Mutagenetix > Incidental Mutation

Incidental Mutation 'R1924:Or8g32'

213286

Institutional Source

Beutler Lab

Gene Symbol

Or8g32

Ensembl Gene

ENSMUSG00000094269

Gene Name

olfactory receptor family 8 subfamily G member 32

Synonyms

GA_x6K02T2PVTD-33090395-33091330, MOR171-33P, MOR171-49, Olfr951

MMRRC Submission

039942-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R1924 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

39305089-39306033 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 39305163 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Aspartic acid at position 22 (E22D)

Ref Sequence

ENSEMBL: ENSMUSP00000150976 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078531] [ENSMUST00000216107]

AlphaFold

Q9EQ94

Predicted Effect

possibly damaging
Transcript: ENSMUST00000078531
AA Change: E25D

PolyPhen 2 Score 0.890 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000077615
Gene: ENSMUSG00000094269
AA Change: E25D

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	2.5e-52	PFAM
Pfam:7tm_1	44	293	5e-19	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000216107
AA Change: E22D

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

94% (63/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	G	A	5: 114,368,781 (GRCm39)	M1666I	possibly damaging	Het
Adamts13	A	T	2: 26,874,153 (GRCm39)	Q434L	probably damaging	Het
Adgrg3	G	A	8: 95,762,562 (GRCm39)	R204H	probably benign	Het
Arhgef28	A	C	13: 98,073,324 (GRCm39)		probably benign	Het
AU041133	T	A	10: 81,987,101 (GRCm39)	C251*	probably null	Het
Axin2	T	A	11: 108,833,794 (GRCm39)	N580K	probably benign	Het
C4b	A	G	17: 34,948,631 (GRCm39)	C1560R	probably damaging	Het
Cacna1g	A	G	11: 94,334,880 (GRCm39)	I809T	possibly damaging	Het
Cacna1s	C	A	1: 136,016,755 (GRCm39)		probably null	Het
Cadps2	T	C	6: 23,688,857 (GRCm39)	S151G	probably damaging	Het
Capn1	T	C	19: 6,040,086 (GRCm39)		probably null	Het
Capn9	A	G	8: 125,302,965 (GRCm39)	S28G	probably benign	Het
Ccdc73	A	G	2: 104,822,637 (GRCm39)	D862G	probably damaging	Het
Cdk17	T	C	10: 93,061,979 (GRCm39)	L237P	probably damaging	Het
Cfap251	A	G	5: 123,440,802 (GRCm39)	I1120V	possibly damaging	Het
Chad	A	C	11: 94,456,384 (GRCm39)	N154T	possibly damaging	Het
Cog1	C	T	11: 113,547,038 (GRCm39)	T544I	probably benign	Het
Cracd	A	T	5: 77,006,470 (GRCm39)	T944S	unknown	Het
Ctnna2	T	C	6: 76,931,830 (GRCm39)	E590G	possibly damaging	Het
Dapk2	T	A	9: 66,072,642 (GRCm39)	M6K	probably benign	Het
Dchs1	T	C	7: 105,421,487 (GRCm39)	E311G	possibly damaging	Het
Ddr2	T	A	1: 169,809,641 (GRCm39)	T779S	probably benign	Het
Dhtkd1	A	G	2: 5,916,744 (GRCm39)	V644A	probably damaging	Het
Dock2	A	G	11: 34,414,934 (GRCm39)	V147A	possibly damaging	Het
Ear10	A	T	14: 44,160,357 (GRCm39)	*157K	probably null	Het
Gm10801	T	C	2: 98,494,197 (GRCm39)	I113T	probably damaging	Het
Gmps	T	G	3: 63,906,049 (GRCm39)	C449G	probably damaging	Het
Grin3a	A	G	4: 49,844,988 (GRCm39)	S32P	possibly damaging	Het
Igkv1-115	A	T	6: 68,138,592 (GRCm39)	D65V	probably damaging	Het
Klk1b4	A	G	7: 43,859,105 (GRCm39)	N41S	probably benign	Het
Krt26	CTAGTA	CTA	11: 99,224,352 (GRCm39)		probably benign	Het
Lrrc8a	T	A	2: 30,145,262 (GRCm39)	D25E	probably damaging	Het
Muc5b	A	G	7: 141,421,960 (GRCm39)	R4426G	possibly damaging	Het
Muc6	G	A	7: 141,218,313 (GRCm39)	S2120F	possibly damaging	Het
Myo5a	G	T	9: 75,023,489 (GRCm39)	D17Y	probably damaging	Het
Nat1	C	G	8: 67,944,076 (GRCm39)	L154V	probably benign	Het
Nek3	T	A	8: 22,647,047 (GRCm39)	T163S	probably damaging	Het
Or2w25	G	T	11: 59,503,949 (GRCm39)	R53L	possibly damaging	Het
Or5p80	A	G	7: 108,229,562 (GRCm39)	D121G	probably damaging	Het
Or6c204	A	G	10: 129,023,044 (GRCm39)	I82T	possibly damaging	Het
Or8d2b	T	A	9: 38,789,147 (GRCm39)	I225N	probably damaging	Het
Or9s13	T	C	1: 92,548,525 (GRCm39)	L299P	probably damaging	Het
Osr1	T	G	12: 9,629,268 (GRCm39)	L47R	probably damaging	Het
Pdlim2	C	T	14: 70,402,228 (GRCm39)	R296H	probably damaging	Het
Pik3ap1	T	A	19: 41,291,053 (GRCm39)	N493I	possibly damaging	Het
Polr3e	T	A	7: 120,539,820 (GRCm39)	N522K	probably damaging	Het
Rabgap1	T	A	2: 37,385,771 (GRCm39)		probably null	Het
Rp1l1	C	A	14: 64,268,992 (GRCm39)	A1526E	probably benign	Het
Scn3a	T	A	2: 65,291,878 (GRCm39)	I1623F	probably damaging	Het
Serpinb9e	A	G	13: 33,437,428 (GRCm39)	T104A	probably benign	Het
Sh3rf3	T	C	10: 58,939,989 (GRCm39)		probably benign	Het
Slc30a3	A	G	5: 31,245,748 (GRCm39)	Y213H	probably damaging	Het
Sptbn4	C	T	7: 27,106,563 (GRCm39)	R955H	probably damaging	Het
Tfb1m	A	T	17: 3,569,946 (GRCm39)	Y307N	probably damaging	Het
Tnrc6b	G	T	15: 80,768,407 (GRCm39)		probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Ulk1	A	G	5: 110,938,936 (GRCm39)	Y501H	probably damaging	Het
Zbtb17	A	G	4: 141,191,914 (GRCm39)	H315R	probably damaging	Het
Zeb2	A	G	2: 44,892,624 (GRCm39)	Y142H	probably damaging	Het
Zfr	T	C	15: 12,160,715 (GRCm39)	S763P	possibly damaging	Het

