Mutagenetix > Incidental Mutation

Incidental Mutation 'R1917:Ep400'

212597

Institutional Source

Beutler Lab

Gene Symbol

Ep400

Ensembl Gene

ENSMUSG00000029505

Gene Name

E1A binding protein p400

Synonyms

mDomino, 1700020J09Rik, p400

MMRRC Submission

039935-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1917 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

110664373-110770717 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 110703575 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 1347 (K1347R)

Ref Sequence

ENSEMBL: ENSMUSP00000108055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041558] [ENSMUST00000112435] [ENSMUST00000112436]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000041558
AA Change: K1383R

SMART Domains

Protein: ENSMUSP00000049038
Gene: ENSMUSG00000029505
AA Change: K1383R

Domain	Start	End	E-Value	Type
Pfam:EP400_N	1	461	1.6e-232	PFAM
low complexity region	519	532	N/A	INTRINSIC
low complexity region	550	561	N/A	INTRINSIC
low complexity region	598	620	N/A	INTRINSIC
low complexity region	631	645	N/A	INTRINSIC
low complexity region	658	686	N/A	INTRINSIC
HSA	762	833	1.31e-31	SMART
low complexity region	908	925	N/A	INTRINSIC
DEXDc	1049	1238	2.76e-15	SMART
Blast:DEXDc	1276	1317	2e-15	BLAST
low complexity region	1407	1417	N/A	INTRINSIC
HELICc	1807	1893	1.17e-4	SMART
low complexity region	2006	2019	N/A	INTRINSIC
low complexity region	2080	2100	N/A	INTRINSIC
low complexity region	2214	2223	N/A	INTRINSIC
SANT	2243	2310	3.57e-1	SMART
low complexity region	2402	2489	N/A	INTRINSIC
low complexity region	2596	2608	N/A	INTRINSIC
low complexity region	2644	2679	N/A	INTRINSIC
low complexity region	2694	2738	N/A	INTRINSIC
low complexity region	2769	2806	N/A	INTRINSIC
low complexity region	2846	2883	N/A	INTRINSIC
low complexity region	2933	2947	N/A	INTRINSIC
low complexity region	2974	2986	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000112435
AA Change: K1420R

SMART Domains

Protein: ENSMUSP00000108054
Gene: ENSMUSG00000029505
AA Change: K1420R

Domain	Start	End	E-Value	Type
low complexity region	28	53	N/A	INTRINSIC
low complexity region	121	145	N/A	INTRINSIC
low complexity region	262	287	N/A	INTRINSIC
low complexity region	298	308	N/A	INTRINSIC
low complexity region	313	329	N/A	INTRINSIC
coiled coil region	418	447	N/A	INTRINSIC
low complexity region	471	485	N/A	INTRINSIC
low complexity region	556	569	N/A	INTRINSIC
low complexity region	587	598	N/A	INTRINSIC
low complexity region	635	657	N/A	INTRINSIC
low complexity region	668	682	N/A	INTRINSIC
low complexity region	695	723	N/A	INTRINSIC
HSA	799	870	1.31e-31	SMART
low complexity region	945	962	N/A	INTRINSIC
DEXDc	1086	1275	2.76e-15	SMART
Blast:DEXDc	1313	1354	2e-15	BLAST
low complexity region	1444	1454	N/A	INTRINSIC
internal_repeat_1	1556	1646	6.82e-5	PROSPERO
low complexity region	1887	1900	N/A	INTRINSIC
low complexity region	1961	1981	N/A	INTRINSIC
low complexity region	2095	2104	N/A	INTRINSIC
SANT	2124	2191	3.57e-1	SMART
low complexity region	2283	2370	N/A	INTRINSIC
internal_repeat_1	2371	2463	6.82e-5	PROSPERO
low complexity region	2477	2489	N/A	INTRINSIC
low complexity region	2525	2560	N/A	INTRINSIC
low complexity region	2575	2619	N/A	INTRINSIC
low complexity region	2645	2659	N/A	INTRINSIC
low complexity region	2660	2680	N/A	INTRINSIC
low complexity region	2720	2757	N/A	INTRINSIC
low complexity region	2807	2821	N/A	INTRINSIC
low complexity region	2848	2860	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000112436
AA Change: K1347R

