Mutagenetix > Incidental Mutation

Incidental Mutation 'R1932:Tnc'

215449

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

TN, TN-C, hexabrachion, tenascin-C, C130033P17Rik, cytotactin, Hxb

MMRRC Submission

039950-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1932 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63959785-64047015 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to T at 63993025 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

probably null
Transcript: ENSMUST00000030056

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000107372

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000107377

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.6%
20x: 93.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg4	T	A	9: 44,279,394 (GRCm38)	Q278H	probably benign	Het
Ace3	T	C	11: 106,004,610 (GRCm38)		probably null	Het
Aco1	T	C	4: 40,176,499 (GRCm38)	V221A	probably damaging	Het
Adamts20	T	A	15: 94,404,010 (GRCm38)	H27L	probably benign	Het
Adamts8	A	G	9: 30,956,512 (GRCm38)	D544G	probably benign	Het
Angptl4	C	A	17: 33,781,275 (GRCm38)	E40*	probably null	Het
Arsi	G	A	18: 60,916,651 (GRCm38)	G202E	probably benign	Het
Atf7ip2	T	A	16: 10,241,703 (GRCm38)	I369N	possibly damaging	Het
Atp6ap1l	T	A	13: 90,883,687 (GRCm38)	Y292F	probably damaging	Het
Atp6v1b2	A	T	8: 69,102,807 (GRCm38)	K217*	probably null	Het
Bid	C	T	6: 120,897,255 (GRCm38)	A110T	possibly damaging	Het
Blvra	T	A	2: 127,095,148 (GRCm38)	W174R	probably damaging	Het
Catsperg1	C	T	7: 29,198,143 (GRCm38)	G239R	probably damaging	Het
Ccdc157	G	T	11: 4,146,549 (GRCm38)	A400D	probably damaging	Het
Chd2	G	T	7: 73,454,445 (GRCm38)	P1298T	probably damaging	Het
CK137956	G	A	4: 127,946,858 (GRCm38)	L352F	possibly damaging	Het
Col22a1	G	A	15: 71,870,140 (GRCm38)	P503S	unknown	Het
Cox15	C	T	19: 43,746,785 (GRCm38)	R181H	probably benign	Het
Crem	C	T	18: 3,299,284 (GRCm38)	G47R	probably benign	Het
Crygs	T	A	16: 22,806,554 (GRCm38)	T46S	probably benign	Het
Cts8	T	A	13: 61,253,615 (GRCm38)	H62L	probably damaging	Het
Cyp4a32	T	A	4: 115,611,277 (GRCm38)	F319I	possibly damaging	Het
Dchs1	T	C	7: 105,765,902 (GRCm38)	S692G	probably damaging	Het
Ddx23	G	A	15: 98,650,718 (GRCm38)	R370W	possibly damaging	Het
Defa29	A	T	8: 21,326,849 (GRCm38)	S43T	probably damaging	Het
Dnajc14	A	G	10: 128,816,792 (GRCm38)	D573G	probably damaging	Het
Drd5	A	G	5: 38,319,976 (GRCm38)	Y104C	probably benign	Het
Efcab7	C	T	4: 99,911,018 (GRCm38)	P102L	probably damaging	Het
Efhc1	A	G	1: 20,967,400 (GRCm38)	Y267C	probably damaging	Het
Eif2ak4	T	C	2: 118,448,486 (GRCm38)	Y243H	probably damaging	Het
Gfm2	T	A	13: 97,141,967 (GRCm38)	I7N	probably damaging	Het
Gmds	A	G	13: 32,127,997 (GRCm38)	F150L	possibly damaging	Het
Gp2	T	C	7: 119,454,232 (GRCm38)	T169A	possibly damaging	Het
Grb14	A	G	2: 64,912,802 (GRCm38)	F508L	probably damaging	Het
Hdac5	T	A	11: 102,195,872 (GRCm38)		probably benign	Het
Heatr1	T	A	13: 12,435,185 (GRCm38)	M620K	probably damaging	Het
Herc3	A	G	6: 58,876,793 (GRCm38)	E608G	probably damaging	Het
Hoxb4	T	C	11: 96,320,041 (GRCm38)	Y156H	probably damaging	Het
Ifi205	T	A	1: 174,028,414 (GRCm38)	I17F	possibly damaging	Het
Il2rb	A	T	15: 78,491,777 (GRCm38)	S25T	possibly damaging	Het
Kansl1	T	C	11: 104,335,097 (GRCm38)	T998A	probably damaging	Het
Kcna6	G	T	6: 126,738,488 (GRCm38)	H479Q	probably benign	Het
Kif2a	T	C	13: 106,978,091 (GRCm38)	K350R	probably benign	Het
Lct	G	T	1: 128,294,161 (GRCm38)	A1547E	probably damaging	Het
Lhx9	T	A	1: 138,842,009 (GRCm38)		probably benign	Het
Lingo1	T	A	9: 56,619,650 (GRCm38)	I552F	possibly damaging	Het
Lrp4	G	A	2: 91,497,355 (GRCm38)	W1516*	probably null	Het
Lrp5	C	T	19: 3,610,131 (GRCm38)	V978I	probably benign	Het
Ltbp1	T	A	17: 75,313,034 (GRCm38)	D719E	probably benign	Het
Ltbp4	A	G	7: 27,307,766 (GRCm38)		probably null	Het
Macf1	A	G	4: 123,452,037 (GRCm38)	I1326T	probably damaging	Het
Manba	T	C	3: 135,544,740 (GRCm38)	F376S	probably benign	Het
