Incidental Mutation 'R0057:Tas2r135'
| ID |
216069 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Tas2r135
|
| Ensembl Gene |
ENSMUSG00000056203 |
| Gene Name |
taste receptor, type 2, member 135 |
| Synonyms |
mt2r38, Tas2r35 |
| MMRRC Submission |
038351-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.055)
|
| Stock # |
R0057 (G1)
|
| Quality Score |
59 |
|
Status
|
Validated
|
| Chromosome |
6 |
| Chromosomal Location |
42382463-42383428 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 42383354 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 298
(T298A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000070247
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000070178]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000070178
AA Change: T298A
PolyPhen 2
Score 0.072 (Sensitivity: 0.94; Specificity: 0.84)
|
| SMART Domains |
Protein: ENSMUSP00000070247
Gene: ENSMUSG00000056203
AA Change: T298A
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:TAS2R
|
22 |
320 |
1.3e-63 |
PFAM |
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.9%
- 10x: 97.7%
- 20x: 95.9%
|
| Validation Efficiency |
100% (50/50) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 28 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam23 |
T |
A |
1: 63,610,078 (GRCm39) |
H693Q |
probably damaging |
Het |
| Ap5z1 |
T |
C |
5: 142,456,144 (GRCm39) |
|
probably benign |
Het |
| Bloc1s6 |
T |
A |
2: 122,586,141 (GRCm39) |
|
probably benign |
Het |
| Caskin1 |
A |
G |
17: 24,723,870 (GRCm39) |
N886S |
probably damaging |
Het |
| Ctse |
G |
T |
1: 131,591,109 (GRCm39) |
D97Y |
probably damaging |
Het |
| Dcaf11 |
T |
C |
14: 55,806,767 (GRCm39) |
V490A |
probably benign |
Het |
| Dctn1 |
A |
G |
6: 83,156,874 (GRCm39) |
H7R |
probably benign |
Het |
| Dscam |
A |
C |
16: 96,474,936 (GRCm39) |
W1209G |
probably damaging |
Het |
| Fcsk |
C |
T |
8: 111,620,400 (GRCm39) |
|
probably benign |
Het |
| Gna11 |
A |
G |
10: 81,366,774 (GRCm39) |
M312T |
probably benign |
Het |
| Hacd2 |
T |
A |
16: 34,895,997 (GRCm39) |
V105D |
probably damaging |
Het |
| Htra4 |
C |
A |
8: 25,528,824 (GRCm39) |
V23L |
probably benign |
Het |
| Marf1 |
C |
T |
16: 13,960,398 (GRCm39) |
A549T |
probably damaging |
Het |
| Msh4 |
C |
T |
3: 153,575,318 (GRCm39) |
A686T |
probably benign |
Het |
| Nbas |
T |
A |
12: 13,440,958 (GRCm39) |
M1096K |
probably benign |
Het |
| Or5m10b |
C |
A |
2: 85,699,597 (GRCm39) |
Y220* |
probably null |
Het |
| Ppp1r16a |
C |
T |
15: 76,574,999 (GRCm39) |
|
probably benign |
Het |
| Prlr |
A |
G |
15: 10,328,509 (GRCm39) |
Y328C |
probably damaging |
Het |
| Ros1 |
C |
T |
10: 52,056,287 (GRCm39) |
V68I |
probably benign |
Het |
| Shmt2 |
G |
A |
10: 127,356,917 (GRCm39) |
T31M |
possibly damaging |
Het |
| Snapc1 |
C |
T |
12: 74,021,806 (GRCm39) |
R81C |
probably damaging |
Het |
| Tmem175 |
C |
T |
5: 108,787,428 (GRCm39) |
H92Y |
probably damaging |
Het |
| Top3a |
C |
T |
11: 60,631,510 (GRCm39) |
A951T |
probably benign |
Het |
| Trpc4ap |
T |
C |
2: 155,482,406 (GRCm39) |
E528G |
possibly damaging |
Het |
| Trpm6 |
T |
C |
19: 18,764,119 (GRCm39) |
C242R |
probably benign |
Het |
| Vwa7 |
G |
A |
17: 35,243,523 (GRCm39) |
S710N |
possibly damaging |
Het |
| Zfa-ps |
A |
T |
10: 52,421,202 (GRCm39) |
|
noncoding transcript |
Het |
| Zfp770 |
T |
A |
2: 114,027,713 (GRCm39) |
R119* |
probably null |
Het |
|
| Other mutations in Tas2r135 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01357:Tas2r135
|
APN |
6 |
42,383,078 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01395:Tas2r135
|
APN |
6 |
42,382,846 (GRCm39) |
nonsense |
probably null |
|
|
IGL02479:Tas2r135
|
APN |
6 |
42,382,685 (GRCm39) |
nonsense |
probably null |
|
|
IGL02526:Tas2r135
|
APN |
6 |
42,383,214 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02806:Tas2r135
|
APN |
6 |
42,383,382 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02982:Tas2r135
|
APN |
6 |
42,383,187 (GRCm39) |
missense |
probably benign |
|
|
IGL03057:Tas2r135
|
APN |
6 |
42,378,061 (GRCm39) |
unclassified |
probably benign |
|
|
R0104:Tas2r135
|
UTSW |
6 |
42,383,258 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
R1412:Tas2r135
|
UTSW |
6 |
42,382,768 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4517:Tas2r135
|
UTSW |
6 |
42,383,013 (GRCm39) |
missense |
probably benign |
|
|
R4629:Tas2r135
|
UTSW |
6 |
42,383,160 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5788:Tas2r135
|
UTSW |
6 |
42,382,531 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6021:Tas2r135
|
UTSW |
6 |
42,383,321 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6586:Tas2r135
|
UTSW |
6 |
42,382,952 (GRCm39) |
missense |
probably benign |
0.18 |
|
R7180:Tas2r135
|
UTSW |
6 |
42,382,685 (GRCm39) |
nonsense |
probably null |
|
|
R7458:Tas2r135
|
UTSW |
6 |
42,382,881 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R7850:Tas2r135
|
UTSW |
6 |
42,383,072 (GRCm39) |
missense |
probably benign |
|
|
R9113:Tas2r135
|
UTSW |
6 |
42,383,315 (GRCm39) |
missense |
probably benign |
0.00 |
|
Z1176:Tas2r135
|
UTSW |
6 |
42,383,168 (GRCm39) |
missense |
probably benign |
0.03 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGGCCCTTTCAGAACTTCGGGATG -3'
(R):5'- GAGCCCACCTTGAATCCTATGCAC -3'
Sequencing Primer
(F):5'- TTCTGACGCTGGTACTCAGT -3'
(R):5'- TGTTCCACTGCCATTCAGGC -3'
|
| Posted On |
2014-07-21 |
Incidental Mutation