Mutagenetix > Incidental Mutation

Incidental Mutation 'R1928:Synj2'

215109

Institutional Source

Beutler Lab

Gene Symbol

Synj2

Ensembl Gene

ENSMUSG00000023805

Gene Name

synaptojanin 2

Synonyms

SJ2

MMRRC Submission

039946-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R1928 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

5991555-6094565 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 6040542 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 121 (I121N)

Ref Sequence

ENSEMBL: ENSMUSP00000111456 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061091] [ENSMUST00000080283] [ENSMUST00000115788] [ENSMUST00000115789] [ENSMUST00000115790] [ENSMUST00000115791] [ENSMUST00000134767] [ENSMUST00000146009] [ENSMUST00000142409]

AlphaFold

Q9D2G5

Predicted Effect

probably damaging
Transcript: ENSMUST00000061091
AA Change: I121N

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000060382
Gene: ENSMUSG00000023805
AA Change: I121N

Domain	Start	End	E-Value	Type
Pfam:Syja_N	1	263	2.5e-72	PFAM
Blast:IPPc	394	423	3e-6	BLAST
IPPc	443	785	3.72e-128	SMART
DUF1866	778	923	1.04e-73	SMART
low complexity region	926	940	N/A	INTRINSIC
low complexity region	965	976	N/A	INTRINSIC
low complexity region	1012	1027	N/A	INTRINSIC
low complexity region	1127	1139	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000080283
AA Change: I206N

PolyPhen 2 Score 0.908 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000079164
Gene: ENSMUSG00000023805
AA Change: I206N

Domain	Start	End	E-Value	Type
Pfam:Syja_N	60	348	5.5e-78	PFAM
Blast:IPPc	479	508	3e-6	BLAST
IPPc	528	870	3.72e-128	SMART
DUF1866	863	1008	1.04e-73	SMART
low complexity region	1011	1025	N/A	INTRINSIC
low complexity region	1050	1061	N/A	INTRINSIC
low complexity region	1167	1179	N/A	INTRINSIC
low complexity region	1217	1234	N/A	INTRINSIC
low complexity region	1263	1277	N/A	INTRINSIC
low complexity region	1293	1306	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000115788

SMART Domains

Protein: ENSMUSP00000111454
Gene: ENSMUSG00000023805

Domain	Start	End	E-Value	Type
Pfam:Syja_N	1	108	4.8e-28	PFAM
Blast:IPPc	239	268	2e-6	BLAST
IPPc	288	630	3.72e-128	SMART
DUF1866	623	768	1.04e-73	SMART
low complexity region	771	785	N/A	INTRINSIC
low complexity region	810	821	N/A	INTRINSIC
low complexity region	927	939	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000115789

SMART Domains

Protein: ENSMUSP00000111455
Gene: ENSMUSG00000023805

Domain	Start	End	E-Value	Type
Pfam:Syja_N	1	187	2.3e-60	PFAM
Blast:IPPc	318	347	2e-6	BLAST
IPPc	367	709	3.72e-128	SMART
DUF1866	702	847	1.04e-73	SMART
low complexity region	850	864	N/A	INTRINSIC
low complexity region	889	900	N/A	INTRINSIC
low complexity region	1006	1018	N/A	INTRINSIC
low complexity region	1056	1073	N/A	INTRINSIC
low complexity region	1102	1116	N/A	INTRINSIC
low complexity region	1132	1145	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115790
AA Change: I121N

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000111456
Gene: ENSMUSG00000023805
AA Change: I121N

Domain	Start	End	E-Value	Type
Pfam:Syja_N	1	263	3e-72	PFAM
Blast:IPPc	394	423	3e-6	BLAST
IPPc	443	785	3.72e-128	SMART
DUF1866	778	923	1.04e-73	SMART
low complexity region	926	940	N/A	INTRINSIC
low complexity region	965	976	N/A	INTRINSIC
low complexity region	1012	1027	N/A	INTRINSIC
low complexity region	1127	1139	N/A	INTRINSIC
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1223	1237	N/A	INTRINSIC
low complexity region	1253	1266	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000115791
AA Change: I206N