SMART Domains

Protein: ENSMUSP00000108055
Gene: ENSMUSG00000029505
AA Change: K1347R

Domain	Start	End	E-Value	Type
low complexity region	28	53	N/A	INTRINSIC
low complexity region	121	145	N/A	INTRINSIC
low complexity region	262	287	N/A	INTRINSIC
low complexity region	298	308	N/A	INTRINSIC
low complexity region	313	329	N/A	INTRINSIC
coiled coil region	418	449	N/A	INTRINSIC
low complexity region	472	482	N/A	INTRINSIC
low complexity region	483	496	N/A	INTRINSIC
low complexity region	514	525	N/A	INTRINSIC
low complexity region	562	584	N/A	INTRINSIC
low complexity region	595	609	N/A	INTRINSIC
low complexity region	622	650	N/A	INTRINSIC
HSA	726	797	1.31e-31	SMART
low complexity region	872	889	N/A	INTRINSIC
DEXDc	1013	1202	2.76e-15	SMART
Blast:DEXDc	1240	1281	2e-15	BLAST
low complexity region	1371	1381	N/A	INTRINSIC
internal_repeat_1	1483	1573	6.76e-5	PROSPERO
HELICc	1771	1857	1.17e-4	SMART
low complexity region	1970	1983	N/A	INTRINSIC
low complexity region	2044	2064	N/A	INTRINSIC
low complexity region	2178	2187	N/A	INTRINSIC
SANT	2207	2274	3.57e-1	SMART
low complexity region	2366	2453	N/A	INTRINSIC
internal_repeat_1	2454	2546	6.76e-5	PROSPERO
low complexity region	2560	2572	N/A	INTRINSIC
low complexity region	2608	2643	N/A	INTRINSIC
low complexity region	2658	2702	N/A	INTRINSIC
low complexity region	2733	2770	N/A	INTRINSIC
low complexity region	2810	2847	N/A	INTRINSIC
low complexity region	2897	2911	N/A	INTRINSIC
low complexity region	2938	2950	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000125325
AA Change: K82R

SMART Domains

Protein: ENSMUSP00000116137
Gene: ENSMUSG00000029505
AA Change: K82R

Domain	Start	End	E-Value	Type
low complexity region	107	117	N/A	INTRINSIC
low complexity region	223	239	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141986

