Mutagenetix > Incidental Mutation

Incidental Mutation 'R0125:Prkdc'

21329

Institutional Source

Beutler Lab

Gene Symbol

Prkdc

Ensembl Gene

ENSMUSG00000022672

Gene Name

protein kinase, DNA activated, catalytic polypeptide

Synonyms

slip, DOXNPH, DNA-PKcs, XRCC7, dxnph, DNA-PK, DNAPDcs

MMRRC Submission

038410-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.955) question?

Stock #

R0125 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

15637866-15842235 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 15699007 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 1082 (I1082F)

Ref Sequence

ENSEMBL: ENSMUSP00000023352 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023352]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000023352
AA Change: I1082F

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000023352
Gene: ENSMUSG00000022672
AA Change: I1082F

Domain	Start	End	E-Value	Type
low complexity region	125	138	N/A	INTRINSIC
low complexity region	1253	1263	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
NUC194	1810	2206	2.37e-246	SMART
SCOP:d1gw5a_	2210	2493	5e-3	SMART
low complexity region	2669	2681	N/A	INTRINSIC
low complexity region	2841	2855	N/A	INTRINSIC
Pfam:FAT	3024	3470	8.2e-75	PFAM
PI3Kc	3749	4068	3.67e-86	SMART
FATC	4096	4128	1.57e-9	SMART