PolyPhen 2 Score 0.908 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000111457
Gene: ENSMUSG00000023805
AA Change: I206N

Domain	Start	End	E-Value	Type
Pfam:Syja_N	61	343	8.5e-67	PFAM
Blast:IPPc	479	508	3e-6	BLAST
IPPc	528	870	3.72e-128	SMART
DUF1866	863	1008	1.04e-73	SMART
low complexity region	1011	1025	N/A	INTRINSIC
low complexity region	1050	1061	N/A	INTRINSIC
low complexity region	1097	1112	N/A	INTRINSIC
low complexity region	1212	1224	N/A	INTRINSIC
low complexity region	1262	1279	N/A	INTRINSIC
low complexity region	1308	1322	N/A	INTRINSIC
low complexity region	1338	1351	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000134767

Predicted Effect

probably damaging
Transcript: ENSMUST00000146009
AA Change: I206N

PolyPhen 2 Score 0.963 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000122381
Gene: ENSMUSG00000023805
AA Change: I206N

Domain	Start	End	E-Value	Type
Pfam:Syja_N	60	348	3.6e-78	PFAM
Blast:IPPc	479	508	3e-6	BLAST
IPPc	528	870	3.72e-128	SMART
DUF1866	863	1008	1.04e-73	SMART
low complexity region	1011	1025	N/A	INTRINSIC
low complexity region	1050	1061	N/A	INTRINSIC
low complexity region	1097	1112	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000142409

SMART Domains

Protein: ENSMUSP00000120006
Gene: ENSMUSG00000023805

Domain	Start	End	E-Value	Type
Pfam:Syja_N	1	108	2.5e-28	PFAM
Blast:IPPc	239	268	2e-6	BLAST
IPPc	288	630	3.72e-128	SMART

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.6%

Validation Efficiency

98% (120/123)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
PHENOTYPE: Homozygotes for an ENU-induced allele show progressive hearing loss and cochlear hair cell degeneration associated with fusion of stereocilia followed by total loss of hair bundles and cochlear ganglion degeneration. No vestibular dysfunction or other behavioral deficits are observed. [provided by MGI curators]

Allele List at MGI

All alleles(5) : Targeted, other(1) Gene trapped(4)

