Mutagenetix > Incidental Mutation

Incidental Mutation 'R1931:Zfp735'

215382

Institutional Source

Beutler Lab

Gene Symbol

Zfp735

Ensembl Gene

ENSMUSG00000060630

Gene Name

zinc finger protein 735

Synonyms

1700012C15Rik

MMRRC Submission

039949-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R1931 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73688778-73713798 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73711851 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 540 (K540N)

Ref Sequence

ENSEMBL: ENSMUSP00000079269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080407]

AlphaFold

B1ARH2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000080407
AA Change: K540N

PolyPhen 2 Score 0.695 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000079269
Gene: ENSMUSG00000060630
AA Change: K540N

Domain	Start	End	E-Value	Type
KRAB	8	68	2.2e-34	SMART
ZnF_C2H2	483	505	4.38e1	SMART
ZnF_C2H2	511	533	2.67e-1	SMART
ZnF_C2H2	539	561	1.81e1	SMART
ZnF_C2H2	567	589	1.5e-4	SMART
ZnF_C2H2	595	617	4.87e-4	SMART
ZnF_C2H2	623	645	4.24e-4	SMART
ZnF_C2H2	651	673	2.27e-4	SMART
ZnF_C2H2	679	701	7.49e-5	SMART
ZnF_C2H2	707	729	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145996

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149560

Coding Region Coverage

1x: 97.3%
3x: 96.8%
10x: 95.3%
20x: 92.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700015F17Rik	C	A	5: 5,452,019	W144C	probably benign	Het
Aak1	T	G	6: 86,956,336	S430A	unknown	Het
Abhd16a	T	A	17: 35,101,015	F337L	probably benign	Het
Acsl1	C	A	8: 46,530,986	A514E	probably benign	Het
Adam29	T	C	8: 55,873,089	Y110C	probably damaging	Het
Adamts12	C	A	15: 11,270,599	Q647K	probably benign	Het
Adamts20	T	A	15: 94,404,010	H27L	probably benign	Het
Adamtsl1	A	G	4: 86,342,411	E961G	possibly damaging	Het
AU022751	T	C	X: 6,082,763	E66G	probably benign	Het
AW011738	T	C	4: 156,203,540		probably benign	Het
Bcl9	T	C	3: 97,205,144	M1332V	probably damaging	Het
Birc6	T	C	17: 74,565,982	I412T	probably damaging	Het
Cav1	A	T	6: 17,339,332	I139F	probably damaging	Het
Col4a4	A	C	1: 82,466,600		probably null	Het
Coro2a	A	T	4: 46,539,138	*544R	probably null	Het
Cox15	C	T	19: 43,746,785	R181H	probably benign	Het
Ddx23	G	A	15: 98,650,718	R370W	possibly damaging	Het
Dennd2c	A	G	3: 103,133,252	N278D	probably benign	Het
Dgkh	T	C	14: 78,616,505	I265V	probably damaging	Het
Diexf	T	A	1: 193,118,309	K401I	probably damaging	Het
Dpp3	A	T	19: 4,917,860		probably benign	Het
Drc7	G	A	8: 95,071,253	R433H	possibly damaging	Het
Ece1	A	G	4: 137,938,763	K306R	probably benign	Het
Eif4b	T	C	15: 102,088,976	S309P	unknown	Het
Eml3	G	A	19: 8,937,143	V507M	probably benign	Het
Fat1	C	T	8: 45,044,228	T4250M	possibly damaging	Het
Fezf1	T	G	6: 23,246,907	I309L	probably damaging	Het
Fsip2	T	A	2: 82,986,733	L4270H	probably damaging	Het
Gabra4	T	C	5: 71,638,237	K206E	probably damaging	Het