Meta Mutation Damage Score

0.1080

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 94.7%
20x: 87.1%

Validation Efficiency

98% (96/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
PHENOTYPE: Mutations at this locus effect genome stability, radiation sensitivity and DNA repair. Nonsense (scid) and null homozygotes have severe combined immunodeficiency. A BALB/c variant allele reduces enzyme activity and predisposes to breast cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	T	C	14: 70,156,647 (GRCm38)		probably benign	Het
Adam23	T	C	1: 63,534,356 (GRCm38)	L261P	probably benign	Het
Adgra3	G	A	5: 50,001,852 (GRCm38)		probably benign	Het
Agtr1b	A	G	3: 20,315,540 (GRCm38)	F301L	probably benign	Het
Ahnak2	G	A	12: 112,785,156 (GRCm38)	T357I	probably benign	Het
Aldh1a7	T	C	19: 20,727,066 (GRCm38)		probably benign	Het
Apoh	A	T	11: 108,412,073 (GRCm38)	N288I	probably damaging	Het
Arfgap3	A	G	15: 83,343,139 (GRCm38)	V24A	probably benign	Het
Atp6v0a1	A	G	11: 101,038,851 (GRCm38)		probably null	Het
Axl	A	T	7: 25,786,943 (GRCm38)	M112K	probably benign	Het
Bnc2	A	C	4: 84,292,932 (GRCm38)	I425S	probably damaging	Het
Ccn4	T	C	15: 66,917,345 (GRCm38)	S227P	possibly damaging	Het
Cdc42bpa	C	T	1: 179,961,198 (GRCm38)	T30M	probably damaging	Het
Cebpz	C	A	17: 78,919,888 (GRCm38)	R1051M	possibly damaging	Het
Ces1d	A	C	8: 93,175,182 (GRCm38)		probably benign	Het
Chd1l	T	C	3: 97,587,149 (GRCm38)	N405S	probably benign	Het
Chodl	G	T	16: 78,941,423 (GRCm38)	G93V	probably damaging	Het
Cpeb2	C	T	5: 43,238,400 (GRCm38)		probably benign	Het
Crebbp	A	G	16: 4,117,241 (GRCm38)		probably benign	Het
Crybb3	T	C	5: 113,079,809 (GRCm38)	T49A	possibly damaging	Het
Ctps1	A	G	4: 120,561,525 (GRCm38)		probably benign	Het
Cyp26b1	A	G	6: 84,574,515 (GRCm38)	Y240H	probably damaging	Het
Cyp2d11	A	C	15: 82,389,221 (GRCm38)	V483G	probably benign	Het
Dnah14	A	T	1: 181,752,063 (GRCm38)	N3054Y	probably damaging	Het
Dspp	A	C	5: 104,178,039 (GRCm38)	D756A	unknown	Het
Dst	T	C	1: 34,270,903 (GRCm38)	S1553P	probably damaging	Het
Elp4	A	G	2: 105,792,214 (GRCm38)		probably null	Het
Eml6	G	T	11: 29,882,088 (GRCm38)	T194K	probably benign	Het
Evi5	A	G	5: 107,795,772 (GRCm38)	I569T	probably benign	Het
Fancm	C	T	12: 65,121,956 (GRCm38)	P1698S	possibly damaging	Het
Fhdc1	T	C	3: 84,445,545 (GRCm38)	D791G	probably benign	Het
Frem1	A	G	4: 83,011,951 (GRCm38)	Y253H	probably damaging	Het
Gpn3	A	G	5: 122,381,418 (GRCm38)	Y196C	probably benign	Het
Hcls1	A	G	16: 36,962,163 (GRCm38)	D398G	probably benign	Het
Hydin	T	C	8: 110,462,531 (GRCm38)	V1189A	probably benign	Het
Itgb3	G	A	11: 104,643,963 (GRCm38)	D549N	probably damaging	Het
Itpr2	A	G	6: 146,240,453 (GRCm38)	F1697S	probably benign	Het
Klk1b11	A	G	7: 43,999,051 (GRCm38)	T161A	probably benign	Het
Kntc1	G	A	5: 123,765,057 (GRCm38)		probably benign	Het
Map3k19	A	T	1: 127,823,100 (GRCm38)	F838Y	probably benign	Het
Map6	T	A	7: 99,335,980 (GRCm38)		probably null	Het
Mcrs1	A	G	15: 99,244,727 (GRCm38)		probably benign	Het
Mdn1	A	T	4: 32,729,956 (GRCm38)	Y2766F	probably damaging	Het
Med23	C	T	10: 24,900,788 (GRCm38)	H739Y	probably damaging	Het
Mmp17	T	A	5: 129,594,582 (GRCm38)	D65E	possibly damaging	Het
Mmp9	T	A	2: 164,951,257 (GRCm38)	L442Q	probably damaging	Het
Myo19	T	C	11: 84,888,175 (GRCm38)		probably benign	Het
Nedd1	A	C	10: 92,691,929 (GRCm38)	S468A	possibly damaging	Het
Niban2	T	A	2: 32,923,821 (GRCm38)	V682D	probably benign	Het
Nlrp4d	A	C	7: 10,382,389 (GRCm38)	V152G	probably damaging	Het
Nxf1	T	A	19: 8,762,806 (GRCm38)	D112E	probably benign	Het
Oas1h	A	T	5: 120,862,563 (GRCm38)	K79*	probably null	Het
Omg	T	A	11: 79,502,853 (GRCm38)	I60F	possibly damaging	Het
Or5p69	A	G	7: 108,368,369 (GRCm38)	Y293C	probably damaging	Het
Or8b101	A	G	9: 38,109,519 (GRCm38)	T278A	probably benign	Het
Or8b1b	T	A	9: 38,464,461 (GRCm38)	L140*	probably null	Het
Pck1	G	A	2: 173,156,081 (GRCm38)	W314*	probably null	Het
Pla2g15	T	C	8: 106,163,124 (GRCm38)	Y343H	probably benign	Het
Plcb3	T	C	19: 6,958,908 (GRCm38)	E749G	probably damaging	Het
Plgrkt	A	G	19: 29,351,042 (GRCm38)		probably null	Het
Pprc1	A	G	19: 46,069,512 (GRCm38)		probably benign	Het
Rapgef6	T	A	11: 54,625,875 (GRCm38)	Y172*	probably null	Het
Ros1	G	T	10: 52,125,789 (GRCm38)	A1079D	probably benign	Het
Sap30	T	C	8: 57,485,511 (GRCm38)	E147G	probably null	Het
Sell	T	C	1: 164,072,105 (GRCm38)		probably benign	Het
Senp1	A	T	15: 98,048,231 (GRCm38)	D544E	probably damaging	Het
Shpk	G	A	11: 73,214,222 (GRCm38)		probably benign	Het
Slc35b1	A	T	11: 95,386,527 (GRCm38)	T74S	probably benign	Het
Slc6a3	T	A	13: 73,569,979 (GRCm38)		probably benign	Het
Slf1	T	C	13: 77,043,745 (GRCm38)	N990S	probably benign	Het
Smgc	A	G	15: 91,854,543 (GRCm38)		probably benign	Het
Snx19	T	A	9: 30,440,219 (GRCm38)	V861D	probably damaging	Het
Sprr2e	C	T	3: 92,352,978 (GRCm38)	P39S	unknown	Het
Sstr2	T	A	11: 113,624,477 (GRCm38)	M74K	probably damaging	Het
St5	T	C	7: 109,556,338 (GRCm38)	K402E	probably benign	Het
Svep1	T	C	4: 58,099,937 (GRCm38)		probably benign	Het
Tas2r143	A	G	6: 42,400,955 (GRCm38)	I240V	probably benign	Het
Tbrg1	T	C	9: 37,652,641 (GRCm38)	I233V	probably benign	Het
Tecpr1	G	T	5: 144,197,899 (GRCm38)	D1055E	probably damaging	Het
Thap2	A	T	10: 115,376,372 (GRCm38)		probably null	Het
Tinagl1	A	G	4: 130,166,308 (GRCm38)	Y388H	probably damaging	Het
Ttn	T	A	2: 76,755,552 (GRCm38)	Y21945F	probably damaging	Het
Ugt2b1	A	T	5: 86,926,102 (GRCm38)	W133R	probably benign	Het
Usp24	C	T	4: 106,397,299 (GRCm38)	P1491L	possibly damaging	Het
Utp15	A	G	13: 98,250,882 (GRCm38)	S395P	possibly damaging	Het
Vav1	T	A	17: 57,299,847 (GRCm38)	L254Q	probably damaging	Het
Vmn2r104	T	C	17: 20,029,807 (GRCm38)	Y734C	probably damaging	Het
Vps8	T	A	16: 21,470,154 (GRCm38)	V421E	probably benign	Het
Xkr7	G	T	2: 153,032,426 (GRCm38)	A138S	probably benign	Het