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630073D07Rik	T	G	6: 132,603,564 (GRCm39)	Q64P	unknown	Het
Adgrv1	A	T	13: 81,668,905 (GRCm39)	F1830L	probably benign	Het
Anks3	C	T	16: 4,763,918 (GRCm39)		probably null	Het
Aqp12	G	A	1: 92,934,332 (GRCm39)	D70N	probably damaging	Het
B4galnt4	C	T	7: 140,648,061 (GRCm39)	R526*	probably null	Het
Best2	A	G	8: 85,737,882 (GRCm39)	F171S	probably benign	Het
C2cd5	A	T	6: 142,958,956 (GRCm39)	V965D	probably damaging	Het
Cd33	T	C	7: 43,179,303 (GRCm39)	E282G	probably benign	Het
Cep95	C	T	11: 106,681,554 (GRCm39)		probably benign	Het
Chd6	A	T	2: 160,809,920 (GRCm39)		probably benign	Het
Clec4d	A	T	6: 123,244,120 (GRCm39)		probably null	Het
Cntd1	C	T	11: 101,174,678 (GRCm39)	S128L	probably damaging	Het
Col19a1	T	C	1: 24,490,835 (GRCm39)		probably benign	Het
Ctrb1	C	A	8: 112,415,324 (GRCm39)	V117L	probably benign	Het
Dclk1	T	C	3: 55,154,942 (GRCm39)	V124A	possibly damaging	Het
Dcp2	T	C	18: 44,538,638 (GRCm39)		probably null	Het
Dctn1	C	A	6: 83,176,166 (GRCm39)		probably benign	Het
Dennd11	A	G	6: 40,388,648 (GRCm39)	F238L	probably benign	Het
Ephb3	T	A	16: 21,041,045 (GRCm39)	M701K	possibly damaging	Het
Ero1b	A	G	13: 12,616,648 (GRCm39)	E359G	probably damaging	Het
Exosc8	G	T	3: 54,636,266 (GRCm39)	A255E	probably damaging	Het
Fhip1a	G	A	3: 85,595,838 (GRCm39)	P349L	probably damaging	Het
Fndc3c1	C	T	X: 105,477,128 (GRCm39)	A824T	probably benign	Het
Gja10	G	A	4: 32,601,812 (GRCm39)	Q191*	probably null	Het
Gm1587	C	T	14: 78,036,288 (GRCm39)	R6Q	unknown	Het
Gm3409	A	G	5: 146,476,418 (GRCm39)	I190V	probably benign	Het
Gm4952	G	T	19: 12,600,973 (GRCm39)	M64I	probably damaging	Het
Gramd1b	C	T	9: 40,217,765 (GRCm39)	M595I	possibly damaging	Het
Grik1	C	T	16: 87,848,241 (GRCm39)	V176M	probably damaging	Het
Grin2b	A	G	6: 136,021,044 (GRCm39)	C86R	probably damaging	Het
Gtse1	C	T	15: 85,746,264 (GRCm39)		probably benign	Het
Hectd2	T	C	19: 36,589,719 (GRCm39)	Y615H	probably damaging	Het
Hydin	T	C	8: 111,229,579 (GRCm39)	F1552S	possibly damaging	Het
Ifi207	A	G	1: 173,557,211 (GRCm39)	V516A	possibly damaging	Het
Igfbp5	G	T	1: 72,913,184 (GRCm39)	P39T	probably damaging	Het
Il23r	T	A	6: 67,400,719 (GRCm39)	D537V	possibly damaging	Het
Invs	A	G	4: 48,390,095 (GRCm39)	Y251C	probably damaging	Het
Isl1	G	T	13: 116,444,953 (GRCm39)	H25Q	probably damaging	Het
Kif13a	A	G	13: 46,966,221 (GRCm39)	L399P	probably damaging	Het
