v0a1	A	G	11: 100,929,677 (GRCm39)		probably null	Het
Axl	A	T	7: 25,486,368 (GRCm39)	M112K	probably benign	Het
Bnc2	A	C	4: 84,211,169 (GRCm39)	I425S	probably damaging	Het
Ccn4	T	C	15: 66,789,194 (GRCm39)	S227P	possibly damaging	Het
Cdc42bpa	C	T	1: 179,788,763 (GRCm39)	T30M	probably damaging	Het
Cebpz	C	A	17: 79,227,317 (GRCm39)	R1051M	possibly damaging	Het
Ces1d	A	C	8: 93,901,810 (GRCm39)		probably benign	Het
Chd1l	T	C	3: 97,494,465 (GRCm39)	N405S	probably benign	Het
Chodl	G	T	16: 78,738,311 (GRCm39)	G93V	probably damaging	Het
Cpeb2	C	T	5: 43,395,743 (GRCm39)		probably benign	Het
Crebbp	A	G	16: 3,935,105 (GRCm39)		probably benign	Het
Crybb3	T	C	5: 113,227,675 (GRCm39)	T49A	possibly damaging	Het
Ctps1	A	G	4: 120,418,722 (GRCm39)		probably benign	Het
Cyp26b1	A	G	6: 84,551,497 (GRCm39)	Y240H	probably damaging	Het
Cyp2d11	A	C	15: 82,273,422 (GRCm39)	V483G	probably benign	Het
Dennd2b	T	C	7: 109,155,545 (GRCm39)	K402E	probably benign	Het
Dnah14	A	T	1: 181,579,628 (GRCm39)	N3054Y	probably damaging	Het
Dspp	A	C	5: 104,325,905 (GRCm39)	D756A	unknown	Het
Dst	T	C	1: 34,309,984 (GRCm39)	S1553P	probably damaging	Het
Elp4	A	G	2: 105,622,559 (GRCm39)		probably null	Het
Eml6	G	T	11: 29,832,088 (GRCm39)	T194K	probably benign	Het
Evi5	A	G	5: 107,943,638 (GRCm39)	I569T	probably benign	Het
Fancm	C	T	12: 65,168,730 (GRCm39)	P1698S	possibly damaging	Het
Fhdc1	T	C	3: 84,352,852 (GRCm39)	D791G	probably benign	Het
Frem1	A	G	4: 82,930,188 (GRCm39)	Y253H	probably damaging	Het
Gpn3	A	G	5: 122,519,481 (GRCm39)	Y196C	probably benign	Het
Hcls1	A	G	16: 36,782,525 (GRCm39)	D398G	probably benign	Het
Hydin	T	C	8: 111,189,163 (GRCm39)	V1189A	probably benign	Het
Itgb3	G	A	11: 104,534,789 (GRCm39)	D549N	probably damaging	Het
Itpr2	A	G	6: 146,141,951 (GRCm39)	F1697S	probably benign	Het
Klk1b11	A	G	7: 43,648,475 (GRCm39)	T161A	probably benign	Het
Kntc1	G	A	5: 123,903,120 (GRCm39)		probably benign	Het
Map3k19	A	T	1: 127,750,837 (GRCm39)	F838Y	probably benign	Het
Map6	T	A	7: 98,985,187 (GRCm39)		probably null	Het
Mcrs1	A	G	15: 99,142,608 (GRCm39)		probably benign	Het
Mdn1	A	T	4: 32,729,956 (GRCm39)	Y2766F	probably damaging	Het
Med23	C	T	10: 24,776,686 (GRCm39)	H739Y	probably damaging	Het
Mmp17	T	A	5: 129,671,646 (GRCm39)	D65E	possibly damaging	Het
Mmp9	T	A	2: 164,793,177 (GRCm39)	L442Q	probably damaging	Het
Myo19	T	C	11: 84,779,001 (GRCm39)		probably benign	Het
Nedd1	A	C	10: 92,527,791 (GRCm39)	S468A	possibly damaging	Het
Niban2	T	A	2: 32,813,833 (GRCm39)	V682D	probably benign	Het
Nlrp4d	A	C	7: 10,116,316 (GRCm39)	V152G	probably damaging	Het
Nxf1	T	A	19: 8,740,170 (GRCm39)	D112E	probably benign	Het
Oas1h	A	T	5: 121,000,626 (GRCm39)	K79*	probably null	Het
Omg	T	A	11: 79,393,679 (GRCm39)	I60F	possibly damaging	Het
Or5p69	A	G	7: 107,967,576 (GRCm39)	Y293C	probably damaging	Het
Or8b101	A	G	9: 38,020,815 (GRCm39)	T278A	probably benign	Het
Or8b1b	T	A	9: 38,375,757 (GRCm39)	L140*	probably null	Het
Pck1	G	A	2: 172,997,874 (GRCm39)	W314*	probably null	Het
Pla2g15	T	C	8: 106,889,756 (GRCm39)	Y343H	probably benign	Het
Plcb3	T	C	19: 6,936,276 (GRCm39)	E749G	probably damaging	Het
Plgrkt	A	G	19: 29,328,442 (GRCm39)		probably null	Het
Pprc1	A	G	19: 46,057,951 (GRCm39)		probably benign	Het
Prkdc	A	T	16: 15,516,871 (GRCm39)	I1082F	probably damaging	Het
Rapgef6	T	A	11: 54,516,701 (GRCm39)	Y172*	probably null	Het
Ros1	G	T	10: 52,001,885 (GRCm39)	A1079D	probably benign	Het
Sap30	T	C	8: 57,938,545 (GRCm39)	E147G	probably null	Het
Senp1	A	T	15: 97,946,112 (GRCm39)	D544E	probably damaging	Het
Shpk	G	A	11: 73,105,048 (GRCm39)		probably benign	Het
Slc35b1	A	T	11: 95,277,353 (GRCm39)	T74S	probably benign	Het
Slc6a3	T	A	13: 73,718,098 (GRCm39)		probably benign	Het
Slf1	T	C	13: 77,191,864 (GRCm39)	N990S	probably benign	Het
Smgc	A	G	15: 91,738,746 (GRCm39)		probably benign	Het
Snx19	T	A	9: 30,351,515 (GRCm39)	V861D	probably damaging	Het
Sprr2e	C	T	3: 92,260,285 (GRCm39)	P39S	unknown	Het
Sstr2	T	A	11: 113,515,303 (GRCm39)	M74K	probably damaging	Het
Svep1	T	C	4: 58,099,937 (GRCm39)		probably benign	Het
Tas2r143	A	G	6: 42,377,889 (GRCm39)	I240V	probably benign	Het
Tbrg1	T	C	9: 37,563,937 (GRCm39)	I233V	probably benign	Het
Tecpr1	G	T	5: 144,134,717 (GRCm39)	D1055E	probably damaging	Het
Thap2	A	T	10: 115,212,277 (GRCm39)		probably null	Het
Tinagl1	A	G	4: 130,060,101 (GRCm39)	Y388H	probably damaging	Het
Ttn	T	A	2: 76,585,896 (GRCm39)	Y21945F	probably damaging	Het
Ugt2b1	A	T	5: 87,073,961 (GRCm39)	W133R	probably benign	Het
Usp24	C	T	4: 106,254,496 (GRCm39)	P1491L	possibly damaging	Het
Utp15	A	G	13: 98,387,390 (GRCm39)	S395P	possibly damaging	Het
Vav1	T	A	17: 57,606,847 (GRCm39)	L254Q	probably damaging	Het
Vmn2r104	T	C	17: 20,250,069 (GRCm39)	Y734C	probably damaging	Het
Vps8	T	A	16: 21,288,904 (GRCm39)	V421E	probably benign	Het
Xkr7	G	T	2: 152,874,346 (GRCm39)	A138S	probably benign	Het