Other mutations in Or8g32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01527:Or8g32	APN	9	39,305,114 (GRCm39)	missense	probably benign	0.01
IGL01650:Or8g32	APN	9	39,305,252 (GRCm39)	missense	probably damaging	0.99
IGL02134:Or8g32	APN	9	39,305,830 (GRCm39)	missense	probably damaging	0.99
IGL03113:Or8g32	APN	9	39,305,981 (GRCm39)	missense	probably damaging	1.00
R0127:Or8g32	UTSW	9	39,305,238 (GRCm39)	missense	probably benign	0.16
R1730:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R1783:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R3785:Or8g32	UTSW	9	39,305,678 (GRCm39)	missense	probably benign	0.07
R3787:Or8g32	UTSW	9	39,305,678 (GRCm39)	missense	probably benign	0.07
R4607:Or8g32	UTSW	9	39,306,031 (GRCm39)	makesense	probably null
R4803:Or8g32	UTSW	9	39,305,932 (GRCm39)	missense	probably benign	0.26
R5314:Or8g32	UTSW	9	39,305,785 (GRCm39)	missense	probably damaging	1.00
R5338:Or8g32	UTSW	9	39,305,371 (GRCm39)	missense	probably damaging	1.00
R5360:Or8g32	UTSW	9	39,305,698 (GRCm39)	missense	probably benign	0.00
R5468:Or8g32	UTSW	9	39,305,257 (GRCm39)	missense	probably benign	0.33
R6590:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6690:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6925:Or8g32	UTSW	9	39,305,157 (GRCm39)	missense	probably benign	0.01
R6925:Or8g32	UTSW	9	39,305,156 (GRCm39)	missense	probably benign	0.32
R6982:Or8g32	UTSW	9	39,305,618 (GRCm39)	missense	probably damaging	1.00
R7662:Or8g32	UTSW	9	39,305,389 (GRCm39)	missense	probably benign	0.01
R8074:Or8g32	UTSW	9	39,305,242 (GRCm39)	missense	probably damaging	1.00
R8389:Or8g32	UTSW	9	39,305,912 (GRCm39)	missense	probably damaging	1.00
R9444:Or8g32	UTSW	9	39,305,365 (GRCm39)	missense	probably benign	0.19
R9642:Or8g32	UTSW	9	39,305,857 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGGCACTTTCACTCACTAATACAG -3'
(R):5'- GGGGTAACAACTGTGGACTG -3'

Sequencing Primer
(F):5'- TGAAGTGCAATCTTAACTTCTAAGC -3'
(R):5'- GACCAAAGTCAATGAAGGACAAACTG -3'

Posted On

2014-07-14