Mutagenetix > Incidental Mutation

Incidental Mutation 'R0128:Hectd4'

21500

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

038413-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.927) question?

Stock #

R0128 (G1)

Quality Score

203

Status

Validated (trace)

Chromosome

Chromosomal Location

121358282-121506640 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121487306 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 3434 (Y3434H)

Ref Sequence

ENSEMBL: ENSMUSP00000048345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042614
AA Change: Y3434H

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: Y3434H

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Meta Mutation Damage Score

0.0606

Coding Region Coverage

1x: 99.0%
3x: 97.6%
10x: 93.0%
20x: 79.3%

Validation Efficiency

99% (84/85)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	G	6: 128,552,602 (GRCm39)		probably benign	Het
Abcd4	T	G	12: 84,659,126 (GRCm39)	Q210P	possibly damaging	Het
Ablim2	G	A	5: 35,966,520 (GRCm39)		probably benign	Het
Acte1	G	T	7: 143,445,492 (GRCm39)	G178C	probably damaging	Het
Actl6b	A	G	5: 137,553,327 (GRCm39)	N113S	probably benign	Het
Actn3	A	T	19: 4,921,643 (GRCm39)	V179E	probably damaging	Het
Aff4	C	A	11: 53,306,293 (GRCm39)	T1145N	probably damaging	Het
Ankrd42	G	A	7: 92,241,067 (GRCm39)	Q431*	probably null	Het
Anxa9	A	G	3: 95,209,733 (GRCm39)	S129P	probably benign	Het
Arfgef2	T	G	2: 166,677,639 (GRCm39)	I88S	probably damaging	Het
Asap3	C	A	4: 135,961,915 (GRCm39)	N285K	probably damaging	Het
Atp6v0a2	A	G	5: 124,790,248 (GRCm39)	N477S	probably damaging	Het
Atp7b	C	T	8: 22,518,188 (GRCm39)	E205K	possibly damaging	Het
Atp8b5	T	A	4: 43,369,715 (GRCm39)		probably null	Het
C87436	G	A	6: 86,446,809 (GRCm39)	G533D	probably damaging	Het
Ccdc138	T	A	10: 58,364,182 (GRCm39)	I314N	probably damaging	Het
Ccs	A	G	19: 4,875,654 (GRCm39)	F237S	probably damaging	Het
Ccz1	T	G	5: 143,946,112 (GRCm39)		probably benign	Het
Cdcp2	C	T	4: 106,963,904 (GRCm39)		probably benign	Het
Chd1	A	G	17: 17,613,829 (GRCm39)	N531S	probably damaging	Het
Clptm1	A	T	7: 19,368,932 (GRCm39)	F476I	probably damaging	Het
Colec12	C	T	18: 9,858,921 (GRCm39)	P568L	unknown	Het
Cped1	T	A	6: 22,121,038 (GRCm39)	Y373N	probably benign	Het
Cr2	A	T	1: 194,848,539 (GRCm39)	V328D	probably damaging	Het
D630045J12Rik	A	T	6: 38,126,706 (GRCm39)		probably benign	Het
Dcdc2a	A	T	13: 25,371,655 (GRCm39)		probably benign	Het
Dlg1	G	T	16: 31,676,883 (GRCm39)		probably null	Het
Epb41l5	A	C	1: 119,477,632 (GRCm39)	V705G	possibly damaging	Het
Ergic3	C	A	2: 155,853,060 (GRCm39)	R43S	possibly damaging	Het
Flnb	T	C	14: 7,901,951 (GRCm38)	V938A	probably damaging	Het
Frmd4a	T	C	2: 4,608,903 (GRCm39)	Y928H	probably damaging	Het
Fyn	C	T	10: 39,387,978 (GRCm39)	T78M	probably benign	Het
Gdap2	A	G	3: 100,109,311 (GRCm39)	T443A	probably damaging	Het
Ghrl	A	T	6: 113,694,129 (GRCm39)		probably benign	Het
Gm4787	T	A	12: 81,424,521 (GRCm39)	K546*	probably null	Het
Gm6576	C	G	15: 27,026,086 (GRCm39)		noncoding transcript	Het
Got1	C	T	19: 43,512,816 (GRCm39)	D27N	probably benign	Het
Gucy2c	C	T	6: 136,681,247 (GRCm39)	V946I	probably damaging	Het
Hp1bp3	C	T	4: 137,964,520 (GRCm39)	S348F	probably damaging	Het
Itpr1	A	G	6: 108,448,170 (GRCm39)		probably benign	Het
Kctd1	G	A	18: 15,107,237 (GRCm39)	P743S	probably benign	Het
Klhl23	T	C	2: 69,664,310 (GRCm39)	V553A	probably damaging	Het
Krt24	T	C	11: 99,171,093 (GRCm39)	D495G	probably damaging	Het
L3hypdh	C	T	12: 72,123,917 (GRCm39)		probably null	Het
Lipo3	C	T	19: 33,534,506 (GRCm39)		probably null	Het
Lman2l	G	T	1: 36,463,945 (GRCm39)	S171*	probably null	Het
Lrp1b	T	C	2: 41,401,520 (GRCm39)	D378G	probably damaging	Het
Map3k4	T	A	17: 12,466,950 (GRCm39)	D1104V	probably damaging	Het
Mpeg1	T	C	19: 12,438,587 (GRCm39)	V15A	probably benign	Het
Narf	C	T	11: 121,141,662 (GRCm39)	R356C	probably damaging	Het
Nebl	T	A	2: 17,397,834 (GRCm39)	Q487H	possibly damaging	Het
Olfm5	G	A	7: 103,810,133 (GRCm39)	A76V	probably benign	Het
Or1j11	T	A	2: 36,312,299 (GRCm39)	D296E	probably benign	Het
Or2z8	C	T	8: 72,812,244 (GRCm39)	T240M	probably damaging	Het
Or52p1	A	T	7: 104,267,788 (GRCm39)	I301F	probably damaging	Het
Or5ak22	T	A	2: 85,230,305 (GRCm39)	S191C	probably damaging	Het
Or8k40	T	C	2: 86,584,231 (GRCm39)	M284V	probably benign	Het
Palb2	A	T	7: 121,727,389 (GRCm39)	Y160*	probably null	Het
Pasd1	T	C	X: 70,983,161 (GRCm39)	C378R	possibly damaging	Het
Paxip1	C	T	5: 27,949,183 (GRCm39)		probably benign	Het
Pclo	A	G	5: 14,729,811 (GRCm39)		probably benign	Het
Pdcd11	G	A	19: 47,108,301 (GRCm39)	V1223I	probably benign	Het
Pde6c	T	C	19: 38,157,813 (GRCm39)		probably benign	Het
Prr12	A	G	7: 44,699,463 (GRCm39)		probably benign	Het
Prss39	T	A	1: 34,541,281 (GRCm39)		probably benign	Het
Samd5	A	G	10: 9,550,683 (GRCm39)	W9R	probably damaging	Het
Sfr1	A	G	19: 47,723,457 (GRCm39)	*320W	probably null	Het
Sft2d1rt	A	G	11: 45,943,120 (GRCm39)	M1T	probably null	Het
Sh3bp4	A	G	1: 89,073,036 (GRCm39)	N628S	possibly damaging	Het
Sim1	A	T	10: 50,784,057 (GRCm39)	I104F	probably damaging	Het