Other mutations in Prkdc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Prkdc	APN	16	15,697,226 (GRCm38)	missense	probably damaging	1.00
IGL00225:Prkdc	APN	16	15,809,644 (GRCm38)	missense	possibly damaging	0.64
IGL00481:Prkdc	APN	16	15,790,466 (GRCm38)	missense	probably benign	0.41
IGL00488:Prkdc	APN	16	15,775,847 (GRCm38)	splice site	probably null
IGL00489:Prkdc	APN	16	15,799,926 (GRCm38)	missense	possibly damaging	0.51
IGL00579:Prkdc	APN	16	15,664,239 (GRCm38)	missense	probably damaging	1.00
IGL00587:Prkdc	APN	16	15,652,358 (GRCm38)	splice site	probably benign
IGL00666:Prkdc	APN	16	15,736,835 (GRCm38)	missense	probably damaging	1.00
IGL00675:Prkdc	APN	16	15,787,158 (GRCm38)	missense	probably benign	0.05
IGL00708:Prkdc	APN	16	15,779,426 (GRCm38)	missense	probably damaging	0.97
IGL00725:Prkdc	APN	16	15,816,639 (GRCm38)	missense	probably benign	0.10
IGL00818:Prkdc	APN	16	15,759,754 (GRCm38)	missense	possibly damaging	0.92
IGL00917:Prkdc	APN	16	15,739,564 (GRCm38)	missense	probably damaging	0.98
IGL00990:Prkdc	APN	16	15,702,115 (GRCm38)	missense	probably benign	0.03
IGL01126:Prkdc	APN	16	15,669,321 (GRCm38)	missense	probably benign	0.01
IGL01141:Prkdc	APN	16	15,726,704 (GRCm38)	missense	probably damaging	0.99
IGL01306:Prkdc	APN	16	15,667,731 (GRCm38)	missense	possibly damaging	0.67
IGL01326:Prkdc	APN	16	15,829,692 (GRCm38)	missense	probably benign
IGL01335:Prkdc	APN	16	15,816,896 (GRCm38)	critical splice donor site	probably null
IGL01419:Prkdc	APN	16	15,835,166 (GRCm38)	missense	probably damaging	1.00
IGL01434:Prkdc	APN	16	15,713,587 (GRCm38)	missense	probably benign	0.00
IGL01554:Prkdc	APN	16	15,652,302 (GRCm38)	missense	probably benign	0.05
IGL01671:Prkdc	APN	16	15,667,745 (GRCm38)	missense	possibly damaging	0.90
IGL01871:Prkdc	APN	16	15,783,087 (GRCm38)	missense	probably benign	0.00
IGL01874:Prkdc	APN	16	15,734,994 (GRCm38)	missense	possibly damaging	0.89
IGL01930:Prkdc	APN	16	15,698,887 (GRCm38)	missense	probably damaging	1.00
IGL01984:Prkdc	APN	16	15,708,779 (GRCm38)	missense	probably benign
IGL02121:Prkdc	APN	16	15,717,184 (GRCm38)	missense	probably benign	0.18
IGL02152:Prkdc	APN	16	15,669,285 (GRCm38)	missense	probably benign	0.15
IGL02172:Prkdc	APN	16	15,809,759 (GRCm38)	missense	probably benign	0.10
IGL02336:Prkdc	APN	16	15,785,979 (GRCm38)	missense	probably benign	0.01
IGL02336:Prkdc	APN	16	15,785,978 (GRCm38)	missense	possibly damaging	0.47
IGL02393:Prkdc	APN	16	15,816,758 (GRCm38)	missense	probably benign	0.42
IGL02406:Prkdc	APN	16	15,670,535 (GRCm38)	missense	probably benign	0.00
IGL02500:Prkdc	APN	16	15,714,282 (GRCm38)	critical splice donor site	probably null
IGL02568:Prkdc	APN	16	15,726,542 (GRCm38)	missense	probably damaging	0.98
IGL02579:Prkdc	APN	16	15,670,601 (GRCm38)	missense	possibly damaging	0.83
IGL02652:Prkdc	APN	16	15,783,087 (GRCm38)	missense	probably benign	0.00
IGL02661:Prkdc	APN	16	15,769,825 (GRCm38)	missense	possibly damaging	0.92
IGL02685:Prkdc	APN	16	15,836,043 (GRCm38)	missense	possibly damaging	0.61
IGL02741:Prkdc	APN	16	15,752,726 (GRCm38)	splice site	probably benign
IGL02803:Prkdc	APN	16	15,833,666 (GRCm38)	splice site	probably benign
IGL02866:Prkdc	APN	16	15,831,327 (GRCm38)	missense	probably damaging	1.00
IGL02882:Prkdc	APN	16	15,651,519 (GRCm38)	nonsense	probably null
IGL02989:Prkdc	APN	16	15,800,016 (GRCm38)	missense	possibly damaging	0.67