Mink1	T	C	11: 70,608,428 (GRCm38)		probably null	Het
Nfatc2ip	C	T	7: 126,384,992 (GRCm38)	V410I	probably damaging	Het
Nlrp1b	A	T	11: 71,182,138 (GRCm38)	I293N	probably damaging	Het
Olfr432	T	C	1: 174,050,678 (GRCm38)	Y102H	probably damaging	Het
Otol1	A	C	3: 70,028,104 (GRCm38)	E476D	probably benign	Het
Pcdhb4	C	T	18: 37,309,541 (GRCm38)	P635S	probably benign	Het
Pfkfb4	T	A	9: 108,999,169 (GRCm38)	F91I	probably damaging	Het
Polq	G	A	16: 37,062,304 (GRCm38)	R1610Q	possibly damaging	Het
Polr3c	A	T	3: 96,719,298 (GRCm38)	L270H	probably damaging	Het
Ppp1r15b	C	T	1: 133,131,625 (GRCm38)		probably benign	Het
Prkca	T	G	11: 108,192,149 (GRCm38)	D90A	probably benign	Het
Sall3	T	C	18: 80,969,753 (GRCm38)	D1156G	probably benign	Het
Scn7a	A	T	2: 66,676,102 (GRCm38)	L1481H	probably damaging	Het
Selenon	A	T	4: 134,544,618 (GRCm38)	I292N	probably damaging	Het
Sema6d	T	A	2: 124,659,886 (GRCm38)		probably null	Het
Sgpp1	G	T	12: 75,716,179 (GRCm38)	Y409*	probably null	Het
Sh3pxd2a	A	G	19: 47,267,508 (GRCm38)	S924P	probably benign	Het
Slc25a13	A	G	6: 6,042,264 (GRCm38)	V638A	probably benign	Het
Snhg11	T	C	2: 158,376,826 (GRCm38)		probably benign	Het
Sorbs2	A	G	8: 45,796,352 (GRCm38)	Q800R	probably benign	Het
Srf	C	A	17: 46,549,986 (GRCm38)	G401C	probably damaging	Het
Stradb	A	C	1: 58,991,105 (GRCm38)	N173H	probably benign	Het
Swap70	C	T	7: 110,279,263 (GRCm38)	A480V	possibly damaging	Het
Sycp2	A	G	2: 178,381,957 (GRCm38)	V422A	probably damaging	Het
Taf4	G	A	2: 179,932,029 (GRCm38)	T682M	probably damaging	Het
Tet3	T	C	6: 83,404,379 (GRCm38)	N269S	possibly damaging	Het
Thap12	A	T	7: 98,716,838 (GRCm38)	I738F	possibly damaging	Het
Tiam2	C	T	17: 3,514,725 (GRCm38)	R1413C	possibly damaging	Het
Tmem229a	A	T	6: 24,955,011 (GRCm38)	F248Y	probably damaging	Het
Tmprss7	G	A	16: 45,684,593 (GRCm38)	Q145*	probably null	Het
Tonsl	A	T	15: 76,624,597 (GRCm38)	Y21N	probably damaging	Het
Tpr	A	G	1: 150,421,663 (GRCm38)	D1009G	probably benign	Het
Trpm2	G	A	10: 77,941,158 (GRCm38)	A435V	probably damaging	Het
Ubr3	T	A	2: 69,953,476 (GRCm38)		probably null	Het
Vcan	T	C	13: 89,705,534 (GRCm38)	N436D	possibly damaging	Het
Vmn2r104	T	A	17: 20,040,769 (GRCm38)	Y464F	probably damaging	Het
Vmn2r44	T	A	7: 8,367,982 (GRCm38)	R688S	probably benign	Het
Wdr74	G	T	19: 8,737,947 (GRCm38)	V157L	probably benign	Het
Wnt7a	T	A	6: 91,394,548 (GRCm38)	D144V	probably benign	Het
Zfp106	C	T	2: 120,531,681 (GRCm38)	A986T	possibly damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	64,016,824 (GRCm38)	splice site	probably benign
IGL00531:Tnc	APN	4	63,971,153 (GRCm38)	splice site	probably benign
IGL00674:Tnc	APN	4	63,965,607 (GRCm38)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	64,017,334 (GRCm38)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	64,000,080 (GRCm38)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	64,013,077 (GRCm38)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,982,875 (GRCm38)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	64,014,054 (GRCm38)	splice site	probably benign
IGL01411:Tnc	APN	4	64,000,722 (GRCm38)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	64,006,419 (GRCm38)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,970,408 (GRCm38)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,970,307 (GRCm38)	splice site	probably benign
IGL01669:Tnc	APN	4	64,000,701 (GRCm38)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	64,008,740 (GRCm38)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,966,672 (GRCm38)	splice site	probably benign
IGL02100:Tnc	APN	4	64,000,161 (GRCm38)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	64,015,072 (GRCm38)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,965,579 (GRCm38)	splice site	probably benign
