Mutagenetix > Incidental Mutation

Incidental Mutation 'R1924:Adgrg3'

213283

Institutional Source

Beutler Lab

Gene Symbol

Adgrg3

Ensembl Gene

ENSMUSG00000060470

Gene Name

adhesion G protein-coupled receptor G3

Synonyms

Pb99, A030001G24Rik, Gpr97

MMRRC Submission

039942-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1924 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

95744320-95771878 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 95762562 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 204 (R204H)

Ref Sequence

ENSEMBL: ENSMUSP00000051079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051259]

AlphaFold

Q8R0T6

Predicted Effect

probably benign
Transcript: ENSMUST00000051259
AA Change: R204H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000051079
Gene: ENSMUSG00000060470
AA Change: R204H

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
GPS	209	256	3.45e-11	SMART
Pfam:7tm_2	260	509	5.1e-33	PFAM
low complexity region	520	531	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211974

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211994

Predicted Effect

unknown
Transcript: ENSMUST00000212570
AA Change: R167H

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

94% (63/67)

MGI Phenotype

PHENOTYPE: Homozygous null mice display normal B and T cell development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	G	A	5: 114,368,781 (GRCm39)	M1666I	possibly damaging	Het
Adamts13	A	T	2: 26,874,153 (GRCm39)	Q434L	probably damaging	Het
Arhgef28	A	C	13: 98,073,324 (GRCm39)		probably benign	Het
AU041133	T	A	10: 81,987,101 (GRCm39)	C251*	probably null	Het
Axin2	T	A	11: 108,833,794 (GRCm39)	N580K	probably benign	Het
C4b	A	G	17: 34,948,631 (GRCm39)	C1560R	probably damaging	Het
Cacna1g	A	G	11: 94,334,880 (GRCm39)	I809T	possibly damaging	Het
Cacna1s	C	A	1: 136,016,755 (GRCm39)		probably null	Het
Cadps2	T	C	6: 23,688,857 (GRCm39)	S151G	probably damaging	Het
Capn1	T	C	19: 6,040,086 (GRCm39)		probably null	Het
Capn9	A	G	8: 125,302,965 (GRCm39)	S28G	probably benign	Het
Ccdc73	A	G	2: 104,822,637 (GRCm39)	D862G	probably damaging	Het
Cdk17	T	C	10: 93,061,979 (GRCm39)	L237P	probably damaging	Het
Cfap251	A	G	5: 123,440,802 (GRCm39)	I1120V	possibly damaging	Het
Chad	A	C	11: 94,456,384 (GRCm39)	N154T	possibly damaging	Het
Cog1	C	T	11: 113,547,038 (GRCm39)	T544I	probably benign	Het
Cracd	A	T	5: 77,006,470 (GRCm39)	T944S	unknown	Het
Ctnna2	T	C	6: 76,931,830 (GRCm39)	E590G	possibly damaging	Het