Slc1a3	T	C	15: 8,665,693 (GRCm39)	M519V	probably benign	Het
Smcp	T	A	3: 92,491,827 (GRCm39)	T7S	unknown	Het
Sp4	A	G	12: 118,264,551 (GRCm39)		probably benign	Het
Spag9	T	A	11: 93,984,365 (GRCm39)	I327N	probably damaging	Het
Thbs4	G	T	13: 92,890,918 (GRCm39)	H850N	probably benign	Het
Ubap2l	A	T	3: 89,928,680 (GRCm39)	S478T	possibly damaging	Het
Unc79	A	G	12: 103,054,693 (GRCm39)		probably benign	Het
Vmn2r85	A	G	10: 130,255,054 (GRCm39)		probably benign	Het
Wrap73	A	G	4: 154,226,957 (GRCm39)	D19G	possibly damaging	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,501,933 (GRCm39)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,487,169 (GRCm39)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,469,764 (GRCm39)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,445,013 (GRCm39)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,477,480 (GRCm39)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,474,712 (GRCm39)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,456,714 (GRCm39)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,502,723 (GRCm39)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,460,763 (GRCm39)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,482,887 (GRCm39)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,466,481 (GRCm39)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,504,669 (GRCm39)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,430,150 (GRCm39)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,456,676 (GRCm39)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,482,848 (GRCm39)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,491,944 (GRCm39)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,487,465 (GRCm39)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,480,782 (GRCm39)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,445,067 (GRCm39)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,486,857 (GRCm39)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,397,942 (GRCm39)	missense	probably benign
IGL03181:Hectd4	APN	5	121,492,021 (GRCm39)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,398,002 (GRCm39)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,466,445 (GRCm39)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
agamemnon	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
clymnestra	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
hector	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
helen	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,471,123 (GRCm39)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,392,242 (GRCm39)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,446,639 (GRCm39)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,400,651 (GRCm39)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,487,435 (GRCm39)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,467,668 (GRCm39)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,392,314 (GRCm39)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,397,927 (GRCm39)	missense	probably benign
R0410:Hectd4	UTSW	5	121,424,329 (GRCm39)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,481,145 (GRCm39)	splice site	probably null
R0442:Hectd4	UTSW	5	121,462,045 (GRCm39)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,502,653 (GRCm39)	splice site	probably null
R0469:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,469,770 (GRCm39)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,486,539 (GRCm39)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,442,400 (GRCm39)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,481,295 (GRCm39)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,486,688 (GRCm39)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,415,887 (GRCm39)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,424,526 (GRCm39)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,474,691 (GRCm39)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,445,096 (GRCm39)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,424,832 (GRCm39)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,424,799 (GRCm39)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,448,662 (GRCm39)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,488,548 (GRCm39)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,459,570 (GRCm39)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,456,687 (GRCm39)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,491,758 (GRCm39)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,466,576 (GRCm39)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,462,019 (GRCm39)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,487,322 (GRCm39)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,439,941 (GRCm39)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,455,308 (GRCm39)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,436,167 (GRCm39)	missense	probably benign