Klhl1	A	G	14: 96,584,225 (GRCm39)	V335A	probably benign	Het
Klhl14	C	A	18: 21,784,843 (GRCm39)	A195S	probably damaging	Het
Lepr	A	G	4: 101,639,927 (GRCm39)		probably benign	Het
Map1b	A	C	13: 99,567,454 (GRCm39)	S1756A	unknown	Het
Med23	C	T	10: 24,785,710 (GRCm39)	A877V	probably benign	Het
Mfsd4b2	T	C	10: 39,797,458 (GRCm39)	Y299C	probably damaging	Het
Mipol1	T	A	12: 57,379,205 (GRCm39)	M221K	probably damaging	Het
Muc4	A	T	16: 32,569,350 (GRCm39)	S137C	probably damaging	Het
Mutyh	T	C	4: 116,673,855 (GRCm39)	Y189H	probably damaging	Het
Nabp2	T	C	10: 128,245,182 (GRCm39)	T21A	possibly damaging	Het
Nalf1	T	C	8: 9,820,217 (GRCm39)	T268A	probably benign	Het
Nell1	T	A	7: 50,350,943 (GRCm39)	V530E	possibly damaging	Het
Npc1	G	C	18: 12,346,435 (GRCm39)	P254A	possibly damaging	Het
Nup153	A	G	13: 46,854,502 (GRCm39)	V530A	probably damaging	Het
Nxpe2	T	C	9: 48,237,914 (GRCm39)	T114A	probably damaging	Het
Or10j5	T	C	1: 172,784,881 (GRCm39)	V173A	probably damaging	Het
Or11h4b	A	G	14: 50,918,872 (GRCm39)	V73A	probably benign	Het
Or7e177	T	C	9: 20,212,354 (GRCm39)	V287A	probably benign	Het
Pabpc1l	T	A	2: 163,874,174 (GRCm39)	I193N	possibly damaging	Het
Pabpc4l	A	G	3: 46,401,066 (GRCm39)	Y193H	probably damaging	Het
Pank1	T	C	19: 34,856,281 (GRCm39)	S66G	probably benign	Het
Pcdhb1	A	T	18: 37,399,233 (GRCm39)	K395*	probably null	Het
Pcna	A	G	2: 132,093,817 (GRCm39)		probably benign	Het
Pgr	T	A	9: 8,903,630 (GRCm39)	Y550*	probably null	Het
Phf11a	A	G	14: 59,519,316 (GRCm39)		probably benign	Het
Pkhd1	A	T	1: 20,151,524 (GRCm39)		probably benign	Het
Pofut2	T	A	10: 77,096,642 (GRCm39)	Y122*	probably null	Het
Pole	A	G	5: 110,475,644 (GRCm39)	M1818V	probably benign	Het
Rapgef3	A	G	15: 97,647,914 (GRCm39)	L696P	probably damaging	Het
Rasal3	T	C	17: 32,616,327 (GRCm39)	D310G	probably damaging	Het
Rhoq	A	G	17: 87,302,486 (GRCm39)	K141E	probably benign	Het
Rilpl1	A	G	5: 124,652,813 (GRCm39)		probably benign	Het
Rnaseh1	T	C	12: 28,703,088 (GRCm39)	S91P	probably benign	Het
Rsf1	G	GACGGCGGCT	7: 97,229,116 (GRCm39)		probably benign	Het
Saa1	C	A	7: 46,391,856 (GRCm39)	G31W	probably null	Het
Saxo5	T	A	8: 3,536,947 (GRCm39)	I431K	possibly damaging	Het
Scaf8	A	G	17: 3,218,352 (GRCm39)	T241A	unknown	Het
Scarb2	C	T	5: 92,592,125 (GRCm39)	A473T	possibly damaging	Het
Scnn1b	T	C	7: 121,509,670 (GRCm39)	F273S	probably damaging	Het
