Mutagenetix > Incidental Mutation

Incidental Mutation 'R1918:Nfat5'

212725

Institutional Source

Beutler Lab

Gene Symbol

Nfat5

Ensembl Gene

ENSMUSG00000003847

Gene Name

nuclear factor of activated T cells 5

Synonyms

OREBP, B130038B15Rik, nfatz, TonEBP

MMRRC Submission

039936-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.901) question?

Stock #

R1918 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108020102-108106149 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 108092868 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 91 (I91T)

Ref Sequence

ENSEMBL: ENSMUSP00000122871 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075922] [ENSMUST00000077440] [ENSMUST00000125721] [ENSMUST00000133026] [ENSMUST00000144100] [ENSMUST00000151114] [ENSMUST00000147588] [ENSMUST00000169453] [ENSMUST00000154474]

AlphaFold

Q9WV30

Predicted Effect

possibly damaging
Transcript: ENSMUST00000075922
AA Change: I592T

PolyPhen 2 Score 0.495 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000075311
Gene: ENSMUSG00000003847
AA Change: I592T

Domain	Start	End	E-Value	Type
low complexity region	52	98	N/A	INTRINSIC
low complexity region	179	192	N/A	INTRINSIC
Pfam:RHD	282	439	7.8e-23	PFAM
IPT	444	542	3.33e-15	SMART
low complexity region	647	653	N/A	INTRINSIC
low complexity region	734	754	N/A	INTRINSIC
low complexity region	793	810	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	915	920	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000077440
AA Change: I516T

PolyPhen 2 Score 0.442 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000076653
Gene: ENSMUSG00000003847
AA Change: I516T

Domain	Start	End	E-Value	Type
low complexity region	6	22	N/A	INTRINSIC
low complexity region	103	116	N/A	INTRINSIC
Pfam:RHD	206	363	1.5e-22	PFAM
IPT	368	466	3.33e-15	SMART
low complexity region	571	577	N/A	INTRINSIC
low complexity region	658	678	N/A	INTRINSIC
low complexity region	717	734	N/A	INTRINSIC
low complexity region	822	834	N/A	INTRINSIC
low complexity region	839	844	N/A	INTRINSIC
low complexity region	887	901	N/A	INTRINSIC
internal_repeat_2	927	1110	7.13e-8	PROSPERO
internal_repeat_1	935	1128	2.59e-11	PROSPERO
internal_repeat_2	1122	1324	7.13e-8	PROSPERO
internal_repeat_1	1207	1426	2.59e-11	PROSPERO

Predicted Effect

possibly damaging
Transcript: ENSMUST00000125721
AA Change: I592T

PolyPhen 2 Score 0.495 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000116094
Gene: ENSMUSG00000003847
AA Change: I592T

Domain	Start	End	E-Value	Type
low complexity region	52	98	N/A	INTRINSIC
low complexity region	179	192	N/A	INTRINSIC
Pfam:RHD	282	439	1e-22	PFAM
IPT	444	542	3.33e-15	SMART
low complexity region	647	653	N/A	INTRINSIC
low complexity region	734	754	N/A	INTRINSIC
low complexity region	793	810	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	915	920	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC
internal_repeat_2	1003	1186	2.22e-8	PROSPERO
internal_repeat_1	1011	1204	5.31e-12	PROSPERO
internal_repeat_2	1198	1400	2.22e-8	PROSPERO
internal_repeat_1	1283	1502	5.31e-12	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000126333

SMART Domains

Protein: ENSMUSP00000118130
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
Pfam:RHD_DNA_bind	30	132	6.8e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126397

Predicted Effect

probably benign
Transcript: ENSMUST00000133026

SMART Domains

Protein: ENSMUSP00000116631
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
low complexity region	70	88	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000144100

Predicted Effect

probably benign
Transcript: ENSMUST00000151114
AA Change: I610T

PolyPhen 2 Score 0.442 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000119370
Gene: ENSMUSG00000003847
AA Change: I610T

