Incidental Mutation 'R1930:Msln'
ID 215311
Institutional Source Beutler Lab
Gene Symbol Msln
Ensembl Gene ENSMUSG00000063011
Gene Name mesothelin
Synonyms megakaryocyte potentiating factor, MPF
MMRRC Submission 039948-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.061) question?
Stock # R1930 (G1)
Quality Score 225
Status Not validated
Chromosome 17
Chromosomal Location 25967587-25973352 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 25970896 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Lysine at position 150 (N150K)
Ref Sequence ENSEMBL: ENSMUSP00000075279 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]
AlphaFold Q61468
Predicted Effect probably benign
Transcript: ENSMUST00000047098
SMART Domains Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Pfam:Mesothelin 29 589 2.8e-70 PFAM
low complexity region 633 653 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000075884
AA Change: N150K

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011
AA Change: N150K

DomainStartEndE-ValueType
Pfam:Mesothelin 1 624 N/A PFAM
Coding Region Coverage
  • 1x: 97.5%
  • 3x: 97.0%
  • 10x: 95.6%
  • 20x: 93.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for disruptions in this allele display a normal phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 72 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2410002F23Rik T A 7: 43,900,540 (GRCm39) D148E probably damaging Het
4930590J08Rik T C 6: 91,892,002 (GRCm39) V88A probably benign Het
4933430I17Rik A T 4: 62,450,519 (GRCm39) D31V possibly damaging Het
Acsl1 C A 8: 46,984,023 (GRCm39) A514E probably benign Het
Adam29 T C 8: 56,326,124 (GRCm39) Y110C probably damaging Het
Adamts20 T A 15: 94,301,891 (GRCm39) H27L probably benign Het
Ago2 A T 15: 72,991,204 (GRCm39) I578N probably damaging Het
Arsi G A 18: 61,049,723 (GRCm39) G202E probably benign Het
AW011738 T C 4: 156,287,997 (GRCm39) probably benign Het
Bid C T 6: 120,874,216 (GRCm39) A110T possibly damaging Het
Cav1 A T 6: 17,339,331 (GRCm39) I139F probably damaging Het
Cep152 A T 2: 125,460,291 (GRCm39) probably null Het
Chrnb1 A G 11: 69,683,563 (GRCm39) L261P possibly damaging Het
Cntn3 C T 6: 102,219,014 (GRCm39) W535* probably null Het
Col4a4 A C 1: 82,444,321 (GRCm39) probably null Het
Daam2 A T 17: 49,769,241 (GRCm39) probably null Het
Ddx23 G A 15: 98,548,599 (GRCm39) R370W possibly damaging Het
Dgkh T C 14: 78,853,945 (GRCm39) I265V probably damaging Het
Ece1 A G 4: 137,666,074 (GRCm39) K306R probably benign Het
Fat1 C T 8: 45,497,265 (GRCm39) T4250M possibly damaging Het
Fezf1 T G 6: 23,246,906 (GRCm39) I309L probably damaging Het
Gabra4 T C 5: 71,795,580 (GRCm39) K206E probably damaging Het
Glt8d2 G T 10: 82,500,476 (GRCm39) S91R probably benign Het
H2-Oa A T 17: 34,312,873 (GRCm39) H50L possibly damaging Het
Hsd17b2 T C 8: 118,485,643 (GRCm39) Y369H possibly damaging Het
Hspg2 T A 4: 137,267,541 (GRCm39) S2050T probably damaging Het
Ice1 A T 13: 70,753,202 (GRCm39) S961R probably benign Het
Ift20 G A 11: 78,430,830 (GRCm39) V58I possibly damaging Het
Ippk T A 13: 49,603,494 (GRCm39) F367I probably damaging Het
Klkb1 T A 8: 45,728,514 (GRCm39) Q415L probably benign Het
Krtcap2 T A 3: 89,154,383 (GRCm39) N35K probably damaging Het
Lgi3 T C 14: 70,773,708 (GRCm39) V294A probably damaging Het
Lrp5 C T 19: 3,660,131 (GRCm39) V978I probably benign Het
Magi3 A T 3: 103,996,920 (GRCm39) D208E probably damaging Het
Mc2r T A 18: 68,540,853 (GRCm39) T147S probably benign Het
Mfsd4b1 C A 10: 39,882,070 (GRCm39) A72S probably benign Het
Naaa A G 5: 92,425,894 (GRCm39) V33A probably benign Het
Nol10 A G 12: 17,398,555 (GRCm39) M1V probably null Het
Or4c3 G A 2: 89,851,505 (GRCm39) R302C probably benign Het
Or6c208 T A 10: 129,223,745 (GRCm39) M81K probably benign Het
Osbpl1a T A 18: 13,038,251 (GRCm39) Q269L probably benign Het
Pcnx2 C A 8: 126,614,453 (GRCm39) V333L probably benign Het
Peg10 A G 6: 4,755,778 (GRCm39) Y118C probably damaging Het
Pkhd1l1 A G 15: 44,366,733 (GRCm39) D737G possibly damaging Het
Ptgfr T A 3: 151,540,831 (GRCm39) T226S probably benign Het