Meta Mutation Damage Score

0.0581

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.8%
20x: 93.6%

Validation Efficiency

100% (83/83)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele die at E11.5 and display severe defects in yolk sac erythropoiesis, anemia, and a slight deformity of the neural tube. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931440F15Rik	A	G	11: 29,824,039 (GRCm38)	S473P	probably benign	Het
Abca8a	A	G	11: 110,091,515 (GRCm38)		probably benign	Het
Adgre5	A	G	8: 83,729,109 (GRCm38)	V190A	probably damaging	Het
Akap6	A	G	12: 53,104,612 (GRCm38)	N1153S	probably benign	Het
Aldh1a7	G	A	19: 20,727,455 (GRCm38)	H20Y	probably benign	Het
B430306N03Rik	A	G	17: 48,324,148 (GRCm38)	E278G	probably benign	Het
Cacna1b	C	A	2: 24,616,879 (GRCm38)	R72L	probably null	Het
Cc2d2a	C	G	5: 43,706,222 (GRCm38)	S675R	probably damaging	Het
Ccdc88b	T	A	19: 6,849,226 (GRCm38)	E1040D	probably damaging	Het
Cmtm7	A	G	9: 114,763,364 (GRCm38)	V55A	probably damaging	Het
Coq4	A	C	2: 29,789,926 (GRCm38)	T77P	probably damaging	Het
Cyp2j11	C	A	4: 96,339,974 (GRCm38)	W136L	probably damaging	Het
Dctn2	T	A	10: 127,275,049 (GRCm38)	Y86*	probably null	Het
Ddx56	A	G	11: 6,263,937 (GRCm38)		probably null	Het
Dopey2	T	C	16: 93,716,262 (GRCm38)	S30P	probably damaging	Het
Fat3	T	A	9: 15,997,057 (GRCm38)	T2550S	possibly damaging	Het
Fcrla	G	T	1: 170,927,526 (GRCm38)	C5*	probably null	Het
Fermt1	T	A	2: 132,922,842 (GRCm38)	D365V	probably damaging	Het
Fhod3	A	G	18: 24,989,965 (GRCm38)		probably benign	Het
Fhod3	A	G	18: 25,085,601 (GRCm38)	D807G	probably benign	Het
Fnip1	C	T	11: 54,480,684 (GRCm38)	T177I	probably damaging	Het
Gart	T	C	16: 91,628,149 (GRCm38)	Y662C	probably damaging	Het
Gda	A	T	19: 21,397,640 (GRCm38)		probably benign	Het
Gk	A	G	X: 85,760,580 (GRCm38)	I85T	probably damaging	Het
Gm12169	A	G	11: 46,528,531 (GRCm38)	D58G	possibly damaging	Het
Gm14548	C	T	7: 3,897,638 (GRCm38)	V38M	probably damaging	Het
Gm3476	A	G	14: 6,118,358 (GRCm38)	L255P	possibly damaging	Het
Gm9966	T	C	7: 95,958,477 (GRCm38)	C2R	unknown	Het
Gnpda1	T	C	18: 38,333,190 (GRCm38)		probably null	Het
Gtf2h4	G	A	17: 35,670,198 (GRCm38)	L246F	possibly damaging	Het
Hao1	A	T	2: 134,523,060 (GRCm38)	S216T	probably benign	Het
Hnrnpr	C	A	4: 136,332,488 (GRCm38)	S301*	probably null	Het
Hsd3b2	A	G	3: 98,712,026 (GRCm38)	I201T	probably benign	Het
Jade2	T	C	11: 51,818,538 (GRCm38)	E548G	possibly damaging	Het
Katnbl1	T	C	2: 112,409,179 (GRCm38)	I241T	probably benign	Het
Keap1	G	T	9: 21,233,806 (GRCm38)	Q299K	probably benign	Het
Kif1a	T	C	1: 93,019,031 (GRCm38)	I1650V	possibly damaging	Het
Lrba	G	A	3: 86,664,501 (GRCm38)	G275R	probably damaging	Het