IGL03053:Prkdc	APN	16	15,834,166 (GRCm38)	missense	probably benign	0.02
IGL03071:Prkdc	APN	16	15,799,984 (GRCm38)	missense	probably benign	0.01
IGL03091:Prkdc	APN	16	15,705,310 (GRCm38)	splice site	probably benign
IGL03100:Prkdc	APN	16	15,713,635 (GRCm38)	missense	probably benign	0.08
IGL03128:Prkdc	APN	16	15,700,744 (GRCm38)	splice site	probably benign
IGL03168:Prkdc	APN	16	15,834,166 (GRCm38)	missense	probably benign	0.02
IGL03204:Prkdc	APN	16	15,769,801 (GRCm38)	missense	probably benign	0.01
IGL03390:Prkdc	APN	16	15,670,626 (GRCm38)	nonsense	probably null
anhimid	UTSW	16	15,725,461 (GRCm38)	critical splice donor site	probably null
anhinga	UTSW	16	15,708,932 (GRCm38)	critical splice donor site	probably null
Bushtit	UTSW	16	15,752,764 (GRCm38)	missense	probably damaging	0.97
clover	UTSW	16	15,702,156 (GRCm38)	splice site	probably benign
crackle	UTSW	16	15,786,050 (GRCm38)	critical splice donor site	probably null
Daffy	UTSW	16	15,829,697 (GRCm38)	missense	possibly damaging	0.86
darter	UTSW	16	15,773,613 (GRCm38)	missense	possibly damaging	0.93
Elmer_fudd	UTSW	16	15,808,058 (GRCm38)	missense	probably benign	0.01
envenomation	UTSW	16	15,835,227 (GRCm38)	nonsense	probably null
hobgoblin	UTSW	16	15,815,986 (GRCm38)	missense	probably damaging	1.00
Incubus	UTSW	16	15,672,327 (GRCm38)	missense	probably damaging	1.00
liming	UTSW	16	15,752,829 (GRCm38)	nonsense	probably null
newt	UTSW	16	15,727,726 (GRCm38)	missense	probably benign	0.04
ornithorhynchus	UTSW	16	15,816,659 (GRCm38)	critical splice donor site	probably null
primitive	UTSW	16	15,835,158 (GRCm38)	frame shift	probably null
roadrunner	UTSW	16	15,833,887 (GRCm38)	missense	probably damaging	1.00
Schreier	UTSW	16	15,670,528 (GRCm38)	missense	probably benign	0.00
screamer	UTSW	16	15,831,282 (GRCm38)	nonsense	probably null
Screamer10	UTSW	16	15,768,025 (GRCm38)	missense	probably damaging	0.98
screamer2	UTSW	16	15,652,552 (GRCm38)	critical splice donor site	probably null
screamer3	UTSW	16	15,740,332 (GRCm38)	critical splice donor site	probably null
screamer4	UTSW	16	15,783,079 (GRCm38)	missense	probably benign	0.00
screamer5	UTSW	16	15,687,404 (GRCm38)	missense	probably benign
screamer6	UTSW	16	15,759,605 (GRCm38)	missense	probably damaging	1.00
screamer7	UTSW	16	15,654,817 (GRCm38)	splice site	probably null
Screamer8	UTSW	16	15,719,433 (GRCm38)	missense	probably benign	0.00
Screamer9	UTSW	16	15,734,922 (GRCm38)	missense	probably benign	0.01
Tweetie	UTSW	16	15,717,801 (GRCm38)	missense	probably damaging	1.00
updock	UTSW	16	15,795,094 (GRCm38)	missense	probably benign
ANU23:Prkdc	UTSW	16	15,667,731 (GRCm38)	missense	possibly damaging	0.67
R0008:Prkdc	UTSW	16	15,708,701 (GRCm38)	splice site	probably benign
R0018:Prkdc	UTSW	16	15,726,542 (GRCm38)	missense	probably benign	0.03
R0018:Prkdc	UTSW	16	15,726,542 (GRCm38)	missense	probably benign	0.03
R0069:Prkdc	UTSW	16	15,726,504 (GRCm38)	missense	probably benign	0.03
R0131:Prkdc	UTSW	16	15,713,653 (GRCm38)	missense	probably benign	0.09
R0131:Prkdc	UTSW	16	15,713,653 (GRCm38)	missense	probably benign	0.09
R0132:Prkdc	UTSW	16	15,713,653 (GRCm38)	missense	probably benign	0.09
R0137:Prkdc	UTSW	16	15,740,332 (GRCm38)	critical splice donor site	probably null
R0334:Prkdc	UTSW	16	15,736,799 (GRCm38)	missense	probably benign	0.00