Domain	Start	End	E-Value	Type
low complexity region	70	116	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
Pfam:RHD_DNA_bind	300	457	1.1e-22	PFAM
IPT	462	560	3.33e-15	SMART
low complexity region	665	671	N/A	INTRINSIC
low complexity region	752	772	N/A	INTRINSIC
low complexity region	811	828	N/A	INTRINSIC
low complexity region	916	928	N/A	INTRINSIC
low complexity region	933	938	N/A	INTRINSIC
low complexity region	981	995	N/A	INTRINSIC
internal_repeat_2	1021	1204	2.24e-8	PROSPERO
internal_repeat_1	1029	1222	5.32e-12	PROSPERO
internal_repeat_2	1216	1418	2.24e-8	PROSPERO
internal_repeat_1	1301	1520	5.32e-12	PROSPERO

Predicted Effect

probably damaging
Transcript: ENSMUST00000147588
AA Change: I91T

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000122871
Gene: ENSMUSG00000003847
AA Change: I91T

Domain	Start	End	E-Value	Type
Blast:IPT	1	42	3e-20	BLAST
PDB:1IMH\|D	1	44	2e-20	PDB
SCOP:d1bfta_	1	44	3e-14	SMART
low complexity region	146	152	N/A	INTRINSIC
low complexity region	233	253	N/A	INTRINSIC
low complexity region	292	309	N/A	INTRINSIC
low complexity region	397	409	N/A	INTRINSIC
low complexity region	414	419	N/A	INTRINSIC
low complexity region	462	476	N/A	INTRINSIC
internal_repeat_2	502	685	1.17e-6	PROSPERO
internal_repeat_1	510	703	1.39e-9	PROSPERO
internal_repeat_2	697	899	1.17e-6	PROSPERO
internal_repeat_1	782	1001	1.39e-9	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000169453
AA Change: I610T

PolyPhen 2 Score 0.442 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000127784
Gene: ENSMUSG00000003847
AA Change: I610T

Domain	Start	End	E-Value	Type
low complexity region	70	116	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
Pfam:RHD_DNA_bind	300	457	1.1e-22	PFAM
IPT	462	560	3.33e-15	SMART
low complexity region	665	671	N/A	INTRINSIC
low complexity region	752	772	N/A	INTRINSIC
low complexity region	811	828	N/A	INTRINSIC
low complexity region	916	928	N/A	INTRINSIC
low complexity region	933	938	N/A	INTRINSIC
low complexity region	981	995	N/A	INTRINSIC
internal_repeat_2	1021	1204	2.24e-8	PROSPERO
internal_repeat_1	1029	1222	5.32e-12	PROSPERO
internal_repeat_2	1216	1418	2.24e-8	PROSPERO
internal_repeat_1	1301	1520	5.32e-12	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000154474

SMART Domains

Protein: ENSMUSP00000115036
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
low complexity region	52	70	N/A	INTRINSIC