Sdccag8	T	C	1: 176,656,536 (GRCm39)	V136A	probably damaging	Het
Sdr16c6	A	T	4: 4,069,926 (GRCm39)	V138E	probably damaging	Het
Sf3a3	G	A	4: 124,615,886 (GRCm39)	A180T	possibly damaging	Het
Slc16a5	T	A	11: 115,360,842 (GRCm39)	S342T	probably damaging	Het
Slc18a1	A	G	8: 69,526,464 (GRCm39)	S75P	probably benign	Het
Slc7a6	T	A	8: 106,920,120 (GRCm39)		probably benign	Het
Smco3	G	A	6: 136,808,845 (GRCm39)	Q10*	probably null	Het
Spem2	T	C	11: 69,708,290 (GRCm39)	Q225R	probably benign	Het
Ssb	G	T	2: 69,697,901 (GRCm39)		probably null	Het
Stx8	T	C	11: 68,000,106 (GRCm39)	I182T	probably damaging	Het
Tbc1d1	T	C	5: 64,502,643 (GRCm39)	Y1055H	probably damaging	Het
Thbs3	G	T	3: 89,125,067 (GRCm39)	R52L	probably damaging	Het
Tmem101	C	T	11: 102,044,222 (GRCm39)	V222I	probably benign	Het
Tnks2	C	T	19: 36,823,068 (GRCm39)	Q112*	probably null	Het
Tnrc6b	T	A	15: 80,764,924 (GRCm39)	W809R	probably damaging	Het
Trib2	T	A	12: 15,865,454 (GRCm39)	R16S	probably damaging	Het
Trim12a	T	C	7: 103,956,331 (GRCm39)	N70D	probably damaging	Het
Trim55	T	A	3: 19,716,046 (GRCm39)		probably null	Het
Tspyl5	T	A	15: 33,687,153 (GRCm39)	D264V	probably damaging	Het
Ttn	A	G	2: 76,555,388 (GRCm39)	V30539A	probably damaging	Het
Tubgcp3	A	G	8: 12,713,988 (GRCm39)	F43S	possibly damaging	Het
Tut7	A	G	13: 59,964,548 (GRCm39)	L209P	probably damaging	Het
Ube3d	A	G	9: 86,305,056 (GRCm39)	L262P	probably damaging	Het
Ugt1a7c	T	C	1: 88,023,651 (GRCm39)	V270A	probably benign	Het
Vav3	T	A	3: 109,413,738 (GRCm39)	F225L	possibly damaging	Het
Vmn1r21	G	T	6: 57,821,077 (GRCm39)	Y122*	probably null	Het
Vmn1r225	C	A	17: 20,723,071 (GRCm39)	Q171K	probably benign	Het
Vmn2r103	T	C	17: 20,032,029 (GRCm39)	V601A	possibly damaging	Het
Vmn2r19	T	A	6: 123,308,589 (GRCm39)	N555K	probably damaging	Het
Vps33a	G	A	5: 123,696,684 (GRCm39)	A323V	probably benign	Het
Wdr77	C	T	3: 105,874,618 (GRCm39)	P337S	probably benign	Het
Wnk1	A	T	6: 119,929,884 (GRCm39)	I93N	probably damaging	Het
Zbtb9	T	A	17: 27,193,612 (GRCm39)	I339N	probably damaging	Het
Zfp202	T	A	9: 40,121,083 (GRCm39)	D241E	probably damaging	Het
Zfp235	T	A	7: 23,840,563 (GRCm39)	Y327*	probably null	Het
Zfp820	A	C	17: 22,038,316 (GRCm39)	D337E	probably benign	Het
Zfp944	T	C	17: 22,560,065 (GRCm39)	T14A	probably damaging	Het
Zfy1	A	G	Y: 729,733 (GRCm39)	V303A	unknown	Het
Zfyve26	C	T	12: 79,286,744 (GRCm39)	W2281*	probably null	Het

