Mutagenetix > Incidental Mutation

Incidental Mutation 'R1923:Mroh9'

213198

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

039941-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1923 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

162851871-162913239 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 162903860 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Cysteine at position 51 (S51C)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

probably damaging
Transcript: ENSMUST00000096608
AA Change: S51C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: S51C

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Meta Mutation Damage Score

0.4405

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.5%
20x: 92.9%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610008E11Rik	T	C	10: 78,903,743 (GRCm39)	E163G	probably damaging	Het
4921513D11Rik	A	G	17: 79,935,562 (GRCm39)		probably benign	Het
Acox3	T	A	5: 35,749,459 (GRCm39)	F195I	possibly damaging	Het
Acsl6	T	C	11: 54,216,417 (GRCm39)	Y135H	probably damaging	Het
Ankhd1	C	A	18: 36,781,083 (GRCm39)	A2045E	probably benign	Het
Ap5z1	A	G	5: 142,458,096 (GRCm39)	H423R	probably benign	Het
Arsk	T	A	13: 76,214,985 (GRCm39)		probably benign	Het
Bpi	G	A	2: 158,103,083 (GRCm39)	G76D	probably damaging	Het
Carmil3	A	G	14: 55,739,861 (GRCm39)	T983A	probably damaging	Het
Ccdc192	A	T	18: 57,666,959 (GRCm39)	D12V	probably damaging	Het
Chmp2b	A	T	16: 65,342,213 (GRCm39)	M125K	possibly damaging	Het
Clca3a2	T	A	3: 144,511,491 (GRCm39)	I26L	probably damaging	Het
Cubn	T	C	2: 13,315,337 (GRCm39)	Y3032C	probably damaging	Het
Ehbp1	T	A	11: 22,101,850 (GRCm39)	D226V	probably damaging	Het
Enpp6	A	T	8: 47,535,541 (GRCm39)	D362V	probably damaging	Het
Fam53b	A	G	7: 132,317,521 (GRCm39)	S374P	probably damaging	Het
Fmn1	T	C	2: 113,260,066 (GRCm39)		probably benign	Het
Fndc7	C	T	3: 108,784,003 (GRCm39)	R202H	probably benign	Het
Frmd4b	T	G	6: 97,265,415 (GRCm39)	D951A	probably benign	Het
Git2	A	G	5: 114,877,162 (GRCm39)	Y107H	probably damaging	Het
Gm11595	A	T	11: 99,663,365 (GRCm39)	V105E	unknown	Het
Krt26	CTAGTA	CTA	11: 99,224,352 (GRCm39)		probably benign	Het
Lrriq4	C	A	3: 30,713,242 (GRCm39)	Q448K	probably benign	Het
Ly6g5c	A	G	17: 35,330,863 (GRCm39)	I128M	possibly damaging	Het
Myh3	A	G	11: 66,970,828 (GRCm39)	S2G	probably benign	Het
Nat8f4	A	G	6: 85,878,497 (GRCm39)	Y9H	probably damaging	Het
Negr1	T	C	3: 156,267,836 (GRCm39)	V2A	probably benign	Het
Or2y1	C	T	11: 49,386,131 (GRCm39)	T257I	probably damaging	Het
Or7g19	C	T	9: 18,856,781 (GRCm39)	T279I	probably benign	Het
Or8k32	T	G	2: 86,368,857 (GRCm39)	Y132S	probably damaging	Het
Otog	G	A	7: 45,895,707 (GRCm39)	C107Y	probably damaging	Het
Pdgfra	C	A	5: 75,324,394 (GRCm39)	T83K	probably benign	Het
Pdlim2	C	T	14: 70,402,228 (GRCm39)	R296H	probably damaging	Het
Retreg1	A	G	15: 25,969,924 (GRCm39)	D109G	probably damaging	Het
Rgs12	T	A	5: 35,189,613 (GRCm39)	W674R	probably damaging	Het
Rgs18	T	C	1: 144,631,818 (GRCm39)	D98G	possibly damaging	Het
Rnf146	A	G	10: 29,223,715 (GRCm39)	F57S	probably damaging	Het
Skic3	T	A	13: 76,282,889 (GRCm39)	V737D	probably damaging	Het
Slc22a20	C	A	19: 6,021,464 (GRCm39)	V513L	probably benign	Het
Stox2	G	T	8: 47,646,661 (GRCm39)	F266L	probably damaging	Het
Tbx21	C	T	11: 96,990,863 (GRCm39)	V272M	probably damaging	Het
Tmc7	A	G	7: 118,144,850 (GRCm39)	F570S	probably benign	Het
Tmem236	T	C	2: 14,224,117 (GRCm39)	I302T	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trpm3	T	C	19: 22,862,776 (GRCm39)	L506S	probably damaging	Het
Uggt1	T	C	1: 36,218,694 (GRCm39)	I78V	probably damaging	Het
Xpo4	A	T	14: 57,828,328 (GRCm39)	V844D	probably damaging	Het
Zfp507	C	T	7: 35,493,150 (GRCm39)	R631Q	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	162,873,350 (GRCm39)	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	162,907,072 (GRCm39)	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	162,852,227 (GRCm39)	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	162,888,191 (GRCm39)	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	162,866,700 (GRCm39)	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	162,875,435 (GRCm39)	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	162,908,144 (GRCm39)	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	162,883,172 (GRCm39)	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	162,875,551 (GRCm39)	splice site	probably null