IGL02712:Tnc	APN	4	63,975,256 (GRCm38)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	64,015,101 (GRCm38)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	64,000,107 (GRCm38)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,976,478 (GRCm38)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,971,224 (GRCm38)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	64,014,033 (GRCm38)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,967,306 (GRCm38)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	64,017,615 (GRCm38)	nonsense	probably null
tancredo	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
BB009:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
BB019:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
P0020:Tnc	UTSW	4	64,008,857 (GRCm38)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	64,017,736 (GRCm38)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,970,420 (GRCm38)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	64,017,442 (GRCm38)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	64,007,694 (GRCm38)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	64,000,159 (GRCm38)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	64,020,455 (GRCm38)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	64,008,734 (GRCm38)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,962,082 (GRCm38)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	64,020,468 (GRCm38)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	64,018,120 (GRCm38)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	64,008,859 (GRCm38)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,965,695 (GRCm38)	splice site	probably benign
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,964,754 (GRCm38)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	64,007,684 (GRCm38)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	64,006,384 (GRCm38)	missense	probably benign
R1750:Tnc	UTSW	4	63,972,735 (GRCm38)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	64,018,096 (GRCm38)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,999,931 (GRCm38)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.00
R1941:Tnc	UTSW	4	64,014,964 (GRCm38)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,984,630 (GRCm38)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,964,621 (GRCm38)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,995,666 (GRCm38)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,975,238 (GRCm38)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	64,014,963 (GRCm38)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	64,020,519 (GRCm38)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	64,008,710 (GRCm38)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	64,014,951 (GRCm38)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	64,016,924 (GRCm38)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,970,351 (GRCm38)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,995,672 (GRCm38)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,995,745 (GRCm38)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,995,639 (GRCm38)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,967,343 (GRCm38)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	64,017,620 (GRCm38)	nonsense	probably null
R4957:Tnc	UTSW	4	63,976,556 (GRCm38)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	64,006,248 (GRCm38)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,984,489 (GRCm38)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	64,006,502 (GRCm38)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	64,017,986 (GRCm38)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,975,229 (GRCm38)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,967,215 (GRCm38)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,967,252 (GRCm38)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,971,278 (GRCm38)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
R5237:Tnc	UTSW	4	63,962,096 (GRCm38)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,993,206 (GRCm38)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,964,730 (GRCm38)	nonsense	probably null