Other mutations in Synj2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Synj2	APN	17	6,088,201 (GRCm39)	missense	possibly damaging	0.48
IGL01399:Synj2	APN	17	6,060,046 (GRCm39)	missense	probably damaging	1.00
IGL01793:Synj2	APN	17	6,088,321 (GRCm39)	missense	probably benign	0.01
IGL01793:Synj2	APN	17	6,077,500 (GRCm39)	nonsense	probably null
IGL02096:Synj2	APN	17	6,040,628 (GRCm39)	missense	probably damaging	1.00
IGL02115:Synj2	APN	17	6,067,865 (GRCm39)	missense	probably damaging	1.00
IGL02222:Synj2	APN	17	6,087,755 (GRCm39)	missense	probably benign	0.04
IGL02478:Synj2	APN	17	6,088,199 (GRCm39)	missense	probably benign	0.00
IGL02634:Synj2	APN	17	6,080,035 (GRCm39)	missense	probably damaging	1.00
IGL02652:Synj2	APN	17	6,067,868 (GRCm39)	missense	probably damaging	1.00
IGL02681:Synj2	APN	17	6,040,611 (GRCm39)	missense	probably damaging	1.00
IGL02719:Synj2	APN	17	6,047,192 (GRCm39)	missense	probably benign	0.02
IGL03253:Synj2	APN	17	6,053,434 (GRCm39)	splice site	probably null
IGL03365:Synj2	APN	17	6,069,679 (GRCm39)	missense	probably damaging	1.00
I2288:Synj2	UTSW	17	6,072,542 (GRCm39)	splice site	probably benign
I2289:Synj2	UTSW	17	6,072,542 (GRCm39)	splice site	probably benign
R0389:Synj2	UTSW	17	6,080,058 (GRCm39)	missense	probably benign	0.35
R0433:Synj2	UTSW	17	6,084,123 (GRCm39)	missense	probably damaging	1.00
R0530:Synj2	UTSW	17	6,058,380 (GRCm39)	missense	possibly damaging	0.88
R0539:Synj2	UTSW	17	6,047,163 (GRCm39)	start codon destroyed	probably null	0.63
R0556:Synj2	UTSW	17	6,088,230 (GRCm39)	nonsense	probably null
R1263:Synj2	UTSW	17	6,069,634 (GRCm39)	missense	probably damaging	0.99
R1443:Synj2	UTSW	17	6,073,940 (GRCm39)	missense	probably damaging	0.99
R1450:Synj2	UTSW	17	6,077,599 (GRCm39)	splice site	probably benign
R1532:Synj2	UTSW	17	6,084,194 (GRCm39)	missense	probably benign	0.00
R1542:Synj2	UTSW	17	6,075,292 (GRCm39)	missense	probably benign	0.01
R1809:Synj2	UTSW	17	6,076,826 (GRCm39)	missense	possibly damaging	0.95
R1875:Synj2	UTSW	17	6,078,825 (GRCm39)	missense	possibly damaging	0.69
R1897:Synj2	UTSW	17	6,072,412 (GRCm39)	nonsense	probably null
R2008:Synj2	UTSW	17	6,047,221 (GRCm39)	missense	probably damaging	1.00
R2060:Synj2	UTSW	17	6,087,755 (GRCm39)	missense	probably benign	0.04
R2109:Synj2	UTSW	17	6,063,966 (GRCm39)	missense	probably benign	0.00
R2332:Synj2	UTSW	17	6,074,069 (GRCm39)	missense	probably damaging	0.99
R2413:Synj2	UTSW	17	6,078,849 (GRCm39)	missense	probably damaging	1.00
R3684:Synj2	UTSW	17	6,078,718 (GRCm39)	missense	probably damaging	0.97
R4111:Synj2	UTSW	17	6,058,240 (GRCm39)	missense	probably benign	0.02
R4113:Synj2	UTSW	17	6,058,240 (GRCm39)	missense	probably benign	0.02
R4654:Synj2	UTSW	17	6,063,813 (GRCm39)	missense	probably damaging	1.00
R4797:Synj2	UTSW	17	6,084,163 (GRCm39)	missense	probably damaging	1.00
R4812:Synj2	UTSW	17	6,060,939 (GRCm39)	missense	probably damaging	1.00
R4873:Synj2	UTSW	17	6,038,343 (GRCm39)	intron	probably benign
R4875:Synj2	UTSW	17	6,038,343 (GRCm39)	intron	probably benign
R5110:Synj2	UTSW	17	6,087,990 (GRCm39)	missense	probably benign	0.06
