Incidental Mutation 'R1941:Pga5'
ID214043
Institutional Source Beutler Lab
Gene Symbol Pga5
Ensembl Gene ENSMUSG00000024738
Gene Namepepsinogen 5, group I
Synonyms1110035E17Rik, Pepf, pepsinogen A5
MMRRC Submission 039959-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.076) question?
Stock #R1941 (G1)
Quality Score225
Status Not validated
Chromosome19
Chromosomal Location10668956-10678071 bp(-) (GRCm38)
Type of Mutationsplice site
DNA Base Change (assembly) G to T at 10669456 bp
ZygosityHeterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000025647 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025647] [ENSMUST00000025647] [ENSMUST00000055115]
Predicted Effect probably null
Transcript: ENSMUST00000025647
SMART Domains Protein: ENSMUSP00000025647
Gene: ENSMUSG00000024738

DomainStartEndE-ValueType
Pfam:A1_Propeptide 16 44 1.1e-13 PFAM
Pfam:Asp 73 386 1.1e-112 PFAM
Pfam:TAXi_N 74 229 7.6e-12 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000025647
SMART Domains Protein: ENSMUSP00000025647
Gene: ENSMUSG00000024738

DomainStartEndE-ValueType
Pfam:A1_Propeptide 16 44 1.1e-13 PFAM
Pfam:Asp 73 386 1.1e-112 PFAM
Pfam:TAXi_N 74 229 7.6e-12 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000055115
SMART Domains Protein: ENSMUSP00000056958
Gene: ENSMUSG00000043789