R1715:Hectd4	UTSW	5	121,482,881 (GRCm39)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,487,593 (GRCm39)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,496,366 (GRCm39)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,429,254 (GRCm39)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,435,243 (GRCm39)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,419,981 (GRCm39)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,493,692 (GRCm39)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,471,487 (GRCm39)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,456,702 (GRCm39)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,453,206 (GRCm39)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,491,600 (GRCm39)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,458,089 (GRCm39)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,402,030 (GRCm39)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,358,683 (GRCm39)	missense	unknown
R2904:Hectd4	UTSW	5	121,430,787 (GRCm39)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,397,936 (GRCm39)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,458,164 (GRCm39)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,441,588 (GRCm39)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,415,897 (GRCm39)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,358,567 (GRCm39)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,488,544 (GRCm39)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,424,320 (GRCm39)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,452,970 (GRCm39)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,488,494 (GRCm39)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,424,482 (GRCm39)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,435,266 (GRCm39)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,487,279 (GRCm39)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,487,118 (GRCm39)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,463,314 (GRCm39)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,441,678 (GRCm39)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,480,040 (GRCm39)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,486,505 (GRCm39)	missense	probably benign
R4781:Hectd4	UTSW	5	121,444,170 (GRCm39)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,460,735 (GRCm39)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,401,954 (GRCm39)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,497,378 (GRCm39)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,460,753 (GRCm39)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,467,628 (GRCm39)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,466,262 (GRCm39)	splice site	probably null
R5129:Hectd4	UTSW	5	121,481,573 (GRCm39)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,482,887 (GRCm39)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,481,739 (GRCm39)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,402,037 (GRCm39)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,442,511 (GRCm39)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,453,464 (GRCm39)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,448,666 (GRCm39)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,404,337 (GRCm39)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,445,011 (GRCm39)	missense	probably benign
R5507:Hectd4	UTSW	5	121,419,164 (GRCm39)	missense	unknown
R5552:Hectd4	UTSW	5	121,480,914 (GRCm39)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,486,878 (GRCm39)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,491,565 (GRCm39)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,486,682 (GRCm39)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,445,587 (GRCm39)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,481,288 (GRCm39)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,462,037 (GRCm39)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,446,941 (GRCm39)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,477,561 (GRCm39)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,392,283 (GRCm39)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,488,508 (GRCm39)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,488,655 (GRCm39)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,494,259 (GRCm39)	splice site	probably null
R6540:Hectd4	UTSW	5	121,441,634 (GRCm39)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,458,147 (GRCm39)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,415,788 (GRCm39)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,491,574 (GRCm39)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,502,631 (GRCm39)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,437,660 (GRCm39)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,411,692 (GRCm39)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,446,405 (GRCm39)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,467,136 (GRCm39)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,452,944 (GRCm39)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,419,995 (GRCm39)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,462,024 (GRCm39)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,496,196 (GRCm39)	splice site	probably null