R0373:Prkdc	UTSW	16	15,791,927 (GRCm38)	missense	probably damaging	1.00
R0485:Prkdc	UTSW	16	15,833,740 (GRCm38)	missense	probably damaging	0.97
R0511:Prkdc	UTSW	16	15,831,282 (GRCm38)	nonsense	probably null
R0538:Prkdc	UTSW	16	15,833,788 (GRCm38)	missense	probably damaging	1.00
R0595:Prkdc	UTSW	16	15,808,088 (GRCm38)	missense	probably damaging	1.00
R0607:Prkdc	UTSW	16	15,772,057 (GRCm38)	missense	probably damaging	0.98
R0616:Prkdc	UTSW	16	15,690,407 (GRCm38)	missense	probably damaging	1.00
R0630:Prkdc	UTSW	16	15,810,801 (GRCm38)	missense	probably damaging	1.00
R0694:Prkdc	UTSW	16	15,768,637 (GRCm38)	missense	probably damaging	1.00
R0702:Prkdc	UTSW	16	15,785,971 (GRCm38)	missense	possibly damaging	0.95
R0965:Prkdc	UTSW	16	15,829,716 (GRCm38)	missense	probably benign
R1027:Prkdc	UTSW	16	15,650,712 (GRCm38)	missense	possibly damaging	0.80
R1029:Prkdc	UTSW	16	15,654,749 (GRCm38)	splice site	probably benign
R1033:Prkdc	UTSW	16	15,767,951 (GRCm38)	missense	probably damaging	1.00
R1067:Prkdc	UTSW	16	15,752,782 (GRCm38)	missense	probably damaging	0.99
R1116:Prkdc	UTSW	16	15,783,079 (GRCm38)	missense	probably benign	0.00
R1187:Prkdc	UTSW	16	15,759,746 (GRCm38)	missense	probably damaging	0.98
R1226:Prkdc	UTSW	16	15,673,997 (GRCm38)	missense	possibly damaging	0.80
R1279:Prkdc	UTSW	16	15,690,282 (GRCm38)	missense	probably damaging	1.00
R1304:Prkdc	UTSW	16	15,759,723 (GRCm38)	missense	probably damaging	0.99
R1314:Prkdc	UTSW	16	15,664,227 (GRCm38)	missense	possibly damaging	0.68
R1351:Prkdc	UTSW	16	15,667,700 (GRCm38)	missense	possibly damaging	0.62
R1509:Prkdc	UTSW	16	15,731,566 (GRCm38)	missense	probably damaging	1.00
R1512:Prkdc	UTSW	16	15,687,404 (GRCm38)	missense	probably benign
R1531:Prkdc	UTSW	16	15,772,106 (GRCm38)	missense	probably benign	0.01
R1579:Prkdc	UTSW	16	15,675,328 (GRCm38)	missense	probably benign	0.00
R1669:Prkdc	UTSW	16	15,734,058 (GRCm38)	missense	probably damaging	1.00
R1682:Prkdc	UTSW	16	15,676,989 (GRCm38)	missense	probably benign	0.19
R1713:Prkdc	UTSW	16	15,795,094 (GRCm38)	missense	probably benign
R1762:Prkdc	UTSW	16	15,637,961 (GRCm38)	missense	probably benign
R1789:Prkdc	UTSW	16	15,739,524 (GRCm38)	missense	probably damaging	1.00
R1822:Prkdc	UTSW	16	15,759,605 (GRCm38)	missense	probably damaging	1.00
R1848:Prkdc	UTSW	16	15,808,058 (GRCm38)	missense	probably benign	0.01
R1887:Prkdc	UTSW	16	15,829,635 (GRCm38)	missense	probably benign	0.00
R1891:Prkdc	UTSW	16	15,725,436 (GRCm38)	missense	probably benign	0.02
R1921:Prkdc	UTSW	16	15,714,215 (GRCm38)	missense	possibly damaging	0.80
R1922:Prkdc	UTSW	16	15,714,266 (GRCm38)	missense	probably benign	0.00
R1929:Prkdc	UTSW	16	15,654,817 (GRCm38)	splice site	probably null
R1939:Prkdc	UTSW	16	15,835,913 (GRCm38)	missense	possibly damaging	0.95
R2021:Prkdc	UTSW	16	15,677,009 (GRCm38)	missense	probably benign	0.00
R2033:Prkdc	UTSW	16	15,687,352 (GRCm38)	splice site	probably benign
R2056:Prkdc	UTSW	16	15,727,605 (GRCm38)	missense	probably benign	0.03
R2057:Prkdc	UTSW	16	15,727,605 (GRCm38)	missense	probably benign	0.03
R2058:Prkdc	UTSW	16	15,727,605 (GRCm38)	missense	probably benign	0.03
R2082:Prkdc	UTSW	16	15,715,963 (GRCm38)	missense	probably damaging	1.00
R2109:Prkdc	UTSW	16	15,687,390 (GRCm38)	missense	probably benign	0.01
R2124:Prkdc	UTSW	16	15,719,433 (GRCm38)	missense	probably benign	0.00