Map3k9	C	A	12: 81,780,790 (GRCm38)	E29*	probably null	Het
Mat1a	G	A	14: 41,121,437 (GRCm38)	V307I	probably damaging	Het
Mcm2	A	T	6: 88,891,803 (GRCm38)	M324K	possibly damaging	Het
Metap1d	T	C	2: 71,511,527 (GRCm38)	V155A	probably damaging	Het
Mtbp	A	G	15: 55,564,677 (GRCm38)		probably benign	Het
Myh14	T	C	7: 44,657,925 (GRCm38)	T231A	probably benign	Het
Mylk4	A	T	13: 32,724,853 (GRCm38)	D90E	probably benign	Het
Myo15b	T	A	11: 115,882,254 (GRCm38)	I1837K	possibly damaging	Het
Myo3a	T	A	2: 22,291,922 (GRCm38)	H242Q	probably damaging	Het
Nxn	A	G	11: 76,261,672 (GRCm38)		probably benign	Het
Olfr791	T	A	10: 129,527,049 (GRCm38)	V274D	probably damaging	Het
Pak3	C	A	X: 143,791,302 (GRCm38)	A553E	possibly damaging	Het
Pdia2	T	A	17: 26,198,105 (GRCm38)	T122S	possibly damaging	Het
Plod2	A	G	9: 92,581,257 (GRCm38)	T132A	probably benign	Het
Ptprz1	T	A	6: 23,035,040 (GRCm38)		probably benign	Het
Rad54b	A	C	4: 11,601,693 (GRCm38)	N416T	probably damaging	Het
Recql4	A	T	15: 76,703,837 (GRCm38)	Y1142*	probably null	Het
Rnf216	A	G	5: 142,992,806 (GRCm38)	V859A	probably benign	Het
Scnm1	G	T	3: 95,130,273 (GRCm38)	P161T	possibly damaging	Het
Serpinb6b	A	G	13: 32,978,240 (GRCm38)	I222V	probably benign	Het
Serpinf1	A	G	11: 75,411,007 (GRCm38)	I274T	possibly damaging	Het
Slc28a1	T	C	7: 81,169,586 (GRCm38)	F641L	probably benign	Het
Slc8a2	A	G	7: 16,152,920 (GRCm38)	I657V	probably benign	Het
Smchd1	A	G	17: 71,407,237 (GRCm38)	I877T	possibly damaging	Het
Spata31	T	C	13: 64,920,865 (GRCm38)	Y276H	possibly damaging	Het
Spire2	A	G	8: 123,363,071 (GRCm38)	D447G	probably benign	Het
Stk3	G	A	15: 35,073,217 (GRCm38)	T119I	probably damaging	Het
Stxbp5	C	A	10: 9,812,298 (GRCm38)	V420F	possibly damaging	Het
Sult2a5	T	C	7: 13,670,684 (GRCm38)	F282S	probably damaging	Het
Syk	A	T	13: 52,622,708 (GRCm38)	D248V	probably damaging	Het
Thoc6	C	T	17: 23,669,390 (GRCm38)		probably benign	Het
Tll2	T	A	19: 41,128,497 (GRCm38)	D293V	possibly damaging	Het
Umodl1	G	A	17: 30,984,043 (GRCm38)	V457M	probably damaging	Het
Usp19	T	A	9: 108,499,325 (GRCm38)	C689*	probably null	Het
Usp24	T	G	4: 106,410,286 (GRCm38)	V1955G	probably damaging	Het
Vmn1r226	T	C	17: 20,687,580 (GRCm38)	S25P	probably damaging	Het
Vmn2r69	C	T	7: 85,411,683 (GRCm38)	C231Y	probably damaging	Het
Wdr73	C	T	7: 80,893,333 (GRCm38)	D176N	probably benign	Het
Wnt7b	T	A	15: 85,559,080 (GRCm38)	I41F	probably damaging	Het
Zfhx3	T	C	8: 108,956,248 (GRCm38)	S3440P	unknown	Het
Zfp52	T	G	17: 21,560,164 (GRCm38)	N91K	probably benign	Het
Zfp930	C	A	8: 69,228,705 (GRCm38)	Q350K	probably benign	Het
Zfp949	T	C	9: 88,570,062 (GRCm38)	S562P	probably damaging	Het