R7482:Hectd4	UTSW	5	121,501,941 (GRCm39)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,435,172 (GRCm39)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,481,728 (GRCm39)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,453,573 (GRCm39)	splice site	probably null
R7560:Hectd4	UTSW	5	121,392,405 (GRCm39)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,429,288 (GRCm39)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,487,522 (GRCm39)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,456,798 (GRCm39)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,392,434 (GRCm39)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,462,094 (GRCm39)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,459,627 (GRCm39)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,358,680 (GRCm39)	missense	unknown
R7731:Hectd4	UTSW	5	121,445,077 (GRCm39)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,474,692 (GRCm39)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,443,784 (GRCm39)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,467,631 (GRCm39)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,469,880 (GRCm39)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,392,291 (GRCm39)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,448,692 (GRCm39)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,477,581 (GRCm39)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,459,461 (GRCm39)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,471,012 (GRCm39)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,424,439 (GRCm39)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,456,819 (GRCm39)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,477,607 (GRCm39)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,404,424 (GRCm39)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,455,288 (GRCm39)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,397,957 (GRCm39)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,446,421 (GRCm39)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,481,210 (GRCm39)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,467,172 (GRCm39)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,487,073 (GRCm39)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,442,489 (GRCm39)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,491,661 (GRCm39)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,448,714 (GRCm39)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,488,557 (GRCm39)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,501,838 (GRCm39)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,419,936 (GRCm39)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,461,994 (GRCm39)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,433,541 (GRCm39)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,466,305 (GRCm39)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,415,819 (GRCm39)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,496,347 (GRCm39)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,441,629 (GRCm39)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,392,422 (GRCm39)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,451,955 (GRCm39)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,411,677 (GRCm39)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,467,619 (GRCm39)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,496,238 (GRCm39)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,487,097 (GRCm39)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,391,967 (GRCm39)	missense
R9162:Hectd4	UTSW	5	121,445,042 (GRCm39)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,446,690 (GRCm39)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,437,551 (GRCm39)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,433,496 (GRCm39)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,487,028 (GRCm39)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,486,952 (GRCm39)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,437,708 (GRCm39)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,472,492 (GRCm39)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,460,864 (GRCm39)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,452,981 (GRCm39)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,502,616 (GRCm39)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,459,617 (GRCm39)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,472,532 (GRCm39)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,424,844 (GRCm39)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,392,254 (GRCm39)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,472,415 (GRCm39)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,487,700 (GRCm39)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,459,467 (GRCm39)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,433,566 (GRCm39)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,496,383 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AAGCAAGCCTGAGGACATGCTG -3'
(R):5'- TCTGTGAGACACACTGTGAACTGC -3'

Sequencing Primer
(F):5'- GACATGCTGTGGTTCCACC -3'
(R):5'- AGGCGCTGATGCTCATGG -3'

Posted On

2013-04-11