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.7%
20x: 93.5%

Validation Efficiency

97% (113/116)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for one of several knock-out allele exhibit lethality between E14.5 and E17.5 as well as around P10 with kidney, cardiac or immune defects depending on the allele. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	T	G	7: 29,273,514 (GRCm39)		noncoding transcript	Het
A2m	T	C	6: 121,621,895 (GRCm39)	S314P	probably benign	Het
Abcc9	T	A	6: 142,643,408 (GRCm39)	I47F	probably damaging	Het
Abcd2	C	T	15: 91,075,684 (GRCm39)	R43H	probably benign	Het
Adgre5	A	G	8: 84,455,738 (GRCm39)	V190A	probably damaging	Het
Aen	C	T	7: 78,555,777 (GRCm39)	H242Y	possibly damaging	Het
Arfgef1	C	T	1: 10,270,103 (GRCm39)	A349T	probably benign	Het
Arhgap10	T	C	8: 77,985,708 (GRCm39)	I698V	probably benign	Het
Arpp21	C	T	9: 111,948,246 (GRCm39)		probably benign	Het
Atp10a	T	C	7: 58,477,683 (GRCm39)	I1294T	possibly damaging	Het
Atp13a2	T	C	4: 140,723,682 (GRCm39)	Y337H	possibly damaging	Het
Bsn	C	T	9: 107,984,772 (GRCm39)	G3094D	unknown	Het
Cadm2	G	A	16: 66,544,270 (GRCm39)		probably benign	Het
Cadps	T	C	14: 12,546,372 (GRCm38)	M495V	probably damaging	Het
Cass4	A	G	2: 172,269,259 (GRCm39)	H447R	possibly damaging	Het
Ccdc124	C	A	8: 71,321,588 (GRCm39)	R108L	probably benign	Het
Ccdc141	C	T	2: 76,845,047 (GRCm39)	R1340Q	probably benign	Het
Ccdc148	T	G	2: 58,872,911 (GRCm39)	R299S	probably damaging	Het
Cd300lg	A	G	11: 101,944,936 (GRCm39)	E382G	probably damaging	Het
Cd36	A	T	5: 18,002,034 (GRCm39)	C322*	probably null	Het
Celsr2	C	T	3: 108,305,966 (GRCm39)	G2046D	probably benign	Het
Clasrp	C	T	7: 19,319,188 (GRCm39)	W492*	probably null	Het
Cmtr1	T	C	17: 29,897,983 (GRCm39)	V154A	possibly damaging	Het
Ctnnb1	C	T	9: 120,780,100 (GRCm39)	P128S	possibly damaging	Het
Cyp11a1	A	G	9: 57,934,040 (GRCm39)	I496V	probably damaging	Het
Disp2	G	A	2: 118,622,408 (GRCm39)	V1047I	probably benign	Het
Dync2i1	A	C	12: 116,196,221 (GRCm39)	S509A	probably damaging	Het
Eps8l2	T	C	7: 140,941,637 (GRCm39)	V636A	probably damaging	Het
Fars2	T	C	13: 36,388,529 (GRCm39)	L6P	probably damaging	Het
Fem1al	A	G	11: 29,774,039 (GRCm39)	S473P	probably benign	Het
Fnip1	C	T	11: 54,371,510 (GRCm39)	T177I	probably damaging	Het
Fut8	G	T	12: 77,378,992 (GRCm39)	R31L	probably benign	Het
G6pd2	T	C	5: 61,967,664 (GRCm39)	F480L	probably benign	Het
Glipr1l2	T	A	10: 111,928,550 (GRCm39)	C148*	probably null	Het
Gm10142	G	A	10: 77,551,821 (GRCm39)	V61M	probably benign	Het
Gm5134	T	A	10: 75,812,180 (GRCm39)	M145K	possibly damaging	Het
Gnpda1	T	C	18: 38,466,243 (GRCm39)		probably null	Het
Gpc2	T	C	5: 138,276,641 (GRCm39)	T162A	probably benign	Het
Gtf2h4	G	A	17: 35,981,090 (GRCm39)	L246F	possibly damaging	Het
Hal	C	T	10: 93,332,469 (GRCm39)	P294S	probably damaging	Het
Hectd3	C	T	4: 116,857,540 (GRCm39)	A573V	possibly damaging	Het
Hephl1	T	A	9: 14,988,114 (GRCm39)	I665F	probably benign	Het
Herc1	T	C	9: 66,383,408 (GRCm39)		probably null	Het
Hif1an	A	G	19: 44,559,551 (GRCm39)		probably null	Het
Iho1	T	A	9: 108,290,184 (GRCm39)	H140L	probably benign	Het
Il12rb1	T	A	8: 71,266,324 (GRCm39)	M223K	probably benign	Het
Ilf3	C	T	9: 21,305,010 (GRCm39)	T201M	probably damaging	Het
Inpp5f	T	C	7: 128,265,693 (GRCm39)		probably benign	Het
Jcad	T	C	18: 4,674,292 (GRCm39)	Y685H	probably damaging	Het
Kcnj1	C	A	9: 32,308,034 (GRCm39)	Q153K	probably benign	Het
Kctd18	A	C	1: 57,998,379 (GRCm39)	H73Q	probably damaging	Het
Klhl36	T	A	8: 120,603,463 (GRCm39)	W573R	probably damaging	Het
Ldc1	A	T	4: 130,105,186 (GRCm39)	V328D	probably benign	Het
Lepr	A	G	4: 101,630,033 (GRCm39)	T583A	probably benign	Het
Ltbp4	G	A	7: 27,036,994 (GRCm39)		probably benign	Het