Ptprz1 T A 6: 23,007,354 (GRCm39) V1639E probably damaging Het
Pttg1ip2 C A 5: 5,502,019 (GRCm39) W144C probably benign Het
Scgb2b20 T C 7: 33,065,621 (GRCm39) probably null Het
Sdr16c6 A G 4: 4,058,809 (GRCm39) V259A probably benign Het
Slc16a5 A T 11: 115,360,194 (GRCm39) I126F probably damaging Het
Slc51b T A 9: 65,322,478 (GRCm39) E21V probably damaging Het
Slc8a3 G T 12: 81,361,220 (GRCm39) T533N probably damaging Het
Snrnp40 C G 4: 130,271,836 (GRCm39) probably null Het
Spata3 A C 1: 85,949,783 (GRCm39) probably benign Het
Specc1l A G 10: 75,145,658 (GRCm39) D1101G probably damaging Het
Srf C A 17: 46,860,912 (GRCm39) G401C probably damaging Het
Stard9 C A 2: 120,504,117 (GRCm39) S221R probably damaging Het
Stradb A C 1: 59,030,264 (GRCm39) N173H probably benign Het
Sugp1 T A 8: 70,524,190 (GRCm39) D598E probably benign Het
Tdg T A 10: 82,477,378 (GRCm39) L35Q probably damaging Het
Tekt2 T C 4: 126,216,610 (GRCm39) probably null Het
Tex10 C T 4: 48,456,800 (GRCm39) R637Q probably benign Het
Tmprss2 T C 16: 97,370,262 (GRCm39) S301G probably benign Het
Tnrc18 A T 5: 142,762,079 (GRCm39) N515K unknown Het
Togaram1 A G 12: 65,013,709 (GRCm39) Y320C probably damaging Het
Tspyl3 A G 2: 153,066,717 (GRCm39) F174L probably damaging Het
Ttn C T 2: 76,553,193 (GRCm39) D31099N probably damaging Het
Ugt2b1 T A 5: 87,065,700 (GRCm39) L446F probably damaging Het
Utp25 T A 1: 192,800,617 (GRCm39) K401I probably damaging Het
Wdfy3 A G 5: 102,089,358 (GRCm39) L612P probably damaging Het
Zfp109 G A 7: 23,928,161 (GRCm39) T424M probably damaging Het
Zfp60 T A 7: 27,436,382 (GRCm39) M1K probably null Het
Other mutations in Msln
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01739:Msln APN 17 25,969,004 (GRCm39) critical splice donor site probably null
IGL02986:Msln APN 17 25,971,907 (GRCm39) splice site probably benign
R0349:Msln UTSW 17 25,969,250 (GRCm39) missense possibly damaging 0.69
R0562:Msln UTSW 17 25,971,980 (GRCm39) missense probably benign 0.16
R0845:Msln UTSW 17 25,969,770 (GRCm39) missense probably damaging 1.00
R1256:Msln UTSW 17 25,973,157 (GRCm39) missense probably damaging 1.00
R1305:Msln UTSW 17 25,972,001 (GRCm39) missense probably benign 0.00
R1651:Msln UTSW 17 25,972,382 (GRCm39) missense probably benign 0.00
R1996:Msln UTSW 17 25,973,193 (GRCm39) start codon destroyed possibly damaging 0.94
R4532:Msln UTSW 17 25,969,698 (GRCm39) missense probably damaging 0.98
R5004:Msln UTSW 17 25,973,193 (GRCm39) start codon destroyed possibly damaging 0.94
R5157:Msln UTSW 17 25,971,957 (GRCm39) missense probably benign 0.01
R5159:Msln UTSW 17 25,970,563 (GRCm39) missense probably benign 0.01
R5510:Msln UTSW 17 25,968,847 (GRCm39) missense probably benign 0.15
R6385:Msln UTSW 17 25,970,115 (GRCm39) missense probably benign 0.19
R6650:Msln UTSW 17 25,969,144 (GRCm39) missense probably benign 0.00
R6682:Msln UTSW 17 25,971,993 (GRCm39) missense probably damaging 0.99
R7091:Msln UTSW 17 25,969,054 (GRCm39) missense probably damaging 1.00
R7472:Msln UTSW 17 25,969,708 (GRCm39) missense possibly damaging 0.95
R8085:Msln UTSW 17 25,971,942 (GRCm39) nonsense probably null
R8289:Msln UTSW 17 25,967,880 (GRCm39) missense possibly damaging 0.50
R9137:Msln UTSW 17 25,969,084 (GRCm39) missense probably benign 0.24
R9217:Msln UTSW 17 25,970,125 (GRCm39) missense probably benign 0.02
R9309:Msln UTSW 17 25,970,148 (GRCm39) missense possibly damaging 0.68
R9311:Msln UTSW 17 25,971,990 (GRCm39) missense probably benign 0.09
R9441:Msln UTSW 17 25,969,731 (GRCm39) missense probably benign 0.02
R9652:Msln UTSW 17 25,968,042 (GRCm39) missense probably damaging 1.00
R9723:Msln UTSW 17 25,969,008 (GRCm39) missense possibly damaging 0.55
R9798:Msln UTSW 17 25,972,771 (GRCm39) missense probably benign 0.01
X0002:Msln UTSW 17 25,971,284 (GRCm39) splice site probably null
Z1176:Msln UTSW 17 25,972,768 (GRCm39) missense possibly damaging 0.74
Predicted Primers PCR Primer
(F):5'- TGAAATCCATACACGCCCTG -3'
(R):5'- CAATGTGCAGTGACTGGGTG -3'

Sequencing Primer
(F):5'- TGTGCACACAGCCAGCATC -3'
(R):5'- CAGGGAGGTACTTAGTGGAGTATC -3'
Posted On 2014-07-14