Other mutations in Ep400

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Ep400	APN	5	110,687,841 (GRCm38)	missense	unknown
IGL00585:Ep400	APN	5	110,755,905 (GRCm38)	missense	possibly damaging	0.70
IGL00586:Ep400	APN	5	110,739,594 (GRCm38)	missense	probably damaging	1.00
IGL00816:Ep400	APN	5	110,735,490 (GRCm38)	unclassified	probably benign
IGL01066:Ep400	APN	5	110,668,199 (GRCm38)	splice site	probably benign
IGL01302:Ep400	APN	5	110,742,048 (GRCm38)	missense	probably benign	0.00
IGL01568:Ep400	APN	5	110,719,495 (GRCm38)	missense	unknown
IGL01833:Ep400	APN	5	110,680,008 (GRCm38)	missense	unknown
IGL02086:Ep400	APN	5	110,676,943 (GRCm38)	splice site	probably benign
IGL02266:Ep400	APN	5	110,695,297 (GRCm38)	unclassified	probably benign
IGL02288:Ep400	APN	5	110,683,836 (GRCm38)	splice site	probably benign
IGL02301:Ep400	APN	5	110,674,960 (GRCm38)	missense	probably damaging	1.00
IGL02377:Ep400	APN	5	110,720,825 (GRCm38)	missense	unknown
IGL02382:Ep400	APN	5	110,701,728 (GRCm38)	missense	unknown
IGL02419:Ep400	APN	5	110,697,376 (GRCm38)	splice site	probably null
IGL02591:Ep400	APN	5	110,733,772 (GRCm38)	unclassified	probably benign
IGL02981:Ep400	APN	5	110,691,610 (GRCm38)	splice site	probably benign
IGL02981:Ep400	APN	5	110,756,103 (GRCm38)	missense	possibly damaging	0.79
IGL03173:Ep400	APN	5	110,708,871 (GRCm38)	unclassified	probably benign
IGL03244:Ep400	APN	5	110,727,563 (GRCm38)	missense	unknown
IGL03333:Ep400	APN	5	110,703,566 (GRCm38)	missense	unknown
santol	UTSW	5	110,701,671 (GRCm38)	missense	unknown
PIT4243001:Ep400	UTSW	5	110,735,580 (GRCm38)	missense	unknown
PIT4260001:Ep400	UTSW	5	110,693,171 (GRCm38)	nonsense	probably null
R0017:Ep400	UTSW	5	110,673,529 (GRCm38)	missense	probably damaging	1.00
R0179:Ep400	UTSW	5	110,668,649 (GRCm38)	missense	probably damaging	0.99
R0243:Ep400	UTSW	5	110,724,407 (GRCm38)	splice site	probably benign
R0366:Ep400	UTSW	5	110,701,671 (GRCm38)	missense	unknown
R0508:Ep400	UTSW	5	110,739,508 (GRCm38)	missense	probably benign	0.00
R0541:Ep400	UTSW	5	110,705,016 (GRCm38)	missense	unknown
R0558:Ep400	UTSW	5	110,685,067 (GRCm38)	splice site	probably benign
R0576:Ep400	UTSW	5	110,711,093 (GRCm38)	unclassified	probably benign
R0595:Ep400	UTSW	5	110,703,542 (GRCm38)	missense	unknown
R0671:Ep400	UTSW	5	110,688,196 (GRCm38)	missense	unknown
R0763:Ep400	UTSW	5	110,665,837 (GRCm38)	missense	probably damaging	1.00
R1078:Ep400	UTSW	5	110,735,522 (GRCm38)	unclassified	probably benign
R1300:Ep400	UTSW	5	110,673,560 (GRCm38)	missense	probably damaging	1.00
R1439:Ep400	UTSW	5	110,685,478 (GRCm38)	missense	unknown
R1520:Ep400	UTSW	5	110,691,778 (GRCm38)	intron	probably benign
R1529:Ep400	UTSW	5	110,739,445 (GRCm38)	missense	probably benign	0.00
R1535:Ep400	UTSW	5	110,708,166 (GRCm38)	unclassified	probably benign
R1560:Ep400	UTSW	5	110,671,106 (GRCm38)	splice site	probably null
R1587:Ep400	UTSW	5	110,726,902 (GRCm38)	missense	probably benign	0.23
R1596:Ep400	UTSW	5	110,708,861 (GRCm38)	unclassified	probably benign
R1653:Ep400	UTSW	5	110,693,174 (GRCm38)	nonsense	probably null
R1711:Ep400	UTSW	5	110,693,308 (GRCm38)	unclassified	probably benign
R1774:Ep400	UTSW	5	110,685,491 (GRCm38)	missense	unknown
R1836:Ep400	UTSW	5	110,705,054 (GRCm38)	missense	unknown
R1905:Ep400	UTSW	5	110,670,948 (GRCm38)	missense	probably damaging	1.00