Other mutations in Sell

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01981:Sell	APN	1	163,893,195 (GRCm39)	missense	probably benign	0.04
IGL02466:Sell	APN	1	163,896,632 (GRCm39)	splice site	probably null
IGL02578:Sell	APN	1	163,893,165 (GRCm39)	missense	probably damaging	1.00
IGL03243:Sell	APN	1	163,892,911 (GRCm39)	missense	possibly damaging	0.94
dim_sum	UTSW	1	163,893,770 (GRCm39)	splice site	probably null
dim_sum2	UTSW	1	163,893,230 (GRCm39)	nonsense	probably null
Duct	UTSW	1	163,893,122 (GRCm39)	missense	probably damaging	1.00
Postit	UTSW	1	163,893,176 (GRCm39)	missense	possibly damaging	0.85
R0800:Sell	UTSW	1	163,893,770 (GRCm39)	splice site	probably null
R1900:Sell	UTSW	1	163,892,907 (GRCm39)	missense	probably damaging	1.00
R3848:Sell	UTSW	1	163,893,230 (GRCm39)	nonsense	probably null
R4553:Sell	UTSW	1	163,899,685 (GRCm39)	missense	probably benign	0.08
R4671:Sell	UTSW	1	163,893,042 (GRCm39)	missense	probably damaging	1.00
R4685:Sell	UTSW	1	163,893,829 (GRCm39)	missense	probably damaging	1.00
R4896:Sell	UTSW	1	163,890,631 (GRCm39)	missense	probably benign	0.02
R4970:Sell	UTSW	1	163,892,887 (GRCm39)	missense	possibly damaging	0.75
R5112:Sell	UTSW	1	163,892,887 (GRCm39)	missense	possibly damaging	0.75
R6549:Sell	UTSW	1	163,893,198 (GRCm39)	missense	probably damaging	1.00
R7148:Sell	UTSW	1	163,893,176 (GRCm39)	missense	possibly damaging	0.85
R7545:Sell	UTSW	1	163,892,903 (GRCm39)	missense	probably benign	0.21
R8010:Sell	UTSW	1	163,893,081 (GRCm39)	missense	possibly damaging	0.92
R9026:Sell	UTSW	1	163,893,042 (GRCm39)	missense	probably damaging	1.00
R9239:Sell	UTSW	1	163,893,176 (GRCm39)	missense	possibly damaging	0.85
R9329:Sell	UTSW	1	163,893,122 (GRCm39)	missense	probably damaging	1.00
R9336:Sell	UTSW	1	163,893,177 (GRCm39)	missense	probably damaging	1.00
R9455:Sell	UTSW	1	163,894,218 (GRCm39)	missense	probably benign	0.02
R9699:Sell	UTSW	1	163,893,114 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTGAGATACATCCCCAGGACCTCC -3'
(R):5'- AGTCACTTGGCACCTGCTTCAAC -3'

Sequencing Primer
(F):5'- AGATCTGAGAACTGTGGTCCC -3'
(R):5'- ctctctctctctctctctctctc -3'

Posted On

2013-04-11