R5205:Synj2	UTSW	17	5,991,793 (GRCm39)	missense	probably damaging	1.00
R5504:Synj2	UTSW	17	6,086,750 (GRCm39)	missense	possibly damaging	0.85
R5593:Synj2	UTSW	17	6,088,390 (GRCm39)	makesense	probably null
R5690:Synj2	UTSW	17	6,085,802 (GRCm39)	missense	probably benign	0.00
R5870:Synj2	UTSW	17	6,088,128 (GRCm39)	missense	probably benign	0.00
R6084:Synj2	UTSW	17	6,088,373 (GRCm39)	missense	probably damaging	1.00
R6084:Synj2	UTSW	17	6,067,889 (GRCm39)	missense	probably damaging	0.98
R6158:Synj2	UTSW	17	6,036,487 (GRCm39)	missense	probably benign	0.00
R6159:Synj2	UTSW	17	6,036,327 (GRCm39)	missense	probably damaging	1.00
R6160:Synj2	UTSW	17	6,058,336 (GRCm39)	missense	possibly damaging	0.92
R6278:Synj2	UTSW	17	6,026,149 (GRCm39)	missense	probably damaging	1.00
R6406:Synj2	UTSW	17	6,069,846 (GRCm39)	intron	probably benign
R6531:Synj2	UTSW	17	6,084,114 (GRCm39)	missense	probably damaging	1.00
R6729:Synj2	UTSW	17	6,036,289 (GRCm39)	start codon destroyed	probably null	1.00
R6774:Synj2	UTSW	17	6,088,290 (GRCm39)	missense	possibly damaging	0.87
R6792:Synj2	UTSW	17	6,040,565 (GRCm39)	missense	probably benign	0.01
R6844:Synj2	UTSW	17	6,026,081 (GRCm39)	missense	probably damaging	0.96
R6865:Synj2	UTSW	17	6,067,844 (GRCm39)	nonsense	probably null
R7178:Synj2	UTSW	17	6,076,754 (GRCm39)	missense	possibly damaging	0.95
R7286:Synj2	UTSW	17	6,088,220 (GRCm39)	missense	possibly damaging	0.79
R7403:Synj2	UTSW	17	6,088,005 (GRCm39)	missense	possibly damaging	0.76
R7451:Synj2	UTSW	17	6,080,066 (GRCm39)	missense	possibly damaging	0.68
R7501:Synj2	UTSW	17	6,040,514 (GRCm39)	missense	possibly damaging	0.79
R7730:Synj2	UTSW	17	6,066,562 (GRCm39)	missense	probably benign	0.33
R7799:Synj2	UTSW	17	6,088,098 (GRCm39)	missense	probably benign	0.10
R7804:Synj2	UTSW	17	6,069,809 (GRCm39)	missense	unknown
R7841:Synj2	UTSW	17	6,094,419 (GRCm39)	missense	unknown
R8347:Synj2	UTSW	17	6,060,060 (GRCm39)	missense	probably damaging	1.00
R8358:Synj2	UTSW	17	6,074,080 (GRCm39)	nonsense	probably null
R8391:Synj2	UTSW	17	5,991,796 (GRCm39)	missense	probably damaging	0.99
R8725:Synj2	UTSW	17	6,088,015 (GRCm39)	missense	possibly damaging	0.48
R8787:Synj2	UTSW	17	6,036,514 (GRCm39)	missense	possibly damaging	0.57
R8877:Synj2	UTSW	17	6,087,941 (GRCm39)	missense	probably damaging	1.00
R9091:Synj2	UTSW	17	6,067,875 (GRCm39)	missense	possibly damaging	0.88
R9121:Synj2	UTSW	17	6,040,599 (GRCm39)	missense	probably damaging	1.00
R9147:Synj2	UTSW	17	6,084,172 (GRCm39)	missense	probably damaging	1.00
R9148:Synj2	UTSW	17	6,084,172 (GRCm39)	missense	probably damaging	1.00
R9270:Synj2	UTSW	17	6,067,875 (GRCm39)	missense	possibly damaging	0.88
R9489:Synj2	UTSW	17	6,063,794 (GRCm39)	missense	probably benign	0.00
R9605:Synj2	UTSW	17	6,063,794 (GRCm39)	missense	probably benign	0.00
R9720:Synj2	UTSW	17	6,040,584 (GRCm39)	missense	probably benign
R9773:Synj2	UTSW	17	6,094,232 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAGCATTTGTAGAGCCTGGTAG -3'
(R):5'- ATTGGACACGCAGACTTTGTG -3'

Sequencing Primer
(F):5'- TATTGCATGTGCCTCTGC -3'
(R):5'- AGACTTTGTGTGCCCCGGTAC -3'

Posted On

2014-07-14