Mapt	A	T	11: 104,189,325 (GRCm39)	E114D	probably benign	Het
Mib1	T	A	18: 10,740,972 (GRCm39)		probably null	Het
Mthfd1	G	T	12: 76,361,750 (GRCm39)	A119S	probably damaging	Het
Mylk4	A	T	13: 32,908,836 (GRCm39)	D90E	probably benign	Het
Nceh1	T	G	3: 27,237,324 (GRCm39)	L33R	probably damaging	Het
Nxn	A	G	11: 76,152,498 (GRCm39)		probably benign	Het
Oasl1	G	A	5: 115,061,528 (GRCm39)	A20T	possibly damaging	Het
Or2m12	A	T	16: 19,105,052 (GRCm39)	M147K	probably benign	Het
Or4c127	T	C	2: 89,832,918 (GRCm39)	F56S	probably benign	Het
Or4d6	A	G	19: 12,086,871 (GRCm39)	V13A	probably benign	Het
Or5b97	A	G	19: 12,878,215 (GRCm39)	*310Q	probably null	Het
Or5t18	C	T	2: 86,637,171 (GRCm39)	M57I	probably damaging	Het
Or6c2	T	A	10: 129,362,918 (GRCm39)	V274D	probably damaging	Het
Pepd	T	C	7: 34,671,101 (GRCm39)	V215A	probably benign	Het
Pfkl	A	T	10: 77,837,260 (GRCm39)	N104K	probably damaging	Het
Phf24	T	C	4: 42,938,165 (GRCm39)		probably benign	Het
Pink1	T	C	4: 138,041,331 (GRCm39)	N530S	probably benign	Het
Pou3f2	T	C	4: 22,487,119 (GRCm39)	D338G	probably damaging	Het
Ppp1r13b	A	G	12: 111,801,244 (GRCm39)	V480A	probably damaging	Het
Ptgfrn	A	T	3: 100,963,623 (GRCm39)	I663N	probably benign	Het
Rad54b	A	C	4: 11,601,693 (GRCm39)	N416T	probably damaging	Het
Rasef	A	G	4: 73,662,351 (GRCm39)	S200P	possibly damaging	Het
Rbm33	T	A	5: 28,592,915 (GRCm39)	I605N	probably damaging	Het
Rps19bp1	T	C	15: 80,148,280 (GRCm39)	T31A	probably benign	Het
Ryr2	T	A	13: 11,571,584 (GRCm39)	T4885S	possibly damaging	Het
Serpinb6b	A	G	13: 33,162,223 (GRCm39)	I222V	probably benign	Het
Sik3	C	G	9: 46,132,387 (GRCm39)	H1276Q	probably benign	Het
Skor2	T	C	18: 76,947,051 (GRCm39)	S258P	unknown	Het
Slc22a29	C	T	19: 8,195,123 (GRCm39)		probably null	Het
Slc8a3	A	T	12: 81,361,618 (GRCm39)	F400L	probably damaging	Het
Slc9a8	T	C	2: 167,266,134 (GRCm39)	I37T	possibly damaging	Het
Smchd1	A	G	17: 71,714,232 (GRCm39)	I877T	possibly damaging	Het
Spag5	A	G	11: 78,195,002 (GRCm39)	N103S	probably benign	Het
Sptbn1	A	T	11: 30,092,414 (GRCm39)	F450L	probably damaging	Het
Strn4	T	A	7: 16,567,846 (GRCm39)	Y507N	probably damaging	Het
Stxbp5	C	A	10: 9,688,042 (GRCm39)	V420F	possibly damaging	Het
Syt17	G	T	7: 118,033,208 (GRCm39)	L267I	possibly damaging	Het
Tcstv4	T	C	13: 120,769,758 (GRCm39)	L26P	probably damaging	Het
Tdo2	T	A	3: 81,866,247 (GRCm39)	R339W	probably damaging	Het
Tex55	A	G	16: 38,648,450 (GRCm39)	Y220H	possibly damaging	Het
Timd5	A	G	11: 46,419,358 (GRCm39)	D58G	possibly damaging	Het
Ttn	A	G	2: 76,571,745 (GRCm39)	S26383P	probably damaging	Het
Ttn	G	T	2: 76,638,868 (GRCm39)	T13938K	probably damaging	Het
Tulp2	A	G	7: 45,167,365 (GRCm39)	N188D	possibly damaging	Het
Ube3c	C	T	5: 29,792,315 (GRCm39)	R37C	probably damaging	Het
Uggt2	G	T	14: 119,245,467 (GRCm39)		probably benign	Het
Umodl1	G	A	17: 31,203,017 (GRCm39)	V457M	probably damaging	Het
Usp17la	C	T	7: 104,509,953 (GRCm39)	T186I	probably benign	Het
Vmn1r226	T	C	17: 20,907,842 (GRCm39)	S25P	probably damaging	Het
Vmn1r235	T	A	17: 21,482,659 (GRCm39)	I328K	possibly damaging	Het
Vmn2r50	A	T	7: 9,781,610 (GRCm39)	S378R	probably benign	Het
Vwa2	C	T	19: 56,897,366 (GRCm39)	T557I	probably benign	Het
Vxn	T	C	1: 9,671,852 (GRCm39)	F15S	probably damaging	Het
Yes1	G	T	5: 32,842,079 (GRCm39)	Q534H	probably benign	Het
Zbtb25	A	T	12: 76,396,075 (GRCm39)	Y382*	probably null	Het
Zc3h3	G	A	15: 75,648,967 (GRCm39)	P722S	probably damaging	Het
Zfp36l2	T	C	17: 84,494,164 (GRCm39)	T158A	probably damaging	Het
Zfp536	T	C	7: 37,179,624 (GRCm39)	T994A	probably damaging	Het
Zfp592	C	T	7: 80,687,168 (GRCm39)	Q824*	probably null	Het
Zfp629	C	A	7: 127,211,172 (GRCm39)	K212N	probably damaging	Het
Zfp930	C	A	8: 69,681,357 (GRCm39)	Q350K	probably benign	Het