R2064:Ep400	UTSW	5	110,735,404 (GRCm38)	unclassified	probably benign
R2122:Ep400	UTSW	5	110,708,850 (GRCm38)	unclassified	probably benign
R2144:Ep400	UTSW	5	110,703,518 (GRCm38)	missense	unknown
R2215:Ep400	UTSW	5	110,693,555 (GRCm38)	unclassified	probably benign
R2252:Ep400	UTSW	5	110,719,091 (GRCm38)	missense	unknown
R2253:Ep400	UTSW	5	110,719,091 (GRCm38)	missense	unknown
R2483:Ep400	UTSW	5	110,719,236 (GRCm38)	missense	unknown
R2504:Ep400	UTSW	5	110,668,645 (GRCm38)	missense	probably damaging	1.00
R2512:Ep400	UTSW	5	110,708,915 (GRCm38)	unclassified	probably benign
R2842:Ep400	UTSW	5	110,698,815 (GRCm38)	nonsense	probably null
R2920:Ep400	UTSW	5	110,755,914 (GRCm38)	missense	probably damaging	1.00
R3082:Ep400	UTSW	5	110,693,230 (GRCm38)	unclassified	probably benign
R3151:Ep400	UTSW	5	110,703,569 (GRCm38)	missense	unknown
R3552:Ep400	UTSW	5	110,729,287 (GRCm38)	missense	unknown
R3623:Ep400	UTSW	5	110,719,236 (GRCm38)	missense	unknown
R3779:Ep400	UTSW	5	110,691,649 (GRCm38)	missense	unknown
R3923:Ep400	UTSW	5	110,756,523 (GRCm38)	missense	possibly damaging	0.55
R4062:Ep400	UTSW	5	110,741,981 (GRCm38)	missense	probably benign	0.10
R4508:Ep400	UTSW	5	110,703,615 (GRCm38)	missense	unknown
R4584:Ep400	UTSW	5	110,733,897 (GRCm38)	unclassified	probably benign
R4585:Ep400	UTSW	5	110,753,859 (GRCm38)	missense	probably damaging	1.00
R4586:Ep400	UTSW	5	110,753,859 (GRCm38)	missense	probably damaging	1.00
R4807:Ep400	UTSW	5	110,695,578 (GRCm38)	splice site	probably null
R4921:Ep400	UTSW	5	110,665,810 (GRCm38)	missense	probably damaging	1.00
R4976:Ep400	UTSW	5	110,720,756 (GRCm38)	missense	unknown
R4976:Ep400	UTSW	5	110,698,812 (GRCm38)	missense	unknown
R5075:Ep400	UTSW	5	110,685,485 (GRCm38)	missense	unknown
R5120:Ep400	UTSW	5	110,756,358 (GRCm38)	missense	probably damaging	1.00
R5122:Ep400	UTSW	5	110,668,170 (GRCm38)	missense	probably damaging	1.00
R5223:Ep400	UTSW	5	110,668,630 (GRCm38)	missense	probably damaging	1.00
R5284:Ep400	UTSW	5	110,668,124 (GRCm38)	missense	probably damaging	1.00
R5388:Ep400	UTSW	5	110,701,728 (GRCm38)	missense	unknown
R5401:Ep400	UTSW	5	110,683,171 (GRCm38)	missense	unknown
R5431:Ep400	UTSW	5	110,676,554 (GRCm38)	missense	unknown
R5461:Ep400	UTSW	5	110,676,684 (GRCm38)	nonsense	probably null
R5568:Ep400	UTSW	5	110,756,205 (GRCm38)	missense	probably damaging	1.00
R5650:Ep400	UTSW	5	110,695,952 (GRCm38)	critical splice donor site	probably null
R5778:Ep400	UTSW	5	110,719,584 (GRCm38)	missense	unknown
R5806:Ep400	UTSW	5	110,755,554 (GRCm38)	nonsense	probably null
R5814:Ep400	UTSW	5	110,695,578 (GRCm38)	splice site	probably null
R5830:Ep400	UTSW	5	110,683,996 (GRCm38)	missense	unknown
R5882:Ep400	UTSW	5	110,755,587 (GRCm38)	missense	probably benign	0.00
R5931:Ep400	UTSW	5	110,735,520 (GRCm38)	unclassified	probably benign
R5945:Ep400	UTSW	5	110,682,866 (GRCm38)	missense	unknown
R5966:Ep400	UTSW	5	110,676,900 (GRCm38)	missense	unknown
R5973:Ep400	UTSW	5	110,729,831 (GRCm38)	missense	unknown
R5980:Ep400	UTSW	5	110,733,729 (GRCm38)	unclassified	probably benign
R6000:Ep400	UTSW	5	110,683,201 (GRCm38)	missense	unknown
R6006:Ep400	UTSW	5	110,704,959 (GRCm38)	missense	unknown
R6053:Ep400	UTSW	5	110,755,795 (GRCm38)	missense	probably benign	0.22
R6145:Ep400	UTSW	5	110,756,703 (GRCm38)	missense	possibly damaging	0.95
R6154:Ep400	UTSW	5	110,755,933 (GRCm38)	missense	probably damaging	0.97