IGL01823:Mroh9	APN	1	162,883,178 (GRCm39)	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	162,890,071 (GRCm39)	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	162,885,648 (GRCm39)	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	162,903,149 (GRCm39)	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	162,908,145 (GRCm39)	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	162,852,205 (GRCm39)	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	162,854,071 (GRCm39)	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	162,866,766 (GRCm39)	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	162,888,331 (GRCm39)	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	162,893,693 (GRCm39)	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	162,871,552 (GRCm39)	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	162,854,078 (GRCm39)	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	162,852,110 (GRCm39)	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	162,852,161 (GRCm39)	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	162,884,347 (GRCm39)	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	162,873,279 (GRCm39)	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	162,866,714 (GRCm39)	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	162,854,082 (GRCm39)	missense	probably damaging	0.97
R2325:Mroh9	UTSW	1	162,854,099 (GRCm39)	splice site	probably null
R2511:Mroh9	UTSW	1	162,866,514 (GRCm39)	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	162,884,341 (GRCm39)	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	162,884,338 (GRCm39)	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	162,893,638 (GRCm39)	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	162,908,122 (GRCm39)	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	162,871,662 (GRCm39)	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	162,893,593 (GRCm39)	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	162,883,162 (GRCm39)	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	162,852,061 (GRCm39)	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	162,854,093 (GRCm39)	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	162,871,690 (GRCm39)	missense	probably damaging	0.96
R5324:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	162,908,156 (GRCm39)	start gained	probably benign
R6005:Mroh9	UTSW	1	162,903,246 (GRCm39)	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	162,893,612 (GRCm39)	nonsense	probably null
R6414:Mroh9	UTSW	1	162,902,271 (GRCm39)	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	162,903,873 (GRCm39)	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	162,866,541 (GRCm39)	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	162,885,607 (GRCm39)	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	162,903,130 (GRCm39)	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	162,873,610 (GRCm39)	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	162,903,935 (GRCm39)	missense	probably benign
R7026:Mroh9	UTSW	1	162,888,251 (GRCm39)	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	162,866,525 (GRCm39)	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	162,866,750 (GRCm39)	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	162,903,858 (GRCm39)	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	162,902,277 (GRCm39)	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	162,873,601 (GRCm39)	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	162,852,122 (GRCm39)	missense	probably benign
R7808:Mroh9	UTSW	1	162,866,678 (GRCm39)	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	162,866,802 (GRCm39)	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	162,866,544 (GRCm39)	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	162,890,096 (GRCm39)	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	162,852,294 (GRCm39)	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	162,883,250 (GRCm39)	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	162,899,595 (GRCm39)	splice site	probably null
R8960:Mroh9	UTSW	1	162,883,196 (GRCm39)	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	162,890,069 (GRCm39)	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	162,875,412 (GRCm39)	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	162,890,030 (GRCm39)	missense
R9596:Mroh9	UTSW	1	162,893,576 (GRCm39)	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	162,866,498 (GRCm39)	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	162,885,630 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGGGCAGTGTTCCTACCAC -3'
(R):5'- AAAGCACAATGGCTGGTTGG -3'

Sequencing Primer
(F):5'- GCATCTTCAACTGGATTACCAAAG -3'
(R):5'- GTCCTTAGGAGTTTAAAGCC -3'

Posted On

2014-07-14