R2164:Prkdc	UTSW	16	15,705,207 (GRCm38)	missense	probably damaging	1.00
R2174:Prkdc	UTSW	16	15,734,922 (GRCm38)	missense	probably benign	0.01
R2191:Prkdc	UTSW	16	15,698,824 (GRCm38)	missense	probably damaging	1.00
R2270:Prkdc	UTSW	16	15,654,817 (GRCm38)	splice site	probably null
R2271:Prkdc	UTSW	16	15,654,817 (GRCm38)	splice site	probably null
R2272:Prkdc	UTSW	16	15,654,817 (GRCm38)	splice site	probably null
R2356:Prkdc	UTSW	16	15,684,204 (GRCm38)	missense	probably benign
R2852:Prkdc	UTSW	16	15,652,552 (GRCm38)	critical splice donor site	probably null
R3115:Prkdc	UTSW	16	15,664,358 (GRCm38)	missense	probably benign	0.01
R3116:Prkdc	UTSW	16	15,664,358 (GRCm38)	missense	probably benign	0.01
R3499:Prkdc	UTSW	16	15,768,025 (GRCm38)	missense	probably damaging	0.98
R3687:Prkdc	UTSW	16	15,799,967 (GRCm38)	missense	probably benign
R3834:Prkdc	UTSW	16	15,791,946 (GRCm38)	missense	probably damaging	1.00
R3835:Prkdc	UTSW	16	15,791,946 (GRCm38)	missense	probably damaging	1.00
R3961:Prkdc	UTSW	16	15,829,611 (GRCm38)	splice site	probably null
R4151:Prkdc	UTSW	16	15,816,773 (GRCm38)	missense	probably benign
R4233:Prkdc	UTSW	16	15,835,919 (GRCm38)	missense	probably benign	0.11
R4281:Prkdc	UTSW	16	15,806,099 (GRCm38)	splice site	probably null
R4296:Prkdc	UTSW	16	15,737,905 (GRCm38)	missense	probably damaging	0.99
R4344:Prkdc	UTSW	16	15,768,022 (GRCm38)	missense	probably damaging	0.98
R4424:Prkdc	UTSW	16	15,836,082 (GRCm38)	missense	probably damaging	1.00
R4424:Prkdc	UTSW	16	15,773,739 (GRCm38)	missense	probably damaging	0.98
R4497:Prkdc	UTSW	16	15,700,653 (GRCm38)	missense	probably benign	0.43
R4549:Prkdc	UTSW	16	15,736,870 (GRCm38)	missense	possibly damaging	0.89
R4594:Prkdc	UTSW	16	15,767,966 (GRCm38)	missense	possibly damaging	0.64
R4603:Prkdc	UTSW	16	15,810,824 (GRCm38)	missense	probably damaging	0.98
R4615:Prkdc	UTSW	16	15,663,074 (GRCm38)	missense	probably damaging	0.99
R4648:Prkdc	UTSW	16	15,816,774 (GRCm38)	missense	probably benign	0.05
R4662:Prkdc	UTSW	16	15,734,052 (GRCm38)	missense	probably damaging	1.00
R4680:Prkdc	UTSW	16	15,772,030 (GRCm38)	missense	probably benign	0.00
R4700:Prkdc	UTSW	16	15,702,112 (GRCm38)	missense	probably damaging	1.00
R4716:Prkdc	UTSW	16	15,810,837 (GRCm38)	missense	probably benign	0.32
R4720:Prkdc	UTSW	16	15,667,715 (GRCm38)	missense	probably benign
R4785:Prkdc	UTSW	16	15,648,976 (GRCm38)	missense	probably benign	0.21
R4822:Prkdc	UTSW	16	15,650,712 (GRCm38)	missense	possibly damaging	0.80
R4829:Prkdc	UTSW	16	15,702,075 (GRCm38)	missense	possibly damaging	0.80
R4981:Prkdc	UTSW	16	15,678,309 (GRCm38)	missense	probably damaging	1.00
R4989:Prkdc	UTSW	16	15,673,997 (GRCm38)	missense	possibly damaging	0.80
R5059:Prkdc	UTSW	16	15,838,018 (GRCm38)	missense	probably damaging	1.00
R5074:Prkdc	UTSW	16	15,772,048 (GRCm38)	missense	probably damaging	1.00
R5115:Prkdc	UTSW	16	15,790,580 (GRCm38)	missense	probably benign
R5151:Prkdc	UTSW	16	15,716,035 (GRCm38)	missense	probably damaging	1.00
R5165:Prkdc	UTSW	16	15,678,272 (GRCm38)	missense	probably damaging	1.00
R5215:Prkdc	UTSW	16	15,772,121 (GRCm38)	missense	possibly damaging	0.64
R5270:Prkdc	UTSW	16	15,734,955 (GRCm38)	missense	probably damaging	1.00
R5278:Prkdc	UTSW	16	15,714,974 (GRCm38)	missense	probably damaging	1.00
R5351:Prkdc	UTSW	16	15,831,312 (GRCm38)	missense	probably benign	0.03