R6169:Ep400	UTSW	5	110,741,997 (GRCm38)	missense	possibly damaging	0.83
R6228:Ep400	UTSW	5	110,670,942 (GRCm38)	missense	probably damaging	1.00
R6295:Ep400	UTSW	5	110,753,809 (GRCm38)	missense	probably benign	0.00
R6486:Ep400	UTSW	5	110,697,218 (GRCm38)	unclassified	probably benign
R6504:Ep400	UTSW	5	110,708,837 (GRCm38)	unclassified	probably benign
R6607:Ep400	UTSW	5	110,683,314 (GRCm38)	missense	unknown
R6657:Ep400	UTSW	5	110,693,545 (GRCm38)	unclassified	probably benign
R6660:Ep400	UTSW	5	110,719,447 (GRCm38)	nonsense	probably null
R6741:Ep400	UTSW	5	110,676,895 (GRCm38)	missense	unknown
R6933:Ep400	UTSW	5	110,665,862 (GRCm38)	missense	probably damaging	1.00
R6937:Ep400	UTSW	5	110,711,152 (GRCm38)	unclassified	probably benign
R7069:Ep400	UTSW	5	110,668,124 (GRCm38)	missense	probably damaging	1.00
R7103:Ep400	UTSW	5	110,733,785 (GRCm38)	missense	unknown
R7156:Ep400	UTSW	5	110,685,363 (GRCm38)	missense	unknown
R7272:Ep400	UTSW	5	110,755,645 (GRCm38)	nonsense	probably null
R7365:Ep400	UTSW	5	110,719,614 (GRCm38)	missense	unknown
R7581:Ep400	UTSW	5	110,756,025 (GRCm38)	missense	unknown
R7684:Ep400	UTSW	5	110,697,352 (GRCm38)	missense	unknown
R7699:Ep400	UTSW	5	110,696,032 (GRCm38)	missense	unknown
R7700:Ep400	UTSW	5	110,696,032 (GRCm38)	missense	unknown
R7856:Ep400	UTSW	5	110,666,584 (GRCm38)	missense	probably damaging	0.99
R7954:Ep400	UTSW	5	110,668,733 (GRCm38)	missense	possibly damaging	0.46
R8098:Ep400	UTSW	5	110,693,251 (GRCm38)	missense	unknown
R8108:Ep400	UTSW	5	110,687,883 (GRCm38)	missense	unknown
R8260:Ep400	UTSW	5	110,755,612 (GRCm38)	nonsense	probably null
R8293:Ep400	UTSW	5	110,708,892 (GRCm38)	missense	unknown
R8314:Ep400	UTSW	5	110,755,753 (GRCm38)	missense	unknown
R8351:Ep400	UTSW	5	110,739,334 (GRCm38)	missense	probably damaging	1.00
R8424:Ep400	UTSW	5	110,693,278 (GRCm38)	missense	unknown
R8459:Ep400	UTSW	5	110,708,891 (GRCm38)	missense	unknown
R8529:Ep400	UTSW	5	110,719,236 (GRCm38)	missense	unknown
R8688:Ep400	UTSW	5	110,720,819 (GRCm38)	missense	unknown
R8744:Ep400	UTSW	5	110,742,059 (GRCm38)	missense	unknown
R8923:Ep400	UTSW	5	110,683,998 (GRCm38)	missense	unknown
R9005:Ep400	UTSW	5	110,711,093 (GRCm38)	missense	unknown
R9087:Ep400	UTSW	5	110,667,564 (GRCm38)	nonsense	probably null
R9146:Ep400	UTSW	5	110,701,769 (GRCm38)	nonsense	probably null
R9383:Ep400	UTSW	5	110,685,485 (GRCm38)	missense	unknown
R9479:Ep400	UTSW	5	110,729,864 (GRCm38)	missense	unknown
R9496:Ep400	UTSW	5	110,707,987 (GRCm38)	missense	unknown
R9582:Ep400	UTSW	5	110,676,449 (GRCm38)	critical splice donor site	probably null
R9607:Ep400	UTSW	5	110,683,939 (GRCm38)	missense	unknown
R9712:Ep400	UTSW	5	110,756,643 (GRCm38)	missense	unknown
R9746:Ep400	UTSW	5	110,742,006 (GRCm38)	missense	unknown
X0012:Ep400	UTSW	5	110,673,196 (GRCm38)	small deletion	probably benign
X0021:Ep400	UTSW	5	110,682,864 (GRCm38)	missense	unknown
Z1176:Ep400	UTSW	5	110,756,635 (GRCm38)	missense	unknown
Z1177:Ep400	UTSW	5	110,733,743 (GRCm38)	missense	unknown
Z1177:Ep400	UTSW	5	110,683,364 (GRCm38)	missense	unknown
Z1188:Ep400	UTSW	5	110,755,683 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCATAGCCCAGGCATCTGAC -3'
(R):5'- AGCATCAGAAGGCTTACCAC -3'

Sequencing Primer
(F):5'- ACAGTCCTTGGAGCAGTGACTATG -3'
(R):5'- CACCATATAAAACAGCCTCTTCAG -3'

Posted On

2014-07-14