R5346:Tnc	UTSW	4	64,008,655 (GRCm38)	missense	probably benign
R5409:Tnc	UTSW	4	64,007,417 (GRCm38)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,966,536 (GRCm38)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	64,013,925 (GRCm38)	splice site	probably null
R5518:Tnc	UTSW	4	64,017,679 (GRCm38)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	64,008,709 (GRCm38)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	64,006,422 (GRCm38)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	64,007,730 (GRCm38)	splice site	probably null
R5686:Tnc	UTSW	4	64,008,795 (GRCm38)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	64,013,214 (GRCm38)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	64,018,166 (GRCm38)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,970,352 (GRCm38)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	64,008,796 (GRCm38)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R6416:Tnc	UTSW	4	64,007,816 (GRCm38)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,995,598 (GRCm38)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,982,745 (GRCm38)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,984,589 (GRCm38)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	64,013,128 (GRCm38)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,971,155 (GRCm38)	splice site	probably null
R7317:Tnc	UTSW	4	63,972,722 (GRCm38)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,971,232 (GRCm38)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,964,762 (GRCm38)	splice site	probably null
R7382:Tnc	UTSW	4	64,014,043 (GRCm38)	nonsense	probably null
R7399:Tnc	UTSW	4	64,020,657 (GRCm38)	start gained	probably benign
R7479:Tnc	UTSW	4	64,017,628 (GRCm38)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
R7947:Tnc	UTSW	4	64,017,343 (GRCm38)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	64,000,724 (GRCm38)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	64,008,746 (GRCm38)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,984,657 (GRCm38)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,976,469 (GRCm38)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	64,008,763 (GRCm38)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	64,017,479 (GRCm38)	missense	probably benign
R8340:Tnc	UTSW	4	64,007,799 (GRCm38)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,967,274 (GRCm38)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	64,017,446 (GRCm38)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,962,076 (GRCm38)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	64,006,264 (GRCm38)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,993,059 (GRCm38)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	64,008,850 (GRCm38)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	64,017,094 (GRCm38)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	64,000,010 (GRCm38)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,970,385 (GRCm38)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,972,736 (GRCm38)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	64,020,449 (GRCm38)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,995,705 (GRCm38)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,966,584 (GRCm38)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	64,007,808 (GRCm38)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	64,014,949 (GRCm38)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,971,175 (GRCm38)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	64,007,363 (GRCm38)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	64,018,012 (GRCm38)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	64,007,426 (GRCm38)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,960,544 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCATTTAGGTGGCTGTCAGAAG -3'
(R):5'- TGTAACTCCTAAGAGCTTCAATCTCTC -3'

Sequencing Primer
(F):5'- GTGGCTGTCAGAAGAAAATTTTG -3'
(R):5'- TAAGAGCTTCAATCTCTCCTGGACAG -3'

Posted On

2014-07-14