R5416:Prkdc	UTSW	16	15,805,950 (GRCm38)	missense	probably damaging	1.00
R5418:Prkdc	UTSW	16	15,795,097 (GRCm38)	missense	probably benign	0.20
R5437:Prkdc	UTSW	16	15,769,875 (GRCm38)	missense	possibly damaging	0.46
R5452:Prkdc	UTSW	16	15,768,637 (GRCm38)	missense	possibly damaging	0.96
R5518:Prkdc	UTSW	16	15,678,308 (GRCm38)	missense	probably damaging	1.00
R5538:Prkdc	UTSW	16	15,651,469 (GRCm38)	missense	probably damaging	1.00
R5589:Prkdc	UTSW	16	15,706,791 (GRCm38)	missense	probably benign	0.02
R5618:Prkdc	UTSW	16	15,809,612 (GRCm38)	missense	probably damaging	1.00
R5640:Prkdc	UTSW	16	15,829,769 (GRCm38)	missense	possibly damaging	0.86
R5661:Prkdc	UTSW	16	15,810,770 (GRCm38)	missense	possibly damaging	0.81
R5771:Prkdc	UTSW	16	15,664,233 (GRCm38)	missense	probably damaging	1.00
R5772:Prkdc	UTSW	16	15,779,388 (GRCm38)	missense	possibly damaging	0.49
R5783:Prkdc	UTSW	16	15,717,801 (GRCm38)	missense	probably damaging	1.00
R5792:Prkdc	UTSW	16	15,816,752 (GRCm38)	missense	probably damaging	1.00
R5797:Prkdc	UTSW	16	15,737,834 (GRCm38)	nonsense	probably null
R5826:Prkdc	UTSW	16	15,734,098 (GRCm38)	missense	probably benign
R5883:Prkdc	UTSW	16	15,715,914 (GRCm38)	missense	probably benign
R5895:Prkdc	UTSW	16	15,752,829 (GRCm38)	nonsense	probably null
R5998:Prkdc	UTSW	16	15,783,157 (GRCm38)	missense	probably damaging	1.00
R6000:Prkdc	UTSW	16	15,829,697 (GRCm38)	missense	possibly damaging	0.86
R6120:Prkdc	UTSW	16	15,739,471 (GRCm38)	missense	probably benign	0.00
R6145:Prkdc	UTSW	16	15,772,073 (GRCm38)	missense	probably damaging	1.00
R6209:Prkdc	UTSW	16	15,790,592 (GRCm38)	missense	probably damaging	1.00
R6293:Prkdc	UTSW	16	15,787,155 (GRCm38)	missense	probably benign	0.00
R6321:Prkdc	UTSW	16	15,714,919 (GRCm38)	missense	probably benign
R6376:Prkdc	UTSW	16	15,769,885 (GRCm38)	missense	probably benign	0.06
R6387:Prkdc	UTSW	16	15,698,815 (GRCm38)	missense	probably benign	0.01
R6406:Prkdc	UTSW	16	15,717,801 (GRCm38)	missense	probably damaging	1.00
R6469:Prkdc	UTSW	16	15,795,075 (GRCm38)	missense	probably benign	0.10
R6486:Prkdc	UTSW	16	15,752,764 (GRCm38)	missense	probably damaging	0.97
R6665:Prkdc	UTSW	16	15,786,050 (GRCm38)	critical splice donor site	probably null
R6703:Prkdc	UTSW	16	15,670,528 (GRCm38)	missense	probably benign	0.00
R6774:Prkdc	UTSW	16	15,725,461 (GRCm38)	critical splice donor site	probably null
R6854:Prkdc	UTSW	16	15,651,538 (GRCm38)	missense	probably damaging	1.00
R6878:Prkdc	UTSW	16	15,777,072 (GRCm38)	missense	probably benign	0.31
R6882:Prkdc	UTSW	16	15,808,156 (GRCm38)	missense	probably benign	0.33
R6882:Prkdc	UTSW	16	15,783,263 (GRCm38)	critical splice donor site	probably null
R6949:Prkdc	UTSW	16	15,799,989 (GRCm38)	missense	probably benign
R6950:Prkdc	UTSW	16	15,815,986 (GRCm38)	missense	probably damaging	1.00
R7019:Prkdc	UTSW	16	15,769,966 (GRCm38)	missense	probably benign	0.00
R7064:Prkdc	UTSW	16	15,790,453 (GRCm38)	missense	probably benign	0.00
R7097:Prkdc	UTSW	16	15,689,343 (GRCm38)	missense	probably damaging	1.00
R7201:Prkdc	UTSW	16	15,698,803 (GRCm38)	missense	probably benign	0.12
R7235:Prkdc	UTSW	16	15,714,263 (GRCm38)	missense	probably benign
R7283:Prkdc	UTSW	16	15,717,764 (GRCm38)	missense	probably benign	0.00
R7401:Prkdc	UTSW	16	15,648,738 (GRCm38)	missense	probably damaging	1.00
R7525:Prkdc	UTSW	16	15,672,327 (GRCm38)	missense	probably damaging	1.00