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
EGF 70 98 2.2e1 SMART
EGF_CA 142 180 6.91e-9 SMART
EGF_CA 181 219 7.75e-12 SMART
EGF_CA 220 262 1.11e-12 SMART
low complexity region 294 312 N/A INTRINSIC
low complexity region 335 353 N/A INTRINSIC
VWC 378 432 2.91e-6 SMART
VWC 435 488 4.58e-4 SMART
VWC 493 551 2.06e-6 SMART
VWC 560 617 9.74e-8 SMART
VWC 621 676 1.35e-10 SMART
VWC 679 725 2.58e-1 SMART
low complexity region 761 772 N/A INTRINSIC
low complexity region 889 903 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000225016
Predicted Effect noncoding transcript
Transcript: ENSMUST00000225135
Coding Region Coverage
  • 1x: 97.3%
  • 3x: 96.6%
  • 10x: 94.8%
  • 20x: 91.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Art2b A C 7: 101,580,317 F125C probably damaging Het
BC027072 T G 17: 71,752,068 T205P probably damaging Het
Camta1 T C 4: 151,075,155 Y1616C probably damaging Het
Cep162 T G 9: 87,199,995 I1286L probably benign Het
Chd9 T A 8: 90,977,069 probably null Het
Col11a2 T A 17: 34,044,951 S247T probably benign Het
Col6a4 T G 9: 106,075,010 D563A probably benign Het
Crebbp C T 16: 4,179,691 M176I probably benign Het
Cyp4a12b A G 4: 115,438,059 N454S probably damaging Het
Dhx8 T C 11: 101,752,198 probably null Het
Dock7 G A 4: 98,984,715 L1165F probably benign Het
Fam117a A T 11: 95,380,798 N399Y probably damaging Het
Fmn1 A G 2: 113,365,143 K396R unknown Het
Foxk1 T A 5: 142,456,674 V693E possibly damaging Het
Gml A G 15: 74,817,171 L10P probably damaging Het
Gpx8 A G 13: 113,046,275 L34P probably damaging Het
Kank2 A G 9: 21,772,866 L689P possibly damaging Het
Mt4 G A 8: 94,138,246 C16Y possibly damaging Het
Naa15 C A 3: 51,455,934 Y346* probably null Het
Ntrk3 C T 7: 78,247,262 V676I probably damaging Het
Oasl2 G A 5: 114,911,362 probably benign Het
Olfr376 T A 11: 73,375,621 F294I probably damaging Het
Olfr767 T G 10: 129,079,954 N3T probably damaging Het
Olfr811 T A 10: 129,802,412 M38L probably benign Het
Olfr835 A C 9: 19,035,866 T248P possibly damaging Het
Olfr860 G A 9: 19,845,950 S223F probably damaging Het
Otud7a C T 7: 63,729,826 R273* probably null Het
Pde11a C T 2: 76,291,250 R329Q probably benign Het
Ppp1r1a A G 15: 103,533,101 probably null Het
Pus10 A G 11: 23,711,198 Q262R possibly damaging Het
Rbm17 T C 2: 11,589,074 K241R possibly damaging Het
Rp1l1 T G 14: 64,022,252 D114E probably damaging Het
Safb T A 17: 56,598,992 probably benign Het
Sgpl1 T C 10: 61,103,307 Y371C probably damaging Het
Siglec1 C A 2: 131,078,131 A827S possibly damaging Het
Smg5 T A 3: 88,345,380 S158T possibly damaging Het
Sp2 C T 11: 96,955,936 C527Y probably damaging Het
Spaca6 A G 17: 17,838,402 I71V probably benign Het
Spaca6 A G 17: 17,838,430 E80G probably damaging Het
Steap1 C A 5: 5,740,541 A136S probably damaging Het
Supv3l1 G C 10: 62,449,612 P25R probably benign Het
Tex21 T C 12: 76,221,684 K108R possibly damaging Het
Tnc A G 4: 64,014,964 Y688H probably damaging Het
Tnr G A 1: 159,850,134 E30K possibly damaging Het
Tnrc18 G T 5: 142,815,150 P18T probably damaging Het
Ttk T G 9: 83,853,126 S422A probably benign Het
Ubap1 A G 4: 41,378,968 K61E probably damaging Het
Ubr2 C T 17: 46,974,026 R522H probably damaging Het
Vwf A T 6: 125,639,279 E1185D possibly damaging Het
Wwp2 A G 8: 107,517,915 T194A probably benign Het
Zfp36 A G 7: 28,377,646 V279A probably damaging Het
Zfp870 C A 17: 32,882,804 R517L possibly damaging Het
Other mutations in Pga5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01660:Pga5 APN 19 10675092 missense probably damaging 1.00
IGL02037:Pga5 APN 19 10676701 missense probably benign 0.01
IGL02069:Pga5 APN 19 10669399 missense possibly damaging 0.76
IGL02197:Pga5 APN 19 10671913 splice site probably benign
IGL02871:Pga5 APN 19 10671780 splice site probably benign
R0238:Pga5 UTSW 19 10669453 missense probably damaging 1.00
R0238:Pga5 UTSW 19 10669453 missense probably damaging 1.00
R0239:Pga5 UTSW 19 10669453 missense probably damaging 1.00
R0239:Pga5 UTSW 19 10669453 missense probably damaging 1.00
R1573:Pga5 UTSW 19 10673837 missense probably benign 0.13
R4354:Pga5 UTSW 19 10674826 critical splice donor site probably null
R4568:Pga5 UTSW 19 10671852 missense probably damaging 1.00
R5119:Pga5 UTSW 19 10676689 missense probably benign 0.00
R5738:Pga5 UTSW 19 10669660 missense probably benign 0.05
R5864:Pga5 UTSW 19 10675149 missense probably damaging 1.00
R6176:Pga5 UTSW 19 10671785 splice site probably null
R6270:Pga5 UTSW 19 10674861 missense probably benign
R6990:Pga5 UTSW 19 10669415 missense probably benign 0.03
Z1176:Pga5 UTSW 19 10669159 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCATGCCCTCCTCAAAGTTG -3'
(R):5'- TTATGGACACAGGGACCTCC -3'

Sequencing Primer
(F):5'- GCAATTGTGTGACCGATCC -3'
(R):5'- ACAGGGACCTCCTTGCTGAC -3'
Posted On2014-07-14