Gfm1	A	G	3: 67,456,585	K465E	probably benign	Het
Gpld1	A	T	13: 24,943,710	I32L	possibly damaging	Het
Hist1h4k	A	T	13: 21,750,512		probably null	Het
Hoxd12	A	G	2: 74,675,513	T143A	probably benign	Het
Hoxd12	A	G	2: 74,675,531	T149A	probably benign	Het
Hspg2	T	A	4: 137,540,230	S2050T	probably damaging	Het
Il31ra	T	A	13: 112,541,222	N287I	probably damaging	Het
Itga4	G	A	2: 79,313,844		probably null	Het
Itpr2	A	G	6: 146,240,354	V1730A	probably benign	Het
Klkb1	T	A	8: 45,275,477	Q415L	probably benign	Het
Lcn10	A	G	2: 25,684,335	Y118C	probably damaging	Het
Lgi3	T	C	14: 70,536,268	V294A	probably damaging	Het
Lrp5	C	T	19: 3,610,131	V978I	probably benign	Het
Naaa	A	G	5: 92,278,035	V33A	probably benign	Het
Nckap5l	A	G	15: 99,427,261	F454L	probably damaging	Het
Nol10	A	G	12: 17,348,554	M1V	probably null	Het
Olfm1	G	A	2: 28,222,662		probably null	Het
Olfr520	G	T	7: 99,735,860	R239L	possibly damaging	Het
Olfr784	T	A	10: 129,387,876	M81K	probably benign	Het
Olfr849	T	C	9: 19,441,351	L146P	possibly damaging	Het
Osbp2	T	C	11: 3,726,333		probably null	Het
Osbpl1a	T	A	18: 12,905,194	Q269L	probably benign	Het
Papss2	T	A	19: 32,638,968	C191*	probably null	Het
Pbxip1	A	T	3: 89,447,677		probably null	Het
Pdp1	A	G	4: 11,962,074	I79T	probably benign	Het
Peg10	A	G	6: 4,755,778	Y118C	probably damaging	Het
Pnisr	A	G	4: 21,873,612	T452A	probably benign	Het
Polr2a	A	T	11: 69,735,375	Y1612N	unknown	Het
Polr3c	A	T	3: 96,719,298	L270H	probably damaging	Het
Prr11	A	T	11: 87,106,042	L32*	probably null	Het
Ptgfr	T	A	3: 151,835,194	T226S	probably benign	Het
Ptprz1	T	A	6: 23,007,355	V1639E	probably damaging	Het
Rps6kb1	A	G	11: 86,532,821	V111A	possibly damaging	Het
Sfxn1	A	T	13: 54,093,933	I226F	probably damaging	Het
Sh3pxd2a	A	G	19: 47,267,508	S924P	probably benign	Het
Slc16a5	A	T	11: 115,469,368	I126F	probably damaging	Het
Slc8a3	G	T	12: 81,314,446	T533N	probably damaging	Het
Snrnp40	C	G	4: 130,378,043		probably null	Het
Spata3	A	C	1: 86,022,061		probably benign	Het
St8sia6	A	G	2: 13,792,812	S48P	probably benign	Het
Stra6l	A	T	4: 45,882,698	R470*	probably null	Het
Stradb	A	C	1: 58,991,105	N173H	probably benign	Het
Sugp1	T	A	8: 70,071,540	D598E	probably benign	Het
Tekt2	T	C	4: 126,322,817		probably null	Het
Tiam2	C	T	17: 3,514,725	R1413C	possibly damaging	Het
Tmprss2	T	C	16: 97,569,062	S301G	probably benign	Het
Tnrc18	A	T	5: 142,776,324	N515K	unknown	Het
Togaram1	A	G	12: 64,966,935	Y320C	probably damaging	Het
Trim41	A	G	11: 48,807,492	V549A	probably damaging	Het
Ugt2b1	T	A	5: 86,917,841	L446F	probably damaging	Het
Unc5b	T	C	10: 60,772,569	T621A	probably benign	Het
Vmn1r214	C	A	13: 23,035,324	H329Q	possibly damaging	Het
Vmn2r109	T	A	17: 20,553,810	K428*	probably null	Het
Vmn2r81	A	C	10: 79,293,494	I740L	probably damaging	Het
Wdfy3	A	G	5: 101,941,492	L612P	probably damaging	Het
Zfp109	G	A	7: 24,228,736	T424M	probably damaging	Het