Dapk2	T	A	9: 66,072,642 (GRCm39)	M6K	probably benign	Het
Dchs1	T	C	7: 105,421,487 (GRCm39)	E311G	possibly damaging	Het
Ddr2	T	A	1: 169,809,641 (GRCm39)	T779S	probably benign	Het
Dhtkd1	A	G	2: 5,916,744 (GRCm39)	V644A	probably damaging	Het
Dock2	A	G	11: 34,414,934 (GRCm39)	V147A	possibly damaging	Het
Ear10	A	T	14: 44,160,357 (GRCm39)	*157K	probably null	Het
Gm10801	T	C	2: 98,494,197 (GRCm39)	I113T	probably damaging	Het
Gmps	T	G	3: 63,906,049 (GRCm39)	C449G	probably damaging	Het
Grin3a	A	G	4: 49,844,988 (GRCm39)	S32P	possibly damaging	Het
Igkv1-115	A	T	6: 68,138,592 (GRCm39)	D65V	probably damaging	Het
Klk1b4	A	G	7: 43,859,105 (GRCm39)	N41S	probably benign	Het
Krt26	CTAGTA	CTA	11: 99,224,352 (GRCm39)		probably benign	Het
Lrrc8a	T	A	2: 30,145,262 (GRCm39)	D25E	probably damaging	Het
Muc5b	A	G	7: 141,421,960 (GRCm39)	R4426G	possibly damaging	Het
Muc6	G	A	7: 141,218,313 (GRCm39)	S2120F	possibly damaging	Het
Myo5a	G	T	9: 75,023,489 (GRCm39)	D17Y	probably damaging	Het
Nat1	C	G	8: 67,944,076 (GRCm39)	L154V	probably benign	Het
Nek3	T	A	8: 22,647,047 (GRCm39)	T163S	probably damaging	Het
Or2w25	G	T	11: 59,503,949 (GRCm39)	R53L	possibly damaging	Het
Or5p80	A	G	7: 108,229,562 (GRCm39)	D121G	probably damaging	Het
Or6c204	A	G	10: 129,023,044 (GRCm39)	I82T	possibly damaging	Het
Or8d2b	T	A	9: 38,789,147 (GRCm39)	I225N	probably damaging	Het
Or8g32	A	T	9: 39,305,163 (GRCm39)	E22D	possibly damaging	Het
Or9s13	T	C	1: 92,548,525 (GRCm39)	L299P	probably damaging	Het
Osr1	T	G	12: 9,629,268 (GRCm39)	L47R	probably damaging	Het
Pdlim2	C	T	14: 70,402,228 (GRCm39)	R296H	probably damaging	Het
Pik3ap1	T	A	19: 41,291,053 (GRCm39)	N493I	possibly damaging	Het
Polr3e	T	A	7: 120,539,820 (GRCm39)	N522K	probably damaging	Het
Rabgap1	T	A	2: 37,385,771 (GRCm39)		probably null	Het
Rp1l1	C	A	14: 64,268,992 (GRCm39)	A1526E	probably benign	Het
Scn3a	T	A	2: 65,291,878 (GRCm39)	I1623F	probably damaging	Het
Serpinb9e	A	G	13: 33,437,428 (GRCm39)	T104A	probably benign	Het
Sh3rf3	T	C	10: 58,939,989 (GRCm39)		probably benign	Het
Slc30a3	A	G	5: 31,245,748 (GRCm39)	Y213H	probably damaging	Het
Sptbn4	C	T	7: 27,106,563 (GRCm39)	R955H	probably damaging	Het
Tfb1m	A	T	17: 3,569,946 (GRCm39)	Y307N	probably damaging	Het
Tnrc6b	G	T	15: 80,768,407 (GRCm39)		probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Ulk1	A	G	5: 110,938,936 (GRCm39)	Y501H	probably damaging	Het
Zbtb17	A	G	4: 141,191,914 (GRCm39)	H315R	probably damaging	Het
Zeb2	A	G	2: 44,892,624 (GRCm39)	Y142H	probably damaging	Het
Zfr	T	C	15: 12,160,715 (GRCm39)	S763P	possibly damaging	Het