Other mutations in Nfat5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Nfat5	APN	8	108,094,146 (GRCm39)	missense	probably damaging	1.00
IGL01145:Nfat5	APN	8	108,093,847 (GRCm39)	missense	probably damaging	0.99
IGL01700:Nfat5	APN	8	108,065,762 (GRCm39)	missense	probably damaging	0.99
IGL01721:Nfat5	APN	8	108,071,611 (GRCm39)	critical splice donor site	probably null
IGL01796:Nfat5	APN	8	108,094,273 (GRCm39)	missense	probably damaging	1.00
IGL01976:Nfat5	APN	8	108,094,191 (GRCm39)	missense	probably damaging	1.00
IGL02063:Nfat5	APN	8	108,088,450 (GRCm39)	missense	probably benign	0.03
IGL02150:Nfat5	APN	8	108,094,584 (GRCm39)	nonsense	probably null
IGL02174:Nfat5	APN	8	108,065,683 (GRCm39)	missense	probably damaging	1.00
IGL02224:Nfat5	APN	8	108,071,447 (GRCm39)	missense	probably benign	0.00
IGL02226:Nfat5	APN	8	108,078,154 (GRCm39)	nonsense	probably null
IGL02324:Nfat5	APN	8	108,092,808 (GRCm39)	splice site	probably benign
IGL02724:Nfat5	APN	8	108,085,367 (GRCm39)	missense	probably damaging	0.97
fettfeld	UTSW	8	108,074,359 (GRCm39)	missense	probably damaging	1.00
Grunefeld	UTSW	8	108,082,140 (GRCm39)	splice site	probably null
Kleinfeld	UTSW	8	108,078,070 (GRCm39)	missense	probably damaging	1.00
Lisa	UTSW	8	108,074,321 (GRCm39)	missense	probably damaging	1.00
viola	UTSW	8	108,085,300 (GRCm39)	missense	probably damaging	1.00
H8562:Nfat5	UTSW	8	108,066,014 (GRCm39)	splice site	probably benign
R0003:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0117:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0118:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0119:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0135:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0138:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0141:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0302:Nfat5	UTSW	8	108,085,333 (GRCm39)	missense	probably damaging	1.00
R0420:Nfat5	UTSW	8	108,094,093 (GRCm39)	missense	probably damaging	1.00
R0613:Nfat5	UTSW	8	108,092,927 (GRCm39)	missense	possibly damaging	0.83
R0691:Nfat5	UTSW	8	108,082,237 (GRCm39)	missense	probably damaging	1.00
R0743:Nfat5	UTSW	8	108,094,698 (GRCm39)	missense	probably damaging	1.00
R1329:Nfat5	UTSW	8	108,095,659 (GRCm39)	missense	probably benign	0.42
R1550:Nfat5	UTSW	8	108,097,205 (GRCm39)	missense	probably damaging	0.99
R1590:Nfat5	UTSW	8	108,020,522 (GRCm39)	missense	probably damaging	1.00
R1778:Nfat5	UTSW	8	108,088,421 (GRCm39)	missense	probably damaging	1.00
R1827:Nfat5	UTSW	8	108,093,966 (GRCm39)	missense	probably benign	0.00
R2679:Nfat5	UTSW	8	108,071,546 (GRCm39)	missense	probably damaging	1.00
R2850:Nfat5	UTSW	8	108,020,492 (GRCm39)	missense	probably damaging	1.00