Other mutations in Zfp735

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Zfp735	APN	11	73711366	missense	possibly damaging	0.86
IGL00798:Zfp735	APN	11	73711560	missense	possibly damaging	0.72
IGL01642:Zfp735	APN	11	73710479	missense	possibly damaging	0.73
IGL01684:Zfp735	APN	11	73690365	missense	possibly damaging	0.86
IGL02096:Zfp735	APN	11	73711428	missense	probably benign	0.01
IGL02238:Zfp735	APN	11	73710493	missense	probably benign	0.00
IGL02505:Zfp735	APN	11	73689800	missense	probably benign	0.03
IGL02740:Zfp735	APN	11	73710586	missense	possibly damaging	0.53
IGL02957:Zfp735	APN	11	73710929	missense	probably benign	0.00
bananaquit	UTSW	11	73710586	nonsense	probably null
bescher	UTSW	11	73712153	missense	possibly damaging	0.93
Galvanic	UTSW	11	73711678	nonsense	probably null
grassquit	UTSW	11	73712203	missense	possibly damaging	0.66
R0114:Zfp735	UTSW	11	73710662	missense	probably benign	0.33
R0217:Zfp735	UTSW	11	73711286	missense	possibly damaging	0.73
R0943:Zfp735	UTSW	11	73712083	missense	probably benign	0.04
R1421:Zfp735	UTSW	11	73710697	missense	probably benign
R1460:Zfp735	UTSW	11	73712333	missense	possibly damaging	0.73
R1493:Zfp735	UTSW	11	73710479	missense	possibly damaging	0.73
R1517:Zfp735	UTSW	11	73710644	missense	probably benign
R1676:Zfp735	UTSW	11	73711475	missense	possibly damaging	0.53
R1709:Zfp735	UTSW	11	73711763	missense	probably benign	0.01
R1871:Zfp735	UTSW	11	73710586	nonsense	probably null
R2219:Zfp735	UTSW	11	73711025	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73711396	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73711397	nonsense	probably null
R3552:Zfp735	UTSW	11	73711241	nonsense	probably null
R3856:Zfp735	UTSW	11	73711456	missense	probably benign	0.01
R3925:Zfp735	UTSW	11	73711124	missense	probably benign	0.33
R4572:Zfp735	UTSW	11	73689785	missense	probably benign	0.02
R4585:Zfp735	UTSW	11	73689724	missense	possibly damaging	0.51
R4586:Zfp735	UTSW	11	73689724	missense	possibly damaging	0.51
R4619:Zfp735	UTSW	11	73711205	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73711855	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73711856	missense	probably damaging	0.98
R5435:Zfp735	UTSW	11	73712113	missense	possibly damaging	0.72
R5489:Zfp735	UTSW	11	73710593	nonsense	probably null
R5516:Zfp735	UTSW	11	73710814	missense	probably benign
R5654:Zfp735	UTSW	11	73712138	missense	possibly damaging	0.71
R5990:Zfp735	UTSW	11	73690348	missense	possibly damaging	0.70
R6332:Zfp735	UTSW	11	73711678	nonsense	probably null
R6427:Zfp735	UTSW	11	73690314	missense	possibly damaging	0.73
R6460:Zfp735	UTSW	11	73711652	missense	probably benign	0.33
R6820:Zfp735	UTSW	11	73688957	start codon destroyed	probably null	0.01
R6831:Zfp735	UTSW	11	73710608	missense	probably damaging	1.00
R6833:Zfp735	UTSW	11	73710608	missense	probably damaging	1.00
R6834:Zfp735	UTSW	11	73710608	missense	probably damaging	1.00
R6897:Zfp735	UTSW	11	73711054	missense	probably benign	0.08
R6941:Zfp735	UTSW	11	73690333	missense	probably benign	0.33
R7335:Zfp735	UTSW	11	73711553	missense	possibly damaging	0.47
R7366:Zfp735	UTSW	11	73712153	missense	possibly damaging	0.93
R7474:Zfp735	UTSW	11	73711176	missense	possibly damaging	0.72
R7487:Zfp735	UTSW	11	73690328	missense	possibly damaging	0.53
R7583:Zfp735	UTSW	11	73711107	missense	possibly damaging	0.86
R7866:Zfp735	UTSW	11	73710803	missense	probably benign	0.00
R8005:Zfp735	UTSW	11	73712314	nonsense	probably null
R8500:Zfp735	UTSW	11	73710985	missense	possibly damaging	0.53
R8551:Zfp735	UTSW	11	73712296	missense	probably benign	0.06
R8754:Zfp735	UTSW	11	73712174	missense	possibly damaging	0.85
R8769:Zfp735	UTSW	11	73690301	missense	possibly damaging	0.53
R8794:Zfp735	UTSW	11	73712203	missense	possibly damaging	0.66
R8835:Zfp735	UTSW	11	73710866	missense	possibly damaging	0.53
R8869:Zfp735	UTSW	11	73711684	missense	possibly damaging	0.53
R8969:Zfp735	UTSW	11	73711873	missense	possibly damaging	0.83
R9072:Zfp735	UTSW	11	73712234	missense	probably benign	0.21
R9073:Zfp735	UTSW	11	73712234	missense	probably benign	0.21
R9193:Zfp735	UTSW	11	73689774	missense	possibly damaging	0.71
R9355:Zfp735	UTSW	11	73711536	missense	probably benign	0.01
R9414:Zfp735	UTSW	11	73711197	nonsense	probably null
R9456:Zfp735	UTSW	11	73711577	missense	possibly damaging	0.53
R9573:Zfp735	UTSW	11	73712110	missense	possibly damaging	0.67
R9647:Zfp735	UTSW	11	73689774	missense	probably damaging	0.98
R9710:Zfp735	UTSW	11	73710980	missense	possibly damaging	0.86
Z1176:Zfp735	UTSW	11	73710815	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ATGGAAATCATGTATGCAATGTGGC -3'
(R):5'- AATCATGTAAATGATTCCCCATGC -3'

Sequencing Primer
(F):5'- CAAGTGCTTTGCTCAGTACTCAG -3'
(R):5'- AGGACTGTGTGAATGATTTCCCAC -3'

Posted On

2014-07-14