R7647:Prkdc	UTSW	16	15,737,943 (GRCm38)	missense	probably damaging	1.00
R7679:Prkdc	UTSW	16	15,831,319 (GRCm38)	missense	probably damaging	1.00
R7803:Prkdc	UTSW	16	15,806,096 (GRCm38)	missense	probably null	0.05
R7858:Prkdc	UTSW	16	15,689,277 (GRCm38)	missense	probably benign	0.11
R7872:Prkdc	UTSW	16	15,715,006 (GRCm38)	missense	probably benign	0.05
R7896:Prkdc	UTSW	16	15,708,903 (GRCm38)	missense	probably damaging	0.97
R8032:Prkdc	UTSW	16	15,779,451 (GRCm38)	missense	probably benign	0.00
R8055:Prkdc	UTSW	16	15,816,885 (GRCm38)	missense	probably benign	0.09
R8153:Prkdc	UTSW	16	15,664,244 (GRCm38)	missense	probably damaging	1.00
R8281:Prkdc	UTSW	16	15,705,253 (GRCm38)	missense	probably damaging	1.00
R8302:Prkdc	UTSW	16	15,836,082 (GRCm38)	missense	probably damaging	1.00
R8322:Prkdc	UTSW	16	15,714,141 (GRCm38)	splice site	probably benign
R8401:Prkdc	UTSW	16	15,773,613 (GRCm38)	missense	possibly damaging	0.93
R8440:Prkdc	UTSW	16	15,835,158 (GRCm38)	frame shift	probably null
R8458:Prkdc	UTSW	16	15,790,676 (GRCm38)	critical splice donor site	probably null
R8472:Prkdc	UTSW	16	15,651,536 (GRCm38)	missense	probably damaging	1.00
R8478:Prkdc	UTSW	16	15,648,924 (GRCm38)	missense	probably benign	0.00
R8515:Prkdc	UTSW	16	15,664,368 (GRCm38)	missense	probably damaging	1.00
R8546:Prkdc	UTSW	16	15,663,035 (GRCm38)	missense	probably damaging	1.00
R8678:Prkdc	UTSW	16	15,708,932 (GRCm38)	critical splice donor site	probably null
R8739:Prkdc	UTSW	16	15,808,204 (GRCm38)	missense	probably benign	0.01
R8749:Prkdc	UTSW	16	15,783,165 (GRCm38)	missense	possibly damaging	0.85
R8836:Prkdc	UTSW	16	15,727,659 (GRCm38)	missense	probably damaging	1.00
R8904:Prkdc	UTSW	16	15,727,726 (GRCm38)	missense	probably benign	0.04
R8952:Prkdc	UTSW	16	15,673,760 (GRCm38)	intron	probably benign
R8971:Prkdc	UTSW	16	15,675,365 (GRCm38)	missense	probably null	0.99
R8974:Prkdc	UTSW	16	15,799,862 (GRCm38)	splice site	probably null
R9052:Prkdc	UTSW	16	15,690,296 (GRCm38)	missense	probably benign	0.05
R9069:Prkdc	UTSW	16	15,835,227 (GRCm38)	nonsense	probably null
R9200:Prkdc	UTSW	16	15,705,289 (GRCm38)	missense	probably damaging	1.00
R9235:Prkdc	UTSW	16	15,833,887 (GRCm38)	missense	probably damaging	1.00
R9278:Prkdc	UTSW	16	15,816,659 (GRCm38)	critical splice donor site	probably null
R9309:Prkdc	UTSW	16	15,708,928 (GRCm38)	nonsense	probably null
R9386:Prkdc	UTSW	16	15,678,272 (GRCm38)	missense	probably damaging	0.99
R9452:Prkdc	UTSW	16	15,667,601 (GRCm38)	missense	possibly damaging	0.90
R9500:Prkdc	UTSW	16	15,839,215 (GRCm38)	missense	possibly damaging	0.76
R9608:Prkdc	UTSW	16	15,730,471 (GRCm38)	missense	probably damaging	1.00
R9608:Prkdc	UTSW	16	15,730,470 (GRCm38)	missense	possibly damaging	0.96
R9636:Prkdc	UTSW	16	15,730,477 (GRCm38)	missense	probably benign	0.19
R9656:Prkdc	UTSW	16	15,799,954 (GRCm38)	missense	probably benign	0.00
R9674:Prkdc	UTSW	16	15,715,955 (GRCm38)	missense	probably damaging	0.98
R9760:Prkdc	UTSW	16	15,839,180 (GRCm38)	nonsense	probably null
X0023:Prkdc	UTSW	16	15,740,278 (GRCm38)	missense	probably benign
Z1176:Prkdc	UTSW	16	15,687,422 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCAGTTACCACAATGAAGCTGTCC -3'
(R):5'- GGTCCATCTCTGACAAGTGTTCACG -3'

Sequencing Primer
(F):5'- ACAATGAAGCTGTCCTTGTCTTG -3'
(R):5'- CTCTGACAAGTGTTCACGAACTTAC -3'

Posted On

2013-04-11