R3703:Nfat5	UTSW	8	108,078,053 (GRCm39)	splice site	probably benign
R3966:Nfat5	UTSW	8	108,093,921 (GRCm39)	missense	possibly damaging	0.47
R4301:Nfat5	UTSW	8	108,082,327 (GRCm39)	intron	probably benign
R4596:Nfat5	UTSW	8	108,078,132 (GRCm39)	missense	possibly damaging	0.93
R4602:Nfat5	UTSW	8	108,093,855 (GRCm39)	nonsense	probably null
R4627:Nfat5	UTSW	8	108,095,908 (GRCm39)	missense	probably damaging	1.00
R4917:Nfat5	UTSW	8	108,051,284 (GRCm39)	missense	probably damaging	1.00
R4918:Nfat5	UTSW	8	108,051,284 (GRCm39)	missense	probably damaging	1.00
R5089:Nfat5	UTSW	8	108,078,070 (GRCm39)	missense	probably damaging	1.00
R5495:Nfat5	UTSW	8	108,095,079 (GRCm39)	missense	probably benign	0.03
R5566:Nfat5	UTSW	8	108,095,767 (GRCm39)	missense	possibly damaging	0.47
R5851:Nfat5	UTSW	8	108,074,359 (GRCm39)	missense	probably damaging	1.00
R6012:Nfat5	UTSW	8	108,093,765 (GRCm39)	missense	probably benign	0.09
R6018:Nfat5	UTSW	8	108,082,283 (GRCm39)	critical splice donor site	probably null
R6364:Nfat5	UTSW	8	108,094,909 (GRCm39)	missense	probably benign	0.00
R6404:Nfat5	UTSW	8	108,097,220 (GRCm39)	missense	probably benign	0.01
R6466:Nfat5	UTSW	8	108,082,140 (GRCm39)	splice site	probably null
R7056:Nfat5	UTSW	8	108,094,738 (GRCm39)	missense	probably damaging	1.00
R7105:Nfat5	UTSW	8	108,095,823 (GRCm39)	missense	possibly damaging	0.88
R7128:Nfat5	UTSW	8	108,085,323 (GRCm39)	missense	probably benign	0.10
R7214:Nfat5	UTSW	8	108,020,515 (GRCm39)	missense	probably damaging	0.99
R7276:Nfat5	UTSW	8	108,093,731 (GRCm39)	missense	probably benign	0.25
R7560:Nfat5	UTSW	8	108,097,221 (GRCm39)	missense	probably benign	0.15
R7844:Nfat5	UTSW	8	108,085,300 (GRCm39)	missense	probably damaging	1.00
R7993:Nfat5	UTSW	8	108,082,134 (GRCm39)	splice site	probably null
R8407:Nfat5	UTSW	8	108,094,047 (GRCm39)	nonsense	probably null
R8428:Nfat5	UTSW	8	108,095,152 (GRCm39)	missense	probably damaging	0.96
R8798:Nfat5	UTSW	8	108,074,321 (GRCm39)	missense	probably damaging	1.00
R8919:Nfat5	UTSW	8	108,095,228 (GRCm39)	missense	probably damaging	0.99
R9067:Nfat5	UTSW	8	108,094,536 (GRCm39)	missense	probably benign	0.07
R9123:Nfat5	UTSW	8	108,078,141 (GRCm39)	missense	probably damaging	0.97
R9226:Nfat5	UTSW	8	108,095,401 (GRCm39)	missense	probably damaging	1.00
R9351:Nfat5	UTSW	8	108,065,910 (GRCm39)	missense	probably damaging	1.00
X0022:Nfat5	UTSW	8	108,074,388 (GRCm39)	nonsense	probably null
Z1177:Nfat5	UTSW	8	108,065,474 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCACACTTGCGTAAAGGGAG -3'
(R):5'- CATCTCATGGAAAACAGATGCC -3'

Sequencing Primer
(F):5'- TGCCTAGGATGCATTAAGCC -3'
(R):5'- TGGAAAACAGATGCCAACAAAACAAG -3'

Posted On

2014-07-14