Other mutations in Adgrg3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01096:Adgrg3	APN	8	95,766,221 (GRCm39)	missense	possibly damaging	0.90
IGL01724:Adgrg3	APN	8	95,766,053 (GRCm39)	missense	probably benign	0.04
IGL02111:Adgrg3	APN	8	95,761,627 (GRCm39)	missense	probably damaging	0.96
IGL02142:Adgrg3	APN	8	95,766,483 (GRCm39)	missense	probably damaging	1.00
IGL02577:Adgrg3	APN	8	95,766,555 (GRCm39)	missense	probably damaging	0.99
IGL02940:Adgrg3	APN	8	95,760,084 (GRCm39)	missense	possibly damaging	0.48
IGL03395:Adgrg3	APN	8	95,761,701 (GRCm39)	missense	probably damaging	1.00
R0111:Adgrg3	UTSW	8	95,761,738 (GRCm39)	splice site	probably benign
R0288:Adgrg3	UTSW	8	95,766,568 (GRCm39)	missense	possibly damaging	0.92
R0403:Adgrg3	UTSW	8	95,763,550 (GRCm39)	missense	probably benign	0.07
R1553:Adgrg3	UTSW	8	95,766,896 (GRCm39)	missense	possibly damaging	0.90
R1667:Adgrg3	UTSW	8	95,760,001 (GRCm39)	nonsense	probably null
R1686:Adgrg3	UTSW	8	95,759,997 (GRCm39)	missense	probably benign	0.02
R1872:Adgrg3	UTSW	8	95,760,070 (GRCm39)	missense	possibly damaging	0.87
R1882:Adgrg3	UTSW	8	95,766,943 (GRCm39)	missense	probably benign	0.03
R1998:Adgrg3	UTSW	8	95,763,296 (GRCm39)	missense	probably damaging	1.00
R2090:Adgrg3	UTSW	8	95,766,558 (GRCm39)	missense	possibly damaging	0.54
R2696:Adgrg3	UTSW	8	95,747,702 (GRCm39)	missense	probably benign	0.01
R3846:Adgrg3	UTSW	8	95,767,049 (GRCm39)	missense	probably benign	0.07
R4013:Adgrg3	UTSW	8	95,761,727 (GRCm39)	splice site	probably benign
R4405:Adgrg3	UTSW	8	95,763,536 (GRCm39)	missense	probably benign	0.15
R4622:Adgrg3	UTSW	8	95,767,153 (GRCm39)	missense	probably damaging	1.00
R4878:Adgrg3	UTSW	8	95,761,714 (GRCm39)	missense	possibly damaging	0.86
R5101:Adgrg3	UTSW	8	95,763,563 (GRCm39)	missense	probably benign	0.00
R5309:Adgrg3	UTSW	8	95,766,492 (GRCm39)	missense	probably benign	0.00
R5312:Adgrg3	UTSW	8	95,766,492 (GRCm39)	missense	probably benign	0.00
R5353:Adgrg3	UTSW	8	95,762,556 (GRCm39)	missense	probably damaging	0.99
R5820:Adgrg3	UTSW	8	95,766,221 (GRCm39)	missense	possibly damaging	0.90
R6240:Adgrg3	UTSW	8	95,766,544 (GRCm39)	missense	probably benign	0.23
R6272:Adgrg3	UTSW	8	95,762,889 (GRCm39)	missense	noncoding transcript
R7110:Adgrg3	UTSW	8	95,761,591 (GRCm39)	missense	possibly damaging	0.62
R7645:Adgrg3	UTSW	8	95,761,392 (GRCm39)	intron	probably benign
R8178:Adgrg3	UTSW	8	95,761,675 (GRCm39)	missense	probably damaging	0.98
R8397:Adgrg3	UTSW	8	95,767,141 (GRCm39)	missense	probably benign	0.01
R8730:Adgrg3	UTSW	8	95,766,556 (GRCm39)	missense	probably benign	0.09
R8951:Adgrg3	UTSW	8	95,761,362 (GRCm39)	intron	probably benign
R9100:Adgrg3	UTSW	8	95,762,891 (GRCm39)	intron	probably benign
R9523:Adgrg3	UTSW	8	95,766,186 (GRCm39)	missense	probably benign	0.06
R9583:Adgrg3	UTSW	8	95,760,071 (GRCm39)	missense	probably benign	0.00
R9589:Adgrg3	UTSW	8	95,760,093 (GRCm39)	missense	possibly damaging	0.87
X0017:Adgrg3	UTSW	8	95,744,398 (GRCm39)	start codon destroyed	probably null	0.53

Predicted Primers

PCR Primer
(F):5'- AAGGTCAGCCCATCCCATAG -3'
(R):5'- TCCCCACACATTTATGAACATGGG -3'

Sequencing Primer
(F):5'- ATAGCTAGCTTCCTGCCAATC -3'
(R):5'- CACACATTTATGAACATGGGGTGGG -3'

Posted On

2014-07-14